scholarly journals Isolation and Functional Analysis of Flowering Locus T in Tree Peonies (PsFT)

2015 ◽  
Vol 140 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Hua Zhou ◽  
Fang-Yun Cheng ◽  
Jing Wu ◽  
Chaoying He
Keyword(s):  
Flowering Time ◽  
Woody Plants ◽  
Potential Role ◽  
Expression Patterns ◽  
Wild Type ◽  
Coding Region ◽  
Flowering Locus T ◽  
Wide Range ◽  
Flowering Locus ◽  
Ornamental Shrub

Arabidopsis thaliana Flowering locus T (FT) homologs have been shown to be sufficient to trigger flowering and to regulate flowering time in a wide range of plants. However, such a homologue for the perennial ornamental shrub tree peony has not yet been characterized. In this study, we isolated PsFT, which is a closely related FT homolog from reblooming [Paeonia ×lemoinei ‘High Noon’ (HN)] and nonreblooming [P. ×suffruticosa ‘Luo Yang Hong’ (LYH)] cultivars of tree peonies, and identified its potential role in the regulation of flowering time. The PsFT alleles from the two cultivars encode the same protein, which indicates that the polymorphisms observed in the coding region do not contribute to the distinct flowering phenotypes of HN and LYH. Comparative analyses of the PsFT expression patterns in HN and LYH indicated that PsFT might be associated with reblooming. Transgenic A. thaliana plants ectopically expressing PsFT exhibited a phenotype that included significantly early flowering compared with the wild-type (WT) plants. Taken together, our data provide valuable clues for shortening the juvenile periods and extending the flowering periods of perennial woody plants, such as tree peonies.

scholarly journals A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape

2021 ◽  
Author(s):  
Paul Vollrath ◽  
Harmeet S. Chawla ◽  
Sarah V. Schiessl ◽  
Iulian Gabur ◽  
HueyTyng Lee ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Oilseed Rape ◽  
Major Effect ◽  
Structural Variants ◽  
Winter Oilseed Rape ◽  
Flowering Locus T ◽  
Winter Type ◽  
Long Read ◽  
Flowering Locus

Abstract Key message A novel structural variant was discovered in the FLOWERING LOCUS T orthologue BnaFT.A02 by long-read sequencing. Nested association mapping in an elite winter oilseed rape population revealed that this 288 bp deletion associates with early flowering, putatively by modification of binding-sites for important flowering regulation genes. Abstract Perfect timing of flowering is crucial for optimal pollination and high seed yield. Extensive previous studies of flowering behavior in Brassica napus (canola, rapeseed) identified mutations in key flowering regulators which differentiate winter, semi-winter and spring ecotypes. However, because these are generally fixed in locally adapted genotypes, they have only limited relevance for fine adjustment of flowering time in elite cultivar gene pools. In crosses between ecotypes, the ecotype-specific major-effect mutations mask minor-effect loci of interest for breeding. Here, we investigated flowering time in a multiparental mapping population derived from seven elite winter oilseed rape cultivars which are fixed for major-effect mutations separating winter-type rapeseed from other ecotypes. Association mapping revealed eight genomic regions on chromosomes A02, C02 and C03 associating with fine modulation of flowering time. Long-read genomic resequencing of the seven parental lines identified seven structural variants coinciding with candidate genes for flowering time within chromosome regions associated with flowering time. Segregation patterns for these variants in the elite multiparental population and a diversity set of winter types using locus-specific assays revealed significant associations with flowering time for three deletions on chromosome A02. One of these was a previously undescribed 288 bp deletion within the second intron of FLOWERING LOCUS T on chromosome A02, emphasizing the advantage of long-read sequencing for detection of structural variants in this size range. Detailed analysis revealed the impact of this specific deletion on flowering-time modulation under extreme environments and varying day lengths in elite, winter-type oilseed rape.

scholarly journals Isolation and Functional Characterization of MsFTa, a FLOWERING LOCUS T Homolog from Alfalfa (Medicago sativa)

2019 ◽  
Vol 20 (8) ◽  
pp. 1968 ◽  
Author(s):  
Junmei Kang ◽  
Tiejun Zhang ◽  
Tao Guo ◽  
Wang Ding ◽  
Ruicai Long ◽  
...  
Keyword(s):  
Flowering Time ◽  
Functional Characterization ◽  
Flowering Locus T ◽  
Over Expression ◽  
Long Day ◽  
Gfp Fusion Protein ◽  
Intron Structure ◽  
Flowering Locus ◽  
Flowering Regulation

The production of hay and seeds of alfalfa, an important legume forage for the diary industry worldwide, is highly related to flowering time, which has been widely reported to be integrated by FLOWERING LOCUS T (FT). However, the function of FT(s) in alfalfa is largely unknown. Here, we identified MsFTa, an FT ortholog in alfalfa, and characterized its role in flowering regulation. MsFTa shares the conserved exon/intron structure of FTs, and MsFTa is 98% identical to MtFTa1 in Medicago trucatula. MsFTa was diurnally regulated with a peak before the dark period, and was preferentially expressed in leaves and floral buds. Transient expression of MsFTa-GFP fusion protein demonstrated its localization in the nucleus and cytoplasm. When ectopically expressed, MsFTa rescued the late-flowering phenotype of ft mutants from Arabidopsis and M. trucatula. MsFTa over-expression plants of both Arabidopsis and M. truncatula flowered significantly earlier than the non-transgenic controls under long day conditions, indicating that exogenous MsFTa strongly accelerated flowering. Hence, MsFTa functions positively in flowering promotion, suggesting that MsFTa may encode a florigen that acts as a key regulator in the flowering pathway. This study provides an effective candidate gene for optimizing alfalfa flowering time by genetically manipulating the expression of MsFTa.

scholarly journals Dehydroabietinal promotes flowering time and plant defense in Arabidopsis via the autonomous pathway genes FLOWERING LOCUS D, FVE, and RELATIVE OF EARLY FLOWERING 6

2020 ◽  
Vol 71 (16) ◽  
pp. 4903-4913 ◽  
Author(s):  
Zulkarnain Chowdhury ◽  
Devasantosh Mohanty ◽  
Mrunmay K Giri ◽  
Barney J Venables ◽  
Ratnesh Chaturvedi ◽  
...  
Keyword(s):  
Flowering Time ◽  
Systemic Acquired Resistance ◽  
Defense Mechanism ◽  
Acquired Resistance ◽  
Early Flowering ◽  
Negative Regulator ◽  
Flowering Locus T ◽  
Autonomous Pathway ◽  
Abietane Diterpenoids ◽  
Flowering Locus

Abstract Abietane diterpenoids are tricyclic diterpenes whose biological functions in angiosperms are largely unknown. Here, we show that dehydroabietinal (DA) fosters transition from the vegetative phase to reproductive development in Arabidopsis thaliana by promoting flowering time. DA’s promotion of flowering time was mediated through up-regulation of the autonomous pathway genes FLOWERING LOCUS D (FLD), RELATIVE OF EARLY FLOWERING 6 (REF6), and FVE, which repress expression of FLOWERING LOCUS C (FLC), a negative regulator of the key floral integrator FLOWERING LOCUS T (FT). Our results further indicate that FLD, REF6, and FVE are also required for systemic acquired resistance (SAR), an inducible defense mechanism that is also activated by DA. However, unlike flowering time, FT was not required for DA-induced SAR. Conversely, salicylic acid, which is essential for the manifestation of SAR, was not required for the DA-promoted flowering time. Thus, although the autonomous pathway genes FLD, REF6, and FVE are involved in SAR and flowering time, these biological processes are not interdependent. We suggest that SAR and flowering time signaling pathways bifurcate at a step downstream of FLD, REF6, and FVE, with an FLC-dependent arm controlling flowering time, and an FLC-independent pathway controlling SAR.

scholarly journals GWAS hints at pleiotropic roles for FLOWERING LOCUS T in flowering time and yield-related traits in canola

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Harsh Raman ◽  
Rosy Raman ◽  
Yu Qiu ◽  
Avilash Singh Yadav ◽  
Sridevi Sureshkumar ◽  
...  
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals Natural Variation of the RICE FLOWERING LOCUS T 1 Contributes to Flowering Time Divergence in Rice

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75959 ◽  
Author(s):  
Eri Ogiso-Tanaka ◽  
Kazuki Matsubara ◽  
Shin-ichi Yamamoto ◽  
Yasunori Nonoue ◽  
Jianzhong Wu ◽  
...  
Keyword(s):  
Flowering Time ◽  
Natural Variation ◽  
Flowering Locus T ◽  
Flowering Locus

Expression of a FLOWERING LOCUS T homologue is temporally associated with annual flower bud initiation in Eucalyptus globulus subsp. globulus (Myrtaceae)

2011 ◽  
Vol 59 (8) ◽  
pp. 756 ◽  
Author(s):  
Rebecca C. Jones ◽  
Valérie F. G. Hecht ◽  
Brad M. Potts ◽  
René E. Vaillancourt ◽  
James L. Weller
Keyword(s):  
Eucalyptus Globulus ◽  
Expression Patterns ◽  
Flower Initiation ◽  
Flowering Locus T ◽  
Flower Bud ◽  
Reproductive Growth ◽  
Environmental Signals ◽  
Flowering Genes ◽  
Flowering Locus ◽  
Bud Initiation

The transition to flowering in plants is the result of the balance of endogenous processes and environmental signals that act through a complex genetic pathway that has been studied extensively in annual plants such as Arabidopsis. Perennial trees are characterised by a juvenile non-flowering phase lasting several years followed by an adult phase in which there is repeated cycling between vegetative and reproductive growth. The genetic control of flowering time is potentially more complex in perennials than in annuals and is less understood. Here, we examine the control of flowering in Eucalyptus globulus subsp. globulus, an important forestry species in temperate parts of the world. The E. globulus subsp. globulus homologues of two important flowering genes FLOWERING LOCUS T (FT) and LEAFY (LFY) were isolated and quantitative RT-PCR was used to measure their expression over a 2-year period. The expression of the homologue of FT in E. globulus subsp. globulus leaves was associated with the annual transition from vegetative to reproductive growth (i.e. flower bud initiation). Expression of the LFY homologue was associated with early flower bud development. In a comparison of FT and LFY expression patterns in two clones each of an early and late anthesis genotype, no association between the expression of these genes and the timing of anthesis was shown. Taken together, this indicates that FT and LFY could form part of the flower initiation pathway in Eucalyptus but do not regulate the observed differences in anthesis time.

scholarly journals BBX19 Interacts with CONSTANS to Repress FLOWERING LOCUS T Transcription, Defining a Flowering Time Checkpoint in Arabidopsis

2014 ◽  
Vol 26 (9) ◽  
pp. 3589-3602 ◽  
Author(s):  
C.-Q. Wang ◽  
C. Guthrie ◽  
M. K. Sarmast ◽  
K. Dehesh
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals Evolution of the miR5200-FLOWERING LOCUS T flowering time regulon in the temperate grass subfamily Pooideae

2017 ◽  
Vol 114 ◽  
pp. 111-121 ◽  
Author(s):  
Meghan McKeown ◽  
Marian Schubert ◽  
Jill C. Preston ◽  
Siri Fjellheim
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus
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