EFFECT OF ADDING EQUEX STM PASTE AND BHT TO DIFFERENT EXTENDERS ON VIABILITY, PLASMA MEMBRANE AND DNA INTEGRITY OF GERMAN SHEPHERD DOG SPERMATOZOA DURING FREEZING AND THAWING METHODS IN EGYPT

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

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Czechoslovakian Wolfdog Genomic Divergence from Its Ancestors Canis lupus, German Shepherd Dog, and Different Sheepdogs of European Origin

Genes ◽

10.3390/genes12060832 ◽

2021 ◽

Vol 12 (6) ◽

pp. 832

Author(s):

Nina Moravčíková ◽

Radovan Kasarda ◽

Radoslav Židek ◽

Luboš Vostrý ◽

Hana Vostrá-Vydrová ◽

...

Keyword(s):

Demographic History ◽

Genome Structure ◽

Principal Component ◽

Genetic Distances ◽

Nucleotide Polymorphisms ◽

German Shepherd ◽

German Shepherd Dog ◽

Genome Wide ◽

Scale Population ◽

History Effect

This study focused on the genomic differences between the Czechoslovakian wolfdog (CWD) and its ancestors, the Grey wolf (GW) and German Shepherd dog. The Saarloos wolfdog and Belgian Shepherd dog were also included to study the level of GW genetics retained in the genome of domesticated breeds. The dataset consisted of 131 animals and 143,593 single nucleotide polymorphisms (SNPs). The effects of demographic history on the overall genome structure were determined by screening the distribution of the homozygous segments. The genetic variance distributed within and between groups was quantified by genetic distances, the FST index, and discriminant analysis of principal components. Fine-scale population stratification due to specific morphological and behavioural traits was assessed by principal component and factorial analyses. In the CWD, a demographic history effect was manifested mainly in a high genome-wide proportion of short homozygous segments corresponding to a historical load of inbreeding derived from founders. The observed proportion of long homozygous segments indicated that the inbreeding events shaped the CWD genome relatively recently compared to other groups. Even if there was a significant increase in genetic similarity among wolf-like breeds, they were genetically separated from each other. Moreover, this study showed that the CWD genome carries private alleles that are not found in either wolves or other dog breeds analysed in this study.

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Massive attack by honeybees in a German shepherd dog: description of a fatal case and review of the literature

Journal of Venomous Animals and Toxins including Tropical Diseases ◽

10.1186/1678-9199-20-55 ◽

2014 ◽

Vol 20 (1) ◽

pp. 55 ◽

Cited By ~ 3

Author(s):

Mudassar Mughal ◽

Ghazanfar Abbas ◽

Muhammad Saqib ◽

Ghulam Muhammad

Keyword(s):

Fatal Case ◽

Review Of The Literature ◽

German Shepherd ◽

German Shepherd Dog

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Disseminated Scytalidium infection in a German shepherd dog

Medical Mycology Case Reports ◽

10.1016/j.mmcr.2015.01.001 ◽

2015 ◽

Vol 7 ◽

pp. 20-22 ◽

Cited By ~ 5

Author(s):

Anna E. Dunlap ◽

Amy K. Swinford ◽

Katherine L. Wells

Keyword(s):

German Shepherd ◽

German Shepherd Dog

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Progesterone treatment of boar spermatozoa improves male pronuclear formation after intracytoplasmic sperm injection into porcine oocytes

Zygote ◽

10.1017/s0967199402002137 ◽

2002 ◽

Vol 10 (2) ◽

pp. 95-104 ◽

Cited By ~ 16

Author(s):

Mike Katayama ◽

Takashi Miyano ◽

Masashi Miyake ◽

Seishiro Kato

Keyword(s):

Plasma Membrane ◽

Intracytoplasmic Sperm Injection ◽

Acrosome Reaction ◽

Sperm Chromatin ◽

Oocyte Activation ◽

Freezing And Thawing ◽

Chromatin Decondensation ◽

Progesterone Treatment ◽

Pronuclear Formation ◽

Boar Spermatozoa

Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation of oocytes and the transformation of the sperm nucleus following ICSI were assessed. After freezing and thawing, the plasma membrane and acrosomal contents over the apical region of sperm head were lost in all the spermatozoa. Following treatment with 1 mg/ml progesterone, the acrosome reaction was induced in 61% of spermatozoa. After injection of three types of spermatozoa, non-treated spermatozoa and progesterone-treated (i.e. acrosome-reacted) spermatozoa induced oocyte activation, but frozen-thawed spermatozoa induced oocyte activation at a significantly lower rate. Sixty-two per cent of sperm heads remained orcein-negative for 6 h, however, resulting in delayed sperm chromatin decondensation and low male pronuclear formation in the oocytes injected with a non-treated spermatazoon. Since the treatments of freezing and thawing and progesterone for spermatozoa accelerated the initial change in sperm chromatin and the latter treatment induced oocyte activation earlier, it is considered that the delay in oocyte activation and decondensation of sperm chromatin after injection of non-treated spermatozoa is caused by the existence of the sperm plasma membrane. These results show that progesterone treatment efficiently induces the acrosome reaction in boar spermatozoa without destroying their potency for oocyte activation, and the induction of the acrosome reaction results in the promotion of male pronuclear formation after ICSI.

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Corneal pannus (chronic superficial keratitis) in the German Shepherd Dog

Journal of Small Animal Practice ◽

10.1111/j.1748-5827.1979.tb07019.x ◽

1979 ◽

Vol 20 (1) ◽

pp. 41-56 ◽

Cited By ~ 21

Author(s):

P. G. C. BEDFORD ◽

J. A. LONGSTAFFE

Keyword(s):

German Shepherd ◽

German Shepherd Dog

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Diffuse annular fusiform adenocarcinoma in a dog

Journal of the American Animal Hospital Association ◽

10.5326/15473317-36-2-169 ◽

2000 ◽

Vol 36 (2) ◽

pp. 169-173 ◽

Cited By ~ 10

Author(s):

MR Prater ◽

B Flatland ◽

SJ Newman ◽

DP Sponenberg ◽

J Chao

Keyword(s):

Large Bowel ◽

Unusual Case ◽

Clinical Signs ◽

Postmortem Examination ◽

Watery Diarrhea ◽

Colonic Adenocarcinoma ◽

German Shepherd ◽

Regional Lymph Nodes ◽

German Shepherd Dog ◽

Neoplastic Lesion

Canine colonic intestinal adenocarcinoma typically presents as rectal polypoid or annular stenotic masses causing clinical signs consistent with large bowel disease. This report discusses an unusual case of intestinal adenocarcinoma in an 11-year-old, neutered male German shepherd dog presented for evaluation of anorexia, profuse watery diarrhea, and weight loss. In this dog, colonic adenocarcinoma diffusely infiltrated the entire large bowel and caused an annular fusiform lesion, as confirmed by endoscopic biopsies and postmortem examination. Other unique features included a paucity of desmoplasia associated with the neoplastic lesion and widespread metastasis to regional lymph nodes, lung, and prostate.

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Transient hyperammonaemia in an adult German shepherd dog : case report

Journal of the South African Veterinary Association ◽

10.4102/jsava.v68i2.873 ◽

1997 ◽

Vol 68 (2) ◽

Cited By ~ 3

Author(s):

R.G. Lobetti ◽

D.B. Miller ◽

T. Dippenaar

Keyword(s):

Case Report ◽

Liver Disease ◽

Urinary Tract ◽

Drug Therapy ◽

Urea Cycle ◽

Urinary Tract Obstruction ◽

German Shepherd ◽

Cycle Enzyme ◽

German Shepherd Dog ◽

Urea Cycle Enzyme

A 3-year-old male German shepherd dog was presented with severe generalised seizures. The dog was protein-intolerant and showed severe hyperammonaemia on ammonia stimulation. The hyperammonaemic state was present for at least 6 weeks and then spontaneously resolved. No obvious cause (liver disease, portocaval shunts, urea cycle enzyme deficiencies, drug therapy or urinary tract obstruction) could be identified. It is possible that this dog had a variation of transient hyperammonaemic syndrome, described in man and recently in a juvenile Irish wolfhound, that extended into adulthood.

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