scholarly journals Histological evaluation of effect of PDGF-rich plasma on the tympanic membrane

2020 ◽  
pp. 56-61
Author(s):  
A. I. Kriukov ◽  
A. V. Gurov ◽  
A. A. Bakhtin ◽  
Z. V. Murzakhanova
Keyword(s):  
Growth Factors ◽  
Connective Tissue ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Main Group ◽  
Histological Evaluation ◽  
Control Group ◽  
Membrane Perforation ◽  
Perforated Tympanic Membrane ◽  
Tympanic Membranes

Introduction. Тo study the microscopic picture of the tissues of the perforated tympanic membrane after the introduction of blood plasma enriched with platelet growth factors into the perforated area of the tympanic membrane in the experiment.Materials and methods. 36 rats (72 tympanic membranes) were examined. The animals were divided into 2 groups, the main and the control. The tympanic membrane was perforated in rats of both groups using sterile play. Animals of the main group were injected with a single application of autoplasm of blood enriched with platelet growth factors into the area of perforation of the tympanic membrane. The animals of the control group underwent only dynamic observation. The rats were withdrawn from the experiment in batches of 6 individuals on day 5, 10, 15 after the tympanic membrane perforation, then a histological assessment of the tissue regeneration of the tympanic membranes was performed. Results. In animals of the main group on the 5th day after perforation of the tympanic membrane, edema and disorganization of connective tissue were significantly less frequently recorded than in animals of the control group (p ≤ 0.05). Fibroblastic infiltration of tympanic membrane tissues was significantly more often observed in rats of the main group compared with rats in the control group (p ≤ 0.05). In rats of the main group on the 10th day after perforation of the tympanic membrane, edema and disorganization of the connective tissue of the tympanic membranes were noted significantly less frequently than in rats of the control group (p ≤ 0.05). Signs of fibroblastic infiltration and neoangiogenesis at the same time in animals of the main group were revealed significantly more often, in contrast to rats in the control group (p ≤ 0.05). In animals of the main group, on the 15th day after perforation of the tympanic membrane, fibroblastic infiltration and neoangiogenesis were revealed in all cases of observation, which significantly exceeded the corresponding indicators in the control group, and leukocyte infiltration in rats of the main group, in contrast to the previous stages of the study, was noted significantly less frequently. than in rats of the control group (p ≤ 0.05).Conclusion. Earlier and complete regeneration of damaged tissues of the tympanic membrane under conditions of its traumatic perforation was noted in rats of the main group under the influence of PCOTFR. In rats of the main group, more organized collagen fibers, the presence of basophilic leukocytes, signs of neoangiogenesis, and extensive fields of fibroblasts were visualized.

scholarly journals Eardrum perforation treatment blood plasma enriched with platelet growth factors

2021 ◽  
pp. 110-115
Author(s):  
A. I. Kriukov ◽  
A. V. Gurov ◽  
A. G. Ermolaev ◽  
Z. V. Murzakhanova ◽  
T. K. Dubovaya ◽  
...  
Keyword(s):  
Growth Factors ◽  
Blood Plasma ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Main Group ◽  
Control Group ◽  
Relative Area ◽  
Membrane Perforation ◽  
Post Traumatic

Introduction. The problem of the development of acute post-traumatic otitis media has not lost its relevance. Long-term preservation of the defect of the tympanic membrane contributes to the development of chronic inflammation in the middle ear and a deterioration in the quality of life of patients.Objective. To study the effect of blood plasma enriched with platelet growth factors on the regeneration of the tympanic membrane tissues in patients with acute post-traumatic perforation.Materials and methods. Patients with acute post-traumatic perforation of  the  tympanic membrane were divided into the main (24 people) and control (19 people) groups. Patients of the main group in the area of perforation of the tympanic membrane were injected once by application with a clot of autoplasma, enriched with platelet growth factors. The control group patients underwent dynamic observation of the processes of natural regeneration of the tympanic membrane tissues. On the 5, 10 and 15th days, the tissue regeneration of the tympanic membranes was assessed subjectively (patient complaints, audiometry) and objectively (otoscopy with the calculation of the dynamics of the relative perforation area, otoacoustic emission).Results. A single application of blood plasma enriched with platelet growth factors to the perforation area was significantly more often accompanied by closure of  the  tympanic membrane perforation (p ≤ 0.01) and a  decrease in  the  average relative area of the tympanic membrane perforation on days 10 and 15 of the study (p ≤ 0.01). The dynamics of the average relative area of the tympanic membrane perforation reflected a high rate of closure of the tympanic membrane defect in the main group. Better healing of the tympanic membrane in patients of the main group was accompanied by an improvement in the auditory function of the affected ear. The quality of hearing was, on average, significantly better in the patients of the main group than in the patients of the control group, both on the 10th and 15th days of observation (p ≤ 0.01).Conclusion. Clinical use of blood plasma enriched with platelet growth factors has a beneficial effect on the rate, intensity of closure of the tympanic membrane perforation and hearing. 

Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study

2008 ◽  
Vol 123 (5) ◽  
pp. 482-487 ◽  
Author(s):  
E Erkilet ◽  
M Koyuncu ◽  
S Atmaca ◽  
M Yarim
Keyword(s):  
Tympanic Membrane ◽  
Histopathological Examination ◽  
Platelet Rich Plasma ◽  
Healing Time ◽  
Tympanic Membrane Perforation ◽  
Control Group ◽  
Tympanic Membrane Perforations ◽  
Significant Difference ◽  
Membrane Perforation ◽  
Tympanic Membranes

AbstractObjective:The aim of this study was to investigate the effect of local application of platelet-rich plasma to perforated rat tympanic membranes, in terms of healing time and histopathological outcome.Methods:Eighty-eight tympanic membranes of 44 rats were given a standard 3 mm perforation, and platelet-rich plasma was applied to the right tympanic membrane perforations. The left tympanic membranes were left to heal spontaneously, as controls. The 44 rats were divided into two groups. In group one, comprising 20 rats, daily otomicroscopic examination of the tympanic membrane perforations was performed. The 24 rats in group two were subdivided into four subgroups of six rats each; these subgroups were sacrificed sequentially on days three, seven, 14 and 28 for histopathological examination, regardless of tympanic membrane healing stage.Results:In group one, the mean tympanic membrane healing times for tympanic membrane perforations receiving platelet-rich plasma and controls were respectively 10.2 ± 2.1 and 13.0 ± 2.9 days (mean ± standard deviation). This difference was statistically significant (p < 0.001). In group two, histopathological evaluation of tympanic membrane perforation healing at days three, seven, 14 and 28 did not reveal any statistically significant difference, individually or within the four groups as a whole.Conclusion:These findings suggest that earlier healing of tympanic membrane perforations occurred in the platelet-rich plasma group compared with the control group. These findings suggest that platelet-rich plasma is effective in accelerating tympanic membrane perforation healing, and that it may be effective in human subjects, particularly as it is an autologous material.

Effect of local irradiation with 630 and 860 nm low-level lasers on tympanic membrane perforation repair in guinea pigs

2013 ◽  
Vol 127 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Sh Maleki ◽  
S K Kamrava ◽  
D Sharifi ◽  
M Jalessi ◽  
A Asghari ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Guinea Pigs ◽  
Tympanic Membrane Perforation ◽  
Control Group ◽  
Histological Changes ◽  
Low Level ◽  
Combined Application ◽  
Membrane Perforation ◽  
Experimental Group ◽  
Tympanic Membranes

AbstractObjective:In this study, we evaluated the effect of low-level lasers on the healing of tympanic membrane perforation, one of the most common otological pathologies.Methods and materials:Twenty-four guinea pigs were randomly assigned to either the experimental or control group. One day after the induction of a 2 mm diameter, centred myringotomy in all animals, the tympanic membranes in the experimental group were irradiated with 630 and 860 nm lasers for 10 days. Two weeks later, histological changes in the membranes were evaluated.Results:Tympanic membrane thickening and inflammatory cell infiltration in the tympanic membranes and surrounding tissues were significantly less in the experimental group (p < 0.001). The distance from the external auditory canal wall to the malleus tip did not differ significantly between the two groups (p = 0.42).Conclusion:The results show that the combined application of 630 and 860 nm lasers had a significant effect on the healing of tympanic membrane perforation, and on the prevention of thick fibrotic or atelectatic neomembrane formation.

Middle Ear Packing: Comparison Between Merogel and Gelfoam

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P102-P102
Author(s):  
Michael Julian Lipan ◽  
Alava Ibraham ◽  
Simon I Angeli ◽  
Thomas R Van De Water
Keyword(s):  
Tympanic Membrane ◽  
Middle Ear ◽  
Inflammatory Reaction ◽  
Tympanic Membrane Perforation ◽  
Mucosal Inflammation ◽  
Controlled Study ◽  
Control Group ◽  
Otologic Surgery ◽  
Membrane Perforation ◽  
Brainstem Response

Problem Gelfoam has been used for decades in otologic surgery to support grafts and prostheses despite causing fibrosis and adhesions. More biocompatible packing materials could avoid these complications. This study compares Gelfoam with an injectable esterified hyaluronic acid, Merogel, as middle ear packing material after mucosal trauma. Methods A randomized, blinded, controlled study was performed in 17 juvenile guinea pigs. Middle ear surgery with mucosal trauma was performed and middle ears were packed with Merogel or Gelfoam; unpacked contralateral ears were used as controls. Auditory brainstem response (ABR) thresholds were measured in 4 frequencies pre-operatively, and repeated at 1, 2, and 6 weeks postoperatively. Gross analysis measured inflammatory reaction in each group. Statistical analysis was performed using ANOVA followed by post-hoc analysis for ABR thresholds and packing remaining at 6 weeks. Non-prametric tests were used for presence of mucosal inflammation, effusion and tympanic membrane perforation. Results ABR threshold changes from baseline were minor and comparable between the Merogel group and the control group. Threshold change was higher in the Gelfoam group. This difference was seen in each frequency tested at each time interval (all p<0.05). Gross analysis showed 1) Mucosal inflammation higher in the Gelfoam group (p<0.05), 2) Effusions were higher in the Gelfoam group but the difference was not significant (p=0.07), 3) Tympanic membrane perforation was equally rare between groups (p>0.05), 4) Unabsorbed packing was higher in the Gelfoam group (p<0.05); little Merogel was detectable at time of sacrifice. Conclusion Middle ear healing after surgery occurred similarly in the control group and the Merogel group. In contrast, the Gelfoam group demonstrated greater perturbation of hearing and a greater inflammatory reaction. Significance These results support Merogel as an alternative to Gelfoam in middle ear packing after otologic surgery. Support Research grant from Medtronic ENT.

scholarly journals A rare cause of acute tympanic membrane perforation: a case report

2018 ◽  
Vol 5 (1) ◽  
pp. 181
Author(s):  
C. J. Timna ◽  
Abhijit Kumar
Keyword(s):  
Case Report ◽  
Tympanic Membrane ◽  
Sudden Onset ◽  
Tympanic Membrane Perforation ◽  
Complete Healing ◽  
Accident And Emergency ◽  
Membrane Perforation ◽  
Accident And Emergency Department ◽  
Perforated Tympanic Membrane

<p class="abstract">This is a case report of 57yrs old gentleman who presented to accident and emergency department with complaints of sudden onset of left earache, tinnitus, feeling of fullness in the ear which he claimed to start following an episode of sudden sneezing, while he was trying to blow his nose by pinching it with 2 fingers. The tympanic membrane was ruptured. He was managed conservatively with analgesics. Spontaneous and complete healing of the perforated tympanic membrane occured within one month of follow up. It was shown that an episode of sneezing with nose closed can build up such a high pressure in the middle ear which can perforate a tympanic membrane.</p>

Potential ototoxicity from triamcinolone, neomycin, gramicidin and nystatin (Tri-Adcortyl™) cream

2005 ◽  
Vol 119 (1) ◽  
pp. 48-50 ◽  
Author(s):  
S P Thomas ◽  
J R Buckland ◽  
S R Rhys-Williams
Keyword(s):  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Sensorineural Hearing ◽  
Ear Canal ◽  
Membrane Perforation ◽  
Perforated Tympanic Membrane

Although rare, ototoxicity from the use of aminoglycoside drops is well recognized. Ototoxicity has not been described with the use of combination aminoglycoside-steroid-antifungal creams or ointments. We present the case of a 60-year-old man with a perforated tympanic membrane who suffered a total hearing loss after the instillation of cream containing triamcinolone, neomycin, gramicidin and nystatin (Tri-Adcortyl™ cream) into his ear canal. On balance, we believe that a number of potentially ototoxic constituents in this cream were responsible. Other possible causes of sensorineural hearing loss and the possible mechanisms of ototoxicity of this cream are discussed. The reasons why such creams may be particularly ototoxic, compared with drops, are also considered. The authors caution against the use of such creams or ointments in the ear if there is any suspicion of a tympanic membrane perforation.

An observational study of the management of traumatic tympanic membrane perforations

2002 ◽  
Vol 116 (3) ◽  
pp. 181-184 ◽  
Author(s):  
J. E. O. Amadasun
Keyword(s):  
Observational Study ◽  
Prospective Study ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Control Group ◽  
Tympanic Membrane Perforations ◽  
Membrane Perforation ◽  
A Prospective Study

Controversies of how best to treat fresh tympanic membrane perforations have always existed. While some otolaryngologists prefer the paper patch method, others prefer modified myringoplasty. A prospective study is needed to investigate the most effective and least expensive management of this common ear trauma.This study examined prospectively, in three sections, a group of patients with a cellophane patch (n = 6), another group with a gentamicin ointment seal (n = 15) and a control group (n = 9) with a gentamicin plug placed at the distal end of the external auditory cavity. Successful healing of the traumatic tympanic membrane perforations was achieved in 50 per cent of the cellophane seal group, 86.7 per cent of the gentamicin ointment seal group and 77.8 per cent of the control group. This study shows that the management of a fresh tympanic membrane perforation should be limited to cleaning the traumatized ear and preventing infection.

A moist edge environment aids the regeneration of traumatic tympanic membrane perforations

2017 ◽  
Vol 131 (7) ◽  
pp. 564-571 ◽  
Author(s):  
Z C Lou ◽  
Z H Lou
Keyword(s):  
Growth Factors ◽  
Literature Review ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Crust Formation ◽  
Advantages And Disadvantages ◽  
Tympanic Membrane Perforations ◽  
Membrane Perforation ◽  
History Of ◽  
Topical Applications

AbstractObjective:To review the history of moist therapy used to regenerate traumatic tympanic membrane perforations.Study design:Literature review.Methods:The literature on topical agents used to treat traumatic tympanic membrane perforations was reviewed, and the advantages and disadvantages of moist therapy were analysed.Results:A total of 76 studies were included in the analysis. Topical applications of certain agents (e.g. growth factors, Ofloxacin Otic Solution, and insulin solutions) to the moist edges of traumatic tympanic membrane perforations shortened closure times and improved closure rates.Conclusion:Dry tympanic membrane perforation edges may be associated with crust formation and centrifugal migration, delaying perforation closure. On the contrary, moist edges inhibit necrosis at the perforation margins, stimulate proliferation of granulation tissue and aid eardrum healing. Thus, moist perforation margins upon topical application of solutions of appropriate agents aid the regeneration of traumatic tympanic membrane perforations.

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