scholarly journals 576 PB 053 METHODS TO MAXIMIZE AND MINIMIZE SEGREGATION OF CHIMERAL PEAR (PYRUS SPP) IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514c-514
Author(s):  
Abdelrahman S. Al-Wasel ◽  
Robert M. Skirvis
Keyword(s):  
Growth Regulators ◽  
Periclinal Chimera ◽  
Good Medium ◽  
Medium Selection

Variegated `Louise Bonne' (LB) pear is a periclinal chimera in which the LIII layer is albino. Chimeral shoots propagated in vitro segregate spontaneously into green, albino, pale, or rearranged chimeral types, making them difficult to maintain in culture. We investigated the role of growth regulators on chimeral stability and destability to find a combination that would maintain the chimera through repeated subcultures. 70 to 90% of shoots remained chimeral on Lepoivre (LP) medium supplemented with 8 μM BA or less. Only 36 to 58% of shoots grown at concentrations greater than 8 μM were stable. Shoots grown on LP with thidiazuron (TDZ) were very unstable (4 to 44%). NAA had no significant effect on chimeral stability. While shoots multiplied better on LP, the chimeral pattern was more obvious on MS, making it a good screening medium. Selection and subculturing chimeral shoots on a good medium (LP with 2 to 4 μM BA) increased the percentage of chimeral shoots from 26% at the 4th subculture to 84% at the 27th subculture.

scholarly journals In vitro propagation of Althaea officinalis : the role of plant growth regulators in morphogenesis

2017 ◽  
Vol 98 (3) ◽  
pp. 167-173
Author(s):  
A. Mujib ◽  
Tanu Pipal ◽  
Muzamil Ali ◽  
Dipti Tonk ◽  
Nadia Zafar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Althaea Officinalis

scholarly journals Role of plant growth regulators in preservation of Pyrus germplasm in vitro

2011 ◽  
Vol 10 (64) ◽  
pp. 14029-14037
Author(s):  
Ahmed Maqsood ◽  
Akbar Anjum Muhammad ◽  
Jamil Ahmed Mohammad ◽  
Mustafa Sajid Ghulam ◽  
Yoqub Abid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators

scholarly journals In vitro neoformation of grape chimeras (Vitis vinifera L.)

OENO One ◽  
1999 ◽  
Vol 33 (1) ◽  
pp. 1 ◽  
Author(s):  
Sandrine Verdisson ◽  
Fabienne Baillieul ◽  
Jean-Claude Audran
Keyword(s):  
Plant Growth ◽  
Vitis Vinifera ◽  
Growth Regulators ◽  
Rapd Analysis ◽  
Adventitious Shoots ◽  
Pinot Noir ◽  
Periclinal Chimera ◽  
Dark Regime ◽  
First Time

<p style="text-align: justify;">Difference in grape sensitivity to <em>Botrytis cinerea</em> attacks between cultivars was explained by differences in the epidermic tissue of the fruit. Therefore, this work was conducted to create a grape periclinal chimera, whose fruits would combine the skin of a <em>Botrytis</em> tolerant cultivar with a pulp of an another cultivar admitted its good organoleptic quality and productivity. In a first time, graftings of two cultivars (Chardonnay and Pinot noir) were conducted <em>in vitro</em> on 5 different media supplemented with various plant growth regulators. Adventitious shoots were only observed on medium containing BAP and GA3 from a mixed callus structure after four weeks of darkness followed by a light/dark regime. In a second time, RAPD analysis, conducted on these plants, showed their chimerical characteristics.</p>

scholarly journals Regulation of in vitro somatic embryogenesis with emphasis on to the role of endogenous hormones

2001 ◽  
Vol 13 (2) ◽  
pp. 196-223 ◽  
Author(s):  
VÍCTOR M. JIMÉNEZ
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Growth Regulators ◽  
Culture Medium ◽  
Endogenous Hormones ◽  
Tissue Cultures ◽  
Factors Associated ◽  
General Aspects

Different aspects of the in vitro somatic embryogenesis regulation are reviewed in this paper.work. A description of g General aspects, such as terminology, uses, stages of development and factors associated with the somatic embryogenesis, are described. is carried out. Although a brief description ofn the effects of the addition of different plant growth regulators to the culture medium wasis given, the article is centereds itself on the effect that the endogenous hormone concentrations in the initial explants and in the tissue cultures derived from them could play oin the induction and expression of somatic embryogenesis. It is significant that few to emphasize the low amount of systematic studies have been conducted, in this subject, in which different species and hormone groups were compared in cultures with and without embryogenic capacity. Moreover, the lack of correlation between the results presented in different studies the distinct works indicates that the hormone content of the cultures is not the only factor involved.

scholarly journals Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

2021 ◽  
Author(s):  
Antonio Calabuig-Serna ◽  
Carolina Camacho-Fernández ◽  
Ricardo Mir ◽  
Rosa Porcel ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Growth Regulators ◽  
Solanum Melongena ◽  
Microspore Embryogenesis ◽  
Anther Wall ◽  
Naphthaleneacetic Acid ◽  
Shed Light ◽  
Embryogenic Response

AbstractIn eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. In this work we aimed to shed light on the factors involved in the successful in vitro development of eggplant haploid embryos by evaluating the role of growth regulators (GRs) in this process. We analyzed the endogenous levels of different GRs, including auxins, cytokinins and gibberelins, as well as salicylic, jasmonic and abscisic acid, in microspores and microspore-derived embryos at different culture stages. We also analyzed the same GR profiles in leaf and anther wall tissues of different eggplant backgrounds. Finally, we assessed the application of different GR combinations to the culture medium. Our results showed that in eggplant there are no genotype-specific endogenous GR profiles that can be associated to a high embryogenic response. Instead, the embryogenic response seems related to different GR accumulation patterns during in vitro culture. The changes observed in the endogenous levels of salicylic and abscisic acid were not related to the embryo transition. There were, however, changes in the levels of indole acetic acid and dihydrozeatin. The best GR combination to promote callus production was 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.5 mg/L 6-benzylaminopurine (BAP). A 20% reduction of NAA and BAP reduced embryo production but produced structures more anatomically similar to embryos. These results shed light on the role of GRs during the development of microspore-derived embryos in eggplant microspore cultures.

scholarly journals Apprehending the potential of BABY BOOM transcription factors to mitigate cotton regeneration and transformation

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Caner YAVUZ ◽  
Shakhnozakhan TILLABOEVA ◽  
Allah BAKHSH
Keyword(s):  
Somatic Embryogenesis ◽  
Transcription Factors ◽  
Growth Regulators ◽  
Baby Boom ◽  
In Vitro Regeneration ◽  
Signal Transduction Pathway ◽  
Breeding Programmes ◽  
Transformation Problems

Abstract Since the advent of transgenic technology, the incorporation of gene(s) encoding traits of economic importance in cotton is being practiced worldwide. However, factors like recalcitrant nature of cotton cultivars, in vitro regeneration via tissue culture (especially via somatic embryogenesis), genotype dependency, long and toilsome protocols impede the pace of development of transgenic cotton. Besides that, types and age of explants, media composition, plant growth regulators and other environmental factors affect in vitro cotton regeneration significantly. The studies of genetic control of in vitro regeneration in plants have elucidated the role of certain transcription factor genes that are induced and expressed during somatic embryogenesis. Among these transcription factors, BABY BOOM (BBM) plays a very important role in signal transduction pathway, leading to cell differentiation and somatic embryos formation. The role of BBM has been established in plant cell proliferation, growth and development even without exogenous growth regulators. This review intends to provide an informative summary of regeneration and transformation problems in cotton and the latest developments in utilization of BBM transcription factors in cotton. We believe that the use of BBM will not only ease cotton genetic improvement but will also accelerate cotton breeding programmes.

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