scholarly journals The Importance of pH Adjustment for Preventing Fibrin Glue Dissolution in the Stomach: an in Vitro Study

2021 ◽  
Author(s):  
Yoshitaka Takegawa ◽  
Toshitatsu Takao ◽  
Hiroya Sakaguchi ◽  
Tatsuya Nakai ◽  
Kazuhiro Takeo ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Fibrin Glue ◽  
In Vitro Study ◽  
Preventive Measure ◽  
Polyglycolic Acid ◽  
Fibrin Clot ◽  
Dissolution Time ◽  
Fibrin Gel ◽  
Three Samples

Abstract Background and study aim: Combined use of fibrin glue and polyglycolic acid (PGA) sheets has attracted attention as a preventive measure for complications associated with endoscopic submucosal dissection. However, fibrin glue is a protein that may be dissolved by gastric acid. We evaluated the effect of artificial gastric acid on fibrin clot.Materials and methods: The dissolution time of three layers of fibrin glue with PGA sheets was measured in five groups (pH 1.2, 2.0, 4.0, 5.5, and 6.0 with pepsin). Measurements of three samples per group were made. The mean number of the remaining layers at each measurement point was observed for seven days.Results: The time to complete dissolution of the three layers of fibrin gel in the three samples was 150 minutes at pH 1.2, 5 hours at pH 2.0, 24 hours at pH 4.0, and 2 days and 6 hours at pH 5.5. Conclusion: In order to maintain fibrin glue in the stomach for a long period, there was a need to avoid pepsin activation secondary to acidification of gastric juice. The use of strong antacids is recommended.

Abstract TP35: Estimation of Red Blood Cells in the Thrombus Using MRI. A Phantom Study with Predetermined Thrombus Components

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Ana Paula Narata ◽  
Isabelle Filipiak ◽  
Richard Bibi ◽  
Jean Philippe Cottier ◽  
Kevin Janot
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Spin Echo ◽  
In Vitro Study ◽  
Fibrin Clot ◽  
Fluid Attenuated Inversion Recovery ◽  
Magnetic Resonance Imaging Mri ◽  
C 50 ◽  
D 20

Background and Purpose: Better understanding about thrombus composition seems necessary, as treatment of acute ischemic stroke (AIS) is focus on clot chemical dissolution and mechanical extraction. We propose to evaluate whether magnetic resonance imaging (MRI) can differentiate white from red clots and estimate red blood cells percentage (RBC%) using clots with predetermined components and an index based on MRI signal intensity (SI). Material and Methods: 5 clots (A=100% fibrin, B=80% RBC, C=50% RBC, D=20% RBC, E=unknown) were fixed in gelatin-manganese solution and studied by: high-resolution 3D T1-weighted (T1MPR), T2-weighted turbo spin echo (T2TSE), T2-weighted gradient echo (T2GE), susceptibility weighted (SWI), fluid-attenuated inversion recovery (FLAIR) and diffusion weighted imaging (DWI) with apparent diffusion coefficient (ADC). SI index was calculated with clot SI and gelatin SI. Statistical analysis compared RBC-clots to fibrin-clot SI index and the correlation of RBC% and SI index in each MRI sequence. Results: Each red clot was different from clot A except clot D in FLAIR. Correlation between clots SI index and RBC concentration were found in T1MPR (r=-0.84), SWI (r=-0.79), T2GE (r=-0.72) and FLAIR (r=0.80). Linear regression resolution provided an indirect RBC estimation for clot E: 47.3 % in T1MPR, SWI 41.5%, T2GE 45.1% and FLAIR 50.9%. Histological analysis confirmed clot E composition. Conclusion: This in vitro study suggests that MRI can differentiate white from red clots except clots with low RBC% in FLAIR and also provide approximate RBC%.

Comparative evaluation of surface roughness and texture of polished nanofill and nanohybrid composites using profilometer and scanning electron microscope: in - vitro study

2017 ◽  
Vol 9 (2) ◽  
pp. 4-10
Author(s):  
Krishna Prasada L ◽  
Jyothsna S Jathanna ◽  
Naveen Kumar ◽  
Ramya M.K ◽  
Elizabeth Issac
Keyword(s):  
Surface Roughness ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Resin Composite ◽  
In Vitro Study ◽  
Average Value ◽  
Three Samples ◽  
Group B ◽  
Scanning Electron

BACKGROUND: To determine the effectiveness of three polishing systems on three different composite materials by evaluating surface roughness using a Profilometer and Scanning electron microscope. METHODOLOGY: A total of Sixty-three resin composite disks were prepared in rectangular acrylic mould of 8×2mm dimension, with 4mm thickness. Specimens were made of light activated resin composite Filtek Z-250-XT, Tetric-N-Ceram bulkfill, Ceram X Duo. The sixty-three samples were divided into three groups of twenty-one samples each i.e. Group A (Filtek Z-250-XT), Group B (TetricN-Ceram bulkfill) and Group C (Ceram-X-Duo).Out of 21 samples of each of the material, 7 specimens were polished with multi enhance polishing agents, 7 specimens were polished with super snap polishing agents and 7 specimens were polished with sof-lexdiscs.Surface roughness of each sample after polishingwasevaluatedusingProfilometer and Scanning electron microscope. RESULTS: One-way anova and kruskalwallis test was used for statistical analysis. Ceram-X-Duo gave least roughness average value with Super snap polishing system when compared to multi enhance and sof-lex polishing system. CONCLUSION: Super snap polishing system is a better polishing system than multi enhance and soflex and Ceram-X-Duo composite material offers better polishability compared to Filtek Z-250-XT and Tetric-N-Ceram bulk fill.

Fibrin Glue in Skin Healing: An In Vitro Study

1994 ◽  
pp. 52-59
Author(s):  
M.-F. Harmand ◽  
D. Michel ◽  
M. Bagot d’Arc ◽  
P. Gonfrier
Keyword(s):  
Fibrin Glue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Skin Healing

Chondrocytes suspended in modified fibrin glue for vocal fold augmentation: An in vitro study in a porcine larynx model

Head & Neck ◽  
2011 ◽  
Vol 34 (5) ◽  
pp. 667-673 ◽  
Author(s):  
Daniel Skodacek ◽  
Ulrike Arnold ◽  
Katharina Storck ◽  
Carmelo Perez Alvarez ◽  
Sabine Ratzinger ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Vocal Fold ◽  
In Vitro Study ◽  
Vitro Study ◽  
Vocal Fold Augmentation

scholarly journals Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Mari Matoba ◽  
Ayumi Hashimoto ◽  
Ayumi Tanzawa ◽  
Taichi Orikasa ◽  
Junki Ikeda ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Standard Treatment ◽  
Polyglycolic Acid ◽  
Fibroblast Growth ◽  
Peritoneal Adhesions ◽  
Microscopic Evaluation ◽  
Well Differentiated ◽  
Rat Experiments ◽  
Hematoxylin Eosin

Postoperative intra-abdominal or intrathoracic adhesions sometimes cause significant morbidity. We have designed three types of alginate-based treatments using strongly cross-linked (SL), weakly cross-linked (WL), and non-cross-linked (NL) alginate with calcium gluconate. In rat experiments, we compared the antiadhesive effects of the three types of alginate-based treatments, fibrin glue treatment (a standard treatment), and no treatment against adhesions caused by polyglycolic acid (PGA) mesh (PGA-induced adhesions). The antiadhesive materials were set on the PGA sheet fixed on the parietal peritoneum of the abdomen. Fifty-six days later, the adhesions were evaluated macroscopically by the adhesion scores and microscopically by hematoxylin-eosin staining and immunostaining. We also tested the fibroblast growth on the surface of the antiadhesive materialsin vitro. The antiadhesive effects of WL and NL were superior to the no treatment and fibrin glue treatment. A microscopic evaluation confirmed that the PGA sheet was covered by a peritoneal layer constructed of well-differentiated mesothelial cells, and the inflammation was most improved in the NL and WL. The fibroblast growth was inhibited most on the surfaces of the NL and WL. These results suggest that either the WL or NL treatments are suitable for preventing PGA-induced adhesions compared to SL or the conventional treatment.

Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix

1997 ◽  
Vol 110 (7) ◽  
pp. 861-870 ◽  
Author(s):  
D. Greiling ◽  
R.A. Clark
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Collagen Gel ◽  
Fibrin Clot ◽  
Dependent Manner ◽  
Fibrin Gel ◽  
Concentration Dependent Manner ◽  
Vitro Model ◽  
Early Wound

After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into the fibrin clot and contribute to the formation of granulation tissue. The functional mechanisms allowing fibroblasts to leave the collagenous matrix of normal connective tissue and invade the provisional matrix of the fibrin clot have not been fully defined. To investigate these mechanisms we established a new in vitro model which simulates specific aspects of early wound healing, that is, the migration of fibroblasts from a three-dimensional collagen matrix into a fibrin clot. This transmigration could be induced by physiological concentrations of platelet releasate or platelet-derived growth factor BB (PDGF-BB) in a concentration-dependent manner. At 24 hours irradiated fibroblasts invaded the fibrin gel almost as well as non-irradiated cells, indicating that transmigration was independent of proliferation. Plasminogen and its activators appear to be necessary for invasion of the fibrin clot since protease inhibitors decreased the amount of migration. These serine proteases, however, were not necessary for exit from the collagen gel as fibroblasts migrated out of the collagen gel onto a surface coated with fibrin fibrils even in the presence of inhibitors. Removal of fibronectin (FN) from either the collagen gel or the fibrin gel markedly decreased the number of migrating cells, suggesting that FN provides a conduit for transmigration. Cell movement in the in vitro model was inhibited by RGD peptide, and by monoclonal antibodies against the subunits of the alpha5 beta1 and alpha v beta3 integrin receptor. Thus, the functional requirements for fibroblast transmigration from collagen-rich to fibrin-rich matrices, such as occurs in early wound healing, have been partially defined using an in vitro paradigm of this important biologic process.

scholarly journals Thermal Conductivity of Different Colored Compomers

2017 ◽  
Vol 15 (4) ◽  
pp. e362-e368 ◽  
Author(s):  
Cigdem Guler ◽  
Ali Keles ◽  
Mehmet S. Guler ◽  
Sendogan Karagoz ◽  
Ömer N. Cora ◽  
...  
Keyword(s):  
Thermal Conductivity ◽  
Heat Conductivity ◽  
Material Properties ◽  
In Vitro Study ◽  
Test Setup ◽  
Three Samples ◽  
Uncertainty Analyses ◽  
Coefficient Of Heat Conductivity ◽  
Deep Cavities

Background Compomers are mostly used in primary dentition. The thermal conductivity properties of traditional or colored compomers have not been investigated in detail so far. The aim of this in vitro study was to assess and compare the thermal conductivities of traditional and colored compomers. Method Two sets of compomers – namely, Twinky Star (available in berry, lemon, green, silver, blue, pink, gold and orange shades) and Dyract Extra (available in B1, A3 and A2 shades) –were included in this study. All of the traditional and colored compomers were applied to standard molds and polymerized according to the manufacturers’ instructions. Three samples were prepared from each compomer. Measurements were conducted using a heat conduction test setup, and the coefficient of heat conductivity was calculated for each material. The heat conductivity coefficients were statistically analyzed using Kruskal-Wallis and Duncan tests. Uncertainty analysis was also performed on the calculated coefficients of heat conductivity. Results Statistically significant differences were found (p<0.05) between the thermal conductivity properties of the traditional and colored compomers examined. Among all of the tested compomers, the silver shade compomer exhibited the highest coefficient of heat conductivity (p<0.05), while the berry shade exhibited the lowest coefficient (p<0.05). Uncertainty analyses revealed that 6 out of 11 samples showed significant differences. Conclusions The silver shade compomer should be avoided in deep cavities. The material properties could be improved for colored compomers.

In vivo and in vitro study of suprachoroidal fibrin glue

2009 ◽  
Vol 53 (6) ◽  
pp. 640-647 ◽  
Author(s):  
Jing Hou ◽  
Yong Tao ◽  
Yan-rong Jiang ◽  
Kai Wang
Keyword(s):  
Fibrin Glue ◽  
In Vitro Study ◽  
Vitro Study

The effect of diode laser irradiation on dentin as a preventive measure against dental erosion: an in vitro study

2010 ◽  
Vol 26 (5) ◽  
pp. 615-621 ◽  
Author(s):  
Mary A. S. de-Melo ◽  
Vanara F. Passos ◽  
Jose J. Alves ◽  
Eduardo B. Barros ◽  
Sérgio L. Santiago ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
In Vitro Study ◽  
Preventive Measure ◽  
Dental Erosion ◽  
Vitro Study

scholarly journals Biocompatibility of electrospinning polycaprolactone, polylactic acid, their blends and copolymers scaffolds in in vitro tests if mesenchyme stem cells

2021 ◽  
Vol 8 (5) ◽  
pp. 38-49
Author(s):  
A. I. Mishanin ◽  
A. N. Panina ◽  
E. N. Bolbasov ◽  
S. I. Tverdokhlebov ◽  
A. S. Golovkin
Keyword(s):  
Stem Cells ◽  
Polylactic Acid ◽  
Biodegradable Polymers ◽  
In Vitro Study ◽  
Polyglycolic Acid ◽  
In Vitro Tests ◽  
Adhesion Properties ◽  
Acid Copolymer ◽  
Mesenchyme Stem Cells

Background. Biodegradable polymers are one of the most promising groups of materials suitable for creating tissue-engineered scaffolds. The high interest in biopolymers is associated with the possibility of creating scaffolds with desired properties, through the use of mixtures and copolymers. The determination of the key parameters of biocompatibility is the basic purpose for testing created materials.Objective. To perform the comparative in vitro study of biocompatibility properties of biopolymer scaffolds produced using polycaprolactone, polylactic acid, their mixtures and copolymers by electrospinning technology.Design and methods. The adhesion properties and cytotoxicity of scaffolds made from polycaprolactone, polylactic acid, copolymer of L- and D-isoforms of lactic acid, their mixtures and co-polymers with the addition of polyglycolic acid were investigated after scaffolds co-cultivation with human mesenchyme stem cells (MSC).Results. The largest number of spread spindle-shaped MSCs was on the surface of polymers containing polyglycolic acid. Besides, the cells on the surface of the copolymer with polyglycolic acid had the morphology closest to the control. The lowest number of living cells was found on the surface of polylactic acid scaffolds, and the highest on the surface of samples from of polycaprolactone and polylactic acid blend.Conclusion. Thus, all tested polymers had good adhesion properties in experiments with human mesenchyme stem cells were possessed by biodegradable polymers with the addition of polyglycolic acid. 

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