<p>The Effects of Synbiotic Supplementation on Glycemic Status, Lipid Profile, and Biomarkers of Oxidative Stress in Type 1 Diabetic Patients. A Placebo-Controlled, Double-Blind, Randomized Clinical Trial</p>

Type 1 diabetes (T1DM) is well recognized risk factor cardiovascular disease (CVD). Insulin therapy is recommended for all patients with type 1 diabetes. Previous findings showed that diabetes impairs endothelial function and increased glucose level reduces nitric oxide (NO) output and increases myeloperoxidase (MPO) activity. However, adiponectin (APN) decreases serum glucose levels. The current study evaluated effects of insulin therapy on circulating levels of oxidative stress and CVD biomarkers like NO, APN, MPO, AIP and lipid profile in type 1 diabetic patients. Fifty patients with T1DM and 18 healthy people were enrolled in this study. The recruited people with T1DM were classified into two groups: 22 newly diagnosed (untreated) type 1 diabetic patients and 28 insulin treated patients. In all groups, circulating NO, APN, MPO, AIP and lipids levels were measured. Compared to control, untreated diabetes revealed a significant increase in the serum levels of APN, MPO, TG, VLDL, TC, LDL and AIP, with a marked reduction in NO and HDL levels. However, insulin therapy significantly lowered MPO, TC and LDL, with no significant changes in the other biochemical parameters. As expected, oxidative stress and CVD-associated markers were significantly increased in untreated diabetes. Insulin therapy exhibited a relatively positive effect on oxidative stress and CVD biomarkers. Accordingly, insulin plus antioxidant supplementation required to normalize these parameters.

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Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes

Journal of Clinical Medicine ◽

10.3390/jcm9072155 ◽

2020 ◽

Vol 9 (7) ◽

pp. 2155

Author(s):

Francesca Iannantuoni ◽

Aranzazu M. de Marañon ◽

Zaida Abad-Jiménez ◽

Francisco Canet ◽

Pedro Díaz-Pozo ◽

...

Keyword(s):

Oxidative Stress ◽

Type 1 Diabetes ◽

Adhesion Molecules ◽

Mitochondrial Function ◽

Mitochondrial Membrane ◽

Diabetic Patients ◽

Ros Production ◽

Mitochondrial Ros ◽

Type 1 Diabetic Patients

Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte–endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNFα) and myeloperoxidase (MPO). HbA1C and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte–endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNFα and MPO, and a decreased expression of SOD. The enhancement of leukocyte–endothelium interactions and proinflammatory markers correlated with glucose and HbA1Clevels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte–endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications.

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Treatment of Recent-Onset Type 1 Diabetic Patients With DiaPep277: Results of a Double-Blind, Placebo-Controlled, Randomized Phase 3 Trial. Diabetes Care 2014;37:1392–1400. DOI: 10.2337/dc13-1391

Diabetes Care ◽

10.2337/dc15-rt01 ◽

2014 ◽

Vol 38 (1) ◽

pp. 178-178 ◽

Cited By ~ 1

Author(s):

Itamar Raz ◽

Anette G. Ziegler ◽

Thomas Linn ◽

Guntram Schernthaner ◽

Francois Bonnici ◽

...

Keyword(s):

Diabetes Care ◽

Diabetic Patients ◽

Phase 3 ◽

Double Blind ◽

Double Blind Placebo ◽

Recent Onset ◽

Onset Type ◽

Type 1 Diabetic Patients

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Effect of multivitamin versus multivitamin-mineral supplementation on metabolic profiles and biomarkers of oxidative stress in pregnant women: a double-blind randomized clinical trial

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.3109/14767058.2014.954241 ◽

2014 ◽

Vol 28 (11) ◽

pp. 1336-1342 ◽

Cited By ~ 2

Author(s):

Mohsen Taghizadeh ◽

Mansooreh Samimi ◽

Fariba Kolahdooz ◽

Zohreh Tabassi ◽

Mehri Jamilian ◽

...

Keyword(s):

Oxidative Stress ◽

Clinical Trial ◽

Pregnant Women ◽

Randomized Clinical Trial ◽

Metabolic Profiles ◽

Double Blind ◽

Mineral Supplementation ◽

Biomarkers Of Oxidative Stress

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Oxidative stress in families of type 1 diabetic patients

Atherosclerosis ◽

10.1016/s0021-9150(00)80068-4 ◽

2000 ◽

Vol 151 (1) ◽

pp. 15-16

Author(s):

E Matteucci ◽

V Cinapri ◽

S Quilici ◽

G Forotti ◽

O Giampietro

Keyword(s):

Oxidative Stress ◽

Diabetic Patients ◽

Type 1 Diabetic Patients

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Effects of L-carnitine supplementation on biomarkers of oxidative stress, antioxidant capacity and lipid profile, in patients with pemphigus vulgaris: a randomized, double-blind, placebo-controlled trial

European Journal of Clinical Nutrition ◽

10.1038/ejcn.2017.131 ◽

2017 ◽

Vol 72 (1) ◽

pp. 99-104 ◽

Cited By ~ 8

Author(s):

H Mohammadi ◽

M Djalali ◽

M Daneshpazhooh ◽

N M Honarvar ◽

C Chams‐Davatchi ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Lipid Profile ◽

Controlled Trial ◽

Pemphigus Vulgaris ◽

Carnitine Supplementation ◽

Double Blind ◽

Double Blind Placebo ◽

Biomarkers Of Oxidative Stress

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Impact of flexible insulin therapy on blood glucose variability, oxidative stress and inflammation in type 1 diabetic patients: The VARIAFIT study

Diabetes & Metabolism ◽

10.1016/j.diabet.2014.01.004 ◽

2014 ◽

Vol 40 (4) ◽

pp. 278-283 ◽

Cited By ~ 2

Author(s):

P.Y. Benhamou ◽

F. Somers ◽

S. Lablanche ◽

I. Debaty ◽

A.L. Borel ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Glucose ◽

Insulin Therapy ◽

Glucose Variability ◽

Diabetic Patients ◽

Blood Glucose Variability ◽

Type 1 Diabetic Patients

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Follow Up of Value of the Intrarenal Resistivity Indices and Different Renal Biomarkers for Early Identification of Diabetic Nephropathy in Type 1 Diabetic Patients

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.034 ◽

2017 ◽

Vol 5 (2) ◽

pp. 188-192 ◽

Cited By ~ 1

Author(s):

Soha M. Abd El Dayem ◽

Abo El Magd El Bohy ◽

Mona Hamed ◽

Solaf Ahmed

Keyword(s):

Diabetic Nephropathy ◽

Lipid Profile ◽

Diabetic Patients ◽

Creatinine Ratio ◽

Albumin Creatinine Ratio ◽

Resistivity Index ◽

Type 1 Diabetic Patients ◽

Over Time

AIM: To evaluate intrarenal resistivity index (RI) and different biomarkers of diabetic nephropathy (DN) with clinical signs of DN and its progression over time as early detection of DN.PATIENTS AND METHODS: This longitudinal study included 48 type 1 diabetic patients who were studied at baseline and after three years. A blood sample was taken for assessment of glycosylated haemoglobin (HbA1), lipid profile and a urine sample was taken for assessment of albumin/creatinine ratio, Neutrophil gelatinase-associated lipocalin (NGAL), liver-type fatty acid binding protein (L-FABP) and kidney injury molecule-1 (Kim-1) at baseline and after three years. Forty diabetic patients did renal Doppler at baseline & after three years.RESULTS: HbA1, waist/hip ratio, albumin/creatinine ratio, lipid profile, NGAL, KIM-1, L-FABP and resistivity index (RI) were significantly increased in follow-up. Twenty patients (41.7%) showed progression to albuminuria. RI showed a significant increase in follow-up study. ROC curve showed that RI and NGAL had the highest sensitivity (100%), followed by L-FABP (90%) and lastly KIM-1 (63.6%) in the prediction of DN.CONCLUSION: High RI, NGAL, KIM-1 & L-FABP can be considered as early markers of diabetic nephropathy in type 1 diabetics and are associated with its progression over time, independent of albuminuria.

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