scholarly journals Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

2020 ◽  
Author(s):  
Enas Tawfik Enan ◽  
Amal A. Ashour ◽  
Sakeenabi Basha ◽  
Nayef H. Felemban ◽  
sanaa M. F. gad El-Rab

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.

2020 ◽  
Author(s):  
sanaa M. F. gad El-Rab ◽  
Enas Tawfik Enan ◽  
Amal A. Ashour ◽  
Sakeenabi Basha ◽  
Nayef H. Felemban

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of their failure. The urge for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents. Objectives Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength of GIC was also evaluated. Material and methods Biosynthesis of AgNPs was done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity. The distribution of biofilm was examined via scanning electron microscope. Compressive strength (CS) of the tested material was measured using a Material Test System (MTS). Results UV-Visible spectrum showed a peak of 429 nm.TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5–25.8 nm. The maximum inhibitory zone was recorded for group D against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused insignificant effect on compressive strength of GIC. Conclusion Cupressus macrocarpa extract biosynthesized AgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against dental biofilm. The agents can be safely added without impairing mechanical properties of the set cement.


Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 528
Author(s):  
Amal Adnan Ashour ◽  
Sakeenabi Basha ◽  
Nayef H. Felemban ◽  
Enas T. Enan ◽  
Amal Ahmed Alyamani ◽  
...  

In the present study, Zingiber officinale is used for the synthesis of Zingiber officinale capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs, lyophilized miswak, and chlorhexidine diacetate (CHX) against oral microbes. Five groups of the disc-shaped GIC specimens were prepared. Group A: lyophilized miswak and GIC combination, Group B: ZOE-AgNPs and GIC combinations, Group C: CHX and GIC combination, Group D: ZOE-AgNPs + CHX + GIC; Group E: Conventional GIC. Results confirmed the successful formation of ZOE-AgNPs that was monitored by UV-Vis sharp absorption spectra at 415 nm. The X-ray diffractometer (XRD) and transmission electron microscope (TEM) results revealed the formation of ZOE-AgNPs with a mean size 10.5–14.12 nm. The peaks of the Fourier transform infrared spectroscopy (FTIR) were appearing the involvement of ZOE components onto the surface of ZOE-AgNPs which played as bioreducing, and stabilizing agents. At a 24-h, one-week and three-week intervals, Group D showed the significantly highest mean inhibitory zones compared to Group A, Group B, and Group C. At microbe-level comparison, Streptococcus mutans and Staphylococcus aureus were inhibited significantly by all the specimens tested except group E when compared to Candida albicans. Group D specimens showed slightly higher (45.8 ± 5.4) mean compressive strength in comparison with other groups. The combination of GIC with ZOE-AgNPs and chlorhexidine together enhanced its antimicrobial efficacy and compressive strength compared to GIC with ZOE-AgNPs or lyophilized miswak or chlorhexidine combination alone. The present study revealed that The combination of GIC with active components of ZOE-AgNPs and chlorhexidine paves the way to lead its effective nano-dental materials applications.


2013 ◽  
Vol 3 ◽  
pp. 245-248 ◽  
Author(s):  
P Sri Chandana ◽  
Swapna Munaga ◽  
M Narender Reddy ◽  
Dishasaraswathi Devabhaktuni ◽  
Challakolusu Lakshmi Swathi

2015 ◽  
Vol 9 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Ankur Gupta ◽  
Deepak Singh ◽  
Praveen Raj ◽  
Himanshu Gupta ◽  
Sheetal Verma ◽  
...  

2007 ◽  
Vol 21 (3) ◽  
pp. 204-208 ◽  
Author(s):  
André Mallmann ◽  
Jane Clei Oliveira Ataíde ◽  
Rosa Amoedo ◽  
Paulo Vicente Rocha ◽  
Letícia Borges Jacques

The purpose of this study was to evaluate the compressive strength of two glass ionomer cements, a conventional one (Vitro Fil® - DFL) and a resin-modified material (Vitro Fil LC® - DFL), using two test specimen dimensions: One with 6 mm in height and 4 mm in diameter and the other with 12 mm in height and 6 mm in diameter, according to the ISO 7489:1986 specification and the ANSI/ADA Specification No. 66 for Dental Glass Ionomer Cement, respectively. Ten specimens were fabricated with each material and for each size, in a total of 40 specimens. They were stored in distilled water for 24 hours and then subjected to a compressive strength test in a universal testing machine (EMIC), at a crosshead speed of 0.5 mm/min. The data were statistically analyzed using the Kruskal-Wallis test (5%). Mean compressive strength values (MPa) were: 54.00 ± 6.6 and 105.10 ± 17.3 for the 12 mm x 6 mm sample using Vitro Fil and Vitro Fil LC, respectively, and 46.00 ± 3.8 and 91.10 ± 8.2 for the 6 mm x 4 mm sample using Vitro Fil and Vitro Fil LC, respectively. The resin-modified glass ionomer cement obtained the best results, irrespective of specimen dimensions. For both glass ionomer materials, the 12 mm x 6 mm matrix led to higher compressive strength results than the 6 mm x 4 mm matrix. A higher variability in results was observed when the glass ionomer cements were used in the larger matrices.


2018 ◽  
Vol 20 (3) ◽  
pp. 61-69
Author(s):  
L.O.L. Bohner DDS, MSc, Ph ◽  
L.H.M. Prates

The objective of this study was to evaluate the compressive strength of a glass ionomer cement (GIC) under the influence of varnish protection and dietary fluids. Eighty cylindrical test specimens were made from GIC and distributed into four groups (G1, G2, G3, G4) according to the dietary fluid. Each group was further divided into subgroups A and B according to the presence or absence of varnish protection. The eight subgroup samples were stored in distilled water for 30 days and received the following treatments for 14 days: G2A: varnish protection and immersion in soft drink, G2B: no varnish protection and immersion in soft drink, G3A: varnish protection and immersion in orange juice, G3B: no varnish protection and immersion in orange juice, G4A: varnish protection and immersion in yogurt, G4B: no varnish protection and immersion in yogurt. The immersion procedure was performed three times a day, for 15 minutes at a time, for a total of 14 days. The samples from subgroups G1A (with varnish) and G1B (without varnish) were used as controls and stored in distilled water only for 30 days. The samples were submitted to a compressive strength test after the immersion period. The results were analyzed using the ANOVA 2, Tukey test (5%) and Student’s t-test (5%). There were no significant differences between the subgroups, except for the subgroup with varnish protection and immersion in orange juice, which showed reduced GIC compressive strength.


e-GIGI ◽  
2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Vanessa M. Roeroe ◽  
Dinar A. Wicaksono ◽  
Juliatri .

Abstract: Glass ionomer cement is restorative materials that attached on the enamel and dentin through chemical combination, that consists of powder and liquid. GIC powder and liquid are alumino silikat glass and from poliakrilik acid. Characters of GIC are biocompatible, removing fluoride, prevent developing cariest, esthetic, translucent, low solubility, anti bacterial. Nowadays, aperitif that consumed by people has sour nature and solvent. The purpose of this research is to know description compressive strength transformation of type II GIC that soaked in variant percentage of aperitif. This was an experimental research used post test only control group design. Each samples were 5 for every treat. Sample consisted of Type II SIK materials which soaked during 24 hours in 5%, 10%, 40% aperitif, and aquades as control. Endurance and Strength of Type II GIC measured by compression test device. From research result available that mean of strength GIC measurement which soaked in aperitif 5%, 10%, 40%, aquades such as 2,84 MPa, 3,36 MPa, 4,5 MPa, and 2,58 MPa as control variable. Description of this research is transformation of compressive strength GIC increasing if soaked in higher percentage aperitif because sample appication was not yet perfect.Keywords: compressive strength, glass ionomer cement, aperitifAbstrak: Semen ionomer kaca adalah bahan restorasi yang melekat pada enamel dan dentin melalui ikatan kimia, terdiri dari campuran powder dan liquid. PowderSIK adalah kaca aluminosilikat dan liquid larutan asam poliakrilik. Beberapa sifat yang dimiliki semen ionomer kaca yaitu biokompatibilitas melepas fluor sehingga dapat mencegah karies lebih lanjut, estetis, daya larut rendah, translusen, dan bersifat anti bakteri. Minuman beralkohol yang banyak dikonsumsi masyarakat saat ini mempunyai sifat asam dan sebagai pelarut. Tujuan penelitian ini untuk mengetahui gambaran perubahan kekuatan tekan bahan tumpatan SIK tipe II yang direndam dalam minuman beralkohol dengan berbagai presentase. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Masing-masing sampel dibuat 5 buah untuk setiap perlakuan. Sampel terdiri atas bahan SIK tipe II yang direndam dengan variasi presentase 5%, 10%, 40% alkohol dan aquades sebagai kontrol selama 24 jam. Kekuatan tekan SIK diukur menggunakan alat tes kompresi. Berdasarkan hasil penelitian didapatkan rata-rata pengukuran kekuatan SIK yang direndam dalam alkohol 5%, 10%, 40%, dan kontrol aquades masing-masing 2,84 MPa, 3,36 MPa, 4,5 MPa, 2,58 MPa. Gambaran dari hasil penelitian ini bahwa perubahan kekuatan tekan SIK semakin meningkat apabila direndam dalam kadar minuman beralkohol lebih tinggi karena proses pembuatan sampel SIK belum sempurna.Kata kunci: kekuatan tekan, semen ionomer kaca, minuman beralkohol


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