scholarly journals The Possibility of Analyzing Endometrial Receptivity Using Cells From Embryo Transfer Catheters

2020 ◽  
Author(s):  
Kaori Goto ◽  
Yasushi Kawano ◽  
Takafumi Utsunomiya ◽  
Hisashi Narahara
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Protein Expression ◽  
Embryo Transfer ◽  
Gene Expression Profile ◽  
Estrogen Receptor Α ◽  
Endometrial Receptivity ◽  
Endometrial Cells ◽  
The Relationship

Abstract Background: Endometrial receptivity issues represent a potential source of implantaion failure. It is very important to investigate the expression of endometrial receptive markers in the endometrium during implantation. Therefore, we examined whether it would be possible to analyze endometrial receptivity using cells from embryo transfer catheters. In addition, we analyzed the relationship between the gene expression profile associated with pregnancy from endometrial cells taken during embryo transfer.Methods: A total of 88 cycles from 88 consenting patients were enrolled in this study. The tip of the embryo transfer (ET) catheter was cut and immersed in a dedicated reagent. Confirmation of cell distribution was carried out using a Papanicolaou stain (n=6) and immunocytochemistry (n=3). Protein expression was carried out by immunocytochemistry (n=12). Total RNA was extracted, and the expression of endometrial receptive markers (estrogen receptor α, progesterone receptor, and homeobox A10) were analyzed using quantitative reverse transcription polymerase chain reaction (n=67). we analyzed the relationship between the gene expression profile associated with pregnancy from endometrial cells. Results: Samples collected from the ET catheter showed clear staining for endometrial cells. Most of the cells were endometrial epithelial cells. Cervical cells were not included. The protein expression of endometrial receptive markers in cells was also confirmed. Three genes were analyzed that are associated with endometrial receptivity. Progesterone receptor expression was 1.5-fold and homeobox A10 expression was 2-fold higher in patients who became non-pregnant group, compared to the pregnant group. Both increases were statistically significant (p < 0.05). Estrogen receptor α expression tended to be higher in the non-pregnant group, but there was no significant difference. Conclusion: Our results suggest that endometrial receptivity can be evaluated using cells obtained from the ET catheter. This method may be useful for elucidating the cause of implantation failure by comparing a receptive and non-receptive endometrium at the time of ET.

G-protein Coupled Estrogen Receptor Expression in Growth Hormone Secreting and Non-Functioning Adenomas

2020 ◽  
Author(s):  
Hande Mefkure Ozkaya ◽  
Muge Sayitoglu ◽  
Nil Comunoglu ◽  
Eda Sun ◽  
Fatma Ela Keskin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
G Protein ◽  
Estrogen Receptor Α ◽  
Receptor Expression ◽  
Positive Correlation ◽  
Polymerase Chain ◽  
G Protein Coupled ◽  
Transforming Gene

Abstract Purpose To evaluate the expression of G-protein coupled estrogen receptor (GPER1), aromatase, estrogen receptor α (ERα), estrogen receptor β (ERβ), pituitary tumor transforming gene (PTTG), and fibroblast growth factor 2 (FGF2) in GH-secreting and non-functioning adenomas (NFA). Methods Thirty patients with acromegaly and 27 patients with NFA were included. Gene expression was determined via quantitative reverse transcription polymerase chain reaction (QRT-PCR). Protein expression was determined via immunohistochemistry. Results There was no difference, in terms of gene expression of aromatase, ERα, PTTG, and FGF2 between the two groups (p>0.05 for all). ERβ gene expression was higher and GPER1 gene expression was lower in GH-secreting adenomas than NFAs (p<0.05 for all). Aromatase and ERβ protein expression was higher in GH-secreting adenomas than NFAs (p=0.01). None of the tumors expressed ERα. GPER1 expression was detected in 62.2% of the GH-secreting adenomas and 45% of NFAs. There was no difference in terms of GPER1, PTTG, FGF2 H scores between the two groups (p>0.05 for all). GPER1 gene expression was positively correlated to ERα, ERβ, PTTG, and FGF2 gene expression (p<0.05 for all). There was a positive correlation between aromatase and GPER1 protein expression (r=0.31; p=0.04). Conclusions GPER1 is expressed at both gene and protein level in a substantial portion of GH-secreting adenomas and NFAs. The finding of a positive correlation between GPER1 and ERα, ERβ, PTTG, and FGF2 gene expression and aromatase and GPER1 protein expression suggests GPER1 along with aromatase and classical ERs might mediate the effects of estrogen through upregulation of PTTG and FGF2.

scholarly journals The Relationship Among HOXA10, Estrogen Receptor α, Progesterone Receptor, and Progesterone Receptor B Proteins in Rectosigmoid Endometriosis

2014 ◽  
Vol 22 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Alysson Zanatta ◽  
Ricardo Mendes Alves Pereira ◽  
André Monteiro da Rocha ◽  
Bruno Cogliati ◽  
Edmund Chada Baracat ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Estrogen Receptor Α ◽  
Progesterone Receptor B ◽  
The Relationship

scholarly journals 124 THE MAJOR GENE EXPRESSION PATTERNS IN ENDOMETRIAL TISSUE OF PIGS DURING EARLY GESTATION

2005 ◽  
Vol 17 (2) ◽  
pp. 212
Author(s):  
B.-K. Kim ◽  
H.J. Chung ◽  
Y.G. Ko ◽  
Y.M. Kim ◽  
H.-H. Seong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Expression Patterns ◽  
Estrogen Receptor Α ◽  
Endometrial Tissue ◽  
Estrogen Receptor Β ◽  
Uterine Receptivity ◽  
Early Gestation ◽  
Tgf Β1

Although the expression of important genes in the embryo at pre-implantation stage, which encompasses the period from fertilization to implantation, have been reported for mice and cows, little information relevant to this subject is known in pigs. The objective of this study was to investigate the changes of importantly expressed genes and proteins in endometrial tissue of pigs from fertilization to implantation. Six genes, including estrogen receptor-α, estrogen receptor-β, LIF, LR (LIF receptor), TGFβ1, and TGFβ2, that may play important roles in regulating uterine receptivity and successful implantation and that show different expression patterns by the stages of pregnancy were selected. As a step toward understanding the role of gene and protein expression changes in endometrial tissue of pigs during the preimplantation stage (Day 2, Day 6, Day 8, Day 12, and Day 17, n = 3/group) and the post-implantation stage (Day 21 and Day 33, n = 3/group) and Day 0 (estrous), real-time PCR methods for quantitative analysis of genes and immunohistochemistry methods to localize protein expression were utilized. Data from quantitative real-time PCR were analyzed by ANOVA. The results of this experiment indicated that estrogen receptor-β mRNA level was sharply increased to Day 12 of pregnancy, while estrogen receptor-α mRNA did not change drastically during early pregnancy stage. In contrast, levels of LIF and LR mRNA were increased from Day 2 to Day 33. Although TGFβ1 mRNA reached peak on Day 17 and TGFβ2 mRNA showed the highest level on Day 17, TGFβ2 did not appear to change drastically. For the protein expression patterns, estrogen receptor-α and estrogen receptor-β proteins were expressed in both luminal epithelium and glandular epithelium, but they were only partially expressed in some tissues of stroma cells. LIF protein was expressed in all cell types, while TGF β1 protein was high expressed in glandular epithelium. Also, ERs, LIF, and TGF β1 mRNA and protein expression showed stage- and cell-specific expression patterns. We also investigated the gene expression of TGF β1 mRNA and TGF β2 mRNA in early conceptus (Day 12 and Day 17). TGF β1 mRNA expression was low in Day 12 embryos, and increased progressively to Day 17. This indicated that both the maternal uterus and the conceptus represent the same gene expression pattern. These results suggest that estrogen receptor-β could be an important factor in estrogen action in endometrial tissue during early gestation in pigs, and TGF βs function in both autocrine and paracrine interactions. Progressive increase in TGF β1 mRNA expression in conceptus and uterine tissues suggest important roles of TGF β1 in conceptus development and establishment of the uterine receptivity during the peri-implantation period.

scholarly journals Loading-related regulation of gene expression in bone in the contexts of estrogen deficiency, lack of estrogen receptor α and disuse

Bone ◽  
2010 ◽  
Vol 46 (3) ◽  
pp. 628-642 ◽  
Author(s):  
Gul Zaman ◽  
Leanne K. Saxon ◽  
Andrew Sunters ◽  
Helen Hilton ◽  
Peter Underhill ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Regulation Of Gene Expression ◽  
Estrogen Receptor Α ◽  
Estrogen Deficiency

scholarly journals Clinical applications of endometrial receptivity tests in patients with recurrent implantation failure

2021 ◽  
Vol 16 (1) ◽  
pp. 79-85
Author(s):  
Ioan BOLEAC ◽  
◽  
Manuela NEAGU ◽  
Anca CORICOVAC ◽  
Dorina CODREANU ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Embryo Transfer ◽  
Expression Profile ◽  
Clinical Applications ◽  
Endometrial Receptivity ◽  
Clinical Pregnancy ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Endometrial Cells

Recurrent implantation failure is represented by the failure to achieve a clinical pregnancy after transfer of at least 4 good-quality embryos in a minimum of 3 fresh or frozen cycles in a woman under the age of 40 years. One of the recent approaches in studying the window of implantation was building the expression profile of the genes of the endometrial cells. We performed a retrospective study which investigated if endometrial receptivity tests improved the outcomes of IVF procedures in patients with recurrent implantation failure. We enrolled 47 couples with RIF and divided them in 2 groups: the first group of 22 couples performed the ERA test and the embryo transfer according to the result of the test; the second group of 27 couples had the embryo transfer done without the ERA test. Our conclusion was that the ERA test did not improve the outcomes for patients with recurrent implantation failure.

Sign in / Sign up

Export Citation Format

Share Document