scholarly journals Electrospun Composite Nanofibers Based on Poly (ε-caprolactone) and Styrax Liquidus (Liquidambar Orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results

2021 ◽  
Author(s):  
Didem Demir ◽  
Sadin Özdemir ◽  
Seda Ceylan ◽  
M. Serkan Yalcin ◽  
Burcu Sakım ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Antimicrobial Properties ◽  
Weight Ratio ◽  
Cell Number ◽  
Mouse Embryonic Fibroblast ◽  
High Ratio ◽  
Fibrous Scaffolds ◽  
Cytotoxicity Studies ◽  
Biological Studies

Abstract In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mil. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.

scholarly journals Functionalization of 3-chloroformylcoumarin to coumarin Schiff bases using reusable catalyst: an approach to molecular docking and biological studies

2018 ◽  
Vol 5 (5) ◽  
pp. 172416 ◽  
Author(s):  
Suresh S. Kumbar ◽  
Kallappa M. Hosamani ◽  
Gangadhar C. Gouripur ◽  
Shrinivas D. Joshi
Keyword(s):  
Molecular Docking ◽  
Heterogeneous Catalysts ◽  
Vero Cells ◽  
Reusable Catalyst ◽  
Subsequent Investigation ◽  
Antimicrobial Studies ◽  
Cytotoxicity Studies ◽  
Biological Studies ◽  
Simplified Procedure

Recently, heterogeneous catalysts have been explored eximiously in the synthesis of heterocyclic compounds. Therefore, here we used solid-supported heterogeneous silica sulfuric acid as a catalyst for the synthesis of Schiff's base of 3-chloroformylcoumarin in view of simplified procedure, reusability and acceptable efficiency, which are required in organic synthesis. An efficient and facile methodology is preferred for synthesis of a class of chromeno-3-substituted derivatives ( 1a–1l ) with good yields. The molecular docking results showed excellent binding interactions with the Mycobacterium tuberculosis InhA-D148G mutant (PDB: 4DQU). The same biomolecules were screened for their in vitro anti-tubercular activity against the M.tb H37Rv strain and antimicrobial studies. Physico-chemistry, toxicity prediction with IC50 value and bioactivity score were also calculated for title compounds. Most active compounds were further tested for cytotoxicity studies and exhibited low-level cytotoxicity against Vero cells. The suggested conjugates are promising lead compounds for the subsequent investigation in search of new anti-tubercular agents. All the conjugates were obtained within the range and followed the Lipinski rule of 5, indicating more ‘drug-like’ nature.

scholarly journals Synthesis, Characterization and Biological Activity of Two New Copper (II) Complexes with N-sulfonamide Ligand

2019 ◽  
Vol 70 (11) ◽  
pp. 4060-4067
Keyword(s):  
Anticancer Agents ◽  
Dna Cleavage ◽  
Antibacterial Properties ◽  
Nuclease Activity ◽  
Normal Fibroblast ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Biological Studies

Despite the fact that a large number of chemotherapeutic anticancer agents have been discovered, cancer still remains a great cause of deaths worldwide. The purpose of our researches is to discover a new antitumor drug. In this aim, two new Cu(II) complexes, (C1) and (C2) with a new ligand, N-(5-trifluoromethyl-<1,3,4>-thiadiazole-2-yl)-benzensulfonamide(HL) were synthesized. The complexes were characterized by elemental analysis, spectral and magnetic determinations. The nuclease activity studies of the complexes confirm their capacity to cleavage the DNA molecule. Both complexes have in vitro antioxidant activity (DPPH, FRAP methods), in vitro (using xanthine /xanthine oxidase system) and in vivo (using S.cerevisiae)SOD mimetic activity.The results of MTT assay on two carcinoma cell lines (HeLa and WM35) indicate that both complexes have antitumor activity, but (C2) has a superior activity compared with (C1) and with Cisplatin. On normal fibroblast (HDFa), (C1) showed toxicity comparable with Cisplatin, but (C2) showed a lower one. Bacterial assays were also performed (by the disk diffusion method) and both complexes have antibacterial activity against S. aureus, E. coli, P. aeroginosa and B. cereus. All the biological studies are in concordance and show that both complexes have biologic activity but (C2)is much more active. Keywords: oxidative DNA cleavage, antioxidant capacity, SOD-mimetic activity, cytotoxicity, antibacterial properties

scholarly journals The fusion of erythrocytes by fatty acids, esters, retinol and α-tocopherol

1973 ◽  
Vol 136 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Q. F. Ahkong ◽  
D. Fisher ◽  
W. Tampion ◽  
J. A. Lucy
Keyword(s):  
Carboxylic Acids ◽  
Cell Fusion ◽  
Biological Significance ◽  
Cell Number ◽  
Test System ◽  
High Ratio ◽  
Cell Swelling ◽  
Mammalian Erythrocyte ◽  
Chicken Erythrocytes

1. The ability of a number of carboxylic acids, their esters, retinol and α-tocopherol to induce fusion of hen erythrocytes in vitro was investigated. 2. Some 30 different fat-soluble substances (100μg/ml) were found to cause the formation of multinucleated erythrocytes with a suspension of 3×108 erythrocytes/ml. The most effective agents induced fusion within 5–10min at 37°C; some substances required about 1h. 3. Inclusion of Dextran 60C in the test medium minimized colloid osmotic lysis caused by exogenous lipids that induce cell fusion. 4. Cell swelling, followed by cell adhesion, was then seen to precede cell fusion. 5. Fusion occurred with C10–C14 saturated carboxylic acids, with unsaturated, longer-chain carboxylic acids and their mono-esters; retinol, and to a lesser extent α-tocopherol, also caused cell fusion. 6. C6–C9, C15, C16 and C18 saturated carboxylic acids did not induce fusion within 4h; glyceryl dioleate was only weakly active, and glyceryl trioleate was inactive in the test system. 7. Fusion was facilitated by a high ratio of chemical agents to cell number and by incubation between pH5 and 6. It was inhibited by EDTA and by serum albumin. 8. Glyceryl mono-oleate caused both a similar fusion of several species of mammalian erythrocyte and the interspecific fusion of human and chicken erythrocytes. 9. The term ‘fusogenic’ is proposed to describe chemical, viral and physical agents that cause membranes to fuse. 10. The biochemical mechanisms involved and the possible biological significance of membrane fusion by fusogenic lipids are discussed.

scholarly journals Preparations of Silver/Montmorillonite Biocomposite Multilayers and Their Antifungal Activity

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 817 ◽  
Author(s):  
Simona Liliana Iconaru ◽  
Andreea Groza ◽  
George E. Stan ◽  
Daniela Predoi ◽  
Sofia Gaiaschi ◽  
...  
Keyword(s):  
Surface Morphology ◽  
Magnetron Sputtering ◽  
Antimicrobial Properties ◽  
Rf Magnetron Sputtering ◽  
Inhibitory Effect ◽  
Thin Layers ◽  
Silver Layer ◽  
Cytotoxicity Studies ◽  
Biofilm Development

In this study, the results about the influence of the surface morphology of layers based on montmorillonite (MMT) and silver (Ag) on antimicrobial properties are reported. The coating depositions were performed in the plasma of a radio frequency (RF) magnetron sputtering discharge. The studied layers were single montmorillonite layers (MMT) and silver/montmorillonite multilayers (MMT-Ag) obtained by magnetron sputtering technique with a different surface thickness. The resultant MMT-Ag biocomposite multilayers exhibited a uniform distribution of constituent elements and enhanced antimicrobial properties against fungal biofilm development. Glow-discharge optical emission spectroscopy (GDOES) analysis revealed the formation of MMT-Ag biocomposite multilayers following the deposit of a silver layer for an MMT layer that was initially deposited on a Si substrate. The surface morphology and thickness evaluation of deposited biocomposite layers were performed by scanning electron microscopy (SEM). A qualitative analysis of the chemical composition of thin layers was performed and the elements O, Ag, Mg, Fe, Al, and Si were identified in the MMT-Ag biocomposite multilayers. The in vitro antifungal assay proved that the inhibitory effect against the growth of Candida albicans ATCC 101231 CFU was more emphasized in the case of MMT-Ag biocomposite multilayers that in the case of the MMT layer. Cytotoxicity studies performed on HeLa cells showed that the tested layers did not show significant toxicity at the time intervals during which the assay was performed. On the other hand, it was observed that the MMT layers exhibited slightly higher biocompatible properties than the MMT-Ag composite layers.

scholarly journals Synthetic, structural and biological studies of organosilicon(IV) complexes of Schiff bases derived from pyrrole-2-carboxaldehyde

2010 ◽  
Vol 75 (7) ◽  
pp. 917-927 ◽  
Author(s):  
Kiran Singh ◽  
Dharam Pal
Keyword(s):  
Schiff Bases ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Molar Conductance ◽  
Azomethine Nitrogen ◽  
Spectral Studies ◽  
Central Metal Atom ◽  
Central Metal ◽  
Biological Studies

Selected new organosilicon(IV) complexes having the general formula R2SiCl[L] and R2Si[L]2 were synthesized by the reactions of Me2SiCl2 with Schiff bases [5-mercapto-4-(2-pyrrolecarboxalideneamino)-s-triazole, 5-mercapto-3-methyl-4-(2-pyrrolecarboxalidene amino)-s-triazole and 3-ethyl-5-mercapto-4-(2-pyrrolecarboxalideneamino)-s-triazole] in 1:1 and 1:2 molar ratios. All of the compounds were characterized by elemental analysis, molar conductance, and IR, UV, 1H-, 13C- and metal-NMR spectral studies. All the spectral data suggest an involvement with an azomethine nitrogen in coordination to the central metal atom. With the help of above-mentioned spectral studies, penta and hexacoordinated environments around the central metal atoms in the 1:1 and 1:2 complexes, respectively, are proposed. Finally, the free ligands and their metal complexes were tested in vitro against some pathogenic bacteria and fungi to assess their antimicrobial properties.

scholarly journals Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands

Inorganics ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 66
Author(s):  
Gurunath Sahu ◽  
Edward R. T. Tiekink ◽  
Rupam Dinda
Keyword(s):  
Schiff Base ◽  
Dna Interaction ◽  
Mouse Embryonic Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Crystallographic Analysis ◽  
Ligand Molecule ◽  
Cytotoxicity Activity ◽  
Pyramidal Geometry ◽  
Cytotoxicity Studies

Two new oxidovanadium(V) complexes, (HNEt3)[VVO2L] (1) and [(VVOL)2μ-O] (2), have been synthesized using a tridentate Schiff base ligand H2L [where H2L = 4-((E)-(2-hydroxy-5-nitrophenylimino)methyl)benzene-1,3-diol] and VO(acac)2 as starting metal precursor. The ligand and corresponding metal complexes are characterized by physicochemical (elemental analysis), spectroscopic (FT-IR, UV–Vis, and NMR), and spectrometric (ESI–MS) methods. X-ray crystallographic analysis indicates the anion in salt 1 features a distorted square-pyramidal geometry for the vanadium(V) center defined by imine-N, two phenoxide-O, and two oxido-O atoms. The interaction of the compounds with CT–DNA was studied through UV–Vis absorption titration and circular dichroism methods. The results indicated that complexes showed enhanced binding affinity towards DNA compared to the ligand molecule. Finally, the in vitro cytotoxicity studies of H2L, 1, and 2 were evaluated against colon cancer (HT-29) and mouse embryonic fibroblast (NIH-3T3) cell lines by MTT assay. The results demonstrated that the compounds manifested a cytotoxic potential comparable with clinically referred drugs and caused cell death by apoptosis.

Controlled Release of Metformin Hydrochloride I. In vitro Release from Physical Mixture Containing Xanthan Gum as Hydrophilic Rate Retarding Polymer

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mashkura Ashrafi ◽  
Jakir Ahmed Chowdhury ◽  
Md Selim Reza
Keyword(s):  
Controlled Release ◽  
Xanthan Gum ◽  
In Vitro Release ◽  
Correlation Coefficients ◽  
High Ratio ◽  
Water Soluble ◽  
Metformin Hydrochloride ◽  
Model Drug ◽  
Very High

Capsules of different formulations were prepared by using a hydrophilic polymer, xanthan gum and a filler Ludipress. Metformin hydrochloride, which is an anti-diabetic agent, was used as a model drug here with the aim to formulate sustained release capsules. In the first 6 formulations, metformin hydrochloride and xanthan gum were used in different ratio. Later, Ludipress was added to the formulations in a percentage of 8% to 41%. The total procedure was carried out by physical mixing of the ingredients and filling in capsule shells of size ‘1’. As metformin hydrochloride is a highly water soluble drug, the dissolution test was done in 250 ml distilled water in a thermal shaker (Memmert) with a shaking speed of 50 rpm at 370C &plusmn 0.50C for 6 hours. After the dissolution, the data were treated with different kinetic models. The results found from the graphs and data show that the formulations follow the Higuchian release pattern as they showed correlation coefficients greater than 0.99 and the sustaining effect of the formulations was very high when the xanthan gum was used in a very high ratio with the drug. It was also investigated that the Ludipress extended the sustaining effect of the formulation to some extent. But after a certain period, Ludipress did not show any significant effect as the pores made by the xanthan gum network were already blocked. It is found here that when the metformin hydrochloride and the xanthan gum ratio was 1:1, showed a high percentage of drug release, i.e. 91.80% of drug was released after 6 hours. But With a xanthan gum and metformin hydrochloride ratio of 6:1, a very slow release of the drug was obtained. Only 66.68% of the drug was released after 6 hours. The percent loading in this case was 14%. Again, when Ludipress was used in high ratio, it was found to retard the release rate more prominently. Key words: Metformin Hydrochloride, Xanthan Gum, Controlled release capsule Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 21 ◽  
Author(s):  
Muhammad Arfat Yameen ◽  
Amir Zeb ◽  
Raza E Mustafa ◽  
Sana Mushtaq ◽  
Nargis Aman ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Hybrid Material ◽  
Ag Nanoparticles ◽  
Hybrid Composite ◽  
Synthesis Method ◽  
Biological Evaluation ◽  
Vitro Assay ◽  
Cytotoxicity Studies ◽  
Material Composite

Background: Incoherent use of antibiotics has led toward resistance in MRSA, which is becoming multidrugresistant with high rate of virulence in the community and hospital settings. Objective: Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles and chitosan which were prepared by one-step synthesis method and various characterizations were performed. Methods: Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques while agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies was done by skin abrasion mouse model and MTT assay on RD cell respectively. Results: All isolates were resistant with the tested antibiotics except vancomycin. MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1. The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm respectively. Morphology of spheres was uneven, varied, porous and irregular in SEM and Ag nanoparticles presence and formation was also seen in micrograph. No substantial interface among drug, nanoparticles and polymer was found in XRD and IR showed characteristic peaks of all compound in the formulation. The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm). A significant reduction in microbial burden (~6.5 log10 CFU ml-1) was seen in vivo with loaded hybrid composite spheres formulation. The MTT assay indicated no potential cytotoxicity with hybrid composite spheres. Conclusion: Synergistic effect, amoxicillin, new hybrid formulation, anti-MRSA activity, composite spheres. nanoparticles.

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