scholarly journals Molecular characterization of new Buffelgrass (Cenchrus ciliaris) genotypes

Plant Omics ◽  
2020 ◽  
pp. 104-107
Author(s):  
Abdulrahman Al-Soqeer ◽  
Soleman M. Al-Otayk ◽  
Mohamed I. Motawei
Keyword(s):  
Genetic Variation ◽  
Arid Regions ◽  
Inter Simple Sequence Repeat ◽  
Cattle Production ◽  
Similarity Coefficients ◽  
Cenchrus Ciliaris ◽  
Local Populations ◽  
Maximum Similarity ◽  
Simple Sequence

Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats. Cenchrus ciliaris is apomictic, a condition that is normally associated with low genetic variation within populations, but even moderate levels of genetic variation among populations could account for differences in invasiveness. We used Inter-Simple Sequence Repeat markers (ISSRs) to examine genetic variation among buffelgrass genotypes collected from nine different locations in the central region of Saudi Arabia, and three introduced cultivars. The genetic similarity coefficients among the twelve buffelgrass genotypes ranged from 0.66 to 0.94. Maximum similarity was observed between local populations. Introduced cultivars 'Biloela', 'Gayndah' and 'USA' were quite distinct from local populations. The dendogram constructed with UPGMA analysis revealed three clusters. In general, the study revealed the presence of considerable genetic diversity that could be used for further analysis for specific traits of interest.

Genetic variability of Orobanche aegyptiaca infesting tobacco in Iran by Bayesian analysis

Biologia ◽  
2014 ◽  
Vol 69 (12) ◽  
Author(s):  
Samaneh Abedi ◽  
Reza Darvishzadeh ◽  
Iraj Bernousi ◽  
Babak Abdollahi Mandoulakani ◽  
Hamid Hatami Maleki ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Genetic Groups ◽  
Wide Range ◽  
Orobanche Aegyptiaca ◽  
Simple Sequence ◽  
Issr Primers

AbstractBroomrapes (Orobanche L.) are holoparasitic plants, parasitizing roots of a wide range of host plants. In this study, genetic polymorphism among 44 Orobanche aegyptiaca Pers. individuals collected from different regions of northwest Iran was investigated using inter-simple sequence repeat (ISSR) markers. Two hundred-sixty one discernible bands were amplified using 20 ISSR primers which 245 (94%) was polymorphic, indicating considerable genetic variation among the examined individuals. The number of polymorphic bands per primer ranged from 4 to 17, averaging 12.25. UPGMA clustering using Jaccard’s similarity coefficients revealed six main groups. Genetic similarity coefficients varied from 0.71 (between individuals 23 and 27) to 0.34 (between 13 and 30). A model-based Bayesian approach subdivided 38 out of 44 broomrape genotypes into 2 genetic groups and the remaining ones were categorized as mixed genotypes based on Q values. According to an analysis of molecular variance, 99% of the total variation was partitioned within genetic groups. The results demonstrated the potential usefulness of ISSR markers for determination of genetic variation in O. aegyptiaca. Understanding the variability in broomrape is important when attempting to develop resistant host crops against this parasite.

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

Characterization of the Russian beef cattle breed gene pools using inter simple sequence repeat DNA analysis (ISSR analysis)

2016 ◽  
Vol 52 (9) ◽  
pp. 963-968 ◽  
Author(s):  
G. E. Sulimova ◽  
V. N. Voronkova ◽  
A. V. Perchun ◽  
I. F. Gorlov ◽  
A. V. Randelin ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Dna Analysis ◽  
Cattle Breed ◽  
Inter Simple Sequence Repeat ◽  
Gene Pools ◽  
Issr Analysis ◽  
Repeat Dna ◽  
Simple Sequence ◽  
Simple Sequence Repeat Dna

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Determination of Genome Size, Chromosome Number, and Genetic Variation Using Inter-Simple Sequence Repeat Markers in Lotus spp.

CYTOLOGIA ◽  
2016 ◽  
Vol 81 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Hidenori Tanaka ◽  
Awatsaya Chotekajorn ◽  
Sayumi Kai ◽  
Genki Ishigaki ◽  
Masatsugu Hashiguchi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Chromosome Number ◽  
Genome Size ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence

Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

2008 ◽  
Vol 88 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Xiuliang Wang ◽  
Fengjuan Zhao ◽  
Zimin Hu ◽  
Alan T. Critchley ◽  
Steve L. Morrell ◽  
...  
Keyword(s):  
Genetic Variation ◽  
North Atlantic ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Chondrus Crispus ◽  
Sequence Repeat ◽  
Issr Analysis ◽  
Simple Sequence

scholarly journals Genetic variation of agarwood producing tree (Gyrinops versteegii) from Pongkor, Manggarai District, Flores Island, Indonesia using ISSR molecular markers

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
ALFI FAUZAN IRSYAD ◽  
Ridesti Rindyastuti ◽  
TITUT YULISTYARINI ◽  
AGUNG SRI DARMAYANTI ◽  
BUDI SETIADI DARYONO
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
Clustering Analysis ◽  
Fungal Infections ◽  
Inter Simple Sequence Repeat ◽  
Conservation Strategies ◽  
Species Identity ◽  
Issr Molecular Markers ◽  
Degree Of Similarity ◽  
Simple Sequence

Abstract. Irsyad AF, Rindyaastuti R, Yulistyarini T, Darmayanti AS, Daryono BS. 2020. Genetic variation of agarwood producing tree (Gyrinops versteegii) from Pongkor, Manggarai District, Flores Island, Indonesia using ISSR molecular markers. Biodiversitas 21: xxxx. Agarwood is a black-colored tree wood that produces distinctive sap because of fungal infections which belong to Thymelaeaceae family (mainly Aquilaria and Gyrinops). Agarwood product is highly valuable that leading to over exploitations by the collectors. To develop the most effective and efficient conservation strategies, genetic information from these plants is required. The aims of this research are to determine the genetic variation and to confirm the species identity of agarwood producing tree (Gyrinops versteegii (Gilg.) Domke) population in Pongkor Community Forest, Pongkor, Manggarai District, Flores Island, East Nusa Tenggara. Information of the genetic variation, as well as the phenetic relatedness, were evaluated with inter-simple sequence repeat molecular marker (ISSR) using five primers; Ng2.01, Ng2.06, Ng3.01, Ng3.02, and UBC 855, with two other agarwood producing species as outgroup (Aquilaria filaria and Gyrinops decipiens). Amplified bands from all primers showed 55.17% polymorphic bands in G. versteegii. Genetic variation of G. versteegii identified with Nei’s genetic diversity (h value) obtained at 0.218. Clustering analysis from UPGMA dendrograms formed three major clusters. Degree of similarity of G. versteegii based on the dendrograms obtained at 85.9% using SSM method. The results showed close phenetic relatedness between individuals and relatively high genetic variation of G. versteegii, however, imply the need for strictly maintenance of habitat preservation and larger population size.

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