Modification of In Vitro and In Situ Dry Matter Digestibility of Forages with Chemical Treatment

jpa ◽  
1990 ◽  
Vol 3 (1) ◽  
pp. 83-87 ◽  
Author(s):  
N. D. Turner ◽  
G. T. Schelling ◽  
L. W. Greene ◽  
F. M. Byers
Author(s):  
O. Carrillo- Muro ◽  
R.G. Ramírez- Lozano ◽  
P. Hernández- Briano ◽  
M.A. López- Carlos ◽  
M. Guerrero- Cervantes ◽  
...  

The objectives of this study were to determine the chemical composition and dry matter digestibility of Quercus resinosa leaves harvested at different seasons of the year and to assess the intake and palatability of lamb diets containing foliage of Q. resinosa. There were three sites of collection, two located in the state of Jalisco and one in the state of Zacatecas, México. The young leaves harvested in spring, had greatest (P >0.05) nutritional value than leaves harvested in other seasons. The in vitro digestibility of organic matter (IVOMD), rapidly degradable fraction (a) and the degradation rate (c) were greater (P >0.05) in leaves harvested in spring and winter. The slowly degradable fraction (b) was greater (P >0.05) in spring, summer and autumn, and lesser in winter. The potential degradation fraction (a + b) and the effective degradability of dry matter were maximized (P >0.05) in spring than in the other seasons. The consumption was similar (P >0.05) among diets with different inclusion levels of leaves, but sheep consumed greater (P >0.05) amounts of diet containing leaves collected in spring. In conclusion, the Q. resinosa foliage contains the nutritional value and the digestibility and palatability that makes it suitable to replace completely the forage in the fattening diets of sheep, being preferable to use leaves harvested in spring and winter.


1993 ◽  
Vol 73 (4) ◽  
pp. 993-996 ◽  
Author(s):  
P. S. Mir ◽  
Z. Mir ◽  
L. Townley-Smith

Mature fenugreek and alfalfa were harvested as hay and compared with alfalfa cut at 10% bloom on the basis of proximate and mineral composition, in vitro dry matter digestibility (IVDMD) and in situ degradation characteristics. IVDMD of fenugreek was 73.1%, which was the highest (P < 0.05). The dry matter degradation characteristics in the rumen of fenugreek and alfalfa cut at 10% bloom were similar. These results suggest that mature fenugreek hay was comparable in nutrient content and digestibility to early cut alfalfa. Key words: Fenugreek, alfalfa, in vitro digestibility, in situ rumen degradation, nylon bag


2005 ◽  
Vol 76 (3) ◽  
pp. 303-308
Author(s):  
Norio TOKITA ◽  
Hitoshi KARAAMA ◽  
Munenori NEKOOKI ◽  
Yoko YAMASATO ◽  
Masataka SHIMOJO ◽  
...  

2020 ◽  
Vol 8 (1) ◽  
pp. 264
Author(s):  
Fabio Seiji dos Santos ◽  
Alexandre Menezes Dias ◽  
Luís Carlos Vinhas Ítavo ◽  
Ulysses Cecato ◽  
Andressa Faccenda ◽  
...  

This study aimed to evaluate the nutritional value of legumes leucaena and desmodium preserved in hay and silage form. We used a completely randomized design in a 2x2 factorial arrangement with two bulky conservation methods (hay and silage) and two legumes (leucaena and desmodium). For the bulky characterization was determined chemical composition and total digestible nutrients (TDN). For assessing the nutritional value, analysis of in vitro dry matter digestibility (IVDMD) and cell wall digestibility (IVCWD), and in situ effective degradability of dry matter (EDDM) and crude protein (EDCP) were performed. Data were submitted to ANOVA and Tukey test at 5% probability. Due to the characteristics of each specie leucaena presented a higher crude protein (CP) and TDN content compared to desmodium and lower fibrous fractions. The IVDMD presented interaction between the conservation form and forage (P<0.05), where leucaena hay was higher than the others treatments. The IVCWD also presented interaction (P<0.05), and the best values were obtained for leucaena silage. EDDM was superior in leucaena compared to desmodium (P<0.05). Regarding the method of preservation, the EDDM was higher in hay compared to silage. For the EDCP there was an interaction between conservation method and forage, where leucaena silage and hay were similar (P<0.05), but higher than other treatments. Thus, leucaena has a nutritional value higher than desmodium and the conservation of legumes in the hay form presented a higher nutritional value to the conserved as silage.


2012 ◽  
Vol 52 (5) ◽  
pp. 338 ◽  
Author(s):  
Franco Tagliapietra ◽  
Mirko Cattani ◽  
Ida K. Hindrichsen ◽  
Hanne H. Hansen ◽  
Stefania Colombini ◽  
...  

The aim of the study was to determine the comparability of the true dry matter digestibility (TDMd) achieved in situ with either traditional nylon bags (IS nylon) or synthetic filter bags (IS filter), and in vitro with either conventional bottles (CB) or the DaisyII incubation technique, using rumen fluid collected by oro-ruminal suction from intact cows. The four TDMd results were compared by linear regression. For each method, 11 feeds were incubated for 48 h in two separate incubations. The reproducibility of TDMd measurements was 97.9%, 95.1%, 98.8% and 96.0% for IS nylon, IS filter, CB and DaisyII, respectively. The determination coefficient, the slope (b), and the significant difference of the slope from unity, of the linear relationship between TDMd values (g/kg DM) were as follows: IS nylon v. IS filter, R2 = 0.97, b = 0.86, P = 0.02; IS nylon v. CB, R2 = 0.90, b = 1.02, P = 0.27; IS nylon v. DaisyII, R2 = 0.90, b = 1.06, P < 0.01; and IS filter v. DaisyII, R2 = 0.95, b = 0.98, P = 0.13. In situ filter bags and DaisyII underestimated the TDMd values compared with IS nylon bags and CB, respectively. In situ, the replacement of nylon with filter bags could simplify the procedure of analysis, with less manipulation of residuals and less labour, and offers the possibility to increase the number of samples analysed simultaneously. The close relationships found among methods suggest that rumen fluid collected from intact cows can produce in vitro estimates of TDMd at 48 h well correlated with those obtained in situ.


1991 ◽  
Vol 71 (3) ◽  
pp. 773-780 ◽  
Author(s):  
J. J. McKinnon ◽  
J. A. Olubobokun ◽  
D. A. Christensen ◽  
R. D. H. Cohen

The influence of temperature (125 or 145 °C) and duration (10, 20 or 30 min) of heating, or chemical treatment (acetic (AA) or formic (FA) acid) on in vitro insoluble DM (IDM) and insoluble CP (ICP) and 12 h in situ ruminal disappearance of canola meal was examined. Ruminal disappearances of dry matter (DDM) and crude protein (CPD) were expressed as apparent (ADMD and ACPD), insoluble (IDMD and ICPD) and degradable (DDMD and DCPD). Insoluble DM content was reduced by AA and FA (P < 0.01), while ICP was reduced by FA (P < 0.01). Acid treatment had no effect on in situ DMD or CPD. Heating increased IDM and ICP (P < 0.01). Duration of heating increased ICP at 145 °C (P < 0.01). Apparent DMD was influenced by temperature (P < 0.01) but not duration of heating. Mean ADMD and ACPD for control, 125 and 145 °C were 57.8, 59.6; 36.8, 24.1; and 27.4%, 13.7%, respectively, (P < 0.01). Insoluble and degradable DMD and CPD were reduced by heating (P < 0.01) but the influence of temperature or duration of heating was not consistent. The results indicate that heating at 125 or 145 °C for 10 min or more will reduce in situ DM and CP disappearance of canola meal, and increase the post-ruminal supply of DM and CP. Key words: Canola meal, in vitro solubility, in situ digestibility, heat and acid treatment


Author(s):  
Janet H. Woodward ◽  
D. E. Akin

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.


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