Effects Of Mono-Sodium Glutamate Administration On Metabolic Parameters, Hepatic And Renal Functions In Adult And Neonate Male Rats

Background: Obesity is because of excessive fat accumulation that affects health adversely in the form of various diseases such as diabetes, hypertension, cardiovascular diseases, and many other disorders. Our Indian diet is rich in carbohydrates, and hence the sucrose-induced obesity is an apt model to mimic this. Ventromedial hypothalamus (VMH) is linked to the regulation of food intake in animals as well as humans. Purpose: To understand the role of VMHin sucrose-induced obesity on metabolic parameters. Methods: A total of 24 adult rats were made obese by feeding them on a 32% sucrose solution for 10 weeks. The VMH nucleus was ablated in the experimental group and sham lesions were made in the control group. Food intake, body weight, and biochemical parameters were compared before and after the lesion. Results: Male rats had a significant weight gain along with hyperphagia, whereas female rats did not have a significant weight gain inspite of hyperphagia. Insulin resistance and dyslipidemia were seen in both the experimental and control groups. Conclusion: A sucrose diet produces obesity which is similar to the metabolic syndrome with insulin resistance and dyslipidemia, and a VMH lesion further exaggerates it. Males are more prone to this exaggeration.

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The ameliorative effects of cinnamum zeylanicum extract on renal functions and oxidative stress against fluoxetine drug in male rats

Mansoura Veterinary Medical Journal ◽

10.21608/mvmj.2021.111996.1098 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Nada Hashem ◽

Nabil Abu Heakal ◽

Huda El-Emam ◽

Eman El-Ashry ◽

Mona Elghareeb

Keyword(s):

Oxidative Stress ◽

Male Rats ◽

Renal Functions ◽

And Oxidative Stress

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Adverse Effects of Subchronic Dose of Aspirin on Reproductive Profile of Male Rats

Journal of Pharmaceutics ◽

10.1155/2016/6585430 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Archana Vyas ◽

Heera Ram ◽

Ashok Purohit ◽

Rameshwar Jatwa

Keyword(s):

Adverse Effects ◽

Acetylsalicylic Acid ◽

Serum Biochemistry ◽

Lipid Profiles ◽

Male Rats ◽

Experimental Animals ◽

Renal Functions ◽

Sperm Density ◽

Anti Inflammatory

Aspirin (acetylsalicylic acid) is widely used for cardiovascular prophylaxis and as anti-inflammatory pharmaceutical. An investigation was carried out to evaluate the influence of subchronic dose of aspirin on reproductive profile of male rats, if any. Experimental animals were divided into three groups: control and aspirin subchronic dose of 12.5 mg/kg for 30 days and 60 days, respectively, while alterations in sperm dynamics, testicular histopathological and planimetric investigations, body and organs weights, lipid profiles, and hematology were performed as per aimed objectives. Subchronic dose of aspirin reduced sperm density, count, and mobility in cauda epididymis and testis; histopathology and developing primary spermatogonial cells (primary spermatogonia, secondary spermatogonia, and mature spermatocyte) count were also significantly decreased in rats. Hematological investigations revealed hemopoietic abnormalities in 60-day-treated animals along with dysfunctions in hepatic and renal functions. The findings of the present study revealed that administration with subchronic dose of aspirin to male rats resulted in altered reproductive profiles and serum biochemistry.

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Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α

10.1101/2020.06.02.130674 ◽

2020 ◽

Author(s):

Shivani N. Mann ◽

Niran Hadad ◽

Molly Nelson-Holte ◽

Alicia R. Rothman ◽

Roshini Sathiaseelan ◽

...

Keyword(s):

Estrogen Receptor ◽

Chronic Diseases ◽

Transcriptional Activation ◽

Estrogen Receptor Α ◽

Metabolic Parameters ◽

Metabolic Effects ◽

Male Rats ◽

Male Mice ◽

Dietary Interventions ◽

Estradiol Treatment

ABSTRACTMetabolic dysfunction underlies several chronic diseases, many of which are exacerbated by obesity. Dietary interventions can reverse metabolic declines and slow aging, although compliance issues remain paramount. 17α-estradiol treatment improves metabolic parameters and slows aging in male mice. The mechanisms by which 17α-estradiol elicits these benefits remain unresolved. Herein, we show that 17α-estradiol elicits similar genomic binding and transcriptional activation through estrogen receptor α (ERα) to that of 17β-estradiol. In addition, we show that the ablation of ERα completely attenuates the beneficial metabolic effects of 17α-E2 in male mice. Our findings suggest that 17α-E2 acts primarily through the liver and hypothalamus to improve metabolic parameters in male mice. Lastly, we also determined that 17α-E2 improves metabolic parameters in male rats, thereby proving that the beneficial effects of 17α-E2 are not limited to mice. Collectively, these studies suggest ERα may be a drug target for mitigating chronic diseases in male mammals.

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Effects of a Long-Term Administration of Aqueous Extract of Prunella vulgaris L. on Survival, Spontaneous Thyroid Carcinoma and Neoplastic C-cell Hyperplasia in Rats

10.21203/rs.3.rs-96133/v1 ◽

2020 ◽

Author(s):

Jia Song ◽

Min Zhao ◽

Zhengbao Zhang ◽

Jianbin Tan ◽

Bifeng Chen ◽

...

Keyword(s):

Thyroid Carcinoma ◽

Aqueous Extract ◽

Metabolic Parameters ◽

Male Rats ◽

Preneoplastic Lesion ◽

Prunella Vulgaris ◽

Cell Hyperplasia ◽

C Cell Hyperplasia ◽

Term Administration

Abstract Background: The continued global rise in thyroid carcinoma calls for alternative prevention and treatment strategies. Prunella vulgaris L. (PV) is a herbaceous plant with a medicinal property in the treatment of thyroid gland dysfunction, but its influence on thyroid carcinoma is unclear so far. This study was designed to investigate the effects of aqueous extract of PV on survival, spontaneous thyroid carcinoma and its preneoplastic lesion in rats.Methods: A total of 552 Wistar rats (half female and half male) were randomly assigned into 4 groups and given one of the following diets for 24 months: chow diet (control), 2.5 (low), 8.25 (middle) and 25 (high) g/kg bw PV diets. After intervention, serum metabolic parameters including indicators of liver and renal function, glucose and lipid profiles were measured. Histological examination was conducted to confirm the types of thyroid carcinoma and its preneoplastic lesion. Results: After intervention, serum aspartate transaminase of male rats in high PV group decreased significantly. No statistical differences among groups in terms of survival, body weight and other metabolic parameters were detected. In the control, low, middle and high PV groups, 14, 14, 15 and 8 rats developed thyroid carcinoma, respectively. Medullary thyroid carcinoma (MTC) emerged as the most common histological type in both sexes. Although PV failed to decrease risk of total thyroid carcinoma or each histological type, the incidence rates of neoplastic C-cell hyperplasia (CCH, a preneoplastic lesion of hereditary MTC) in PV groups were lower than that of control, and the lowest was observed in high PV group, manifesting as 5.25-time decrease in female rats and 5.5-time decrease in male rats.Conclusion: Our results suggested for the first time that, a long-term administration of aqueous extract of PV decreased the incidence of neoplastic CCH without impairing survival and metabolic parameters.

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Effects of indole-3-carbinol on metabolic parameters and on lipogenesis and lipolysis in adipocytes 182

Czech Journal of Animal Science ◽

10.17221/1745-cjas ◽

2009 ◽

Vol 54 (No. 4) ◽

pp. 182-189 ◽

Cited By ~ 3

Author(s):

M. Okulicz ◽

I. Hertig ◽

J. Chichłowska

Keyword(s):

Metabolic Pathways ◽

Direct Action ◽

Liver Steatosis ◽

Density Lipoprotein ◽

Metabolic Parameters ◽

Male Rats ◽

Liver Cholesterol ◽

Carbohydrate And Lipid Metabolism

: Indole-3-carbinol (I3C) was found to have possible anticarcinogenic, antioxidant and anti-atherogenic effects on the organism. So far, its influence on metabolic pathways has been unknown. This work was the first attempt to determine the carbohydrate and lipid metabolism changes in vivo after administration of 150 mg/kg b.wt./day I3C to male rats. Additionally, the aim of this trial was to evaluate the direct effect of I3C on basal and hormone-induced lipogenesis and lipolysis in isolated rat adipocytes at concentrations 1, 10, 100 μM in vitro. We can corroborate that adipocytes are susceptible to the direct action of I3C. The incubation of adipocytes with I3C at the three above-mentioned concentrations resulted in its influence on restriction of glucose entry into adipocytes in the basal as well as insulin-stimulated conditions. However, it was observed that I3C at these concentrations strongly intensified basic and epinephrine-stimulated lipolysis. I3C also has a significant influence on metabolism in vivo. Its administration to rats caused a significant increase in the content of triglycerides and a decrease in glycogen in the liver. The considerable augmentation of glucose, triglycerides, cholesterol in high-density lipoprotein and insulin with a concomitant decrease in FFA concentrations was noted in the blood serum. I3C did not alter phospholipids, total, free, esterified cholesterol in the serum and the liver cholesterol. The results obtained in vivo and in vivo indicate that the effect of I3C is adverse for the majority of metabolic parameters which were investigated. The most important finding in this study is the effect of I3C on liver steatosis and that the observed lower lipogenesis at higher lipolysis in fat cells may be involved in the mechanism.

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Decreased Sex Hormone-Binding Globulin Indicated Worse Biometric, Lipid, Liver, and Renal Function Parameters in Women with Polycystic Ovary Syndrome

International Journal of Endocrinology ◽

10.1155/2020/7580218 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Xi Luo ◽

Xin-Ming Yang ◽

Wang-Yu Cai ◽

Hui Chang ◽

Hong-Li Ma ◽

...

Keyword(s):

Blood Pressure ◽

Metabolic Syndrome ◽

Renal Function ◽

Polycystic Ovary ◽

Density Lipoprotein ◽

Metabolic Parameters ◽

Sex Hormone Binding Globulin ◽

Hormone Binding ◽

Ovary Syndrome ◽

Renal Functions

Objective. To investigate the relationships between sex hormone-binding globulin (SHBG) and comprehensive metabolic parameters including biometric, glycemic, lipid, liver, and renal functions of women with polycystic ovary syndrome (PCOS). Study Design and Methods. A total of 1000 women diagnosed as PCOS by modified Rotterdam criteria were enrolled in a randomized controlled trial. SHBG and comprehensive metabolic parameters were measured at the baseline visit. Metabolic parameters included biometric parameters, glucose and lipid panels, and liver and renal function parameters. An independent t-test and linear regression were performed to investigate the associations between SHBG and metabolic parameters. Logistic regression was used to detect the relationship between SHBG and the presence of metabolic syndrome. Results. In comparative analyses, PCOS women with lower SHBG levels had higher body mass index, waist circumference, insulin, homeostatic model assessment-insulin resistance (HOMA-IR) index, systolic and diastolic blood pressure, triglycerides, apolipoprotein B (APOB), low-density lipoprotein (LDL), aspartate transferase (AST), alanine transferase (ALT), and blood urea nitrogen (BUN), but lower high-density lipoprotein (HDL) and apolipoprotein A1 (APOA1). In linear regression, SHBG was inversely associated with waist circumference, systolic blood pressure, triglyceride, LDL, APOB, ALT, AST, and BUN but positively associated with HDL and APOA1 after adjusting the BMI. In logistic regression, SHBG is a protective predictor for metabolic syndrome (odds ratio = 0.96; 95% confidence interval: 0.95–0.97). The area under the receiver-operator characteristic curve is 0.732 with a 95% confidence interval of 0.695–0.770. SHBG <26.75 mmol/L is the cutoff point with the best Youden index, which has a sensitivity of 0.656 and specificity of 0.698. Conclusions. Lower SHBG was associated with worsening biometric, lipid, liver, and renal functions but not glycemic parameters among women with PCOS. SHBG can be used as a tool to screen metabolic syndrome. This trial is registered with NCT01573858 and ChiCTR-TRC-12002081.

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Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α

eLife ◽

10.7554/elife.59616 ◽

2020 ◽

Vol 9 ◽

Author(s):

Shivani N Mann ◽

Niran Hadad ◽

Molly Nelson Holte ◽

Alicia R Rothman ◽

Roshini Sathiaseelan ◽

...

Keyword(s):

Estrogen Receptor ◽

Chronic Diseases ◽

Transcriptional Activation ◽

Estrogen Receptor Α ◽

Metabolic Parameters ◽

Metabolic Effects ◽

Male Rats ◽

Male Mice ◽

Dietary Interventions ◽

Estradiol Treatment

Metabolic dysfunction underlies several chronic diseases, many of which are exacerbated by obesity. Dietary interventions can reverse metabolic declines and slow aging, although compliance issues remain paramount. 17α-estradiol treatment improves metabolic parameters and slows aging in male mice. The mechanisms by which 17α-estradiol elicits these benefits remain unresolved. Herein, we show that 17α-estradiol elicits similar genomic binding and transcriptional activation through estrogen receptor α (ERα) to that of 17β-estradiol. In addition, we show that the ablation of ERα completely attenuates the beneficial metabolic effects of 17α-E2 in male mice. Our findings suggest that 17α-E2 may act through the liver and hypothalamus to improve metabolic parameters in male mice. Lastly, we also determined that 17α-E2 improves metabolic parameters in male rats, thereby proving that the beneficial effects of 17α-E2 are not limited to mice. Collectively, these studies suggest ERα may be a drug target for mitigating chronic diseases in male mammals.

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Testosterone deprivation accelerates cardiac dysfunction in obese male rats

Journal of Endocrinology ◽

10.1530/joe-16-0002 ◽

2016 ◽

Vol 229 (3) ◽

pp. 209-220 ◽

Cited By ~ 17

Author(s):

Wanpitak Pongkan ◽

Hiranya Pintana ◽

Sivaporn Sivasinprasasn ◽

Thidarat Jaiwongkam ◽

Siriporn C Chattipakorn ◽

...

Keyword(s):

Insulin Resistance ◽

Mitochondrial Function ◽

Cardiac Dysfunction ◽

Left Ventricular ◽

Normal Diet ◽

Metabolic Parameters ◽

Male Rats ◽

Lv Function ◽

Insulin Resistant ◽

Aging Men

Low testosterone level is associated with increased risks of cardiovascular diseases. As obese-insulin-resistant condition could impair cardiac function and that the incidence of obesity is increased in aging men, a condition of testosterone deprivation could aggravate the cardiac dysfunction in obese-insulin-resistant subjects. However, the mechanism underlying this adverse effect is unclear. This study investigated the effects of obesity on metabolic parameters, heart rate variability (HRV), left ventricular (LV) function, and cardiac mitochondrial function in testosterone-deprived rats. Orchiectomized or sham-operated male Wistar rats (n=36per group) were randomly divided into groups and were given either a normal diet (ND, 19.77% of energy fat) or a high-fat diet (HFD, 57.60% of energy fat) for 12weeks. Metabolic parameters, HRV, LV function, and cardiac mitochondrial function were determined at 4, 8, and 12weeks after starting each feeding program. We found that insulin resistance was observed after 8weeks of the consumption of a HFD in both sham (HFS) and orchiectomized (HFO) rats. Neither the ND sham (NDS) group nor ND orchiectomized (NDO) rats developed insulin resistance. The development of depressed HRV, LV contractile dysfunction, and increased cardiac mitochondrial reactive oxygen species production was observed earlier in orchiectomized (NDO and HFO) rats at week 4, whereas HFS rats exhibited these impairments later at week 8. These findings suggest that testosterone deprivation accelerates the impairment of cardiac autonomic regulation and LV function via increased oxidative stress and impaired cardiac mitochondrial function in obese-orchiectomized male rats.

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