A Technology for Genetic Identification of Varieties and Wild Forms of Grapes Based on Multilocus Microsatellite Analysis

2021 ◽  
Vol 37 (3) ◽  
pp. 85-95
Author(s):  
I.A. Shilov ◽  
E.N. Kislin ◽  
O.P. Malyuchenko ◽  
P.N. Kharchenko
Keyword(s):  
Multiplex Pcr ◽  
Microsatellite Loci ◽  
New Technologies ◽  
Selection Process ◽  
Electrophoretic Analysis ◽  
Genetic Identification ◽  
Dna Fragments ◽  
Allelic Ladder ◽  
Planting Material ◽  
Length Standard

The genotyping technology has been developed based on 9 microsatellite loci (VVS2, VVMD5, VVMD7, VVMD27, VVMD28, VVMD25, VVMD32, VrZAG62, VrZAG79). It can be used for efficient, accurate and fast identification of grape varieties and forms (genus Vitis). The proposed approach includes a multiplex PCR of all loci followed by electrophoretic analysis of DNA fragments in one capillary of a genetic analyzer. The application of an additional length standard, an allelic ladder, consisting of all possible DNA fragments of the analyzed microsatellite loci, is one of the key features of the technology, which ensures the accuracy and reproducibility of the results. The advantage of the proposed technology is the ability to standardize and automate the procedure using 96-well plates, which opens up the possibility of conducting mass analyses. As a result of the study of varieties and forms of Vitis genus species, genetic passports were obtained, according to which a dendrogram was constructed, reflecting the genetic relationship of the studied samples. The developed technology makes it possible to distinguish varieties and wild forms of grapes; it can be used for their identification and determination of genetic distance between them, as well as for assessment of planting material and protection of breeders' rights. Vitis, grapes, microsatellites, SSR, genotyping, multiplex PCR, DNA fragment analysis The work was carried out within the framework of the state assignment on the topic "Development of New Technologies for Genetic Analysis of Forms of Agricultural Plants to Accelerate and Control the Selection Process" (project no. 0574-2019-0003).

scholarly journals Ground-based “Pot-in-Pot” system and its effectiveness in growing Thuja occidentalis “Smaragd” planting material in a nursery

2021 ◽  
Vol 875 (1) ◽  
pp. 012092
Author(s):  
A N Tseplyaev ◽  
A A Tseplyaeva
Keyword(s):  
New Technologies ◽  
Root Zone ◽  
Effect Of Temperature ◽  
Forest Steppe ◽  
High Survival ◽  
Large Area ◽  
Thuja Occidentalis ◽  
Planting Material ◽  
Favorable Temperature ◽  
Intensive Growth

Abstract For the sparsely wooded regions of Russia, and in particular, the Central forest-steppe, new technologies for growing seedlings and seedlings of tree species are extremely important. It provids rapid growth and high survival rate, which is compared to classic technologies. The purpose of our experiment was to establish the effect of temperature screens from pots of various sizes on the temperature in the root zone of the substrate, and, as a consequence, on the growth and productivity of Thuja occidentalis “Smaragd” in plastic pots for three seasons. The most favorable temperature conditions for plants are formed in large pots, which is facilitated by smooth temperature drops in the substrate and a large area of contact between the substrate and the root system. Agrotechnical methods of growing plants have shown the main advantages of “Pot-in-Pot” technology. This is accelerated plant growth due to more efficient use of water and fertilizers on a specially prepared substrate under conditions of minimal temperature fluctuations. Plants, especially large ones, will have a favorable presentation due to better leafing, a well-developed crown, and a large crown diameter. High safety during wintering also increases the efficiency of the technology providing a higher yield and subsequent more intensive growth.

Starting with a product in mind – biosolids management design based on beneficial use goals

2016 ◽  
Vol 11 (2) ◽  
pp. 423-432 ◽  
Author(s):  
Lisa Boudeman ◽  
Lawrence Hentz ◽  
Joshua Gliptis
Keyword(s):  
Product Quality ◽  
New Technologies ◽  
Selection Process ◽  
The United States ◽  
Public Works ◽  
Master Planning ◽  
Beneficial Use ◽  
Solids Processing ◽  
Management Alternative ◽  
Key Aspects

Biosolids generators across the United States are beginning to reevaluate their processing and management programs, to consider new technologies and products. As part of this, they are considering new regulatory requirements, more flexible outlets, and the concept of biosolids as a product. Their focus is no longer technology centered, but rather on product quality and the best use for it. As part of their Long-Term Biosolids Master Planning effort, Howard County Department of Public Works fulfilled their goal of selecting a solids processing technology that reduces volume and generates a Class A exceptional quality biosolids product that could be use locally in agricultural or non-agricultural markets. In this case, product quality characteristics were important in entering local markets. Key aspects of the selection process are discussed, including establishing County goals, identifying and surveying local beneficial use markets, selecting a solids management alternative, and ultimately selecting the dryer technology fulfilling the County's objective.

Automated Assignment of Physical Effects to Functions Using Ports Based on Bond Graphs

2011 ◽  
Author(s):  
Bergen Helms ◽  
Hansjo¨rg Schultheiß ◽  
Kristina Shea
Keyword(s):  
New Technologies ◽  
Selection Process ◽  
Innovation Process ◽  
Oriented Graph ◽  
Computational Design ◽  
Graph Grammar ◽  
Automated Assignment ◽  
Bond Graphs ◽  
Design Synthesis ◽  
Physical Effects

Innovation processes are highly susceptible to cyclic influences, such as evolving knowledge due to new technologies. In order to cope with these challenge, computational support is required. Paper-based design methods have vast amounts of knowledge at their disposal in the form of design catalogues. However, lacking a computational implementation, these knowledge sources provide no support for considering dynamic influences in the innovation process. The presented method is targeted at making the physical effects contained in design catalogues available for computational design synthesis approaches. For this purpose, this paper introduces the notion of abstraction ports that is used to represent the valid mapping between functional operators and physical effects. For the automated assignment of abstraction ports, a method has been developed that analyzes the equation structure of physical effects. This approach is derived from the modeling technique of bond graphs and is independent of any selection process proposed by design catalogues. Moreover, it allows for the formalization of evolving knowledge in new physical effects that are not yet contained in design catalogues. The assignment of abstraction ports has been successfully validated through the formalization of the physical effects of two design catalogues. Future work comprises the integration of quantitative characteristics of physical effects and the realization within the object-oriented graph grammar system booggie.

Route Selection for Project Success: Addressing “Feeling/Perception” Issues

1998 ◽  
Author(s):  
M. Mohitpour ◽  
G. Von Bassenheim ◽  
Ardean Braun
Keyword(s):  
New Technologies ◽  
Selection Process ◽  
Cost Effective ◽  
Optimization Methods ◽  
Public Perceptions ◽  
Careful Consideration ◽  
Route Selection ◽  
Acceptable Solution ◽  
Pipeline Project ◽  
Selection For

Selecting a route for a pipeline right-of-way (ROW) generally consists of engineering (technical and economic), socioeconomic and biophysical components. To effectively select a route, simultaneous consideration must be given to all the components from the initiation of a project to the integration of all aspects of each throughout the route selection process. To successfully select a route which creates a win-win situation for all the stakeholders of a pipeline project, political/governmental issues, community and land owner views, public perceptions and other similar controlling factors (such as Safety, Health, Environment and Risk (SHER)) must be carefully analyzed and integrated into the process. It is the consideration of all these issues that will lead to a ROW which will provide a technically acceptable solution, which is at the same time the least expensive, economically viable and acceptable to the community it traverses. This paper will provide an overview of route selection techniques (including new technologies) used and the process generally practiced by pipeline designers, highlighting controlling issues and optimization methods that need to be utilized in order to achieve a cost effective route selection. It provides details on significant “Feeling/Perception” issues that can either thwart or, by careful consideration of these issues, lead to a successful pipeline project. An example of such a route selection process will be provided on a project located in rough and mountainous terrain, that has significant regulatory/governmental, land, environmental, indigenous and geological issues.

scholarly journals The Future of Schools from a European Union Perspective: Selecting, Analyzing and Disseminating the Most Innovative Approaches towards the School of Tomorrow

2005 ◽  
Vol 3 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Henrik Hansson ◽  
Scott Hall
Keyword(s):  
European Union ◽  
Information And Communication Technologies ◽  
New Technologies ◽  
Selection Process ◽  
Communication Technologies ◽  
Local Context ◽  
Learning Approaches ◽  
European Science ◽  
The Future ◽  
Information And Communication

What can we learn from European Union (EU) school projects about future learning approaches? The EU has invested massively in information and communication technologies (ICT) projects promoting innovative use of new technologies in school settings. The aim of the project ‘School Foresight’, with partners in Bulgaria, Estonia, Greece, Romania, the Czech Republic and Sweden, was to select and reflect on the best projects done so far. These projects were popularized and presented at the European Science Week in the autumn of 2004 and reached more than 10,000 students in five countries. This article describes and discusses the School Foresight project and the selection process of innovative EU projects showing the way towards the school of tomorrow. A number of best cases will be presented and discussed in a European, American and global context. The school of the future is both a question of what is possible and what is desired. Different scenarios might be preferable depending on local context, history, language, etc., or, are we all moving into the ‘global classroom’?

Isolation, characterization and multiplex PCR development of Bean Goose (Anser fabalis) microsatellite loci

2015 ◽  
Vol 157 (2) ◽  
pp. 641-646 ◽  
Author(s):  
Oddmund Kleven ◽  
Rolf T. Kroglund ◽  
Jan E. Østnes
Keyword(s):  
Multiplex Pcr ◽  
Microsatellite Loci ◽  
Bean Goose ◽  
Anser Fabalis

scholarly journals Biological fundamentals of hardneck garlic productivity of different reproductions

2021 ◽  
Vol 1 (98) ◽  
pp. 126-141
Author(s):  
V. V. Yatsenko ◽  
Keyword(s):  
Raw Materials ◽  
Selection Process ◽  
Physiological State ◽  
Sharp Decrease ◽  
Research Field ◽  
Planting Material ◽  
National University ◽  
Efficiency Of Selection ◽  
General Studies ◽  
The Impact

Providing the population with food, agriculture with planting material, and raw materials for the processing and canning industries is an urgent problem of garlic production. Currently, not studied yet the passage and changes in the production processes of garlic plants under the influence of reproduction, which in some way constrains the industrial production of planting material and marketable products in general. Studies on the impact of reproductions on changes in physiological state, plant resistance to disease and productivity were conducted during 2017–2020 in the research field of the Department of Vegetable Growing, Uman National University of Horticulture. During the biometric measurements, a significant change in the variation of approbation traits (height of the plant and scape, number of leaves and its size, number and weight of bulbils, cloves in the bulb) of garlic plants under the influence of reproduction, ie variety testing must indicate what is this generation. There was a significant decrease in the concentration of chlorophyll and the antioxidant enzymes activity in the leaves in III–V reproductions, which significantly affected the productivity and tolerance of plants. Indicators of plant damage disease on a natural infectious background indicated a sharp decrease in the resistance of plants of the Sofiivskyi cultivar in IV–V reproductions, Prometei and Lyubasha cultivarss in III–V. It was found that in the cultivar Sofiivskyi the largest mass of the bulb was formed in the first reproduction, while in the cultivars Prometheus and Lyubasha – in the second, and its gradual decrease to 21.2 % in the fifth reproduction. Yield had a similar trend, where the maximum reduction in yield was observed at 18.2; 28.0 and 17.0 % in the cultivars Sofiivskyi, Prometei and Lyubasha. The developed scientifically substantiated provisions will allow to increase efficiency of selection process and production of seed material.

scholarly journals New technologies in the recruitment and selection process

2018 ◽  
Vol 13 (3) ◽  
pp. 219-232
Author(s):  
Celina SOŁEK-BOROWSKA ◽  
Maja Wilczewska
Keyword(s):  
Big Data ◽  
New Technologies ◽  
Selection Process ◽  
Recruitment And Selection

Dobrze przeprowadzony proces rekrutacji jest niezwykle ważny dla organizacji, pozwala na dogłębną i obiektywną weryfikację kandydatów pod kątem spełnienia oczekiwań pracodawcy i ostatecznie prowadzi do ich zatrudnienia. Głównym celem artykułu jest analiza, w jaki sposób nowe technologie wpłynęły na proces rekrutacji. Proces rekrutacji zostanie przedstawiony na dwóch przykładach: mianowicie ItutorGroup i sieci luksusowych salonów odzieżowych. Rekrutacja w grupie ITutor opierała się wyłącznie na nowych technologiach z ograniczoną ingerencją czynnika ludzkiego. Strategia rekrutacji opierała się na rekrutacji wideo. Drugie studium przypadku opisuje współpracę między agencją doradztwa personalnego a luksusową siecią sprzedaży detalicznej, mającą na celu rekrutację i zatrudnianie sprzedawców w Polsce poprzez wprowadzenie działań Big Data w mediach społecznościowych oraz proces scoutingu. Wyniki wskazują, że e-rekrutacja przekształca tradycyjny proces rekrutacji w proces niezależny od czasu, oparty na współpracy. Najważniejsze zmiany są rejestrowane, istnieje zwiększona podzielność głównych zadań rekrutacyjnych. Głównym zadaniem rekrutera staje się komunikacja z kandydatami. Z drugiej strony w niektórych projektach rekrutacyjnych nie można polegać wyłącznie na nowych technologiach. Rekrutacja w postaci bezpośrednich kontaktów z potencjalnymi kandydatami wydłużyła proces w czasie, ale pozwoliła na uzyskanie „skrojonych na miarę” kandydatów przygotowanych i sprawdzonych w miejscu pracy. Strategia rekrutacji i selekcji oparta na nowoczesnych technologiach wymaga doświadczonego i kompetentnego zespołu, dwie niewątpliwe korzyści to: ograniczenie długości procesu w czasie i możliwość obniżenia kosztów.

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