Repeatability and Reliability of Different Diagnostic Platforms in Normal Tear Film Evaluation
Abstract Purpose To assess the repeatability and reliability of different commercially available diagnostic platforms in the objective assessment of normal tear film parameters. Methods Thirty healthy individuals (N = 60 eyes) had their tear film parameters (Lipid layer thickness (LLT), Tear meniscus height (TMH), Non-invasive tear break up time (NIBUT)) assessed by two observers on different occasions. The three instruments that were used are- LipiView® II, IDRA ocular surface analyzer and Oculus keratograph 5M. Bland-Altman analysis and mixed effects model with maximum likelihood estimation were used to calculate intra- and interobserver variability between the instruments. Results There were no significant intraobserver differences noted in LLT values measured with Lipiview, NIBUT using Oculus & IDRA, and Oculus TMH values. Between two observers, there were significant differences in LLT measurements (mean difference of 6.60; p = 0.002) and TMH measurements (mean difference of 0.03; p = 0.0001), obtained using IDRA but not for Oculus or Lipiview. Between instruments, all the measurements (LLT, NIBUT and TMH) were significantly different (p < 0.0001 for LLT; p = 0.002 for TMH; p < 0.0001 for NIBUT). Conclusion No two dry eye diagnostic platforms can be used interchangeably for the evaluation of the normal tear film. The NIBUT and LLT measurements using Oculus and Lipiview are more reliable than IDRA, and Oculus is more reliable than IDRA for TMH assessment.