scholarly journals Submist is Effective for Propagation of Korean Lilac and Inkberry by Stem Cuttings

2018 ◽  
Vol 28 (3) ◽  
pp. 378-381
Author(s):  
Bryan J. Peterson ◽  
Stephanie E. Burnett ◽  
Olivia Sanchez
Keyword(s):  
Butyric Acid ◽  
Woody Plants ◽  
Root Biomass ◽  
Potassium Salt ◽  
Foliar Nutrients ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Soilless Substrate

Although overhead mist revolutionized the propagation industry, it does suffer from potential drawbacks that include the application of large volumes of water, potentially unsanitary conditions, irregular misting coverage, and leaching of foliar nutrients. We explored the feasibility of submist as an alternative as it might avoid these problems by applying water exclusively from below the cutting, which is inserted basally into an enclosed rooting chamber. We propagated cuttings of korean lilac (Syringa pubescens ssp. patula) and inkberry (Ilex glabra) using both overhead mist and submist to compare effectiveness of the systems. Cuttings of korean lilac were wounded and dipped basally into 8000 mg·L−1 of the potassium salt of indole-3-butyric acid (K-IBA), and those in the overhead mist systems were inserted into coarse perlite. Cuttings of inkberry were wounded and treated with 5000 mg·L−1 K-IBA, and those in the overhead mist systems were inserted into 50:50 peat:perlite (by vol). Cuttings of korean lilac in the submist systems produced more than twice as many roots as cuttings in the overhead mist systems, with roots more than 2.6 times the length. Similarly, cuttings of inkberry in the submist systems produced more than three times the root counts and root lengths as cuttings in the overhead mist systems. For korean lilac, root dry weights averaged 58 mg for cuttings in the submist system, compared with only 18 mg among cuttings receiving overhead mist. Likewise, root dry weights averaged 70 and 7 mg for cuttings of inkberry propagated by submist and overhead mist, respectively. Rooted cuttings of korean lilac transplanted well into a soilless substrate, where they more than tripled their root biomass to 218 mg (vs. 59 mg for cuttings transplanted from overhead mist). We did not evaluate transplant performance of inkberry. Our results show that submist systems might merit consideration for the propagation of woody plants by leafy stem cuttings.

scholarly journals Cutting Propagation and Container Production of ‘Flora Sun’ Beach Sunflower

2000 ◽  
Vol 18 (4) ◽  
pp. 185-187
Author(s):  
Jeffrey G. Norcini ◽  
James H. Aldrich
Keyword(s):  
Butyric Acid ◽  
Potassium Salt ◽  
Root Systems ◽  
Stem Cuttings ◽  
Fresh Weight ◽  
Short Term ◽  
Rooted Cuttings ◽  
Soilless Substrate ◽  
Plastic Containers ◽  
Herbaceous Perennial

Abstract Helianthus debilis Nutt. ssp. debilis ‘Flora Sun’, ‘Flora Sun’ beach sunflower, is a native, herbaceous perennial that should be propagated by cuttings to maintain its genetic and phenotypic fidelity. Stem cuttings were treated with a quick dip of a potassium salt of 1H-indole-3-butyric acid (K-IBA) solution at 0, 250, 500, 1000, 1500, 2000, 2500, or 3000 mg/liter (ppm) and rooted under intermittent mist. The root systems of six of twelve cuttings were harvested 17 and 21 days after treatment. The remaining six cuttings were transplanted in a soilless substrate to determine the effects of K-IBA on establishment in containers. Treatment of stem cuttings with K-IBA increased the number of roots and root fresh weight when cuttings were rooted under summer greenhouse conditions, but K-IBA did not provide any substantial short-term benefits in survival or growth of rooted cuttings after they were transplanted into plastic containers.

scholarly journals Early Season Softwood Cuttings Effective for Vegetative Propagation of Juglans cinerea

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 697-700 ◽  
Author(s):  
Paula M. Pijut ◽  
Melanie J. Moore
Keyword(s):  
Butyric Acid ◽  
Cold Storage ◽  
Vegetative Propagation ◽  
Potassium Salt ◽  
Root Induction ◽  
First Flush ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Juglans Cinerea ◽  
Hardwood Species

Juglans cinerea L. (butternut) is a hardwood species valued for its wood and edible nuts. Information on the vegetative propagation of this species is currently unavailable. Our objective was to determine the conditions necessary for successful stem-cutting propagation of butternut. In 1999 and 2000, 10 trees (each year) were randomly selected from a 5- and 6-year-old butternut plantation located in Rosemount, Minn. Hardwood stem cuttings were collected in March, April, and May. Softwood cuttings were collected in June and July. K-IBA at 0, 29, or 62 mm in water and IBA at 0, 34, or 74 mm in 70% ethanol were tested for root induction on cuttings. The basal end of cuttings were dipped in a treatment solution for 10 to 15 seconds, potted in a peat: perlite mixture, and placed in a mist bed for 5 to 8 weeks. Rooted cuttings were gradually hardened off from the mist bed, allowed to initiate new growth, over-wintered in a controlled cold-storage environment, and then outplanted to the field. For hardwood cuttings, rooting was greatest for those taken in mid-May (branches flushed out), 22% with 62 mm K-IBA and 28% with 74 mm IBA. Softwood cuttings rooted best when taken in June (current season's first flush of new growth or softwood growth 40 cm or greater) and treated with 62 mm K-IBA (77%) or 74 mm IBA (88%). For 1999, 31 out of 51 rooted softwood cuttings (60.8%) survived overwintering in cold storage and acclimatization to the field. For 2000, 173 out of 186 rooted softwood cuttings (93%) survived overwintering and acclimatization to the field. Chemical names used: indole-3-butyric acid-potassium salt (K-IBA); indole-3-butyric acid (IBA).

scholarly journals Effect of Propagation Systems and Indole-3-Butyric Acid Potassium Salt (K-IBA) Concentrations on the Propagation of Peach Rootstocks by Stem Cuttings

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1151
Author(s):  
Ricardo A. Lesmes-Vesga ◽  
José X. Chaparro ◽  
Ali Sarkhosh ◽  
Mark A. Ritenour ◽  
Liliana M. Cano ◽  
...  
Keyword(s):  
Survival Rate ◽  
Root Growth ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Growth Parameters ◽  
Low Cost ◽  
Controlled Environment ◽  
Stem Cuttings ◽  
Use Of Resources ◽  
Peach Rootstocks

Traditionally, peach rootstocks are propagated by seeds due to their high availability, low cost, and easy storage and handling. However, stem cuttings allow the propagation of interspecific hybrids and keep the genetic uniformity of heterozygous genotypes. This study compared the effect of four different concentrations of K-IBA (indole-3-butyric acid potassium salt) on softwood cuttings of three peach backcrosses (peach × (peach × almond)) for rootstock propagation in two propagation systems: aeroponics and germination trays. The four concentrations of K-IBA applied were: 0.0% (w/v) as a control, 0.1% (w/v), 0.2% (w/v), and 0.4% (w/v). Data were collected on the survival rate (%), rooting rate (%), and root growth parameters. The relevance of auxin for peach cuttings rooting was evidenced. K-IBA at 0.2% showed the best rooting effect for peach softwood cuttings, evidenced by its high rooting rate and higher survival rate. K-IBA at 0.4% and 0.2% produced the highest number of adventitious roots. The highest root growth parameters were obtained in germination trays, confirming the suitability of this system for root growth. However, aeroponics was demonstrated to be as efficient as the traditional germination trays for the rooting of peach cuttings, allowing for a more controlled environment with a better use of resources.

scholarly journals CUTTING PROPAGATION WITH AUXIN APPLIED VIA THE ROOTING SUBSTRATE

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 884d-884
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
John M. Ruter
Keyword(s):  
Aqueous Solutions ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Organic Substrate ◽  
The Other ◽  
Species Number ◽  
Stem Cuttings ◽  
Rooted Cutting ◽  
Auxin Concentration ◽  
Euonymus Japonicus

Stem cuttings of golden euonymus (Euonymus japonicus `Aureo-marginatus'), shore juniper (Juniperus conferta `Blue Pacific'), white indian hawthorn (Rhaphiolepis indica `Alba'), and `Red Cascade' miniature rose (Rosa `Red Cascade') were successfully rooted in plugs of a stabilized organic substrate that had been soaked in aqueous solutions of the potassium salt of indole-3-butyric acid (K-IBA) at 0 to 75 mg·L–1 before inserting the cuttings. Cuttings were rooted under intermittent mist in polyethylene-covered greenhouses with rooting periods appropriate for each species. Rooting percentages showed some increase with increasing auxin concentration with juniper cuttings, but were similar among treatments for the other three species. Number of roots per rooted cutting increased with increasing auxin concentration with cuttings of juniper, Indian hawthorn, and rose, and was greatest using around 60 mg·L-1 K-IBA for cuttings of juniper and Indian hawthorn and 30 to 45 mg·L-1 K-IBA for cuttings of rose.

scholarly journals 101 Propagation of Juglans cinerea L. (Butternut)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 458F-459
Author(s):  
Paula M. Pijut ◽  
Melanie J. Barker
Keyword(s):  
High Pressure ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Root Induction ◽  
Rooted Cuttings ◽  
Canker Disease ◽  
Juglans Cinerea ◽  
Cutting Propagation ◽  
Bottom Heat ◽  
Collection Date

Butternut trees are becoming endangered as a result of butternut canker disease; thus, it is desirable to propagate disease-resistant trees for screening and provenance tests. The objective of this study was to determine the conditions necessary for successful cutting propagation. In 1998, 10 trees were selected from a 4-year-old butternut plantation located in Rosemount, Minn. Hardwood cuttings were collected 30 Mar., 21 Apr., and 6 May. The auxins, indole-3-butyric acid-potassium salt (KIBA) in water at 0, 29, or 74 mmol·L-1 were tested for root induction. The basal end of cuttings were dipped in treatment solutions for 10 to 15 s and placed in a 1 peat: 1 perlite mixture in Deepots™ (D40) in a mist bed. Mist was applied for 5 s every 15 min. Greenhouse conditions were: 12-h photoperiod provided by high-pressure sodium lamps (60 μmol·m-2·s-1), 22 °C, and bottom heat of 27 °C (heating pads). Softwood cuttings were collected 20 May, 18 June, 30 June, and 23 July. Rooting treatment solutions and greenhouse conditions were the same as for the hardwood cuttings, except no heating pads were used. Rooted cuttings were planted in Treepots™ (10 × 10 × 36 cm) and gradually hardened off from the mist bed. Hardwood cuttings from the first two collection dates did not initiate roots. Best rooting (12.5%) was achieved on hardwood cuttings collected 6 May using 29 mmol·L-1 KIBA. Softwood cuttings rooted to some degree at all concentrations of rooting solution and at every collection date. The greatest rooting (70%) was achieved using 74 mmol·L-1 IBA. In general, best rooting percentages were achieved with softwood cuttings collected 18 June and 23 July and treated with 62 mmol·L-1 KIBA or 74 mmol·L-1 IBA. Both rooted hardwood and softwood cuttings were successfully acclimatized from the mist bed and many have initiated new growth.

scholarly journals Vegetative Propagation of Peltophorum Petrocarpum (DC.) Backer ex K.Heyne a Multipurpose Tree

2014 ◽  
Vol 1 (2) ◽  
pp. 79-84
Author(s):  
Fadwa Mohammed Ahmed Ali ◽  
Yahia Hamid Ali Elbasheer
Keyword(s):  
Growth Performance ◽  
Biomass Production ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Large Scale ◽  
Tree Planting ◽  
Research Centre ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Mother Plants

This paper describes a successful protocol of vegetative propagation by using stem cuttings of mature Peltophorum petrocarpum plants. The experiment was conducted in the nursery of the Forestry Research Centre at Soba during November 2011and April 2012. The socioeconomic importance of P. petrocarpum comes from its multipurpose uses and there is a demand in production of its seed for tree planting and domestication activities. Hence there is a need for efficient and economically feasible propagation method of P. pterocarpum. Therefore the objective of study is to develop a vegetative propagation protocol by using rooted stem cuttings; and to determine the effects of the rooting hormone indole-3-butyric acid (IBA) on rooting potentiality of stem cuttings of mature mother plants. The result revealed a significant effect (p<0.001) of IBA concentrations on rooting percentage and growth performance of stem cuttings. The highest rooting percent was obtained with 200ppm IBA which was not significantly different from 100ppm, but significantly different from 50 and 0.0ppm. The effect of IBA concentrations on root lengths and of roots per cutting was significantly different from 0.0 and 50ppm treatments. Although 100ppm IBA gave the tallest root (4.6 cm) but it was not significantly different from 200ppm (4.3 cm). Survival of rooted cuttings after transplanting from the propagator was 100% and 97% with 100 and 200mg IBA respectively. Accordingly the conclusion reached is that rooted cuttings can be used successfully for obtaining health, uniform plantations of P. petrocarpum for large-scale, biomass production and tree planting programmes.   

scholarly journals Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality

2014 ◽  
Vol 42 (2) ◽  
pp. 478-481 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Giovanni IAPICHINO
Keyword(s):  
Acid Solution ◽  
Butyric Acid ◽  
Root Development ◽  
Root Length ◽  
Treatment Duration ◽  
Acid Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Root Quality

Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.

scholarly journals Effect of Bottom Heat, IBA Dipping Duration, and Wounding on Rooting of Stem Cuttings of Milicia excelsa

1997 ◽  
Vol 7 (1) ◽  
pp. 68-70 ◽  
Author(s):  
Yiqun Lin ◽  
Michael R. Wagner ◽  
Joseph R. Cobbinah
Keyword(s):  
Butyric Acid ◽  
Root Biomass ◽  
Dry Mass ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Bottom Heat ◽  
Milicia Excelsa

The effect of bottom heat, wounding, and duration of stem basal IBA dip on macropropagation of Milicia excelsa was investigated. Bottom heat enhanced root dry mass and accelerated root initiation. Percentage rooting and root dry mass were not affected by wounding and duration of stem basal treatment. However, wounding interacted with bottom heat to affect dry mass (P < 0.05). Root biomass was 60% higher from wounded cuttings than from nonwounded cuttings under the nonheated condition. Chemical names used: 1H-indole-3-butyric acid (IBA).

scholarly journals Propagation of Sweetgale, Rhodora, and Catberry by Stem Cuttings

2020 ◽  
Vol 30 (1) ◽  
pp. 38-46
Author(s):  
Bryan J. Peterson ◽  
Gregory J.R. Melcher ◽  
Ailish K. Scott ◽  
Rebecca A. Tkacs ◽  
Andrew J. Chase
Keyword(s):  
North America ◽  
Butyric Acid ◽  
Root Development ◽  
Vegetative Propagation ◽  
Potassium Salt ◽  
Eastern North America ◽  
Stem Cuttings ◽  
Razor Blade ◽  
Myrica Gale ◽  
Collection Date

Sweetgale (Myrica gale), rhodora (Rhododendron canadense), and catberry (Ilex mucronata) are shrubs of eastern North America that may have potential for broader use in horticultural landscapes. Because information on their vegetative propagation is scarce, we conducted experiments over 2 years to evaluate the effects of cutting collection date, wounding, substrate composition, and the concentration of applied potassium salt of indole-3-butyric acid (K-IBA) on rooting of each species. In 2015, we collected cuttings of each species on three dates to obtain both softwood and semihardwood cuttings. Cuttings were unwounded or wounded with a razor blade, and treated by dipping into water containing K-IBA at concentrations ranging from 0 to 15,000 mg·L−1, after which they were inserted into a substrate of 3:1 perlite:peat (by volume) and placed under intermittent mist. In 2016, semihardwood cuttings of each species were all wounded, treated with K-IBA from 0 to 15,000 mg·L−1, and inserted into substrates of 100%, 75%, or 50% perlite, with the remaining volume occupied by peat. In both years, the greatest percentage of sweetgale cuttings rooted when no K-IBA was applied. K-IBA application also reduced root ratings, root dry weights, and root lengths of sweetgale. For rhodora and catberry, maximal responses for all measures of rooting occurred when 5000 to 15,000 mg·L−1 K-IBA was applied. We recommend that growers use no exogenous auxin to propagate sweetgale, and 5000 to 10,000 mg·L−1 K-IBA to propagate rhodora and catberry. Cuttings of all three species can be collected from softwood or semihardwood shoots. Finally, sweetgale can be rooted in perlite alone, whereas rhodora and catberry required the addition of peatmoss for satisfactory root development.

Changes in growth, leaf abscission, and biomass associated with seasonal tropospheric ozone exposures of Populustremuloides clones and seedlings

1996 ◽  
Vol 26 (1) ◽  
pp. 23-37 ◽  
Author(s):  
D.F. Karnosky ◽  
Z.E. Gagnon ◽  
R.E. Dickson ◽  
M.D. Coleman ◽  
J.G. Isebrands ◽  
...  
Keyword(s):  
Tropospheric Ozone ◽  
Root Biomass ◽  
Biomass Accumulation ◽  
Wave Exposure ◽  
Leaf Abscission ◽  
Leaf Biomass ◽  
Diameter Growth ◽  
Rooted Cuttings ◽  
Upper Peninsula ◽  
Square Wave

The effects of single-season tropospheric ozone (O3) exposures on growth, leaf abscission, and biomass of trembling aspen (Populustremuloides Michx.) rooted cuttings and seedlings were studied. Plants were grown in the Upper Peninsula of Michigan in open-top chambers with O3 exposures that ranged from 7 to 92 ppm-h. Depending on the genotype, total seasonal O3 exposure in the range of 50–92 ppm-h had negative impacts on stem, retained leaf, and root biomass accumulation and on diameter growth. Leaf abscission generally increased with increasing O3 exposure and was the principal cause of the decrease in leaf biomass of the O3-treated plants. Considerable genetic variation in O3 responses occurred, as shown by differences in sensitivities among clones and among seedlings. However, the responses to O3 of rooted cuttings and seedlings were similar when seedling means were compared with clonal means for leaf abscission, diameter growth, retained leaf biomass, and root biomass. Comparison of a single square-wave treatment (52 ppm-h) with 70 and 92 ppm-h episodic exposures suggested that the plant response to the square-wave exposure was similar to the response to the highest episodic exposure even though the 92 ppm-h episodic exposure was almost twice the square-wave exposure. Our results are consistent with previous studies that show that P. tremuloides is highly responsive to O3 exposure and this response has a strong genetic component.

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