scholarly journals Evaluation of serum folate level before and after oral PUVA therapy in psoriatic patients

2021 ◽  
Vol 6 (2) ◽  
pp. 173-177
Author(s):  
A.A. Ibrahim ◽  
S.E. Ibrahim ◽  
R.T. Shafik
Keyword(s):  
Serum Folate ◽  
Folate Level ◽  
Puva Therapy ◽  
Serum Folate Level ◽  
Before And After

Evaluation of serum folate level before and after bath PUVA therapy in patients referred to Razi Hospital, Rasht, Iran

2019 ◽  
Vol 32 (5) ◽  
Author(s):  
Leila Yousefkhani ◽  
Rana Rafiei ◽  
Abbas Darjani ◽  
Narges Alizadeh ◽  
Hojat Eftekhari ◽  
...  
Keyword(s):  
Serum Folate ◽  
Folate Level ◽  
Puva Therapy ◽  
Serum Folate Level ◽  
Before And After

scholarly journals Clinical and laboratory observations on serum folate-binding protein

Blood ◽  
1975 ◽  
Vol 46 (4) ◽  
pp. 599-609 ◽  
Author(s):  
ER Eichner ◽  
CJ Paine ◽  
VL Dickson ◽  
MD Jr Hargrove
Keyword(s):  
Binding Protein ◽  
Elevated Serum ◽  
Normal Subjects ◽  
Serum Levels ◽  
Serum Folate ◽  
Folate Level ◽  
Folate Binding Protein ◽  
Serum Folate Level ◽  
Relationship Of ◽  
The Relationship

Abstract We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some women taking oral contraceptives, and in most patients with uremia. Elevated levels of unsaturated FBP will produce falsely low results in folate radioassay unless the FBP has been destroyed by heat, as was done in the modified radioassay here presented. In normal and uremic subjects, serum folate and unsaturated FBP levels tended to correlate, whereas in patients taking large doses of folic acid the level of unsaturated FBP fell as the level of serum folate rose.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

Abstract The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

Pre-treatment serum folate levels and toxicity in colorectal cancer patients treated with 5-fluorouracil and folinic acid.

2016 ◽  
Vol 34 (4_suppl) ◽  
pp. 755-755 ◽  
Author(s):  
Michael Yan ◽  
Mark David Vincent ◽  
Cheryl Ho ◽  
Eric Winquist ◽  
Derek J. Jonker ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Folinic Acid ◽  
Cancer Patients ◽  
Serum Folate ◽  
Folate Level ◽  
Serum Folate Level ◽  
Pre Treatment ◽  
Grade 3 ◽  
Colorectal Cancer Patients ◽  
Schedule Modification

755 Background: 5-fluorouracil (5-FU) chemotherapy is associated with severe and unpredictable toxicity in a significant proportion of patients.We hypothesized that susceptibility to 5-FU toxicity might be related to individual differences in components of reduced folate metabolism affecting intracellular 5,10-MTHF levels, detectable in blood prior to treatment. Methods: A prospective cohort of chemo-naive colorectal cancer patients planned to receive IV 5-FU and folinic acid for five consecutive days every four weeks were studied. Baseline clinical and laboratory data were collected prior to treatment. Biochemical data associated with folate metabolism was also collected and not revealed to treating physicians. The primary endpoint was occurrence of > grade 3 toxicity and/or toxicity mandating dose delay or reduction. Results: Of 78 eligible patients studied, 68% experienced > grade 3 toxicity, 69% had schedule modification, and 81% had either or both. Multivariable analyses identified only a higher pre-treatment serum folate level as an independent predictor of toxicity > grade 3 and/or mandating schedule modification (p = 0.016). An increasing toxicity trend was observed amongst folate-stratified patient cohorts, with an odds ratio of 2.87 (p = 0.09) comparing the highest and lowest quartiles. Concurrently, overall survival and relapse-free rates also increased with pretreatment folate levels in the adjuvant cohort with log rank values of 3.60 (p = 0.06) and 7.20 (p = 0.007) between the highest quartile and the lower quartiles, respectively. Conclusions: The incidence of severe toxicity with this schedule of 5-FU and folinic acid was high and positively correlated with the pretreatment serum folate level. Interestingly, a concurrent increase in overall and relapse free survival is observed with increasing serum folate levels. These results suggest that an optimal pretreatment folate level exists that balances chemotherapy treatment efficacy and toxicity.

ORAL CONTRACEPTIVES AND SERUM-FOLATE LEVEL

The Lancet ◽  
1968 ◽  
Vol 291 (7556) ◽  
pp. 1376-1377 ◽  
Author(s):  
A Majid Shojania ◽  
Gloria Hornady ◽  
PhilipH Barnes
Keyword(s):  
Oral Contraceptives ◽  
Serum Folate ◽  
Folate Level ◽  
Serum Folate Level

New Tween-80 Microbiological Assay of Serum Folate Levels in Humans and Animals

2012 ◽  
Vol 95 (5) ◽  
pp. 1505-1510 ◽  
Author(s):  
Zhenghua Zhou ◽  
Yuan Yang ◽  
Ming Li ◽  
Chong Kou ◽  
Ping Xiao ◽  
...  
Keyword(s):  
Folic Acid ◽  
Tween 80 ◽  
Microbiological Assay ◽  
Serum Folate ◽  
Optimal Conditions ◽  
Folate Level ◽  
Minimum Concentration ◽  
Control Subjects ◽  
Serum Folate Level ◽  
Healthy Control

Abstract The objective of this study was to develop a new Tween-80 microbiological assay (Tween-80 MBA) to determine human or animal serum folate levels and to verify its reliability. The effects of the Lactobacillius casei subspecies rhamnosus (L. casei, ATCC No. 7469) inoculum concentration, incubation time, and Tween-80 on L. casei growth were studied, and the serum folate levels were investigated. Serum samples were collected from patients with esophageal cancer (EC) and healthy control subjects in Yanting, healthy adult subjects in Chengdu, Sichuan, and in male Sprague-Dawley rats. Optimal conditions for the new MBA were as follows: 1.28 × 107 CFU/mL working inoculum, vitamin folic acid assay broth with 0.24% (w/w) Tween-80, and anaerobic incubation with L. casei at 37°C for 22 h. Under the optimal conditions, the working curve was in simple linear rather than logarithmic equation; the linear working curve of the folic acid standard working solution concentration versus the turbidity (adsorption value) of medium with L. casei ranged from 0.05 to 1.00 μg/L; the linear correlation coefficient was 0.9989 (SD 0.0007); the recovery rate of folate was 105.4–112.7%; and the minimum concentration for detecting folate was 0.03 μg/L. The RSD within-day and between-day precisions were 5.6 and 3.3%, respectively. The serum folate level of 100 EC patients was 6.4 (SEM 0.4) μg/L which was significantly lower than that of healthy control subjects [8.0 (SEM 0.6) μg/L, n = 100, P = 0.020]. The new Tween-80 MBA is considered to be a reliable method for measuring serum folate level.

scholarly journals Serum folate levels in end stage renal disease patients

2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Ghazanfar Ali Sirhindi ◽  
Saadia Shahzad Alam ◽  
Shahida Amjad Riaz Shah ◽  
Javed Asif ◽  
Mateen Izhar ◽  
...  
Keyword(s):  
Renal Disease ◽  
End Stage Renal Disease ◽  
Serum Folate ◽  
Control Group ◽  
Folate Level ◽  
Healthy Individuals ◽  
Serum Folate Level ◽  
Group B ◽  
Stage Renal Disease ◽  
End Stage

Objective: This study was aimed to measure serum folate levels in patients of end stage renal disease (ESRD) on regular haemodialysis and compared with normal healthy individuals. Study design: It is a comparative study. Setting: Department of Pathology, Shaikh Zayed Hospital, Lahore Patients and methods: One hundred subjects were selected and divided into two groups. Group A include fifty patients of end stage renal disease on regular haemodialysis and group B include fifty normal healthy individuals as control. Two ml of blood was drawn by venepuncture, one ml for complete blood examination and one ml for serum folate level. Results: A significant decrease in folate level was observed in end stage renal disease patients on regular haemodialysis as compared to control group. Conclusion: By measuring serum folate level in patients on regular haemodialysis we can manage anaemia due to folate deficiency.

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