Studying the effect of Egyptian propolis on antimicrobial properties of glass ionomer cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.

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Extract of Propolis on Resin-Modified Glass Ionomer Cement: Effect on Mechanical and Antimicrobial Properties and Dentin Bonding Strength

International Journal of Biomaterials ◽

10.1155/2021/5597837 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Narges Panahandeh ◽

Fatemeh Adinehlou ◽

Seyedeh Mahsa Sheikh-Al-Eslamian ◽

Hassan Torabzadeh

Keyword(s):

Antibacterial Activity ◽

Flexural Strength ◽

Bond Strength ◽

Shear Bond Strength ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Control Group ◽

Glass Ionomer ◽

Resin Modified Glass Ionomer ◽

Ionomer Cement

This study assessed the effect of addition of aqueous extract of propolis in different concentrations on the mechanical and antimicrobial properties of resin-modified glass ionomer cement (RMGIC). In this in vitro study, powder of Fuji II LC RMGIC was mixed with 25% and 50% aqueous extracts of propolis. Samples (n = 15 for shear bond strength, n = 5 for flexural strength, and n = 20 for the antibacterial activity test) were fabricated using this mixture. The buccal and lingual surfaces of 23 premolars were ground to expose dentin. Tygon tubes were filled with cement, bonded to dentin, and subjected to bond or the flexural strength test in a universal testing machine. Antibacterial activity was assessed using the disc diffusion and well-plate techniques against S. mutans. Data were analyzed using one-way ANOVA and Tukey’s test. The three groups showed significant differences ( p < 0.001). The 50% propolis group had the lowest flexural and shear bond strength. The control group had the highest flexural and shear bond strength. No growth inhibition zone was noted around any of the discs. It can be concluded that addition of propolis to RMGIC did not confer any antibacterial activity against S. mutans and decreased the flexural and shear bond strength of RMGIC.

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Green synthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against dental biofilm and its effect on compressive strength

10.21203/rs.3.rs-35758/v1 ◽

2020 ◽

Author(s):

sanaa M. F. gad El-Rab ◽

Enas Tawfik Enan ◽

Amal A. Ashour ◽

Sakeenabi Basha ◽

Nayef H. Felemban

Keyword(s):

Antimicrobial Activity ◽

Compressive Strength ◽

Antimicrobial Agents ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Test System ◽

Glass Ionomer ◽

Dental Biofilm ◽

Group D ◽

Ionomer Cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of their failure. The urge for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents. Objectives Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength of GIC was also evaluated. Material and methods Biosynthesis of AgNPs was done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity. The distribution of biofilm was examined via scanning electron microscope. Compressive strength (CS) of the tested material was measured using a Material Test System (MTS). Results UV-Visible spectrum showed a peak of 429 nm.TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5–25.8 nm. The maximum inhibitory zone was recorded for group D against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused insignificant effect on compressive strength of GIC. Conclusion Cupressus macrocarpa extract biosynthesized AgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against dental biofilm. The agents can be safely added without impairing mechanical properties of the set cement.

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Influence of salvadora persica (miswak) extract on physical and antimicrobial properties of glass ionomer cement

European Archives of Paediatric Dentistry ◽

10.1007/bf03262774 ◽

2011 ◽

Vol 12 (1) ◽

pp. 22-25 ◽

Cited By ~ 11

Author(s):

A. El-Tatari ◽

J. J. de Soet ◽

A. J. de Gee ◽

M. Abou Shelib ◽

W. E. van Amerongen

Keyword(s):

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Glass Ionomer ◽

Salvadora Persica ◽

Ionomer Cement

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A new self-curing resin-modified glass-ionomer cement for the direct bonding of orthodontic brackets in vivo☆, ☆☆

American Journal of Orthodontics and Dentofacial Orthopedics ◽

10.1016/s0889-5406(98)70112-x ◽

1998 ◽

Vol 113 (4) ◽

pp. 384-386 ◽

Cited By ~ 1

Author(s):

J FRICKER

Keyword(s):

Glass Ionomer Cement ◽

Orthodontic Brackets ◽

Glass Ionomer ◽

Resin Modified Glass Ionomer ◽

Ionomer Cement

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Microleakage of class II restoration with zirconia inlay: An in vitro study

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2019.5.9 ◽

2019 ◽

pp. 61-67

Author(s):

Xuan Anh Ngoc Ho ◽

Anh Chi Phan ◽

Toai Nguyen

Keyword(s):

Glass Ionomer Cement ◽

Class Ii ◽

Resin Cement ◽

Glass Ionomer ◽

Resin Modified Glass Ionomer ◽

Group 2 ◽

Self Etch ◽

Ionomer Cement ◽

Group 1 ◽

Composite Restoration

Background: Class II restoration with zirconia inlay is concerned by numerous studies about the luting coupling between zirconia inlay and teeth. The present study was performed to evaluate the microleakage of Class II zirconia inlayusing two different luting agents and compare to direct restoration using bulk fill composite. Aims: To evaluate the microleakage of Class II restorations using three different techniques. Materials and methods: The study was performed in laboratory with three groups. Each of thirty extracted human teeth was prepared a class II cavity with the same dimensions, then these teeth were randomly divided into 3 groups restored by 3 different approaches. Group 1: zirconia inlay cemented with self-etch resin cement (Multilink N); Group 2: zirconia inlay cemented with resin-modified glass ionomer cement (Fuji Plus); Group 3: direct composite restoration using bulk fill composite(Tetric N-Ceram Bulk Fill). All restorations were subjected to thermal cycling (100 cycles 50C – 55 0C), then immersed to 2% methylene blue solution for 24 hours. The microleakage determined by the extent of dye penetration along the gingival wall was assessed using two methods: quantitative and semi-quantitative method. Results: Among three types of restorations, group 1 demonstrated the significantly lower rate of leakage compared to the others, while group 2 and 3 showed no significant difference. Conclusion: Zirconia inlay restoration cemented with self-etch resin cement has least microleakage degree when compare to class II zirconia inlay restoration cemented with resin-modified glass ionomer cement and direct composite restoration using bulk fill composite. Key words: inlay, zirconia ceramic, class II restoration, microleakage.

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