scholarly journals Effect of Propagation Systems and Indole-3-Butyric Acid Potassium Salt (K-IBA) Concentrations on the Propagation of Peach Rootstocks by Stem Cuttings

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1151
Author(s):  
Ricardo A. Lesmes-Vesga ◽  
José X. Chaparro ◽  
Ali Sarkhosh ◽  
Mark A. Ritenour ◽  
Liliana M. Cano ◽  
...  

Traditionally, peach rootstocks are propagated by seeds due to their high availability, low cost, and easy storage and handling. However, stem cuttings allow the propagation of interspecific hybrids and keep the genetic uniformity of heterozygous genotypes. This study compared the effect of four different concentrations of K-IBA (indole-3-butyric acid potassium salt) on softwood cuttings of three peach backcrosses (peach × (peach × almond)) for rootstock propagation in two propagation systems: aeroponics and germination trays. The four concentrations of K-IBA applied were: 0.0% (w/v) as a control, 0.1% (w/v), 0.2% (w/v), and 0.4% (w/v). Data were collected on the survival rate (%), rooting rate (%), and root growth parameters. The relevance of auxin for peach cuttings rooting was evidenced. K-IBA at 0.2% showed the best rooting effect for peach softwood cuttings, evidenced by its high rooting rate and higher survival rate. K-IBA at 0.4% and 0.2% produced the highest number of adventitious roots. The highest root growth parameters were obtained in germination trays, confirming the suitability of this system for root growth. However, aeroponics was demonstrated to be as efficient as the traditional germination trays for the rooting of peach cuttings, allowing for a more controlled environment with a better use of resources.

2018 ◽  
Vol 28 (3) ◽  
pp. 378-381
Author(s):  
Bryan J. Peterson ◽  
Stephanie E. Burnett ◽  
Olivia Sanchez

Although overhead mist revolutionized the propagation industry, it does suffer from potential drawbacks that include the application of large volumes of water, potentially unsanitary conditions, irregular misting coverage, and leaching of foliar nutrients. We explored the feasibility of submist as an alternative as it might avoid these problems by applying water exclusively from below the cutting, which is inserted basally into an enclosed rooting chamber. We propagated cuttings of korean lilac (Syringa pubescens ssp. patula) and inkberry (Ilex glabra) using both overhead mist and submist to compare effectiveness of the systems. Cuttings of korean lilac were wounded and dipped basally into 8000 mg·L−1 of the potassium salt of indole-3-butyric acid (K-IBA), and those in the overhead mist systems were inserted into coarse perlite. Cuttings of inkberry were wounded and treated with 5000 mg·L−1 K-IBA, and those in the overhead mist systems were inserted into 50:50 peat:perlite (by vol). Cuttings of korean lilac in the submist systems produced more than twice as many roots as cuttings in the overhead mist systems, with roots more than 2.6 times the length. Similarly, cuttings of inkberry in the submist systems produced more than three times the root counts and root lengths as cuttings in the overhead mist systems. For korean lilac, root dry weights averaged 58 mg for cuttings in the submist system, compared with only 18 mg among cuttings receiving overhead mist. Likewise, root dry weights averaged 70 and 7 mg for cuttings of inkberry propagated by submist and overhead mist, respectively. Rooted cuttings of korean lilac transplanted well into a soilless substrate, where they more than tripled their root biomass to 218 mg (vs. 59 mg for cuttings transplanted from overhead mist). We did not evaluate transplant performance of inkberry. Our results show that submist systems might merit consideration for the propagation of woody plants by leafy stem cuttings.


2019 ◽  
Vol 37 (2) ◽  
pp. 44-49
Author(s):  
Lois Braun ◽  
Donald Wyse

Abstract Hybrid hazelnuts are being developed as a new crop for the Upper Midwest for their ecological and economic value, but lack of economically viable propagation methods is a significant bottleneck to their wide scale adoption. In previous trials we found that hardwood stem cuttings could be propagated in low cost humidity tents constructed of molded plastic tubs covered with white 70% shade plastic. When the plastic was sealed tightly at the sides, these tubs maintained relative humidity near saturation, but also tended to overheat. This trial experimented with the use of ordinary household humidifiers as an alternative way of maintaining humidity while avoiding overheating. We found that it is not necessary to maintain RH near 100% as we had been doing when we kept the humidity tents tightly sealed. Stem survival and, as a consequence, rooting were improved in vented tents in which humidity was maintained with humidifiers, though these required much more management than the sealed tents. Index words: Propagation, Corylus americana (Walter), Corylus avellana (L.), rooting, indole-3-butyric acid Chemicals used in this study: indole-3-butyric acid (IBA) Species used in this study: hybrid hazelnuts [Corylus americana (Walter) x Corylus avellana (L.)]


2017 ◽  
Vol 14 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Mohammed Enamul Hoque

A field experiment was conducted at the central nursery of tea extension center of Bangladesh Tea Board in Bandarban during September to November, 2015 to determine the precise concentration of Indole Butyric Acid (IBA) on propagation through stem cuttings of tea. The experiment was laid out in a randomized block design with four treatments of IBA concentrations: 0 ppm (Control), 2000 ppm, 4000 ppm and 6000 ppm and each treatment was replicated four times. Basal part of tea (BT-2 variety) cuttings were dipped in different IBA concentrations for five seconds. Then these cuttings were dibbled into prepared soil mixture as rooting media. Cuttings were exposed to the rooting media for 90 days and then they all were removed carefully to assess the variation of establishment of cuttings. IBA treated cuttings showed better effect on the rooting rate, root length, root number, shoot length and survival rate compared to the control. It was noted that cuttings treated with 4000 ppm IBA produced the highest rooting rate 79.85%, the highest survival rate 57.60% and the highest root length of 4.85 cm followed by treatment 6000 ppm IBA. In case of all parameters, effects of the treatments 4000 ppm and 6000 ppm of IBA were statistically similar. Positive and strong correlation was observed both in rooting rate versus survival rate and root length versus shoot length in IBA treated cuttings. Thus, IBA at 4000 ppm concentration was recommended for establishment of stem cuttings of tea (BT-2 variety) in the nursery.The Agriculturists 2016; 14(2) 124-129


2019 ◽  
Vol 37 (1) ◽  
pp. 1-8
Author(s):  
Lois Braun ◽  
Donald Wyse

Abstract Germplasm improvement of woody crops through plant breeding depends on effective methods of vegetative propagation, both for evaluation of candidate germplasm and for dissemination of improved varieties. Hybrid hazelnuts [Corylus americana (Walter) x C. avellana (L.)] are being developed as a new crop for the Upper Midwest for their ecological and economic value, but lack of economically viable propagation methods is a significant bottleneck to their wide scale adoption. This paper describes two trials to optimize propagation from hardwood stem cuttings in low-cost humidity tents, using dormant crown suckers collected in the fall. We found that: 1) 2 g.L−1 (2,000 ppm) indole-3-butyric acid (IBA) in a 50% ethanol solution is the best concentration for rooting cuttings of a range of sizes and 2) rooting declines with increasing basal diameter of stems, but increases with increasing length of stem. The thicker cortex of large diameter stems may present a physical barrier to emergence of adventitious roots. Conversely, longer stems have larger stores of carbohydrates, which are essential both for keeping the stems alive until roots can be developed, and for providing the building blocks for growing those roots. Index words: Propagation, Corylus americana (Walter), Corylus avellana (L.), rooting, indole-3-butyric acid. Chemicals used in this study: indole-3-butyric acid (IBA). Species used in this study: hybrid hazelnuts [Corylus americana (Walter) x Corylus avellana (L.)].


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 884d-884
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
John M. Ruter

Stem cuttings of golden euonymus (Euonymus japonicus `Aureo-marginatus'), shore juniper (Juniperus conferta `Blue Pacific'), white indian hawthorn (Rhaphiolepis indica `Alba'), and `Red Cascade' miniature rose (Rosa `Red Cascade') were successfully rooted in plugs of a stabilized organic substrate that had been soaked in aqueous solutions of the potassium salt of indole-3-butyric acid (K-IBA) at 0 to 75 mg·L–1 before inserting the cuttings. Cuttings were rooted under intermittent mist in polyethylene-covered greenhouses with rooting periods appropriate for each species. Rooting percentages showed some increase with increasing auxin concentration with juniper cuttings, but were similar among treatments for the other three species. Number of roots per rooted cutting increased with increasing auxin concentration with cuttings of juniper, Indian hawthorn, and rose, and was greatest using around 60 mg·L-1 K-IBA for cuttings of juniper and Indian hawthorn and 30 to 45 mg·L-1 K-IBA for cuttings of rose.


2020 ◽  
Vol 38 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Ping-Cheng Hou ◽  
Kuan-Hung Lin ◽  
Yen-Jung Huang ◽  
Chun-Wei Wu ◽  
Yu-Sen Chang

ABSTRACT The objective of our study was to develop a protocol enabling the use of vegetation indices to evaluate the rooting of Azalea (Rhododendron pulchrum cv. Sweet) cuttings. Six root growth parameters were recorded after exposing those cuttings to rooting media for 47 days. Among plants with different soil-plant analysis development (SPAD) and normalized difference vegetation index (NDVI) values, those with higher values exhibited significantly higher number of roots, root length, and root dry weight, suggesting that reflectance indices were useful in measuring the root growth parameters of the cuttings. Another aim of this work was to study the effects of plant growth regulators (PGRs) on rooting for cutting propagation. Azalea cuttings were soaked in the treatments with indole-3-butyric acid (IBA) at 2,000 mg L-1 or combined with 1-naphthaleneacetic acid (NAA; 2,000 mg L-1), salicylic acid (SA; 10-4 M), and thiamine (TA; 800 mg L-1). The same observations as SPAD and NDVI on six different rooting parameters were recorded and analyzed after cutting’s exposure to rooting media for 99 days for the auxin test, and 62 days for the SA and TA tests. Compared to NAA alone, IBA enhanced root growth and development as determined by increases in all parameters, and therefore it was used thereafter. Successful results for the number of roots and root dry weight were achieved using azalea cuttings with a combination of IBA and SA. In addition, the mix of IBA and TA resulted in higher number of roots and length of root. These combined treatments are recommended for establishing stem cuttings to produce nursery plants of azalea.


HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 697-700 ◽  
Author(s):  
Paula M. Pijut ◽  
Melanie J. Moore

Juglans cinerea L. (butternut) is a hardwood species valued for its wood and edible nuts. Information on the vegetative propagation of this species is currently unavailable. Our objective was to determine the conditions necessary for successful stem-cutting propagation of butternut. In 1999 and 2000, 10 trees (each year) were randomly selected from a 5- and 6-year-old butternut plantation located in Rosemount, Minn. Hardwood stem cuttings were collected in March, April, and May. Softwood cuttings were collected in June and July. K-IBA at 0, 29, or 62 mm in water and IBA at 0, 34, or 74 mm in 70% ethanol were tested for root induction on cuttings. The basal end of cuttings were dipped in a treatment solution for 10 to 15 seconds, potted in a peat: perlite mixture, and placed in a mist bed for 5 to 8 weeks. Rooted cuttings were gradually hardened off from the mist bed, allowed to initiate new growth, over-wintered in a controlled cold-storage environment, and then outplanted to the field. For hardwood cuttings, rooting was greatest for those taken in mid-May (branches flushed out), 22% with 62 mm K-IBA and 28% with 74 mm IBA. Softwood cuttings rooted best when taken in June (current season's first flush of new growth or softwood growth 40 cm or greater) and treated with 62 mm K-IBA (77%) or 74 mm IBA (88%). For 1999, 31 out of 51 rooted softwood cuttings (60.8%) survived overwintering in cold storage and acclimatization to the field. For 2000, 173 out of 186 rooted softwood cuttings (93%) survived overwintering and acclimatization to the field. Chemical names used: indole-3-butyric acid-potassium salt (K-IBA); indole-3-butyric acid (IBA).


2020 ◽  
Vol 30 (1) ◽  
pp. 38-46
Author(s):  
Bryan J. Peterson ◽  
Gregory J.R. Melcher ◽  
Ailish K. Scott ◽  
Rebecca A. Tkacs ◽  
Andrew J. Chase

Sweetgale (Myrica gale), rhodora (Rhododendron canadense), and catberry (Ilex mucronata) are shrubs of eastern North America that may have potential for broader use in horticultural landscapes. Because information on their vegetative propagation is scarce, we conducted experiments over 2 years to evaluate the effects of cutting collection date, wounding, substrate composition, and the concentration of applied potassium salt of indole-3-butyric acid (K-IBA) on rooting of each species. In 2015, we collected cuttings of each species on three dates to obtain both softwood and semihardwood cuttings. Cuttings were unwounded or wounded with a razor blade, and treated by dipping into water containing K-IBA at concentrations ranging from 0 to 15,000 mg·L−1, after which they were inserted into a substrate of 3:1 perlite:peat (by volume) and placed under intermittent mist. In 2016, semihardwood cuttings of each species were all wounded, treated with K-IBA from 0 to 15,000 mg·L−1, and inserted into substrates of 100%, 75%, or 50% perlite, with the remaining volume occupied by peat. In both years, the greatest percentage of sweetgale cuttings rooted when no K-IBA was applied. K-IBA application also reduced root ratings, root dry weights, and root lengths of sweetgale. For rhodora and catberry, maximal responses for all measures of rooting occurred when 5000 to 15,000 mg·L−1 K-IBA was applied. We recommend that growers use no exogenous auxin to propagate sweetgale, and 5000 to 10,000 mg·L−1 K-IBA to propagate rhodora and catberry. Cuttings of all three species can be collected from softwood or semihardwood shoots. Finally, sweetgale can be rooted in perlite alone, whereas rhodora and catberry required the addition of peatmoss for satisfactory root development.


2000 ◽  
Vol 18 (4) ◽  
pp. 185-187
Author(s):  
Jeffrey G. Norcini ◽  
James H. Aldrich

Abstract Helianthus debilis Nutt. ssp. debilis ‘Flora Sun’, ‘Flora Sun’ beach sunflower, is a native, herbaceous perennial that should be propagated by cuttings to maintain its genetic and phenotypic fidelity. Stem cuttings were treated with a quick dip of a potassium salt of 1H-indole-3-butyric acid (K-IBA) solution at 0, 250, 500, 1000, 1500, 2000, 2500, or 3000 mg/liter (ppm) and rooted under intermittent mist. The root systems of six of twelve cuttings were harvested 17 and 21 days after treatment. The remaining six cuttings were transplanted in a soilless substrate to determine the effects of K-IBA on establishment in containers. Treatment of stem cuttings with K-IBA increased the number of roots and root fresh weight when cuttings were rooted under summer greenhouse conditions, but K-IBA did not provide any substantial short-term benefits in survival or growth of rooted cuttings after they were transplanted into plastic containers.


2021 ◽  
pp. 096100062110165
Author(s):  
Mohammadhiwa Abdekhoda ◽  
Fatemeh Ranjbaran ◽  
Asghar Sattari

This study was conducted with the aim of evaluating the role of information and information resources in the awareness, control, and prevention of COVID-19. This study was a descriptive-analytical survey in which 450 participants were selected for the study. The data collection instrument was a researcher-made questionnaire. Descriptive and inferential statistics were used to analyze the data through SPSS. The findings show that a wide range of mass media has become well known as information resources for COVID-19. Other findings indicate a significant statistical difference in the rate of using information resources during COVID-19 based on age and gender; however, this difference is not significant regarding the reliability of information resources with regard to age and gender. Health information has an undisputable role in the prevention and control of pandemic diseases such as COVID-19. Providing accurate, reliable, and evidence-based information in a timely manner for the use of resources and information channels related to COVID-19 can be a fast and low-cost strategic approach in confronting this disease.


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