scholarly journals Early Season Softwood Cuttings Effective for Vegetative Propagation of Juglans cinerea

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 697-700 ◽  
Author(s):  
Paula M. Pijut ◽  
Melanie J. Moore

Juglans cinerea L. (butternut) is a hardwood species valued for its wood and edible nuts. Information on the vegetative propagation of this species is currently unavailable. Our objective was to determine the conditions necessary for successful stem-cutting propagation of butternut. In 1999 and 2000, 10 trees (each year) were randomly selected from a 5- and 6-year-old butternut plantation located in Rosemount, Minn. Hardwood stem cuttings were collected in March, April, and May. Softwood cuttings were collected in June and July. K-IBA at 0, 29, or 62 mm in water and IBA at 0, 34, or 74 mm in 70% ethanol were tested for root induction on cuttings. The basal end of cuttings were dipped in a treatment solution for 10 to 15 seconds, potted in a peat: perlite mixture, and placed in a mist bed for 5 to 8 weeks. Rooted cuttings were gradually hardened off from the mist bed, allowed to initiate new growth, over-wintered in a controlled cold-storage environment, and then outplanted to the field. For hardwood cuttings, rooting was greatest for those taken in mid-May (branches flushed out), 22% with 62 mm K-IBA and 28% with 74 mm IBA. Softwood cuttings rooted best when taken in June (current season's first flush of new growth or softwood growth 40 cm or greater) and treated with 62 mm K-IBA (77%) or 74 mm IBA (88%). For 1999, 31 out of 51 rooted softwood cuttings (60.8%) survived overwintering in cold storage and acclimatization to the field. For 2000, 173 out of 186 rooted softwood cuttings (93%) survived overwintering and acclimatization to the field. Chemical names used: indole-3-butyric acid-potassium salt (K-IBA); indole-3-butyric acid (IBA).

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 458F-459
Author(s):  
Paula M. Pijut ◽  
Melanie J. Barker

Butternut trees are becoming endangered as a result of butternut canker disease; thus, it is desirable to propagate disease-resistant trees for screening and provenance tests. The objective of this study was to determine the conditions necessary for successful cutting propagation. In 1998, 10 trees were selected from a 4-year-old butternut plantation located in Rosemount, Minn. Hardwood cuttings were collected 30 Mar., 21 Apr., and 6 May. The auxins, indole-3-butyric acid-potassium salt (KIBA) in water at 0, 29, or 74 mmol·L-1 were tested for root induction. The basal end of cuttings were dipped in treatment solutions for 10 to 15 s and placed in a 1 peat: 1 perlite mixture in Deepots™ (D40) in a mist bed. Mist was applied for 5 s every 15 min. Greenhouse conditions were: 12-h photoperiod provided by high-pressure sodium lamps (60 μmol·m-2·s-1), 22 °C, and bottom heat of 27 °C (heating pads). Softwood cuttings were collected 20 May, 18 June, 30 June, and 23 July. Rooting treatment solutions and greenhouse conditions were the same as for the hardwood cuttings, except no heating pads were used. Rooted cuttings were planted in Treepots™ (10 × 10 × 36 cm) and gradually hardened off from the mist bed. Hardwood cuttings from the first two collection dates did not initiate roots. Best rooting (12.5%) was achieved on hardwood cuttings collected 6 May using 29 mmol·L-1 KIBA. Softwood cuttings rooted to some degree at all concentrations of rooting solution and at every collection date. The greatest rooting (70%) was achieved using 74 mmol·L-1 IBA. In general, best rooting percentages were achieved with softwood cuttings collected 18 June and 23 July and treated with 62 mmol·L-1 KIBA or 74 mmol·L-1 IBA. Both rooted hardwood and softwood cuttings were successfully acclimatized from the mist bed and many have initiated new growth.


2018 ◽  
Vol 28 (3) ◽  
pp. 378-381
Author(s):  
Bryan J. Peterson ◽  
Stephanie E. Burnett ◽  
Olivia Sanchez

Although overhead mist revolutionized the propagation industry, it does suffer from potential drawbacks that include the application of large volumes of water, potentially unsanitary conditions, irregular misting coverage, and leaching of foliar nutrients. We explored the feasibility of submist as an alternative as it might avoid these problems by applying water exclusively from below the cutting, which is inserted basally into an enclosed rooting chamber. We propagated cuttings of korean lilac (Syringa pubescens ssp. patula) and inkberry (Ilex glabra) using both overhead mist and submist to compare effectiveness of the systems. Cuttings of korean lilac were wounded and dipped basally into 8000 mg·L−1 of the potassium salt of indole-3-butyric acid (K-IBA), and those in the overhead mist systems were inserted into coarse perlite. Cuttings of inkberry were wounded and treated with 5000 mg·L−1 K-IBA, and those in the overhead mist systems were inserted into 50:50 peat:perlite (by vol). Cuttings of korean lilac in the submist systems produced more than twice as many roots as cuttings in the overhead mist systems, with roots more than 2.6 times the length. Similarly, cuttings of inkberry in the submist systems produced more than three times the root counts and root lengths as cuttings in the overhead mist systems. For korean lilac, root dry weights averaged 58 mg for cuttings in the submist system, compared with only 18 mg among cuttings receiving overhead mist. Likewise, root dry weights averaged 70 and 7 mg for cuttings of inkberry propagated by submist and overhead mist, respectively. Rooted cuttings of korean lilac transplanted well into a soilless substrate, where they more than tripled their root biomass to 218 mg (vs. 59 mg for cuttings transplanted from overhead mist). We did not evaluate transplant performance of inkberry. Our results show that submist systems might merit consideration for the propagation of woody plants by leafy stem cuttings.


2014 ◽  
Vol 1 (2) ◽  
pp. 79-84
Author(s):  
Fadwa Mohammed Ahmed Ali ◽  
Yahia Hamid Ali Elbasheer

This paper describes a successful protocol of vegetative propagation by using stem cuttings of mature Peltophorum petrocarpum plants. The experiment was conducted in the nursery of the Forestry Research Centre at Soba during November 2011and April 2012. The socioeconomic importance of P. petrocarpum comes from its multipurpose uses and there is a demand in production of its seed for tree planting and domestication activities. Hence there is a need for efficient and economically feasible propagation method of P. pterocarpum. Therefore the objective of study is to develop a vegetative propagation protocol by using rooted stem cuttings; and to determine the effects of the rooting hormone indole-3-butyric acid (IBA) on rooting potentiality of stem cuttings of mature mother plants. The result revealed a significant effect (p<0.001) of IBA concentrations on rooting percentage and growth performance of stem cuttings. The highest rooting percent was obtained with 200ppm IBA which was not significantly different from 100ppm, but significantly different from 50 and 0.0ppm. The effect of IBA concentrations on root lengths and of roots per cutting was significantly different from 0.0 and 50ppm treatments. Although 100ppm IBA gave the tallest root (4.6 cm) but it was not significantly different from 200ppm (4.3 cm). Survival of rooted cuttings after transplanting from the propagator was 100% and 97% with 100 and 200mg IBA respectively. Accordingly the conclusion reached is that rooted cuttings can be used successfully for obtaining health, uniform plantations of P. petrocarpum for large-scale, biomass production and tree planting programmes.   


2020 ◽  
Vol 30 (1) ◽  
pp. 38-46
Author(s):  
Bryan J. Peterson ◽  
Gregory J.R. Melcher ◽  
Ailish K. Scott ◽  
Rebecca A. Tkacs ◽  
Andrew J. Chase

Sweetgale (Myrica gale), rhodora (Rhododendron canadense), and catberry (Ilex mucronata) are shrubs of eastern North America that may have potential for broader use in horticultural landscapes. Because information on their vegetative propagation is scarce, we conducted experiments over 2 years to evaluate the effects of cutting collection date, wounding, substrate composition, and the concentration of applied potassium salt of indole-3-butyric acid (K-IBA) on rooting of each species. In 2015, we collected cuttings of each species on three dates to obtain both softwood and semihardwood cuttings. Cuttings were unwounded or wounded with a razor blade, and treated by dipping into water containing K-IBA at concentrations ranging from 0 to 15,000 mg·L−1, after which they were inserted into a substrate of 3:1 perlite:peat (by volume) and placed under intermittent mist. In 2016, semihardwood cuttings of each species were all wounded, treated with K-IBA from 0 to 15,000 mg·L−1, and inserted into substrates of 100%, 75%, or 50% perlite, with the remaining volume occupied by peat. In both years, the greatest percentage of sweetgale cuttings rooted when no K-IBA was applied. K-IBA application also reduced root ratings, root dry weights, and root lengths of sweetgale. For rhodora and catberry, maximal responses for all measures of rooting occurred when 5000 to 15,000 mg·L−1 K-IBA was applied. We recommend that growers use no exogenous auxin to propagate sweetgale, and 5000 to 10,000 mg·L−1 K-IBA to propagate rhodora and catberry. Cuttings of all three species can be collected from softwood or semihardwood shoots. Finally, sweetgale can be rooted in perlite alone, whereas rhodora and catberry required the addition of peatmoss for satisfactory root development.


2000 ◽  
Vol 18 (4) ◽  
pp. 185-187
Author(s):  
Jeffrey G. Norcini ◽  
James H. Aldrich

Abstract Helianthus debilis Nutt. ssp. debilis ‘Flora Sun’, ‘Flora Sun’ beach sunflower, is a native, herbaceous perennial that should be propagated by cuttings to maintain its genetic and phenotypic fidelity. Stem cuttings were treated with a quick dip of a potassium salt of 1H-indole-3-butyric acid (K-IBA) solution at 0, 250, 500, 1000, 1500, 2000, 2500, or 3000 mg/liter (ppm) and rooted under intermittent mist. The root systems of six of twelve cuttings were harvested 17 and 21 days after treatment. The remaining six cuttings were transplanted in a soilless substrate to determine the effects of K-IBA on establishment in containers. Treatment of stem cuttings with K-IBA increased the number of roots and root fresh weight when cuttings were rooted under summer greenhouse conditions, but K-IBA did not provide any substantial short-term benefits in survival or growth of rooted cuttings after they were transplanted into plastic containers.


2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.


2011 ◽  
Vol 22 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Tarit Kumar Baul ◽  
Mohammad Mosharraf Hossain ◽  
Mohammad Mezbahuddin ◽  
Mohammed Mohiuddin

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.


2010 ◽  
Vol 28 (2) ◽  
pp. 69-73
Author(s):  
Jeff R. Jones ◽  
Anthony V. LeBude ◽  
Thomas G. Ranney

Abstract Deciduous azaleas (Rhododendron L.) offer a range of desirable ornamental characteristics and can be valuable nursery crops. Availability in the nursery trade, however, can be limited by the lack of effective propagation protocols. Therefore, the objectives of this research were to develop and optimize vegetative propagation protocols for Oconee azalea, Rhododendron flammeum (Michx.) Sarg., utilizing stem cuttings or in-field mound layering. An optimal method for producing rooted stem cuttings with large root systems (&gt; 20 cm2) was to collect softwood stem cuttings from hedged stock plants followed by treatment with 10,000 ppm of the potassium salt (K-salt) of indolebutyric acid (K-IBA). Mound layering was also effective. Fifty percent of the stems on each mound resulted in rooted layers and approximately six layers were produced per mound regardless of mounding season (March or June), with or without wounding, or application of 5000 ppm K-IBA to stems prior to mounding in June. Both softwood cuttings and mound layering can be utilized to produce high quality plants.


Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1151
Author(s):  
Ricardo A. Lesmes-Vesga ◽  
José X. Chaparro ◽  
Ali Sarkhosh ◽  
Mark A. Ritenour ◽  
Liliana M. Cano ◽  
...  

Traditionally, peach rootstocks are propagated by seeds due to their high availability, low cost, and easy storage and handling. However, stem cuttings allow the propagation of interspecific hybrids and keep the genetic uniformity of heterozygous genotypes. This study compared the effect of four different concentrations of K-IBA (indole-3-butyric acid potassium salt) on softwood cuttings of three peach backcrosses (peach × (peach × almond)) for rootstock propagation in two propagation systems: aeroponics and germination trays. The four concentrations of K-IBA applied were: 0.0% (w/v) as a control, 0.1% (w/v), 0.2% (w/v), and 0.4% (w/v). Data were collected on the survival rate (%), rooting rate (%), and root growth parameters. The relevance of auxin for peach cuttings rooting was evidenced. K-IBA at 0.2% showed the best rooting effect for peach softwood cuttings, evidenced by its high rooting rate and higher survival rate. K-IBA at 0.4% and 0.2% produced the highest number of adventitious roots. The highest root growth parameters were obtained in germination trays, confirming the suitability of this system for root growth. However, aeroponics was demonstrated to be as efficient as the traditional germination trays for the rooting of peach cuttings, allowing for a more controlled environment with a better use of resources.


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