Optimization of Ligature/Bone Defect-Induced Periodontitis Model in Rats

2021 ◽  
Author(s):  
Jingyi Gao ◽  
Simin Cai ◽  
Dan Li ◽  
Zijie Wang ◽  
Minyi Ou ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Defect ◽  
Alveolar Bone ◽  
Cost Effective ◽  
Pathological Process ◽  
Defect Group ◽  
Surgical Trauma ◽  
Computed Tomographic ◽  
Periodontal Inflammation ◽  
Severe Periodontitis

Abstract Background The destruction of alveolar bone is a crucial manifestation of severe periodontitis, which stem cell-based bioengineered therapies are expected to cure. Therefore, a cost-effective, reproducible, quantifiability and easier-to-administrate animal model that mimics human periodontitis is of great importance for further endeavor. Methods In this study, we created periodontitis rat models in silk ligation group, bone defect group and bone defect/silk ligation group respectively. he clinical indexes of periodontitis were observed and recorded. The mandible was taken for micro-computed tomographic, histological and histomorphometric analysis to assess the periodontal inflammation and bone remodeling Results Obvious periodontal inflammation but slight alveolar bone resorption were observed in the ligation group, while surgical trauma was not robust enough to continually worsen the constructed bone defect area in the bone defect group. In the bone defect/ligature group, obvious and stable periodontal inflammation could be the most lasting with similar evolving pathological patterns of human periodontitis. It also exhibited enhanced clinical similarity and confirmed its superiority in quantitativeness. Conclusions The present rat model is the first study to reproduce a pathological process similar to human periodontitis with reliable stability and repeatability, manifesting a priority to previous methods. Day 9 to Day 12 is the best time for reproducing severe periodontitis syndromes with vertical bone resorption in this model.

scholarly journals Full Mouth Rehabilitation with Implant-Supported Prostheses for Severe Periodontitis: A Case Report

2010 ◽  
Vol 4 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Sompop Bencharit ◽  
Debra Schardt-Sacco ◽  
Michael B Border ◽  
Colin P Barbaro
Keyword(s):  
Dental Implants ◽  
Bone Grafting ◽  
Alveolar Bone ◽  
Primary Stability ◽  
Healing Time ◽  
Oral Rehabilitation ◽  
Implant Placement ◽  
Periodontal Inflammation ◽  
Severe Periodontitis ◽  
Full Mouth Rehabilitation

Oral rehabilitation for a patient with severe loss of alveolar bone and soft tissue resulting from severe periodontitis presents a challenge to clinicians. Replacing loosening natural teeth with fixed prostheses supported by dental implants often requires either gingival surgery or bone grafting. The outcome of the bone grafting is sometimes unpredictable and requires longer healing time and/ or multiple surgeries. The presence of periodontal inflammation and periapical lesions often delay the placement of bone grafts as well as dental implants. Here we present a clinical case of a patient undergone full mouth reconstruction with implant-supported fixed prostheses. We demonstrated that early placement of implants (three weeks after extractions) with minimal bone grafting may be an alternative to conventional bone grafting followed by implant placement. We believe that primary stability during implant placement may contribute to our success. In addition, composite resin gingival material may be indicated in cases of large fixed implant prostheses as an alternative to pink porcelain.

Extracorporeal Shock Wave Therapy Induces Alveolar Bone Regeneration

2008 ◽  
Vol 87 (7) ◽  
pp. 687-691 ◽  
Author(s):  
S. Sathishkumar ◽  
A. Meka ◽  
D. Dawson ◽  
N. House ◽  
W. Schaden ◽  
...  
Keyword(s):  
Shock Wave ◽  
Bone Resorption ◽  
Alveolar Bone ◽  
Energy Levels ◽  
Extracorporeal Shock Wave Therapy ◽  
Shock Wave Therapy ◽  
Single Episode ◽  
Periodontal Tissues ◽  
Periodontal Inflammation ◽  
Extracorporeal Shock Wave

Periodontal inflammation with alveolar bone resorption is a hallmark of periodontitis. We hypothesized that extracorporeal shock wave therapy (ESWT) could promote the regeneration of alveolar bone following Porphyromonas gingivalis-induced periodontitis in rats. Rats were infected with P. gingivalis for 10 wks, which caused alveolar bone resorption. The rats were then treated with a single episode of 100, 300, or 1000 impulses of shock wave on both cheeks at energy levels 0.1 mJ/mm2. Alveolar bone levels were determined at 0, 3, 6, and 12 wks following ESWT and compared with those in untreated controls. Infected rats treated with 300 and 1000 impulses demonstrated significantly improved alveolar bone levels at 3 wks compared with untreated controls, and the improved levels remained for at least 6 wks in most rats. The results demonstrated effective regeneration of alveolar bone by ESWT and suggested that ESWT should be evaluated as an adjunct in the regeneration of periodontal tissues following periodontal disease. Abbreviations: ESWT, extracorporeal shock wave therapy; PCR, polymerase chain-reaction.

scholarly journals Rat Model of Polymicrobial Infection, Immunity, and Alveolar Bone Resorption in Periodontal Disease

2007 ◽  
Vol 75 (4) ◽  
pp. 1704-1712 ◽  
Author(s):  
Lakshmyya Kesavalu ◽  
Sabapathi Sathishkumar ◽  
Vasudevan Bakthavatchalu ◽  
Chad Matthews ◽  
Dolph Dawson ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Periodontal Disease ◽  
Alveolar Bone ◽  
Fusobacterium Nucleatum ◽  
Polymicrobial Infection ◽  
Radiographic Measurement ◽  
Igg Antibody ◽  
Oral Infections ◽  
Periodontal Inflammation ◽  
Polymicrobial Infections

ABSTRACT One of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. In this study we hypothesized that P. gingivalis, T. denticola, and T. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in a polymicrobial infection in rats. Groups of rats were infected with either P. gingivalis, T. denticola, or T. forsythia in monomicrobial infections or with all three species in polymicrobial oral infections with or without Fusobacterium nucleatum. PCR analyses of oral microbial samples demonstrated that rats infected with one bacterium were orally colonized by each of the bacteria during the study interval, and increased serum immunoglobulin G (IgG) antibody levels substantiated the interaction of the host with the infecting bacteria. PCR analyses of the rats with polymicrobial infections demonstrated that most rats were infected with P. gingivalis, T. denticola, and T. forsythia as a consortium. Furthermore, all rats exhibited a significant increase in the level of IgG antibody to the polymicrobial consortium. Radiographic measurement of alveolar bone resorption showed that rats infected with the polymicrobial consortium with or without F. nucleatum exhibited significantly increased alveolar bone resorption compared to the resorption in uninfected control rats, as well as the resorption in rats infected with one of the microbes. These results documented that P. gingivalis, T. denticola, and T. forsythia not only exist as a consortium that is associated with chronic periodontitis but also exhibit synergistic virulence resulting in the immunoinflammatory bone resorption characteristic of periodontitis.

Evaluation of resorption and osseointegration of autogenous bone ring grafting in vertical  bone defect with simultaneous  implant placement in dogs

2020 ◽  
Author(s):  
Ke Yu ◽  
Wenjia Liu ◽  
Naichuan Su ◽  
Helin Chen ◽  
Hang Wang ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Defect ◽  
Histological Analysis ◽  
Alveolar Bone ◽  
Ct Scanning ◽  
Autogenous Bone ◽  
Control Group ◽  
Micro Ct ◽  
Implant Placement ◽  
Defect Sites

The aim of this research was to evaluate the resorption and osseointegration of an autogenous bone ring, which was grafted in a local vertical alveolar defect with simultaneous implant placement. Six Beagle dogs were enrolled in the study; their four nonadjacent mandibular premolars were extracted, and the buccal plate was removed to create bone defects in two of the four sites. Three months after extraction, Straumann implants (Ø 3.3 mm, length of 8 mm) were placed in the bone defect sites with simultaneous autogenous bone ring grafting and in the conventional extraction sites. After a 3-month healing period and a 3-month loading period, the animals were euthanized. The harvested samples were analyzed using micro-CT scanning and histological analysis. From the micro-CT measurements, the average vertical bone resorption of the bone ring was 0.23±0.03 mm, which was not significantly different from that around the conventional implant, 0.24±0.12 mm (P > 0.05). The ratio of the bone volume to the total volume of the bone ring group was 91.11±0.02, which was higher than that of the control group, 88.38±2.34 (P < 0.05). From the hard tissue section, the bone rings developed fine osseointegration with the implants and the base alveolar bone. The results suggest autogenous bone ring grafting with simultaneous implant placement can survive in a local vertical bone defect with little bone resorption and good osseointegration in dogs with strict management. A bone ring graft must be compared with guided bone generation (GBR), and a larger and longer observation must be confirmed in clinical patients.

scholarly journals Effect of Angiotensin II Receptor Blocker on Experimental Periodontitis in a Mouse Model of Marfan Syndrome

2012 ◽  
Vol 81 (1) ◽  
pp. 182-188 ◽  
Author(s):  
Naoto Suda ◽  
Keiji Moriyama ◽  
Ganjargal Ganburged
Keyword(s):  
Angiotensin Ii ◽  
Bone Resorption ◽  
Mouse Model ◽  
Marfan Syndrome ◽  
Alveolar Bone ◽  
Wild Type ◽  
Angiotensin Ii Receptor ◽  
Content Type ◽  
Severe Periodontitis ◽  
Tnf Α

Marfan syndrome is an autosomal dominant disease characterized by aneurysm and dilatation of the aortic root, tall stature, and ectopia lentis. These manifestations reflect excessive signaling of transforming growth factor beta (TGF-β). Moreover, cases are frequently associated with severe periodontitis, which is a chronic inflammation of the gingiva, periodontal ligament, and alveolar bone. Recently, angiotensin II receptor blockers (ARBs) were discovered to be an effective drug class that can prevent aortic aneurysm and dilation in Marfan syndrome by inhibiting TGF-β signaling. To investigate the effect of ARB on the progression of periodontitis, the application of a potent ARB, telmisartan, was examined in a mouse model of Marfan syndrome (MgΔ). Six-week-old male heterozygous MgΔ and wild-type mice were challenged withPorphyromonas gingivalis, which causes chronic periodontitis, with and without telmisartan application. After infection, alveolar bone resorption was measured by micro-computed tomography (μCT), and inflammatory cytokine levels were examined. Infection ofPorphyromonas gingivalisinduced alveolar bone resorption in both MgΔ and wild-type mice. The amount of resorption was significantly larger in the former than the latter. Immunoarray and enzyme-linked immunosorbent assay (ELISA) analyses demonstrated that interleukin-17 (IL-17) and tumor necrosis factor alpha (TNF-α) levels were significantly higher in infected MgΔ mice than infected wild-type mice. Telmisartan treatment significantly suppressed the alveolar bone resorption of infected MgΔ mice. Telmisartan also significantly decreased levels of TGF-β, IL-17, and TNF-α in infected MgΔ mice to levels seen in infected wild-type mice. This study suggests that ARB can prevent the severe periodontitis frequently seen in Marfan syndrome.

scholarly journals Gelatin Nanoparticles Loaded with PMX-53 Prevents Alveolar Bone Loss in Miniature Swines Model of Periodontitis

2017 ◽  
Author(s):  
Jiahui Pan ◽  
Na Li ◽  
Qiuling Tang ◽  
Gege Li ◽  
Yubo Hou ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Loss ◽  
Alveolar Bone ◽  
Treated Group ◽  
Chronic Inflammatory Disease ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Gelatin Nanoparticles ◽  
Periodontal Inflammation ◽  
Sham Control Group

Periodontitis is a chronic inflammatory disease which results in the destruction of the tooth’ s supporting tissues and the alveolar bone resorption. The complement becomes a major link between infection and inflammatory pathology including periodontitis. Gingipians as important virulence factors of P. gingivalis have the activity of C5 convertase, could cleave C5 into fully functional C5a to activate C5aR. The above process could be blocked by the C5aR antagonist (PMX-53) to suppress local periodontal inflammation, and then achieves the purpose of treatment of periodontitis. Nanoparticles incorporated within gelatin are promising carrier system for drug delivery in recent years. This study aimed to investigate whether gelatin nanoparticles loaded with PMX-53 prevents alveolar bone resorption in miniature swines model of periodontitis. Four miniature swines were placed ligatures around the maxillary and mandibular fourth premolar and first molar on both sides for seven weeks to induce periodontitis. Then, animals were assigned randomly to four groups: minocycline-treated group, gelatin with PMX53-treated group, gelatin-treated group and a sham control group. They were treated with 1ml related drugs respectively, into gingival sulcusl for 4 times at one-week intervals. We showed that local treatments with gelatin nanoparticles loaded with PMX-53 could inhibit alveolar bone loss of periodontitis. Our study revealed that gelatin nanoparticles loaded with PMX-53 prevented alveolar bone resorption miniature swines model of periodontitis. In addition, provided proof-of-concept for local targeting of gelatin nanoparticles loaded with PMX-53 as a powerful candidate for the treatment of periodontitis.

scholarly journals Cost Effectiveness of Transbronchial Needle Aspiration

1999 ◽  
Vol 6 (4) ◽  
pp. 332-335 ◽  
Author(s):  
Jennifer A Crocket ◽  
Eric YL Wong ◽  
Dale C Lien ◽  
Khanh Gia Nguyen ◽  
Michelle R Chaput ◽  
...  
Keyword(s):  
Cost Effectiveness ◽  
Cost Effective ◽  
Diagnostic Procedures ◽  
Needle Aspiration ◽  
Benign Disease ◽  
University Hospital ◽  
High Yield ◽  
Transbronchial Needle Aspiration ◽  
Computed Tomographic ◽  
The Cost

OBJECTIVE: To evaluate the yield and cost effectiveness of transbronchial needle aspiration (TBNA) in the assessment of mediastinal and/or hilar lymphadenopathy.DESIGN: Retrospective study.SETTING: A university hospital.POPULATION STUDIED: Ninety-six patients referred for bronchoscopy with computed tomographic evidence of significant mediastinal or hilar adenopathy.RESULTS: Ninety-nine patient records were reviewed. Three patients had two separate bronchoscopy procedures. TBNA was positive in 42 patients (44%) and negative in 54 patients. Of the 42 patients with a positive aspirate, 40 had malignant cytology and two had cells consistent with benign disease. The positive TBNA result altered management in 22 of 40 patients with malignant disease and one of two patients with benign disease, thereby avoiding further diagnostic procedures. The cost of these subsequent procedures was estimated at $27,335. No complications related to TBNA were documented.CONCLUSIONS: TBNA is a high-yield, safe and cost effective procedure for the diagnosis and staging of bronchogenic cancer.

scholarly journals Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hui Yu ◽  
Tianyi Zhang ◽  
Haibin Lu ◽  
Qi Ma ◽  
Dong Zhao ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Bone Volume ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Periodontal Tissues ◽  
Gingival Epithelial Cells ◽  
Trap Staining ◽  
Experimental Periodontitis ◽  
Stimulating Factor

Abstract Background Granulocyte colony-stimulating factor (G-CSF) is an important immune factor that mediates bone metabolism by regulating the functions of osteoclasts and osteoblasts. Bone loss is a serious and progressive result of periodontitis. However, the mechanisms underlying the effects of G-CSF on periodontal inflammation have yet not been completely elucidated. Here, we examined whether an anti-G-CSF antibody could inhibit bone resorption in a model of experimental periodontitis and investigated the local expression of G-CSF in periodontal tissues. Methods Experimental periodontitis was induced in mice using ligatures. The levels of G-CSF in serum and bone marrow were measured; immunofluorescence was then performed to analyze the localization and expression of G-CSF in periodontal tissues. Mice with periodontitis were administered anti-G-CSF antibody by tail vein injection to assess the inhibition of bone resorption. Three-dimensional reconstruction was performed to measure bone destruction‐related parameters via micro-computed tomography analysis. Immunofluorescence staining was used to investigate the presence of osteocalcin-positive osteoblasts; tartrate-resistant acid phosphatase (TRAP) staining was used to observe osteoclast activity in alveolar bone. Results The level of G-CSF in serum was significantly elevated in mice with periodontitis. Immunofluorescence analyses showed that G-CSF was mostly expressed in the cell membrane of gingival epithelial cells; this expression was enhanced in the periodontitis group. Additionally, systemic administration of anti-G-CSF antibody significantly inhibited alveolar bone resorption, as evidenced by improvements in bone volume/total volume, bone surface area/bone volume, trabecular thickness, trabecular spacing, and trabecular pattern factor values. Immunofluorescence analysis revealed an enhanced number of osteocalcin-positive osteoblasts, while TRAP staining revealed reduction of osteoclast activity. Conclusions G-CSF expression levels were significantly up-regulated in the serum and gingival epithelial cells. Together, anti-G-CSF antibody administration could alleviates alveolar bone resorption, suggesting that G-CSF may be one of the essential immune factors that mediate the bone loss in periodontitis.

Sign in / Sign up

Export Citation Format

Share Document