Development of an In Vitro Model Assay System for the Evaluation of the Effects of Toxic Chemicals on Human Airways

1994 ◽  
Author(s):  
James L. Ellis ◽  
Margaret G. Filbert
Keyword(s):  
In Vitro Model ◽  
Assay System ◽  
Toxic Chemicals ◽  
Vitro Model ◽  
Human Airways

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Präsidentenposter: Regulation der Expression der β-Defensine hBD-2 und hBD-3 in einem in vitro Model der Candida Ösophagitis: NF-κB, MAPK/AP-1 und EGFR

2009 ◽  
Vol 47 (09) ◽  
Author(s):  
N Steubesand ◽  
K Kiehne ◽  
R Pahl ◽  
G Brunke ◽  
UR Fölsch ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Vitro Model

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model

A novel in vitro model for joint effects of alcohol and free fatty acids on hepatocellular steatosis and inflammation

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
A Mahli ◽  
WE Thasler ◽  
M Müller ◽  
C Hellerbrand
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
In Vitro Model ◽  
Joint Effects ◽  
Vitro Model

Human 3-dimensional liver organoids: in vitro model systems to study liver diseases

2020 ◽  
Author(s):  
H Gaitantzi ◽  
C Cai ◽  
S Asawa ◽  
K Böttcher ◽  
M Ebert ◽  
...  
Keyword(s):  
Liver Diseases ◽  
In Vitro Model ◽  
Model Systems ◽  
3 Dimensional ◽  
Vitro Model ◽  
Liver Organoids

Immunophenotyping of bone marrow-derived mesenchymal stem cells after transplantation in a heterologous in-vitro model using Laser Scanning Cytometry

2004 ◽  
Vol 52 (S 1) ◽  
Author(s):  
J Garbade ◽  
D Lenz ◽  
A Rastan ◽  
MJ Barten ◽  
A Tarnok ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Laser Scanning ◽  
In Vitro Model ◽  
Laser Scanning Cytometry ◽  
Vitro Model
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