scholarly journals Regeneration of Babaco (Carica pentagona) from Ovular Callus

1991 ◽  
Vol 116 (4) ◽  
pp. 747-752 ◽  
Author(s):  
R. Vega de Rojas ◽  
S.L. Kitto
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Embryo Conversion ◽  
Fluorescent Lamps ◽  
Green Areas ◽  
Half Strength ◽  
Indole 3 Acetic Acid

Ovules of babaco [Carica pentagona (Heilborn) Badillo], 23 to 140 days old, were cultured to initiate regenerative callus. Callus developed from the integuments and possibly from the nucellus. Ovules of greater length and age produced more calli on White's medium or medium with half-strength MS salts than on full-strength MS. Ovules >60 days old that were chilled for 24 hours produced significantly more callus than fresh ovules <60 days old. Ovular calli of summer and fall fruits (73 to 90 days old) grown at 23 ± 2C under cool-white fluorescent lamps (16- or 18-hour photoperiod, 12 or 16 μmol·s-1·m-2) developed green areas that subsequently produced nodular structures. Nodular structures produced proembryonal structures that developed into mature somatic embryos when transferred to media containing either GA3 (0.1 mg·liter-1) plus activated charcoal (2.0 g·liter-1) or casein hydrolysate (200 mg·liter-1) plus IAA (0.5 mg·liter-1). Somatic embryos converted into plantlets when transferred to embryo conversion medium. Chemical names used: 1-H -indole-3-acetic acid (IAA); gibberellic acid (GA3).

Dendrobium Hybrid Flower Number and Pedicel Curvature is Influenced by the Application of Gibberellic Acid and Indole-3-acetic Acid

2021 ◽  
Vol 48 (6) ◽  
pp. 740-745
Author(s):  
Muhammad-Asyraf Khairul-Anuar ◽  
Purabi Mazumdar ◽  
Sam Lum ◽  
Jennifer Ann Harikrishna
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Flower Number ◽  
Indole 3 Acetic Acid

scholarly journals Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 374 ◽  
Author(s):  
Chang Park ◽  
Hyeon Yeo ◽  
Yun Park ◽  
Abubaker Morgan ◽  
Mariadhas Valan Arasu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Fagopyrum Esculentum Moench ◽  
Indole 3 Acetic Acid

scholarly journals Micropropagation of Butea buteiformis (Voigt) Grierson and Long

2007 ◽  
Vol 7 ◽  
pp. 119
Author(s):  
Belai Meeta Singh ◽  
Cristoph Wawrosch ◽  
Sanu Devi Joshi ◽  
Brigitte Kopp
Keyword(s):  
Acetic Acid ◽  
Analytical Procedure ◽  
Science And Technology ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Mature Seeds ◽  
Indole 3 Acetic Acid

Mature seeds of Butea buteiformis were cultured on half strength Murashige and Skoog (1962) medium. Nodal cuttings were used as explants from in vitro growing plants for experimentation. Plants were well grown on the medium supplemented with benzylaminopurine (BAP) 0.5 µM / l and indole-3-acetic acid (IAA) 1.0 µM/ l. Such propagated plants were acclimatized very well and transferred to the field. All the collected data were worked out statistically with SPSS, a system of analytical procedure. <i>Nepal Journal of Science and Technology</i> Vol. 7, 2006

scholarly journals Pomegranate Micropropagation, Callogenesis and Genetic Integrity Assessment Using Simple Sequence Repeat Markers

2018 ◽  
Vol 11 (1) ◽  
pp. 237
Author(s):  
Tebogo Stimela ◽  
Remmy W. Kasili ◽  
Edward G. Mamati
Keyword(s):  
Acetic Acid ◽  
Simple Sequence Repeat ◽  
Naphthaleneacetic Acid ◽  
Genetic Integrity ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Simple Sequence

In recent years, the awareness of pomegranate health benefits has grown exponentially; nonetheless the existing propagation methods remain a challenge to supply adequate suitable planting materials needed for commercial production. Micropropagation can lead to mass production of plantlets and callus-mediated in vitro regeneration can open avenues for the use of genetic engineering to improve this crop. The aim of this study was to evaluate appropriate conditions for pomegranate micropropagation, callogenesis and use Simple Sequence Repeat markers to screen for somaclonal variation. Cytokinins (Benzylaminopurine, Kinetin and Thiadiazol-5ylurea) were tested for shoot induction from nodal explants while auxins (1-Naphthaleneacetic acid, Indole-3-butyric acid and Indole-3-acetic acid) were tested for root induction of in vitro regenerated shoots. 1-Naphthaleneacetic acid combined with Benzylaminopurine was assessed for their ability to induce callus from cotyledon and leaf explants. Genetic integrity between mother plant, callus and in vitro regenerated shoots were assessed using eight Simple Sequence Repeat markers. Maximum number of shoots and leaves were obtained on full strength Murashige and Skoog media with 6.9 &micro;M kinetin. The highest number of roots was achieved on half strength Murashige and Skoog media with 4.9 &micro;M Indole-3-butyric acid and the longest root was got on half strength Murashige and Skoog media with 5.3 &micro;M Indole-3-acetic acid. Leaves and cotyledons demonstrated to be potential explants for callus formation at all hormonal combination levels tested. Eight out of 13 amplified alleles were polymorphic. A wider genetic variation was found with similarity coefficient range of 0.46-0.92. More somaclonal variation was in regenerated shoots compared to callus.

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