Targeting CD33 for Acute Myeloid Leukemia Therapy

Abstract Objective The aim of this study was to analyze the level of CD33 expression in patients with newly diagnosed AML and determine its correlation with clinical characteristics. Methods: Samples were collected for analysis from AML patients at diagnosis. We evaluated the level of CD33 expression by flow cytometry analysis of bone marrow. Chi-square or t- tests were used to assess the association of categorical variables between the high and low CD33 expression groups. Spearman’s rho was applied when appropriate. Survival curves were generated by the Kaplan–Meier method. Results ① In this study we evaluated the level of CD33 expression in de novo patients diagnosed from November 2013 until January 2019. The mean value of 73.4% was used as the cutoff for the two groups. Statistical analysis revealed that 53 of the 86 (61.2%) AML patients were above the mean.② Although there was no statistical significance between CD33 expression level and gene mutation, FLT3 mutation (P=0.002) and NPM1 mutation (P༝0.001)were more likely to be seen in the high CD33 group. ③ The overall survival (OS) was worse in the high CD33 group (39.0 m vs. 16.7 m, x2 = 7.385, P = 0.007). Multivariate analysis showed that the high expression of CD33 was an unfavorable prognostic factor. ④ Of the 86 patients, CD33-high was closely related to the patients with normal karyotype (x2 = 4.891,P = 0.027), high white blood cell count (WBC, t = 2.804, P = 0.007), and a high ratio of primitive cells (t = 2.851, P = 0.005). Conclusions These findings provide a strong rationale for targeting CD33 in combination with chemotherapy, which can be considered a promising therapeutic strategy for AML.

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Targeting CD33 for acute myeloid leukemia therapy

BMC Cancer ◽

10.1186/s12885-021-09116-5 ◽

2022 ◽

Vol 22 (1) ◽

Author(s):

Jingjing Liu ◽

Jiayin Tong ◽

Haiping Yang

Keyword(s):

Cox Regression ◽

De Novo ◽

Statistical Significance ◽

Univariate Analysis ◽

Normal Karyotype ◽

Mean Value ◽

High Ratio ◽

Chi Square ◽

Npm1 Mutation ◽

The Mean

Abstract Background The aim of this study was to analyze the level of CD33 expression in patients with newly diagnosed AML and determine its correlation with clinical characteristics. Methods Samples were collected for analysis from AML patients at diagnosis. We evaluated the level of CD33 expression by flow cytometry analysis of bone marrow. Chi-square or t- tests were used to assess the association between the high and low CD33 expression groups. Survival curves were generated by the Kaplan-Meier and Cox regression model method. Results In this study we evaluated the level of CD33 expression in de novo patients diagnosed from November 2013 until January 2019. The mean value of 73.4% was used as the cutoff for the two groups. Statistical analysis revealed that 53 of the 86 (61.2%) AML patients were above the mean. Although there was no statistical significance between CD33 expression level and gene mutation, FLT3 mutation (P = 0.002) and NPM1 mutation (P = 0.001) were more likely to be seen in the high CD33 group. The overall survival (OS) was worse in the high CD33 group (39.0 m vs. 16.7 m, x2 = 13.06, P < 0.001). The Cox survival regression display that the CD33 is independent prognostic marker (HR =0.233,p = 0.008). Univariate analysis showed that the high expression of CD33 was an unfavorable prognostic factor. Of the 86 patients, CD33-high was closely related to the patients with normal karyotype (x2 = 4.891,P = 0.027), high white blood cell count (WBC, t = 2.804, P = 0.007), and a high ratio of primitive cells (t = 2.851, P = 0.005). Conclusions These findings provide a strong rationale for targeting CD33 in combination with chemotherapy, which can be considered a promising therapeutic strategy for AML.

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MO780SURVIVAL STUDY OF ARTERIOVENOUS FISTULAS CREATED IN A HIGH RESOLUTION HOSPITAL

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfab103.0018 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

Luis Guillermo Piccone Saponara ◽

Maria Paz Castro Fernández ◽

Nancy Giovanna Uribe Heredia ◽

Agustín Carreño Parrilla ◽

Sara Anaya Fernandez ◽

...

Keyword(s):

Overall Survival ◽

High Resolution ◽

Statistical Significance ◽

Survival Rates ◽

Vascular Pathology ◽

Categorical Variables ◽

Survival Times ◽

Cross Sectional ◽

Kaplan Meier ◽

The Mean

Abstract Background and Aims Clinical practice guidelines recommend an arteriovenous fistula (AVF) as the ideal vascular access for hemodialysis. Autologous AVFs have higher primary, assisted primary and overall survival, associated with lower morbidity and mortality compared to prosthetic AVFs. However, primary failure of AVFs function is not uncommon, above all due to the vascular pathology of patients on hemodialysis (HD). We determined the survival rates in a series of vascular accesses created in a high-resolution hospital. Method Cross-sectional study; We include all AVFs performed during the last 20 years. Demographic variables (age, sex), etiology of CKD and associated comorbidity were collected. We determine the primary, assisted and global survival times. Statistical analysis with SPSS 25.0. Categorical variables are expressed as percentages and are compared using the Chi2 test. Quantitative variables are expressed as mean ± standard deviation and the Mann Whitney Student-T/U was used to compare them. We performed a kaplan-Meier analysis determining primary, assisted and overall survival. Statistical significance for a value of p <0.05. Results 622 AVFs performed in 482 patients were reviewed. 86.8% were autologous. The mean age was 65.4±14 years; 66.6% were male. The most frequent etiologies of CKD were diabetic nephropathy (30.2%), unknown (18%), and glomerulonephritis (16.6%). 91.2% had arterial hypertension (HBP) and diabetes mellitus (DM) 47.9%. 48.7% received antiplatelet therapy and 15.6% anticoagulation prior to the creation of the AVF. 27% presented primary failure. In the survival analysis using the Kaplan Meier test, the mean time to perform angioplasty in dysfunctional AVFs was 14.6±1.37 months and to perform a thrombectomy 17.6±1.31 months. The overall survival of AVFs was 41.9±2 months. When assessing the type of AVF, we observed a longer survival for autologous (31.5±1.8) vs prosthetic (21.8±3.6) (p = 0.03 log Rank 4.73). Conclusion In our study, autologous AVFs have better survival compared to prosthetic ones. Of the AVFs created, primary survival at one year (requiring angioplasty) was 64%, assisted primary survival (requiring thrombectomy) was 42%, and overall survival 24%.

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Colonoscopy does not induce small intestinal bacterial overgrowth

Journal of Digestive Endoscopy ◽

10.4103/0976-5042.202813 ◽

2017 ◽

Vol 08 (01) ◽

pp. 12-16

Author(s):

Ioana Gabriela Moraru ◽

Dan Lucian Dumitraşcu

Keyword(s):

Statistical Significance ◽

False Negative ◽

Bacterial Overgrowth ◽

Intestinal Bacteria ◽

Small Intestinal Bacterial Overgrowth ◽

Mean Value ◽

Intestinal Bacterial Overgrowth ◽

Gastrointestinal Pathology ◽

The Mean ◽

Small Intestinal

Abstract Background and Aim: Small intestinal bacterial overgrowth (SIBO) is associated with gastrointestinal pathology and colonoscopy. This endoscopic investigation could cause changes in gut flora including the occurrence of SIBO. We looked in this study for the effect of colonoscopy (preparation and intubation) on the occurrence of SIBO. Materials and Methods: Prospective study including thirty patients with irritable bowel syndrome (IBS) diagnosed according to Rome III criteria. Two groups were designed: Twenty IBS patients that performed colonoscopy (G1) and ten IBS patients (G2) not referred to colonoscopy. All patients have been tested for the presence of SIBO using glucose hydrogen breath tests (GHBT) at the beginning of the study, on day 1. G1 patients have also been tested before colonoscopy (day 2) and 1 week after (day 9). G2 patients performed GHBT on day 1 and on day 9. Results: The peak value of expired H2 was assessed, and the mean value was calculated. There were no significant statistical differences between the mean H2 values in the 2 groups of patients on day 1. The mean level of H2 significantly decreased after preparing for colonoscopy in G1 patients (P < 0.0001). There were no significant statistical differences between the mean levels of H2 on day 2 versus day 9 in G1 patients (P = 0.176). The mean level of H2 1 week after performing colonoscopy (7.65 ppm) is higher than that obtained after preparing for it (6.3 ppm), but no statistical significance. Patients from G2 showed no statistical differences between the mean levels of H2 on day 1 versus day 9 (P = 0.6132). Patients in G1 had a significantly lower mean H2 level versus G2 patients on day 9. Conclusions: Colonoscopy does not produce SIBO. Preparing for colonoscopy influences the level of expired H2, it reduces the number of intestinal bacteria, probably trough a mechanic effect or by inflating air during the procedure. Performing GHBT too soon after colonoscopy might result in false negative results of GHBT.

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Echocardiographic Changes of Carfilzomib Therapy in Multiple Myeloma Patients

Blood ◽

10.1182/blood-2020-140090 ◽

2020 ◽

Vol 136 (Supplement 1) ◽

pp. 34-34

Author(s):

Waled Bahaj ◽

Anas Albawaliz ◽

Madeline E Begemann ◽

Anuj Shrestha ◽

Brett Sperry ◽

...

Keyword(s):

Statistical Significance ◽

Systolic Dysfunction ◽

Community Setting ◽

Categorical Variables ◽

Cardiac Complications ◽

Publicly Traded ◽

Private Company ◽

Chi Square ◽

Difficult Decision ◽

The Past

Introduction: Carfilzomib is a proteasome inhibitor that is known to be associated with cardiotoxicity. Current clinical data on Carfilzomib associated with cardiotoxicity is generated in clinical trials from which patients with severe cardiovascular comorbidities were excluded. In this study, we have reported real-word experience on outcome of cardiotoxicity in patients managed by physicians in a community setting. Methods: We performed a retrospective analysis evaluating for cardiac complications in MM patients who received Carfilzomib at our institution in the last 5 years. Pre- and post-therapy echocardiogram findings were compared. Chi-square tests were used to compare categorical variables with an α level set at 0.05 for statistical significance. Results: Among the 28 identified patients who had pre- and post- Carfilzomib therapy echocardiogram imaging, (18 patients, 64%) had at least one echocardiographic abnormality. The main changes were in heart functions (10/28), whereas systolic dysfunction is seen in 9 patients while only one patient had diastolic dysfunction, pulmonary artery pressure (6/28), and wall motion abnormalities (5/28). (11/28) patients had disease progression. We did not notice any correlation to variables such as; age, duration of therapy, dose differences, and number of lines of therapies. Furthermore, two patients were rechallenged with Carfilzomib after echocardiographic worsening; one of them tolerated the treatment well, while the other had further worsening that led to holding the therapy. Conclusion: Several echocardiographic changes could be related to Carfilzomib therapy. Our study was limited due to the sample size and the retrospective nature of the analysis. Larger studies are needed to detect and correlate more echocardiogram variables in this population. Rechallenging these patients with Carfilzomib, particularly during relapse stages, will remain a difficult decision especially if the therapy was effective. Disclosures Raza: Advisory board Incyte, Amgen, Celgene, Kite, Janseen, Takeda: Consultancy, Membership on an entity's Board of Directors or advisory committees; Moderna: Current equity holder in publicly-traded company; Drrx: Divested equity in a private or publicly-traded company in the past 24 months; Gilead, Sierra, Abbot, Acasti, Amicus: Current equity holder in private company; Received Honorarium/speaker bureu from Janseen, Celgene, Takeda: Ended employment in the past 24 months, Honoraria, Speakers Bureau.

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Post-Endodontic Treatment Flare-Up of Patients Treated By General Dentists and Endodontic Specialists

Open Access Journal of Dental Sciences ◽

10.23880/oajds-16000315 ◽

2021 ◽

Vol 6 (4) ◽

Author(s):

Mohammed Sarhan Alzahrani

Keyword(s):

Postoperative Pain ◽

Statistical Significance ◽

Lesion Size ◽

Endodontic Treatment ◽

Categorical Variables ◽

Preoperative Pain ◽

Patient Factors ◽

Chi Square ◽

Dental Practitioners ◽

General Dentists

Objective: This study aims to determine post-endodontic flare-up incidence for patients treated by endodontic specialists and general dental practitioners. Methods: The investigators measured the postoperative pain using a visual analog scale after 24 and 48 hours for patients treated endodontically by specialists and general dentists. The patient factors and operative variables were documented for every patient. The data was then analyzed using SPSS. A Chi-square test was used to evaluate a relationship between categorical variables, where a P < 0.05 was taken to indicate statistical significance. Results: In total, 143 participants were included in this study (male: n=62, 43.3%; and female: n=81, 56.7%). The incidence of post-endodontic treatment flare-ups was 14.8%. The incidence of 48 hours postoperative pain was higher (29%) with the endodontic specialists compared to the general practitioners. The factors that were associated with a higher postoperative flare-up include; patient age, smoking, preoperative pain, and periapical lesion size. Conclusion: Post-endodontic flare-up incidence in this study was within the reported range of flare-up. Patient factors are associated with the occurrence of the postoperative flare-up more than the operative factors.

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Arthroscopic Bristow: 12-Year Experience, Assessments of Safety and Effectiveness

Revista Brasileira de Ortopedia ◽

10.1055/s-0039-1697972 ◽

2019 ◽

Author(s):

Jose Carlos Garcia

Keyword(s):

Los Angeles ◽

Shoulder Instability ◽

De Novo ◽

Mean Value ◽

Anterior Shoulder Instability ◽

Simple Shoulder Test ◽

The Mean ◽

Lateral Rotation ◽

Clinically Significant

Abstract Objective The open Bristow procedure is a long established and effective method for treating anterior shoulder instability. Following the trends of minimally-invasive surgeries, these procedures were performed arthroscopically, and their outcomes were evaluated. Methods A total of 43 shoulders of patients submitted to Bristow procedures by arthroscopy, using a graft positioned horizontally and a screw, with at least two years of postoperative follow-up, were evaluated regarding quality of life, de novo dislocation index, and loss of lateral rotation. Results The mean follow-up time was of 76 months (range: 129 to 24 months). The University of California at Los Angeles (UCLA) score varied from 25.56 ± 0.50 (standard deviation [SD] = 3.25) to 33.23 ± 0.44 (SD = 2.91) (p < 0.0001). Two or more years after surgery, the mean Rowe score was of 94.25 ± 1.52 (SD = 1.34), whereas the good results standard is 75 (p < 0.0001). The mean value for the simple shoulder test was of 11.35 ± 0.21 (SD = 1.34), while the mean value of the lateral rotation loss was of 10.37° ± 1.36° (SD = 8.58°). There were no de novo dislocations.In total, there were 12 complications, 8 of which had no clinical repercussions. The clinically-significant complications included an infection six months after surgery with a potential hematogenous origin, a coracoid fracture that required an intraoperatively procedure change, and two patients with previous impingement who required synthesis material removal more than six months after surgery. Conclusion Although the arthroscopic Bristow procedure was effective in treating anterior shoulder instability, it is not a complication-free surgery.

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Two-Year Outcomes of Orbital Atherectomy Combined With Drug-Coated Balloon Angioplasty for Treatment of Heavily Calcified Femoropopliteal Lesions

Journal of Endovascular Therapy ◽

10.1177/1526602820915244 ◽

2020 ◽

Vol 27 (3) ◽

pp. 492-501 ◽

Cited By ~ 2

Author(s):

Damianos G. Kokkinidis ◽

Omar Jawaid ◽

David Cantu ◽

Brad J. Martinsen ◽

Zsuzsanna Igyarto ◽

...

Keyword(s):

Cox Regression ◽

De Novo ◽

Combined Therapy ◽

Statistical Significance ◽

Retrospective Chart Review ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Effective Combination ◽

Orbital Atherectomy ◽

Procedural Complications

Purpose: To examine whether the combination of orbital atherectomy (OA) and drug-coated balloons (DCB) can lead to superior procedural and 2-year outcomes compared with DCB only in heavily calcified femoropopliteal (FP) lesions. Materials and Methods: A retrospective chart review was conducted to identify patients treated with DCB only or OA+DCB for de novo FP lesions at a single center over a 4-year period (2014–2017). In the observation period, 113 patients met the inclusion criteria: 63 treated with DCB only (mean age 69.0±8.6 years; 62 men) vs 50 treated with OA+DCB (mean age 70.3±7.1 years; 48 men). The OA+DCB group had higher calcification rates (78% with severe calcification vs 37% in the DCB only group). Propensity score matching (PSM) was used to adjust for baseline differences between the 2 groups. Cox regression analysis was used to compare the follow-up outcomes between lesions treated with OA+DCB vs DCB only. Results: No difference in procedural complications or success was found. After PSM adjustment, the OA+DCB group was associated with lower bailout stenting rates (39.4% vs 66.7% in the DCB only group; p=0.026). The 2 groups had similar long-term outcomes, although the OA+DCB arm had a trend toward reduced TLR rates that did not reach statistical significance. The Kaplan-Meier estimates for 2-year freedom from TLR were 76.1% for the OA+DCB group vs 55.5% for the DCB only group (p=0.109). Conclusion: OA+DCB is a safe and effective combination for the treatment of calcified FP lesions. The combined therapy decreased the bailout stenting rates in the adjusted analysis. Larger cohorts and randomized trials are needed to examine OA efficacy in FP lesions.

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Pie Crust Technique of the Deep Medial Collateral Ligament in Knee Arthroscopy: Ultrasound and Anatomic Study

The Journal of Knee Surgery ◽

10.1055/s-0038-1668125 ◽

2018 ◽

Vol 32 (08) ◽

pp. 764-769

Author(s):

Guillem Claret-Garcia ◽

Jordi Montañana-Burillo ◽

Eduard Tornero-Dacasa ◽

Manel Llusá-Pérez ◽

Dragos Popescu ◽

...

Keyword(s):

Medial Collateral Ligament ◽

Statistical Significance ◽

Mean Value ◽

Saphenous Nerve ◽

Medial Compartment ◽

P Value ◽

Collateral Ligament ◽

Anatomical Dissection ◽

Ultrasound Measurements ◽

The Mean

AbstractThis article determines compartment opening of the medial articular space of the knee after pie crust (PC) technique of the medial collateral ligament (MCL) by ultrasound measurements and anatomic dissection. This is a cadaveric study of 12 specimens. Four anatomic references were marked on the skin. Distances between the femur and tibia in the internal compartment at 30 degrees of flexion were obtained with ultrasound measurements in four situations: with and without applying valgus force both prior and after the PC technique. Ultrasound measurements of the medial articular compartment were made twice and mean value was calculated. An anatomical dissection was performed and distances between the puncture marks and the infrapatellar branch of the saphenous nerve was measured. Lilliefors test of normality was applied and variables were expressed as mean and standard deviation (SD). Qualitative variables were expressed by absolute frequencies and percentages. Statistical significance was a two-tailed p-value of < 0.05. Prior to the PC technique, mean (SD) distance between the femur and tibia in the medial compartment were 14.2 (4.0) mm in basal conditions and 17.1 (3.7) mm when applying valgus force (p = 0.003). PC technique increased the mean (SD) distance by 1.9 (1.9) mm under basal conditions (p < 0.01) and 2.9 (1.6) mm when applying valgus force (p < 0.01). The infrapatellar branches of the saphenous nerve were not damaged and the mean (SD) distance between the punctures and the nerve was 9.0 (3.3) mm. The PC is a reproducible, safe, and measurable surgical technique that opens controllably the medial compartment. PC as described avoided damage to the nerve branches.

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A Retrospective Multicenter Study of 1898 Liquefied Petroleum Gas-Related Burn Patients in Eastern China From 2011 to 2015

Journal of Burn Care & Research ◽

10.1093/jbcr/iraa067 ◽

2020 ◽

Vol 41 (6) ◽

pp. 1188-1197

Author(s):

Ronghua Jin ◽

Jiaming Shao ◽

Jon Kee Ho ◽

Meirong Yu ◽

Chunmao Han

Keyword(s):

Eastern China ◽

Total Body Surface Area ◽

Length Of Hospital Stay ◽

Categorical Variables ◽

Liquefied Petroleum Gas ◽

Chi Square ◽

Burn Prevention ◽

Chi Square Test ◽

The Mean ◽

The One

Abstract Liquefied petroleum gas (LPG) is a widely used environment-friendly fuel. Previous studies have shown an increasing number of LPG-related burns. Our study was designed to evaluate the epidemiologic pattern of these injuries and provide recommendations for burn prevention. This retrospective study included all patients with LPG-related burns from eight burn centers in Zhejiang Province, China between 2011 and 2015. Database variables included patient demographics, accident characteristics, and injury characteristics. The association between different categorical variables was identified using the chi-square test. And the association between two or more means of quantitative variables was analyzed by the one-way analysis of variance or t-test. A total of 1898 patients were included, 47.31% were males and 52.69% were females. The predominant age group was 31 to 70 years (74.50%), and the majority were poorly educated and the incidence peaked from June to September. The most common place of occurrence was home (74.08%) and gas leak (96.52%) was the most common cause. The four limbs (43.33%) were the most frequently affected areas; the mean burn area was 25.19 ± 20.97% of the total body surface area and most patients (46.89%) suffered from moderate burns. The mean length of hospital stay was 17.66 ± 16.55 days and the majority of patients (89.36%) recovered with a 0.84% mortality rate. Our findings reflected that the increase in incidence rate was alarming, and the causes resulting in LPG-related burns have not gained much attention yet. Therefore, this calls for simple but strict measures aiming at each hazardous step during the use of LPG to prevent these burn injuries.

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Evolution of bipolar disorder over 12 years in a psychiatric hospital

European Psychiatry ◽

10.1016/j.eurpsy.2016.01.165 ◽

2016 ◽

Vol 33 (S1) ◽

pp. S125-S125

Author(s):

E. Ribera ◽

M. Grifell ◽

M.T. Campillo ◽

I. Ezquiaga ◽

L. Martínez ◽

...

Keyword(s):

Bipolar Disorder ◽

Early Detection ◽

Psychiatric Hospitals ◽

Categorical Variables ◽

Chi Square ◽

Number Of Patients ◽

Chi Square Test ◽

The Mean ◽

Second Period ◽

Carlos Iii

IntroductionBipolar disorder is a leading cause of hospitalization in psychiatric hospitals. It is known that early detection of bipolar disorder is associated with a better prognosis.ObjectivesThe aim of this study is to conduct a demographic analysis of patients hospitalized for bipolar disorder in a single center between 2003 to 2014.MethodsRetrospective cohort study of 1230 patients admitted with bipolar disorder diagnosis from 2003 to 2014 at Centre Assistencial Emili Mira i López of Parc Salut Mar of Barcelona. We divided the study in two periods: 2003–2008 and 2009–2014. We analyzed the following variables: frequency of admissions, age, sex and days of hospital stay, comparing both periods. Chi-square test for categorical variables and Student t test for quantitative variables were applied.ResultsThe mean ages at the first and second period are 52 and 47, respectively (P < 0.001). There are no significant differences in sex and days of hospitalization. The frequency of admissions on the first and third trimesters is higher than in the second and fourth, although the differences are not statistically significant.ConclusionsDespite the large number of patients in the study, there are limitations, such as being a retrospective study and not being adjusted for confounding factors. The average age of patients in the second period is lower than in the first. This could suggest an improvement in early detection of bipolar disorder in the last years. Further research is needed to confirm this hypothesis.Disclosure of interestLG is funded by the Instituto de Salud Carlos III(CM14/00111).

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