scholarly journals Formulating a functional drink with antiosteoporosis effects

2021 ◽  
Vol 9 (2) ◽  
pp. 354-363
Author(s):  
Marietta Aslanova ◽  
Anastasia Semenova ◽  
Olga Derevitskaya
Keyword(s):  
Connective Tissue ◽  
Dietary Fiber ◽  
Bone Tissue ◽  
Blood Parameters ◽  
Female Rats ◽  
Laboratory Rats ◽  
Functional Product ◽  
Pumpkin Powder ◽  
Functional Drink ◽  
Peptide Fractions

Introduction. Osteoporosis is one of the most common diseases of the musculoskeletal system in modern clinical practice. Its prevention and treatment requires a diet with a sufficient intake of calcium, vitamins, and connective tissue proteins that regenerate cartilage and bone tissue. We aimed to formulate a functional product based on collagen fermentolysate to prevent osteoporosis and prove its effects in experiments on laboratory rats. Study objects and methods. Our study objects were collagen fermentolysate obtained from pork ears and legs (1:1) and the functional product based on it. The biological experiment was carried out on Wistar female rats exposed to osteoporosis through complete ovariectomy. Their femurs were analyzed for the contents of phosphorus, magnesium, and calcium, as well as cytometric and biochemical blood parameters. Results and discussion. The formulated functional product based on collagen fermentolysate contained 41% of the most easily assimilable peptide fractions with a low molecular weight of 10 to 20 kDa. Other components included pumpkin powder, dietary fiber, calcium, chondroprotectors, and vitamins E, C, and D3. Compared to the control, the experimental rats that received the functional product had increased contents of calcium and magnesium in the bone tissue (by 25.0 and 3.0%, respectively), a decreased content of phosphorus (by 7.0%), a calcium-to-phosphorus ratio restored to 2.4:1.0, and a higher concentration of osteocalcin in the blood serum (by 15%). Conclusion. The developed functional product based on collagen fermentolysate can be used as an additional source of connective tissue protein, calcium, vitamins C, E, and D3, dietary fiber, and chondroprotectors to prevent osteoporosis.

Neospora caninum (Protozoa: Apicomplexa) infections in rats

1990 ◽  
Vol 68 (7) ◽  
pp. 1595-1599 ◽  
Author(s):  
David S. Lindsay ◽  
J. P. Dubey
Keyword(s):  
Rattus Norvegicus ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Hepatic Necrosis ◽  
Female Rats ◽  
Methylprednisolone Acetate ◽  
Laboratory Rats ◽  
Experimental Inoculation ◽  
Caninum Tachyzoites ◽  
Subcutaneous Inoculation

The susceptibility of laboratory rats (Rattus norvegicus) to experimental inoculation with tachyzoites of Neospora caninum was examined. Groups of female rats were intramuscularly injected with 0, 2, or 4 mg of methylprednisolone acetate (MPA) 7 days prior to, and on the day of, subcutaneous inoculation with 0 or 1.5 × 105 tachyzoites. Clinical signs of disease or deaths did not occur in rats given nothing or only N. caninum tachyzoites. Rats given only 4 mg MPA failed to grow as well as rats given nothing or only N. caninum tachyzoites but were otherwise healthy. All of 20 rats given 4 mg MPA and tachyzoites died of hepatitis and pneumonia within 12 days postinoculation. Hepatic necrosis was the most striking lesion seen in these rats, and other milder lesions consisted of pneumonia, encephalitis, and myositis. The response of rats given 2 mg MPA and tachyzoites was less severe. Three of 20 rats died with encephalitis, myositis, hepatitis, and pancreatitis. Mild lesions, but no N. caninum tachyzoites, were seen in 3 of 14 rats inoculated only with tachyzoites. Rats given the 4 mg MPA treatment and inoculated with N. caninum tachyzoites appear to be suitable subjects for examining acute neosporosis and could be used in studies designed to examine treatment of acute disease.

Biological Response to Wollastonite Doped α-Tricalcium Phosphate Implants in Hard and Soft Tissues in Rats

2008 ◽  
Vol 396-398 ◽  
pp. 7-10 ◽  
Author(s):  
Ana Maria Minarelli Gaspar ◽  
Sybele Saska ◽  
R. García Carrodeguas ◽  
A.H. De Aza ◽  
P. Pena ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Rattus Norvegicus ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
New Bone Formation ◽  
Rat Bone

The biological response following subcutaneous and bone implantation of β-wollastonite(β-W)-doped α-tricalcium phosphate bioceramics in rats was evaluated. Tested materials were: tricalcium phosphate (TCP), consisting of a mixture of α- and β-polymorphs; TCP doped with 5 wt. % of β-W (TCP5W), composed of α-TCP as only crystalline phase; and TCP doped with 15 wt. % of β-W (TCP15), containing crystalline α-TCP and β-W. Cylinders of 2x1 mm were implanted in tibiae and backs of adult male Rattus norvegicus, Holtzman rats. After 7, 30 and 120 days, animals were sacrificed and the tissue blocks containing the implants were excised, fixed and processed for histological examination. TCP, TCP5W and TCP15W implants were biocompatible but neither bioactive nor biodegradable in rat subcutaneous tissue. They were not osteoinductive in connective tissue either. However, in rat bone tissue β-W-doped α-TCP implants (TCP5W and TCP15W) were bioactive, biodegradable and osteoconductive. The rates of biodegradation and new bone formation observed for TCP5W and TCP15W implants in rat bone tissue were greater than for non-doped TCP.

The case of fibrous osteodystrophy (Recklinghausen disease)

1937 ◽  
Vol 33 (1) ◽  
pp. 91-95
Author(s):  
N. A. Serebrennikov
Keyword(s):  
Bone Marrow ◽  
Connective Tissue ◽  
Giant Cell ◽  
Bone Tissue ◽  
Fibrous Connective Tissue ◽  
Cell Tissue ◽  
Recklinghausen Disease

In 1891, Recklinghausen first accurately described fibrous osteodystrophy, putting forward five main provisions that determine the essence of this disease: 1) replacement of cellular and adipose bone marrow with fibrous connective tissue; 2) resorption of bone tissue by lacunar suction; 3) metaplastic and partially osteoblastic neoplasm of osteoid and bone tissue; 4) tumor-like growths of fibrous and giant cell tissue, such as epulids, resembling giant cell sarcomas with a brown tint due to the presence of hemosiderin in the growths - "Braune tumoren" - by German authors.

Are wild rodents attracted to lure laboratory rats?

2014 ◽  
Vol 20 (1) ◽  
pp. 108
Author(s):  
A C Gsell ◽  
M N H Seabrook-Davison ◽  
D H Brunton
Keyword(s):  
Early Detection ◽  
Female Rats ◽  
Wild Rodents ◽  
Laboratory Rats ◽  
Male And Female Rats ◽  
In The Wild ◽  
Wild Rats ◽  
Developing Rat ◽  
Free Status

invasions to New Zealand’s unique biodiversity, ‘pest-free’ offshore and mainland island refuges have been created. The success of this approach depends on the long term maintenance of the pest-free status of these refuges. Because the occurrence of rodent incursions is an on-going risk, early detection and elimination of invading animals is crucial. We conducted field-based experiments to determine if lures of live female and male laboratory Norway rats (Rattus norvegicus) held in cages in the wild, and lures consisting of urine-soaked rodent bedding could be used to detect the presence of wild rodents. We found that the use of live rodents and bedding significantly increased the probability of detecting wild rodents, although we were not able to determine the sex, age or number of wild rodents attracted to each live rodent station. We also found that wild rats were equally attracted to the scent of male and female rats i.e., lures and bedding. Our approach provides potential as a tool for early detection of rodents in vulnerable refuges and we suggest that further research is needed to investigate the feasibility of developing rat scented monitoring stations.

The incidence of chronic progressive nephrosis in young Sprague-Dawley rats from two different breeders

1998 ◽  
Vol 32 (4) ◽  
pp. 477-482 ◽  
Author(s):  
Maud Palm
Keyword(s):  
Kidney Function ◽  
Young Female ◽  
Female Rats ◽  
Common Finding ◽  
Sprague Dawley ◽  
Laboratory Rats ◽  
Toxicity Studies ◽  
Sprague Dawley Rats ◽  
Males And Females

Chronic progressive nephrosis (CPN) in rats may not only become a problem in long-term toxicity studies but also in short-term studies, if the breeding stock is not carefully selected with respect to the kidney function. This paper presents differences in kidney function between young rats of the same strain, Sprague-Dawley, but from two different breeders ('set A' and 'set B' rats). In set A rats, protein in the urine was present in the males, which is a common finding. In set B rats, not only the males but also the females excreted protein in the urine. The method used to detect protein in the urine does not normally show a positive protein result in the young female rats. At the age of 3 months signs of chronic progressive nephrosis were observed in 55% of the males and in 15% of the females in set B. Two months later, the incidence had increased to about 70–80% in males and 50% in females. At 8 months, the incidence was similar, but the severity had increased. These values were compared with those obtained from the set A rats, none of which showed any signs of the disease at the age of 5 months and only 5% of the males and females at the age of 8 months. The results indicated that an increased excretion of protein in the urine may be used as an indicator for chronic progressive nephrosis in the rat and that not only the strain but also the source is important in selecting laboratory rats for toxicity studies.

CELLULAR AND SUBCELLULAR LOCALIZATION OF 3H-OESTRADIOL IN THE OVIDUCT AND UTERUS OF THE NEONATAL RAT

1974 ◽  
Vol 76 (3) ◽  
pp. 570-575 ◽  
Author(s):  
Peter J. Sheridan ◽  
Madhabananda Sar ◽  
Walter E. Stumpf
Keyword(s):  
Body Weight ◽  
Connective Tissue ◽  
Subcellular Localization ◽  
Neonatal Rat ◽  
Female Rats ◽  
Methyl Green ◽  
Luminal Epithelium ◽  
Frozen Sections

ABSTRACT Two day old female rats were injected sc with 1.0 μg of 3H-oestradiol per 100 g body weight and killed 2 h later. Oviducts and uteri were removed, mounted on tissue holders, and frozen in liquefied propane. Four μm frozen sections were cut in a cryostat and mounted according to the dry-mount and thaw-mount autoradiographic procedures. The sections were exposed at –15°C for different periods of time, then photographically processed and stained with methyl green-pyronin. Radioactivity was found concentrated in nuclei of cells of the luminal epithelium in the uterus, but not in the oviduct, while cells of the connective tissue and muscularis showed nuclear concentration of radioactivity in both organs. Pretreatment with oestradiol inhibited the nuclear concentration of radioactivity, whereas pretreatment with 5α-dihydrotestosterone had no effect. It is concluded that both the uterus and oviduct of the neonatal rat have receptors for oestrogen as early as 2 days after birth.

scholarly journals Reparative histogenesis of bone tissue in femoral fractures in rats using biocomposite material along with immunocorrection

2021 ◽  
pp. 248-253
Author(s):  
V. V. Reshetnyak ◽  
V. V. Burdeyniy ◽  
V. V. Pronin ◽  
Ye. A. Iskaliev
Keyword(s):  
Connective Tissue ◽  
Bone Tissue ◽  
Femoral Fractures ◽  
Live Weight ◽  
High Ratio ◽  
Cartilage Tissue ◽  
Control Group ◽  
Bone Fragments ◽  
Bone Callus ◽  
Biocomposite Material

The paper studies the effect of the RVI biocomposite material belonging to the group of osteoplastic biocomposite materials, the RV-2 immunomodulator – a synthetic dipeptide inducing an immunocorrective effect, and combinations of these drugs on the reparative histogenesis of bone tissue in femoral fractures in rats. It was found that the remodeling of the primary bone callus into the secondary one in the fracture of the studied animals was of a diverse nature. This process was the most pronounced in the group where the components were used in complex, i.e. the bone defect was filled with RVI during the surgery, as well as RV-2 was injected intramuscularly to rats at a dose of 10 mcg per 1 kg of live weight for five days, starting immediately after the surgery. Well-formed coarse-fibrous connective tissue callus was recorded in animals of this group. The connective tissue was stained more intensely which indicates a denser arrangement of fibers in the callus. Focal cartilage tissue spanning bone fragments was observed within the callus. At the periphery of the site the cartilaginous callus was subjected to endochondral ossification with replacement by coarse-fibrous trabeculae with elements of lamellar bone tissue having haversian canals in the center. The inter-girdle spaces were filled with elements of the myeloid bone marrow in the forming bone tissue. Markedly proliferated osteoblasts were visible in the cambial layer of the periosteum. The bone tissue ratio increased up to (60.21 ± 2.62)%, which significantly exceeded the same indicator in the control group and in all experimental groups. The low content of connective tissue and the high ratio of bone tissue indicated more active osteogenesis processes and reparative regeneration in comparison with other groups.

scholarly journals FEATURES OF SKIN WOUNDS HEALING UNDER CHRONIC HYPERGLYCEMIA AND IMPROVEMENT OF THEIR TREATMENT METHODS

2021 ◽  
Vol 74 (5) ◽  
pp. 1174-1179
Author(s):  
Olena S. Maksymova ◽  
Svitlana M. German ◽  
Pavlo O. Moskalenko ◽  
Viktoriia O. Yasenok ◽  
Olena M. Gortynska ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Vascular Remodeling ◽  
Platelet Rich Plasma ◽  
Skin Wounds ◽  
Pathological Changes ◽  
Skin Injuries ◽  
Laboratory Rats ◽  
Chronic Hyperglycemia ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

The aim: The work was aimed to study the histological, morphometric and planimetric features of skin regeneration in mature rats with chronic hyperglycemia under the influence of platelet-rich plasma. Materials and methods: 60 mature white laboratory rats were used. The animals were divided into three groups (I – control with mechanical skin injuries; II – rats with chronic hyperglycemia and modeled mechanical skin injuries; III – animals with the chronic hyperglycemia and modeled mechanical skin injuries which were injected with the platelet-rich plasma). The samples were studied using light microscopy.Statistical data processing was performed using SPSS-17. Results: On the 21st day, the epithelialization of control mature rats wound was almost complete. The epithelium contained all layers without pathological changes. The new dermis has been reorganized into papillary and reticular layers. On the 21st day, the wound of rats with chronic hyperglycemia was not completely covered with the epidermis. The connective tissue of the dermis was disorganized. On the 21st day, the wound epithelialization was also more complete in mature rats with chronic hyperglycemia received platelet-rich plasma compared to the rats with chronic hyperglycemia. The dermis contained a large number of blood vessels with normal, full-blooded lumens. Conclusions: The chronic hyperglycemia leads to disruption of epithelialization processes, angiogenesis, a delay in the reorganization of dermis connective tissue, and vascular remodeling. The injections of autologous platelet-rich plasma promote faster angiogenesis, reduce inflammation, and accelerate wound epithelialization.

Sign in / Sign up

Export Citation Format

Share Document