Trabecular Meshwork Stem Cells Could Be Differentiated to Be Trabecular Meshwork Cells with Secretory Functions

2019 ◽  
Author(s):  
Ying Su ◽  
Xin Jiang ◽  
Elizabeth (Xiaomeng) Wang ◽  
Feng Wang ◽  
Ying Han
Keyword(s):  
Stem Cells ◽  
Trabecular Meshwork ◽  
Trabecular Meshwork Cells

scholarly journals Human Trabecular Meshwork Cells Exhibit Several Characteristics of, but Are Distinct from, Adipose-Derived Mesenchymal Stem Cells

2014 ◽  
Vol 30 (2-3) ◽  
pp. 254-266 ◽  
Author(s):  
Joshua T. Morgan ◽  
Joshua A. Wood ◽  
Naomi J. Walker ◽  
Vijay Krishna Raghunathan ◽  
Dori L. Borjesson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Trabecular Meshwork ◽  
Trabecular Meshwork Cells

Stem cells from trabecular meshwork cells can secrete extracellular matrix

2020 ◽  
Vol 523 (2) ◽  
pp. 522-526 ◽  
Author(s):  
Elizabeth (Xiaomeng) Wang ◽  
Xin Jiang
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Trabecular Meshwork ◽  
Trabecular Meshwork Cells

scholarly journals Replacement of the Trabecular Meshwork Cells—A Way Ahead in IOP Control?

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1371
Author(s):  
Xiaochen Fan ◽  
Emine K. Bilir ◽  
Olivia A. Kingston ◽  
Rachel A. Oldershaw ◽  
Victoria R. Kearns ◽  
...  
Keyword(s):  
Stem Cells ◽  
Trabecular Meshwork ◽  
Vision Loss ◽  
Ex Vivo ◽  
Open Angle Glaucoma ◽  
Model Systems ◽  
Ex Vivo Model ◽  
Cell Replacement ◽  
Trabecular Meshwork Cells ◽  
Stem Cell Source

Glaucoma is one of the leading causes of vision loss worldwide, characterised with irreversible optic nerve damage and progressive vision loss. Primary open-angle glaucoma (POAG) is a subset of glaucoma, characterised by normal anterior chamber angle and raised intraocular pressure (IOP). Reducing IOP is the main modifiable factor in the treatment of POAG, and the trabecular meshwork (TM) is the primary site of aqueous humour outflow (AH) and the resistance to outflow. The structure and the composition of the TM are key to its function in regulating AH outflow. Dysfunction and loss of the TM cells found in the natural ageing process and more so in POAG can cause abnormal extracellular matrix (ECM) accumulation, increased TM stiffness, and increased IOP. Therefore, repair or regeneration of TM’s structure and function is considered as a potential treatment for POAG. Cell transplantation is an attractive option to repopulate the TM cells in POAG, but to develop a cell replacement approach, various challenges are still to be addressed. The choice of cell replacement covers autologous or allogenic approaches, which led to investigations into TM progenitor cells, induced pluripotent stem cells (iPSCs), and mesenchymal stem cells (MSCs) as potential stem cell source candidates. However, the potential plasticity and the lack of definitive cell markers for the progenitor and the TM cell population compound the biological challenge. Morphological and differential gene expression of TM cells located within different regions of the TM may give rise to different cell replacement or regenerative approaches. As such, this review describes the different approaches taken to date investigating different cell sources and their differing cell isolation and differentiation methodologies. In addition, we highlighted how these approaches were evaluated in different animal and ex vivo model systems and the potential of these methods in future POAG treatment.

scholarly journals Induction of Trabecular Meshwork Cells From Induced Pluripotent Stem Cells

2014 ◽  
Vol 55 (11) ◽  
pp. 7065 ◽  
Author(s):  
Qiong J. Ding ◽  
Wei Zhu ◽  
Amy C. Cook ◽  
Kristin R. Anfinson ◽  
Budd A. Tucker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Trabecular Meshwork ◽  
Pluripotent Stem Cells ◽  
Trabecular Meshwork Cells ◽  
Induced Pluripotent

scholarly journals Two-step induction of trabecular meshwork cells from induced pluripotent stem cells for glaucoma

2020 ◽  
Vol 529 (2) ◽  
pp. 411-417 ◽  
Author(s):  
Ajay Kumar ◽  
Tianyu Cheng ◽  
Weitao Song ◽  
Brandon Cheuk ◽  
Enzhi Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Trabecular Meshwork ◽  
Pluripotent Stem Cells ◽  
Trabecular Meshwork Cells ◽  
Induced Pluripotent

Mechanical stretch upregulates connexin43 in human trabecular meshwork cells

2019 ◽  
Author(s):  
Nikoleta Tellios ◽  
Mary Feng ◽  
Nancy Chen ◽  
Hong Liu ◽  
Vasiliki Tellios ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Mechanical Stretch ◽  
Trabecular Meshwork Cells

Effect of dexamethasone on the expression of MMPs, adenosine A1 receptors and NFKB by human trabecular meshwork cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Normie Aida Mohd Nasir ◽  
Renu Agarwal ◽  
Anna Krasilnikova ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Igor Iezhitsa
Keyword(s):  
Extracellular Matrix ◽  
Western Blot ◽  
Trabecular Meshwork ◽  
Culture Media ◽  
Smooth Muscle Actin ◽  
Adenosine A1 Receptors ◽  
Trabecular Meshwork Cells ◽  
Aqueous Humor Dynamics ◽  
Nfkb Activation ◽  
Adenosine A1

AbstractObjectivesSteroid-induced ocular hypertension and glaucoma are associated with extracellular matrix remodeling at the trabecular meshwork (TM) of the eye due to reduced secretion of matrix metalloproteinases (MMPs), a family of enzymes regulating extracellular matrix proteolysis. Several biological functions of steroids are known to involve regulation of adenosine A1 receptors (A1AR) and nuclear factor kappa B (NFKB). Since MMPs expression in TM has been shown to be regulated by A1AR as well as transcription factors, it is likely that dexamethasone-induced changes in aqueous humor dynamics involve reduced MMP and A1AR expression and reduced NFKB activation. Hence, the current study investigated the association of dexamethasone-induced reduction in MMP secretion with reduced NFKB activation and A1AR expression.MethodsHuman trabecular meshwork cells (HTMCs) were characterized by estimating myocilin and alpha smooth muscle actin expression and then were treated with dexamethasone 100 nM for 2, 5 and 7 days. The MMP secretion was estimated in culture media using Western blot. Immunocytochemistry (ICC) and ELISA were done to investigate the effect of dexamethasone on NFKB phosphorylation. A1AR expression in HTMCs was determined using Western blot and ELISA.ResultsDexamethasone caused a significant reduction in both MMP-2 and -9 expression compared to untreated group after five and seven days but not after two days of culture. Significantly reduced phosphorylated NFKB and A1AR protein levels were detected in dexamethasone treated compared to vehicle treated HTMCs after five days of culture.ConclusionsDexamethasone reduces MMP-2 and -9 secretion by HTMCs and this effect of dexamethasone is associated with reduced NFKB phosphorylation and A1AR expression.

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