scholarly journals Regulating DSC2 Expression Affects the Proliferation and Apoptosis of Prostate Cancer Cells

2020 ◽  
Vol Volume 12 ◽  
pp. 11453-11462
Author(s):  
Feng Jiang ◽  
Pengfei Wu
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Proliferation And Apoptosis

scholarly journals Cell Cycle-Dependent Effects of 3,3′-Diindolylmethane on Proliferation and Apoptosis of Prostate Cancer Cells

2009 ◽  
Vol 219 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Kannagi Chinnakannu ◽  
Di Chen ◽  
Yiwei Li ◽  
Zhiwei Wang ◽  
Q. Ping Dou ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Proliferation And Apoptosis ◽  
Cycle Dependent

LncRNA PART1 modulates toll-like receptor pathways to influence cell proliferation and apoptosis in prostate cancer cells

2018 ◽  
Vol 399 (4) ◽  
pp. 387-395 ◽  
Author(s):  
Ming Sun ◽  
Donghua Geng ◽  
Shuqiang Li ◽  
Zhaofu Chen ◽  
Wenyan Zhao
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Tunel Staining ◽  
Toll Like Receptor ◽  
Prostate Cancer Cells ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Qrt Pcr ◽  
Proliferation And Apoptosis

AbstractWe investigated thoroughly the effect of lncRNA PART1 on prostate cancer cells proliferation and apoptosis, through regulating toll-like receptor (TLR) pathways. LncRNA PART1 expression was also examined by quantitative real-time polymerase chain reactions (qRT-PCR) in human tissues and the cells lines LNCaP and PC3. After transfection with si-PART1 or control constructs, the cell viability was measured by MTS and colony formation assays. In addition, the apoptosis rate of the prostate cancer cells was validated by TUNEL staining. Relationships between lncRNA PART1 expression and TLR pathway genes were demonstrated by qRT-PCR and Western blotting. High levels of lncRNA PART1 expression were correlated with advanced cancer stage and predication of poor survival. LncRNA PART1 levels was increased in PCa cells treated with 5α-dihydrotestosterone (DHT), confirming PART1 was directly induced by androgen. Moreover, down-regulation of lncRNA PART1 inhibited prostate cancer cell proliferation and accelerated cell apoptosis. In addition, lncRNA PART1 induced downstream genes expression in TLR pathways includingTLR3,TNFSF10andCXCL13to further influence prostate cancer cells, indicating its carcinogenesis on prostate cancer. LncRNA PART1 promoted cell proliferation ability and apoptosis via the inhibition of TLR pathways in prostate cancer. LncRNA PART1 could hence be considered as a new target in the treatment of prostate cancer.

The effect of curcumin on proliferation and apoptosis in LNCaP prostate cancer cells

2006 ◽  
Vol 3 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Lei Yang ◽  
Lijun Chen ◽  
Bin Meng ◽  
Jiangrui Suo ◽  
Hongmin Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Proliferation And Apoptosis

scholarly journals Regulation of circGOLPH3 and its binding protein CBX7 on the proliferation and apoptosis of prostate cancer cells

2020 ◽  
Vol 40 (12) ◽  
Author(s):  
Lifeng Gong ◽  
Yu Tang ◽  
Li Jiang ◽  
Wei Tang ◽  
Shengjun Luo
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Rna Binding ◽  
Prostate Cancer Cell ◽  
Binding Protein ◽  
Cancer Cell Proliferation ◽  
Proliferative Capacity ◽  
Prostate Cancer Cells ◽  
Proliferation And Apoptosis ◽  
Cellular Apoptosis

Abstract To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and flow cytometry. Binding proteins of CircGOLPH3 were identified by RNA pull-down, mass spectrometry, and RNA-binding protein immunoprecipitation (RIP) assays. The expressions of CircGOLPH3 and CBX7 were measured by qRT-PCR. The results showed that after overexpression of circGOLPH3, the proliferative capacity and the viability of PC-3cells were significantly improved, whereas apoptosis was inhibited. CircGOLPH3 could bind to the CBX7 protein that was highly expressed in the PC-3 cell. Additionally, a functional test on CBX7 showed that the CBX7 overexpression notably improved the proliferative capacity and the viability of PC-3 cells and decreased cellular apoptosis, which was consistent with the effects of circGOLPH3. The validated the present study that circGOLPH3 and its binding protein CBX7 can promote prostate cancer cell proliferation and inhibit apoptosis.

scholarly journals Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

2019 ◽  
Vol 18 ◽  
pp. 153303381988686 ◽  
Author(s):  
Yuan Yuan ◽  
Xiaogang Chen ◽  
Enying Huang
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Western Blot ◽  
Cancer Cells ◽  
Cell Apoptosis ◽  
Circular Rna ◽  
Micro Rna ◽  
Prostate Cancer Cells ◽  
Ubiquitin Protein Ligase ◽  
Proliferation And Apoptosis

Objective: This study aimed to investigate the effect of circular RNA itchy E3 ubiquitin protein ligase on cell proliferation and apoptosis and to explore its target micro-RNAs in prostate cancer cells. Methods: Circular RNA itchy E3 ubiquitin protein ligase expression in human prostate cancer cells and normal prostate epithelial cells was determined by real time-quantitative polymerase chain reaction assay. Circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids (circular RNA itchy E3 ubiquitin protein ligase(+) group and control overexpression plasmids group were transfected with PC-3 cells. Rescue experiment was performed by transfection of circular RNA itchy E3 ubiquitin protein ligase overexpression and micro-197 overexpression plasmids (circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids/micro RNA (+) group) into PC-3 cells. Cell Counting Kit-8 and annexin V/propidium iodide assays were conducted to evaluate cell proliferation and apoptosis, respectively. Western blot was performed to determine the expressions of apoptotic-related markers. Results: Circular RNA itchy E3 ubiquitin protein ligase expression was decreased in DU 145, 22RV1, VCaP, and PC-3 cells compared to RWPE cells. In PC-3 cells, cell proliferation rate was reduced in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group compared to control overexpression plasmids group at 48 hours and 72 hours. Cell apoptosis rate was elevated in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group compared to control overexpression plasmids group at 48 hours, and Western blot showed the similar results. Micro RNA-197 but not micro RNA-31 or micro RNA-432 was the target micro-RNA of circular RNA itchy E3 ubiquitin protein ligase. In rescue experiments, cell proliferation rate was elevated, but apoptosis rate was reduced in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids/micro RNA (+) group compared to circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group, indicating that circular RNA itchy E3 ubiquitin protein ligase upregulation inhibited cell proliferation but promoted apoptosis through downregulating micro RNA-197. Conclusion: Circular RNA itchy E3 ubiquitin protein ligase upregulation suppresses cell proliferation but promotes apoptosis through targeting micro RNA-197 in prostate cancer. Our study may provide a new insight for the treatment of prostate cancer.

scholarly journals Analysis of Transcriptome, Selected Intracellular Signaling Pathways, Proliferation and Apoptosis of LNCaP Cells Exposed to High Leptin Concentrations

2019 ◽  
Vol 20 (21) ◽  
pp. 5412 ◽  
Author(s):  
Marta Szyszka ◽  
Lukasz Paschke ◽  
Marianna Tyczewska ◽  
Karol Jopek ◽  
Piotr Celichowski ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Signaling Pathways ◽  
Intracellular Signaling ◽  
Gene Set Enrichment Analysis ◽  
Mitogen Activated Protein Kinase ◽  
Lncap Cells ◽  
Prostate Cancer Cells ◽  
Inhibition Of Proliferation ◽  
Proliferation And Apoptosis

Leptin, the first discovered adipokine, has been connected to various physiological and pathophysiological processes, including cancerogenesis. Increasing evidence confirms its influence on prostate cancer cells. However, studies on the effects of leptin on the proliferation and apoptosis of the androgen-sensitive LNCaP line of prostate cancer cells brought conflicting results. Therefore, we performed studies on the effects of high LEP concentration (1 × 10−6 M) on gene expression profile, change of selected signaling pathways, proliferation and apoptosis of LNCaP cells. RTCA (real-time cell analyzer) revealed inhibitory effect of LEP on cell proliferation, but lower LEP concentrations (10−8 and 10−10 M) did not affect cell division. Moreover, flow cytometry with a specific antibody for Cleaved PARP-1, an apoptosis marker, confirmed the activation of apoptosis in leptin-exposed LNCaP line of prostate cancer cells. Within 24 h LEP (10−6 M) increases expression of 297 genes and decreases expression of 119 genes. Differentially expressed genes (DEGs) were subjected to functional annotation and clusterization using the DAVID bioinformatics tools. Most ontological groups are associated with proliferation and apoptosis (seven groups), immune response (six) and extracellular matrix (two). These results were confirmed by the Gene Set Enrichment Analysis (GSEA). The leptin’s effect on apoptosis stimulation was also confirmed using Pathview library. These results were also confirmed by qPCR method. The results of Western Blot analysis (exposure to LEP 10 min, 1, 2, 4 and 24 h) suggest (after 24 h) decrease of p38 MAPK, p44-42 mitogen-activated protein kinase and Bcl-2 phosphorylated at threonine 56. Moreover, exposure of LNCaP cells to LEP significantly stimulates the secretion of matrix metallopeptidase 7 (MMP7). Obtained results suggest activation of apoptotic processes in LNCaP cells cultured at high LEP concentration. At the same time, this activation is accompanied by inhibition of proliferation of the tested cells.

Role of glyoxalase I in the proliferation and apoptosis control of human LNCaP and PC3 prostate cancer cells

The Prostate ◽  
2012 ◽  
Vol 73 (2) ◽  
pp. 121-132 ◽  
Author(s):  
Cinzia Antognelli ◽  
Letizia Mezzasoma ◽  
Katia Fettucciari ◽  
Ettore Mearini ◽  
Vincenzo N. Talesa
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Glyoxalase I ◽  
Prostate Cancer Cells ◽  
Proliferation And Apoptosis
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