Preparation of Catalase Cross-Linked Aggregates Based on Vaterite Matrix

2021 ◽  
Vol 37 (4) ◽  
pp. 51-59
Author(s):  
N.L. Eremeev ◽  
N.G. Balabushevich ◽  
D.V. Volod'kin ◽  
N.L. Klyachko
Keyword(s):  
Specific Activity ◽  
High Specific Activity ◽  
Development Program ◽  
Spherical Shape ◽  
Native Enzyme ◽  
Narrow Particle Size Distribution ◽  
State University ◽  
Sem Images ◽  
The Cross ◽  
Moscow State University

A new technique has been developed for the synthesis of cross-linked catalase aggregates by treatment of the enzyme incorporated into the pores of vaterite microspheres with glutaraldehyde and subsequent dissolving of the inorganic matrix. The resulting aggregates have a spherical shape, a narrow particle size distribution and a high specific activity of the enzyme. The number and enzymatic activity of the cross-linked aggregates strongly depends on the rate of the matrix dissolution: mild dissolution conditions made it possible to increase the number of formed protein particles, the residual specific catalase activity of which is only 2 times less than that of the native enzyme. The storage stability of the cross-linked aggregates is comparable to that of the native enzyme of the same concentration. Key words: vaterite, glutaraldehyde, immobilized preparations, intermolecular cross-linking, co-precipitation, CLEAs. Acknowledgment - The authors are grateful to the staff of the M.V. Lomonosov Moscow State University A.N. Prusov for help in obtaining TEM images and A.A. Tatarintseva for assistance in obtaining SEM images. Funding - This work was supported in part by Lomonosov Moscow State University (Registration Theme АААА-А21-121011290089-4). The authors also acknowledge Lomonosov Moscow State University Development Program PNR 5.13.

scholarly journals Cross'Cultural Communication Training: Dedicated to the Memory of Tatiana G. Stefanenko

2020 ◽  
Vol 17 (1) ◽  
pp. 174-182
Author(s):  
Olga V. Luneva
Keyword(s):  
Social Psychology ◽  
Significant Contribution ◽  
Communication Training ◽  
Cross Cultural ◽  
Cultural Communication ◽  
State University ◽  
Training Project ◽  
Cross Cultural Communication ◽  
The Cross ◽  
Moscow State University

The article is dedicated to the memory of Tatiana G. Stefanenko, Professor of the Department of Social Psychology, Lomonosov Moscow State University, who made a significant contribution to ethnopsychology as well as to the training of ethnopsycologists. The focus is on her participation in developing and testing the cross-cultural communication training project and some facets of the researcher’s personality.

Effects of Heparin Oligosaccharides with High Affinity for Antithrombin III in Experimental Venous Thrombosis

1982 ◽  
Vol 47 (03) ◽  
pp. 244-248 ◽  
Author(s):  
D P Thomas ◽  
Rosemary E Merton ◽  
T W Barrowcliffe ◽  
L Thunberg ◽  
U Lindahl
Keyword(s):  
Antithrombin Iii ◽  
Specific Activity ◽  
High Specific Activity ◽  
Factor Xa ◽  
Blood Levels ◽  
Thrombin Time ◽  
High Affinity ◽  
Very High

SummaryThe in vitro and in vivo characteristics of two oligosaccharide heparin fragments have been compared to those of unfractionated mucosal heparin. A decasaccharide fragment had essentially no activity by APTT or calcium thrombin time assays in vitro, but possessed very high specific activity by anti-Factor Xa assays. When injected into rabbits at doses of up to 80 ¼g/kg, this fragment was relatively ineffective in impairing stasis thrombosis despite producing high blood levels by anti-Xa assays. A 16-18 monosaccharide fragment had even higher specific activity (almost 2000 iu/mg) by chromogenic substrate anti-Xa assay, with minimal activity by APTT. When injected in vivo, this fragment gave low blood levels by APTT, very high anti-Xa levels, and was more effective in preventing thrombosis than the decasaccharide fragment. However, in comparison with unfractionated heparin, the 16-18 monosaccharide fragment was only partially effective in preventing thrombosis, despite producing much higher blood levels by anti-Xa assays.It is concluded that the high-affinity binding of a heparin fragment to antithrombin III does not by itself impair venous thrombogenesis, and that the anti-Factor Xa activity of heparin is only a partial expression of its therapeutic potential.

Autoprothrombin C Activity from Prothrombin with Snake Venom or Trypsin

1962 ◽  
Vol 08 (03) ◽  
pp. 425-433 ◽  
Author(s):  
Ewa Marciniak ◽  
Edmond R Cole ◽  
Walter H Seegers
Keyword(s):  
Snake Venom ◽  
Thrombolytic Agent ◽  
Sodium Citrate ◽  
Specific Activity ◽  
High Specific Activity ◽  
Citrate Solution ◽  
Clotting Factors ◽  
Activation Products ◽  
Russell’S Viper ◽  
Autoprothrombin C

SummarySuitable conditions were found for the generation of autoprothrombin C from purified prothrombin with the use of Russell’s viper venom or trypsin. DEAE chromatographed prothrombin is structurally altered and has never been found to yield autoprothrombin C and also did not yield it when Russell’s viper venom or trypsin were used. Autoprothrombin C is derived from prothrombin with tissue extract thromboplastin, but not in large amounts with the intrinsic clotting factors. With the latter thrombin and autoprothrombin III are the chief activation products. Autoprothrombin III concentrates were prepared from serum and upon activation with 25% sodium citrate solution or with Russell’s viper venom large amounts of autoprothrombin C were obtained, and this was of high specific activity. Theoretically trypsin is not a thrombolytic agent, but on the contrary should lead to intravascular clotting.

Peronosporales of Skadovsky Zvenigorod Biological Station (Moscow Region)

2018 ◽  
Vol 52 (1) ◽  
pp. 91-100
Author(s):  
E. Yu. Blagoveshchenskaya
Keyword(s):  
Downy Mildew ◽  
European Part ◽  
Moscow Region ◽  
State University ◽  
Biological Station ◽  
Annotated List ◽  
European Part Of Russia ◽  
First Time ◽  
Moscow State University

The paper provides the results of seven-year study of downy mildew on Skadovsky Zvenigorod Biological Station of Moscow State University (ZBS MSU, Moscow Region). A total of 29 species of Peronosporales (Oomycota) were revealed during the study. An annotated list of species is presented, among them Peronospora anemones is recorded for the first time for Russia, P. chelidonii and P. stachydis are new for the European part of Russia, 8 species are new for the Moscow Region.

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