scholarly journals Nanostructured self-assembling peptides as a defined extracellular matrix for long-term functional maintenance of primary hepatocytes in a bioartificial liver modular device

2013 ◽  
pp. 1525 ◽  
Author(s):  
Shibashish Giri ◽  
Braumann ◽  
Giri ◽  
Acikgöz ◽  
Scheibe ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bioartificial Liver ◽  
Primary Hepatocytes ◽  
Self Assembling

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

2021 ◽  
pp. 039139882110089
Author(s):  
Lara Milian ◽  
María Sancho-Tello ◽  
Joan Roig-Soriano ◽  
Giovanna Foschini ◽  
Néstor J Martínez-Hernández ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Histological Study ◽  
Biomechanical Properties ◽  
Elastic Fibers ◽  
Appreciable Effect ◽  
Long Term Effects ◽  
Minimal Impact ◽  
Biomechanical Effect ◽  
Triton X

Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

Construct Sturdy Packing of Self‐Assembling Iridium Complex via Endocytic Trafficking for Long‐Term Lysosome Tracking

2021 ◽  
Author(s):  
Chengzhi Jin ◽  
Guanying Li ◽  
Xia Wu ◽  
Jiangpin Liu ◽  
Weijun Wu ◽  
...  
Keyword(s):  
Endocytic Trafficking ◽  
Iridium Complex ◽  
Self Assembling

scholarly journals The transcriptional landscape of a hepatoma cell line grown on scaffolds of extracellular matrix proteins

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Souvik Ghosh ◽  
Anastasiya Börsch ◽  
Shreemoyee Ghosh ◽  
Mihaela Zavolan
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Targets ◽  
Hepatoma Cell ◽  
Growth Potential ◽  
Collagen I ◽  
Matrix Proteins ◽  
Hepatoma Cell Line ◽  
Primary Hepatocytes

Abstract Background The behavior of cells in vivo is complex and highly dynamic, as it results from an interplay between intercellular matrix proteins with surface receptors and other microenvironmental cues. Although the effects of the cellular niche have been investigated for a number of cell types using different molecular approaches, comprehensive assessments of how the global transcriptome responds to 3D scaffolds composed of various extracellular matrix (ECM) constituents at different concentrations are still lacking. Results In this study, we explored the effects of two diverse extracellular matrix (ECM) components, Collagen I and Matrigel, on the transcriptional profile of cells in a cell culture system. Culturing Huh-7 cells on traditional cell culture plates (Control) or on the ECM components at different concentrations to modulate microenvironment properties, we have generated transcriptomics data that may be further explored to understand the differentiation and growth potential of this cell type for the development of 3D cultures. Our analysis infers transcription factors that are most responsible for the transcriptome response to the extracellular cues. Conclusion Our data indicates that the Collagen I substrate induces a robust transcriptional response in the Huh-7 cells, distinct from that induced by Matrigel. Enhanced hepatocyte markers (ALB and miR-122) reveal a potentially robust remodelling towards primary hepatocytes. Our results aid in defining the appropriate culture and transcription pathways while using hepatoma cell lines. As systems mimicking the in vivo structure and function of liver cells are still being developed, our study could potentially circumvent bottlenecks of limited availability of primary hepatocytes for preclinical studies of drug targets.

SAT-188-Expansion and genetic modification of primary hepatocytes to study long-term hepatitis B virus infection

2019 ◽  
Vol 70 (1) ◽  
pp. e712
Author(s):  
Eleftherios Michailidis ◽  
Paul Park ◽  
Mohammad Kabbani ◽  
Liliana Mancio Silva ◽  
Yingpu Yu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Genetic Modification ◽  
Hepatitis B Virus Infection ◽  
Primary Hepatocytes ◽  
B Virus

ORGANIZATION OF EXTRACELLULAR MATRIX COMPONENTS DURING DIFFERENTIATION OF ADIPOCYTES IN LONG-TERM CULTURE

2000 ◽  
Vol 36 (1) ◽  
pp. 38 ◽  
Author(s):  
YASUAKI KUBO ◽  
SACHIKO KAIDZU ◽  
IKUYO NAKAJIMA ◽  
KAZUAKI TAKENOUCHI ◽  
FUMIO NAKAMURA
Keyword(s):  
Extracellular Matrix ◽  
Long Term Culture ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Proteoglycans in human long-term bone marrow cultures: biochemical and ultrastructural analyses

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1333-1343 ◽  
Author(s):  
TN Wight ◽  
MG Kinsella ◽  
A Keating ◽  
JW Singer
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cell Layer ◽  
Ruthenium Red ◽  
Adherent Cells ◽  
Sulfate Proteoglycan ◽  
Chondroitinase Abc ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

Proteoglycans within the extracellular matrix of human bone marrow have been implicated in the process of hematopoiesis, but little is known about the structure and composition of these macromolecules in this tissue. Hematopoietically active human long-term bone marrow cultures were incubated with medium containing 35S-sulfate and 3H-glucosamine as labeling precursors. Proteoglycans present in the medium and cell layer were extracted with 4 mol/L guanidine HCI and purified by diethylaminoethyl (DEAE)-Sephacel ion exchange and molecular sieve chromatography. Both culture compartments contain a large chondroitin sulfate proteoglycan (MI, CI) that eluted in the void volume of a Sepharose CL-4B column and contained glycosaminoglycan chains of molecular weight (mol wt) approximately 38,000. A second population of sulfate-labeled material was identified as a broad heterogenous peak (MII, CII) that was included on Sepharose CL-4B at Kav = 0.31. This material when chromatographed on Sepharose CL-6B could be further separated into a void peak (MIIa, CIIa) and an included peak eluting at Kav = 0.39 (MIIb, CIIb). The void peaks (MIIa, CIIa) were susceptible to chondroitinase ABC digestion (99%) but slightly less susceptible to chondroitinase AC digestion (90%). Papain digestion of these peaks revealed them to be proteoglycans with glycosaminoglycan chains of mol wt approximately 38,000. The included peaks on Sepharose CL-6B (MIIb, CIIb) from both medium and cell layer compartments resisted digestion with papain, indicating the presence of glycosaminoglycan chains of mol wt approximately 38,000 either free or attached to a small peptide. Although this material was susceptible to chondroitinase ABC (98%), it was considerably less susceptible to chondrotinase AC (approximately 60%), indicating that it contained dermatan sulfate. A small amount of heparan sulfate proteoglycan was also identified but constituted only approximately 10% of the total sulfated proteoglycan extracted from these cultures. Additionally, approximately 40% of the incorporated 3H- activity radioactivity was present as hyaluronic acid. Electron microscopy revealed a layer of adherent cells covered by a mat containing ruthenium red-positive granules that were connected by thin filaments. The extracellular matrix layer above the adherent cells contained a mixture of hematopoietic cells. Chondroitinase ABC treatment of the cultures completely removed the ruthenium red-positive granules overlying the cells and resulted in a loss of approximately 70% of the 35S-sulfate-labeled material from the cell layer.(ABSTRACT TRUNCATED AT 400 WORDS)

High long-term survival and function of cryopreserved primary hepatocytes in two different culture systems

2003 ◽  
Vol 124 (4) ◽  
pp. A736
Author(s):  
Meindert Sosef ◽  
Keishi Sugimachi ◽  
John Baust ◽  
Mehmet Toner
Keyword(s):  
Primary Hepatocytes ◽  
Term Survival ◽  
Long Term Survival ◽  
Culture Systems ◽  
And Function

Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes

Materialia ◽  
2018 ◽  
Vol 4 ◽  
pp. 518-528 ◽  
Author(s):  
Ronald Bual ◽  
Haruna Kimura ◽  
Yasuhiro Ikegami ◽  
Nana Shirakigawa ◽  
Hiroyuki Ijima
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Culture ◽  
Functional Evaluation ◽  
Primary Hepatocytes

scholarly journals Printability Optimization of Gelatin-Alginate Bioinks by Cellulose Nanofiber Modification for Potential Meniscus Bioprinting

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Wenbin Luo ◽  
Zhengyi Song ◽  
Zhonghan Wang ◽  
Zhenguo Wang ◽  
Zuhao Li ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Knee Joint ◽  
Cellulose Nanofiber ◽  
Cellular Viability ◽  
Meniscal Injury ◽  
Biological Tests ◽  
Biological Environment ◽  
Matrix Accumulation

Meniscal injury is more likely to cause a permanent alteration of the biomechanical and biological environment of the knee joint, mainly due to the morphological mismatch and substantial loss of meniscal tissues. Herein, to overcome this challenge, we developed an improved bioink with enhanced printability, while maintaining the biocompatibility of major cellular component of the meniscus, namely fibrochondrocytes. Firstly, cellulose nanofiber (CNF) was mixed with gelatin-alginate thermal-responsive bioinks to improve the printability. Afterward, individual-specific meniscal prototypes based on the 3D reconstruction of MRI data were bioprinted using our bioink. The rheological and printability properties of the bioinks were characterized to select proper bioink content and bioprinting parameters. And then, a series of biological characterizations of the bioprinted samples, such as cell viability, metabolic activity, and extracellular matrix accumulation, were carried out in vitro. The results indicated that superior rheological performance and printability of CNF-modified bioink were achieved, ensuring high-precision bioprinting of specific-designed meniscal prototype when compared with the non-CNF-containing counterparts. Meanwhile, biological tests indicated that fibrochondrocytes encapsulated within the CNF-modified bioink maintained long-term cellular viability as well as acceptable extracellular matrix accumulation. This study demonstrates that the CNF-modified bioink is in favor of the printing fidelity of specific meniscus by improved rheological properties, minimizing the mismatch between artificial meniscal implants and native knee joint tissues, thereby permitting the evolution of clinical therapeutic methods of meniscal reconstruction.

scholarly journals Extracellular Matrix Protects Pancreatic  -Cells Against Apoptosis: Role of Short- and Long-Term Signaling Pathways

Diabetes ◽  
2004 ◽  
Vol 53 (8) ◽  
pp. 2034-2041 ◽  
Author(s):  
E. Hammar ◽  
G. Parnaud ◽  
D. Bosco ◽  
N. Perriraz ◽  
K. Maedler ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Signaling Pathways ◽  
Pancreatic Cells ◽  
Short And Long Term
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