scholarly journals Vegetative Growth of Three Southern Highbush Blueberry Cultivars Obtained from Micropropagation and Softwood Cuttings in Two Florida Locations

HortScience ◽  
2014 ◽  
Vol 49 (5) ◽  
pp. 556-561 ◽  
Author(s):  
Silvia R. Marino ◽  
Jeffrey G. Williamson ◽  
James W. Olmstead ◽  
Philip F. Harmon

Field performance of southern highbush blueberry (Vaccinium corymbosum L. interspecific hybrids) cultivars Emerald, Jewel, and Primadonna derived from softwood cuttings (SW) and tissue culture (TC) was evaluated in Citra and Haines City, FL, in 2010–12. Both fields were planted in Apr. 2010 on sandy soil amended with pine bark. Plant height and width were recorded at both locations, from which plant canopy volume was calculated. Additionally, whole plants were harvested at planting and after the first growing season, after the first fruit harvest, and after the second growing season. Average plant height and width, number of major canes, and total shoot number were determined at each sampling date. Dry weights for roots, crowns, canes, shoots, and leaves were obtained. Although propagation method affected plant canopy volume during the first season, no effects were observed by the end of the second growing season. At planting and after the first and second growing seasons, TC plants of the three cultivars had more major canes. Total shoot number per plant was greater for TC ‘Jewel’ at all dates but ‘Emerald’ TC plants had more shoots only at planting and after the first growing and harvest seasons. Tissue culture resulted in increased plant dry weights of ‘Jewel’ and ‘Emerald’ after the first and second growing seasons. There were no significant differences in total number of shoots or plant dry weight between TC and SW-derived ‘Primadonna’ plants at any point during the study.

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1033B-1033
Author(s):  
Nicole E. Burkhard ◽  
Derek H. Lynch ◽  
David C. Percival

Within-row weed management of highbush blueberry (Vaccinium corymbosum L.) is reliant upon herbicide applications. However, in organic production, herbicides are typically not permitted and alternative methods must be used. The impact of thick (25-cm) mulch applications on weed pressure in an organic production system was initiated at a commercial operation in Nova Scotia, Canada, during 2005. A split-plot experimental design was used with five blocks (replications), six treatments, and five plants per split plot (cv. Duke). The whole-plot factor consisted of mulch/fertility treatments and included: i) control (no amendment); ii) ammonium sulphate fertilizer (30 kg·ha-1 N); iii) pelletized poultry manure (60 kg·ha-1 N); iv) pine needles (80 t·ha-1); v) horse manure and sawdust compost (550 t·ha-1); and vi) seafood waste compost (360 t·ha-1). The split-plot factor consisted of level of hand weeding (–/+). Weed control was assessed by sampling percent ground cover and weed shoot biomass in three 0.25-m2 quadrats in nonweeded subplots. Blueberry leaf N content, plant canopy volume, and berry yield (fresh weight and number) were recorded. The manure/sawdust compost and pine needle treatments had the lowest weed biomass and percent ground cover values, thereby providing the best weed control. Weed shoot biomass, blueberry leaf N, plant canopy volume, and berry yield were greatest in the seafood waste compost treatment. Results from this preliminary study indicate the potential of using these groundcover treatments to improve organic cultural management practices.


HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 880-886 ◽  
Author(s):  
Bruno Casamali ◽  
Rebecca L. Darnell ◽  
Alisson P. Kovaleski ◽  
James W. Olmstead ◽  
Jeffrey G. Williamson

Vaccinium arboreum Marsh is a wild species adapted to high pH (above 6.0) and low organic matter soils (below 2.0%). The use of V. arboreum rootstocks may be a viable option to increase soil adaptation of southern highbush blueberry (SHB) (Vaccinium corymbosum interspecific hybrid) under marginal soil conditions. The objective of this research was to evaluate the vegetative and reproductive traits of ‘Farthing’ and ‘Meadowlark’ SHB own-rooted or grafted onto V. arboreum and grown in pine bark–amended or nonamended soil. The study was conducted from 2012 through 2014 at a research center in Citra, FL, and a grower’s farm in Archer, FL. Vaccinium arboreum rootstock generally induced the same effects in both cultivars. Grafted plants in both soil treatments had reduced canopy growth in the first year after field planting compared with own-rooted plants in amended soil. However, canopy volume of grafted plants was greater than own-rooted plants in nonamended soil and similar to own-rooted plants in amended soil 2 years after field planting for ‘Meadowlark’ and 3 years after planting for ‘Farthing’. Fruit yield was lower in grafted plants compared with own-rooted plants in the first fruiting year (2 years after field planting). By the second fruiting year, yields of grafted plants were similar to or greater than yields of own-rooted plants when grown in nonamended soil, whereas in amended soil, yields of grafted plants were similar to yields of own-rooted plants. Grafted plants had greater mean berry weight, but lower berry firmness; however, the firmness values were still considered acceptable (greater than 160 g⋅mm−1). Internal fruit quality [total soluble solids (TSS) and total titratable acidity (TTA)] was not consistently affected by the rootstock or soil treatments. These results suggest that grafting SHB onto V. arboreum does not increase yield in the establishment years compared with own-rooted SHB when grown in amended soils, but may have the ability to increase yield with no negative effects on fruit quality when grown in nonamended soils.


HortScience ◽  
2015 ◽  
Vol 50 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Alisson P. Kovaleski ◽  
Jeffrey G. Williamson ◽  
Bruno Casamali ◽  
Rebecca L. Darnell

Pruning is a recommended practice for blueberry (Vaccinium spp.) production and is usually done in the summer in warm subtropical climates with long growing seasons. Summer pruning promotes healthy vegetative growth during the remainder of the growing season; however, research-based recommendations for summer pruning strategies are lacking. The objective of this study was to determine effects of summer pruning timing and intensity on vegetative growth in ‘Jewel’ and ‘Emerald’ southern highbush blueberry (V. corymbosum-interspecific hybrid), two cultivars of the primary species grown in subtropical areas. To determine effects of pruning time, 30% of the canopy was removed in June or July. To determine pruning intensity effects, either 30% or 60% of the canopy was removed in June, both followed by shoot tipping in July. Both timing and intensity treatments were compared with a non-pruned control. Lack of pruning in the first year had no negative effects on growth; however, lack of pruning for two or more seasons decreased regrowth volume and shoot length of both cultivars. By the third season, canopy regrowth volume in both cultivars decreased in the non-pruned control compared with the 30% and 60% pruning treatments and compared with the June pruning treatment. Disease infection in ‘Jewel’ was also increased in the non-pruned control compared with these pruning treatments. Summer pruning, regardless of timing or intensity, generally increased vigor of vegetative growth for both cultivars and decreased incidence of leaf disease in ‘Jewel’.


2015 ◽  
Vol 140 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Alisson P. Kovaleski ◽  
Jeffrey G. Williamson ◽  
James W. Olmstead ◽  
Rebecca L. Darnell

Blueberry (Vaccinium spp.) production is increasing worldwide, particularly in subtropical growing regions, but information on timing and extent of inflorescence bud development during summer and fall and effects on bloom the next season are limited. The objectives of this study were to determine time of inflorescence bud initiation, describe internal inflorescence bud development, and determine the relationship between internal inflorescence bud development and bloom period the next spring in two southern highbush blueberry [SHB (Vaccinium corymbosum interspecific hybrids)] cultivars. ‘Emerald’ and ‘Jewel’ SHB buds were collected beginning in late summer until shoot growth cessation in late fall for dissection and identification of organ development. Inflorescence bud frequency and number, vegetative and inflorescence bud length and width throughout development, and bloom were also assessed. Inflorescence bud initiation occurred earlier in ‘Emerald’ compared with ‘Jewel’. Five stages of internal inflorescence bud development were defined throughout fall in both cultivars, ranging from a vegetative meristem to early expansion of the inflorescence bud in late fall. ‘Emerald’ inflorescence buds were larger and bloomed earlier, reflecting the earlier inflorescence bud initiation and development. Although inflorescence bud initiation occurred earlier in ‘Emerald’ compared with ‘Jewel’, the pattern of development was not different. Timing of inflorescence bud initiation influenced timing of bloom with earlier initiation resulting in earlier bloom.


HortScience ◽  
2012 ◽  
Vol 47 (12) ◽  
pp. 1745-1749 ◽  
Author(s):  
Anish Malladi ◽  
Tripti Vashisth ◽  
Lisa Klima Johnson

Two abscission agents, ethephon and methyl jasmonate, were investigated in five studies to determine their potential for increasing fruit detachment during harvest in rabbiteye (Vaccinium ashei Reade) and southern highbush (hybrids based largely on Vaccinium corymbosum L. and Vaccinium darrowi Camp.) blueberry. In the first study with a rabbiteye blueberry genotype, T-451, ethephon applications up to 1000 mg·L−1 did not affect fruit drop but reduced fruit detachment force (FDF) by up to 21%. In the second study with two southern highbush blueberry genotypes, ethephon (up to 1500 mg·L−1) and methyl jasmonate (MeJa; up to 10 mm) applications resulted in significant fruit drop in ‘Star’ but neither of the growth regulators affected the fruit detachment characteristics of ‘Farthing’. In a third study with rabbiteye blueberry genotypes, MeJa applications of 10, 20, and 30 mm displayed an increasing linear trend in fruit drop in ‘Climax’ and linear and quadratic trends in fruit drop in ‘Powderblue’. In a fourth study with ‘Powderblue’, MeJa (20 mm) and ethephon (1000 mg·L−1) applications resulted in rapid and significant fruit drop. The fruit drop induced by MeJa in this study was attenuated by the coapplication of aminoethoxyvinylglycine (AVG), an ethylene biosynthesis inhibitor, suggesting that MeJa induced fruit detachment partly through its effects on ethylene biosynthesis. In another study with the southern highbush blueberry genotype, O’Neal, MeJa applications (20 mm) induced significant fruit drop but ethephon (1000 mg·L−1) applications did not affect fruit detachment. Overall, MeJa applications (20 mm or greater) generally induced rapid and extensive fruit abscission, often within 1 day after treatment. Applications of MeJa resulted in leaf yellowing and necrosis of leaf tips and margins, especially at high rates of application (20 mm or greater). Ethephon applications resulted in the abscission of mature and immature berries. Both ethephon and MeJa applications resulted in the detachment of the pedicel along with the fruit. Together, these data suggest that although ethephon and MeJa have the potential to be used as harvest aids in blueberry, the rates of application require further optimization to minimize potential phytotoxicity. Additionally, effective de-stemming of the berries may be essential if these compounds are to be used as harvest aids.


HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1127-1129 ◽  
Author(s):  
Lisa J. Rowland ◽  
Elizabeth L. Ogden

Conditions for improving the efficiency of shoot regeneration from leaf sections of highbush blueberry (Vaccinium corymbosum L.) were investigated. Effectiveness of tissue culture medium supplemented with the cytokinin conjugate zeatin riboside or the cytokinin zeatin at 10, 20, or 30 μm was compared with medium supplemented with the optimum 2iP concentration of 15 μm. Use of 20 μm zeatin riboside resulted in the most shoots per leaf section, » 6-fold higher than the number of shoots produced on 2iP medium. The number of shoots produced on medium supplemented with zeatin was not significantly higher than the number of shoots produced on 2iP medium. Consequently, we concluded that the cytokinin conjugate zeatin riboside was more effective than either of the free cytokinins, 2iP or zeatin, in promoting shoot regeneration from leaf sections of highbush blueberry. Chemical names used: 6-(y,y-dimethylallylamino)-purine (2iP); 6-(4-hydroxy-3-methyl-but-2-enylamino)purine (zeatin).


HortScience ◽  
2013 ◽  
Vol 48 (7) ◽  
pp. 835-843 ◽  
Author(s):  
Jessica L. Gilbert ◽  
Michael L. Schwieterman ◽  
Thomas A. Colquhoun ◽  
David G. Clark ◽  
James W. Olmstead

Previously, when selecting for flavor in the University of Florida southern highbush blueberry (SHB, Vaccinium corymbosum L. hybrids) breeding program, sugar/acid ratios and breeder preference were the only factors considered. A more precise method of evaluating flavor would include volatile compounds that may also contribute to the flavor experience. Therefore, volatile profiles of five SHB cultivars (Farthing, FL01-173, Scintilla, Star, and Sweetcrisp) were compared using gas chromatography–mass spectrometry. All cultivars were harvested on four separate dates within the harvest season, and fruit from each cultivar were also harvested at four developmental stages on the first harvest date. Among the cultivars, soluble solids content and volatile production tended to increase with fruit maturity, whereas titratable acidity decreased. All volatile components were more variable than measures of sugars and acids during the harvest season. Many of the volatiles present varied significantly between harvest dates, resulting in significant genotype × environment interactions during the harvest season. A closer examination of linalool, trans-2-hexenol, trans-2-hexenal, hexanal, and 1-penten-3-ol, five volatile compounds commonly associated with blueberry flavor, showed cultivar, developmental stage, and harvest date differences for each volatile. ‘Star’ experienced the least variation through the harvest period.


2015 ◽  
Vol 25 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Jeffrey G. Williamson ◽  
Luis Mejia ◽  
Bradley Ferguson ◽  
Paul Miller ◽  
Dorota Z. Haman

Nonweighing drainage lysimeters were used to measure seasonal water use of mature ‘Emerald’ southern highbush blueberry (SHB; Vaccinium corymbosum interspecific hybrid) plants grown in pine bark beds and in pine bark amended soil in north central Florida. In the absence of rain, irrigation was applied daily with microsprinklers at ≈120% to 175% of reference evapotranspiration as either single or split applications. Leachate was collected and its volume determined from each lysimeter at 6- to 10-day intervals throughout the study. Water use, expressed as L/plant, was calculated as the difference between the amount of irrigation/rain added to lysimeters and the amount of leachate collected from lysimeters during each measurement period. Average daily water use was calculated for monthly intervals beginning in Apr. 2010 and ending in Sept. 2012. Water use increased rapidly during spring through the final stages of fruit ripening and harvest (May) with peak water use occurring during mid to late summer (July, August, and September). Plants grown in pine bark beds used more water than plants in pine bark amended soil during Apr., May, and Dec. 2010, Feb. 2011, and Mar. 2012, but there were no differences during the periods of highest water use. No differences in water use were observed between single or split-application irrigation treatments. Monthly averages for daily water use during the 30-month period ranged from ≈1.75 L/plant in January to ≈8.0 L/plant in mid to late summer. Mean monthly crop coefficient values during the 30-month period ranged from 0.44 in February to 0.86 in September. Canopy volume, yield, and mean berry weight were unaffected by soil or irrigation treatments.


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