New Tween-80 Microbiological Assay of Serum Folate Levels in Humans and Animals

2012 ◽  
Vol 95 (5) ◽  
pp. 1505-1510 ◽  
Author(s):  
Zhenghua Zhou ◽  
Yuan Yang ◽  
Ming Li ◽  
Chong Kou ◽  
Ping Xiao ◽  
...  
Keyword(s):  
Folic Acid ◽  
Tween 80 ◽  
Microbiological Assay ◽  
Serum Folate ◽  
Optimal Conditions ◽  
Folate Level ◽  
Minimum Concentration ◽  
Control Subjects ◽  
Serum Folate Level ◽  
Healthy Control

Abstract The objective of this study was to develop a new Tween-80 microbiological assay (Tween-80 MBA) to determine human or animal serum folate levels and to verify its reliability. The effects of the Lactobacillius casei subspecies rhamnosus (L. casei, ATCC No. 7469) inoculum concentration, incubation time, and Tween-80 on L. casei growth were studied, and the serum folate levels were investigated. Serum samples were collected from patients with esophageal cancer (EC) and healthy control subjects in Yanting, healthy adult subjects in Chengdu, Sichuan, and in male Sprague-Dawley rats. Optimal conditions for the new MBA were as follows: 1.28 × 107 CFU/mL working inoculum, vitamin folic acid assay broth with 0.24% (w/w) Tween-80, and anaerobic incubation with L. casei at 37°C for 22 h. Under the optimal conditions, the working curve was in simple linear rather than logarithmic equation; the linear working curve of the folic acid standard working solution concentration versus the turbidity (adsorption value) of medium with L. casei ranged from 0.05 to 1.00 μg/L; the linear correlation coefficient was 0.9989 (SD 0.0007); the recovery rate of folate was 105.4–112.7%; and the minimum concentration for detecting folate was 0.03 μg/L. The RSD within-day and between-day precisions were 5.6 and 3.3%, respectively. The serum folate level of 100 EC patients was 6.4 (SEM 0.4) μg/L which was significantly lower than that of healthy control subjects [8.0 (SEM 0.6) μg/L, n = 100, P = 0.020]. The new Tween-80 MBA is considered to be a reliable method for measuring serum folate level.

Effect of folic acid intervention on the change of serum folate level in hypertensive Chinese adults

2012 ◽  
Vol 22 (6) ◽  
pp. 421-428 ◽  
Author(s):  
Xianhui Qin ◽  
Jianping Li ◽  
Yimin Cui ◽  
Zeyuan Liu ◽  
Zhigang Zhao ◽  
...  
Keyword(s):  
Folic Acid ◽  
Serum Folate ◽  
Folate Level ◽  
Chinese Adults ◽  
Serum Folate Level

scholarly journals Effects of Periconceptional Multivitamin Supplementation on Folate and Homocysteine Levels Depending on Genetic Variants of Methyltetrahydrofolate Reductase in Infertile Japanese Women

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1381
Author(s):  
Keiji Kuroda ◽  
Takashi Horikawa ◽  
Yoko Gekka ◽  
Azusa Moriyama ◽  
Kazuki Nakao ◽  
...  
Keyword(s):  
Folic Acid ◽  
Methylenetetrahydrofolate Reductase ◽  
Homocysteine Level ◽  
Folic Acid Supplementation ◽  
Serum Folate ◽  
Folate Level ◽  
Multivitamin Supplementation ◽  
Mthfr Polymorphisms ◽  
Mthfr Genotype ◽  
Multivitamin Supplements

Methylenetetrahydrofolate reductase (MTHFR) has various polymorphisms, and the effects of periconceptional folic acid supplementation for decreasing neural tube defects (NTDs) risk differ depending on the genotypes. This study analyzed the effectiveness of multivitamin supplementation on folate insufficiency and hyperhomocysteinemia, depending on MTHFR polymorphisms. Of 205 women, 72 (35.1%), 100 (48.8%) and 33 (16.1%) had MTHFR CC, CT and TT, respectively. Serum folate and homocysteine levels in women with homozygous mutant TT were significantly lower and higher, respectively, than those in women with CC and CT. In 54 women (26.3% of all women) with a risk of NTDs, multivitamin supplementation containing folic acid and vitamin D for one month increased folate level (5.8 ± 0.9 to 19.2 ± 4.0 ng/mL, p < 0.0001) and decreased the homocysteine level (8.2 ± 3.1 to 5.8 ± 0.8 nmol/mL, p < 0.0001) to minimize the risk of NTDs in all women, regardless of MTHFR genotype. Regardless of MTHFR genotype, multivitamin supplements could control folate and homocysteine levels. Tests for folate and homocysteine levels and optimal multivitamin supplementation in women with risk of NTDs one month or more before pregnancy should be recommended to women who are planning a pregnancy.

scholarly journals Clinical and laboratory observations on serum folate-binding protein

Blood ◽  
1975 ◽  
Vol 46 (4) ◽  
pp. 599-609 ◽  
Author(s):  
ER Eichner ◽  
CJ Paine ◽  
VL Dickson ◽  
MD Jr Hargrove
Keyword(s):  
Binding Protein ◽  
Elevated Serum ◽  
Normal Subjects ◽  
Serum Levels ◽  
Serum Folate ◽  
Folate Level ◽  
Folate Binding Protein ◽  
Serum Folate Level ◽  
Relationship Of ◽  
The Relationship

Abstract We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some women taking oral contraceptives, and in most patients with uremia. Elevated levels of unsaturated FBP will produce falsely low results in folate radioassay unless the FBP has been destroyed by heat, as was done in the modified radioassay here presented. In normal and uremic subjects, serum folate and unsaturated FBP levels tended to correlate, whereas in patients taking large doses of folic acid the level of unsaturated FBP fell as the level of serum folate rose.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

Abstract The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

scholarly journals Association of Serum Folic Acid with Ischemic Stroke

2013 ◽  
Vol 28 (2) ◽  
pp. 74-80
Author(s):  
Hasan Zahidur Rahman ◽  
Md Rafiqul Islam ◽  
Moniruzamman Bhuyian ◽  
Masud Rana ◽  
Rashed Imam ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Folic Acid ◽  
Serum Folate ◽  
Control Group ◽  
Serum Folate Level ◽  
Folic Acid Level ◽  
Serum Folic Acid Level ◽  
The Mean ◽  
And Control ◽  
Serum Folic Acid

Background: Stroke is the third commonest cause of death in developed countries and is responsible for the physical disability of a large population. The study was designed to see the association of serum folic acid with ischemic stroke. It was concluded that the low levels of folic acid are associated with ischemic stroke. Aim and objectives: To evaluate the association of serum folic acid level with ischemic stroke and to measure and compare the serum folic acid level among the cases and control subjects. Material and method: This study was a case-control study which was conducted in the Department of Neurology and Department of Biochemistry of BSMMU, Dhaka, between the period of 1st January 2010 and 31st December 2011 for duration of two years. A total number of 60 patients presented with ischemic stroke and 60 control person were enrolled in this study. All patients of both sexes, aged between 20 years and above presented with ischemic stroke, from 0 day to 1 month that was confirmed by CT scan of head/MRI of brain. Blood sample was collected from the cases and the controls and analyzed at the Dept. of Biochemistry, BSMMU for estimation of serum folic acid, serum homocysteine fasting blood sugar. Fasting lipid profile for cases only. Result: The mean ± SD of age of the cases was 58.42 ± 11.47 and among the cases 45 (75.0%) were male and 15 (25.0%) were female. Among the control male and female were 43 (71.7%) and 17 (28.3%) respectively. The mean (±SD) serum folate level of case and control group was 6.26(± 4.06) and 8.07(± 4.70) respectively. Statistically significant differences was observed between case and control group in term of Serum folate (p<0.05). This study showed serum folate level was deficient at the early period of ischemic stroke. Conclusion: Low serum folate concentration is significantly and independently associated with increased risk for ischemic stroke. DOI: http://dx.doi.org/10.3329/bjn.v28i2.17173 Bangladesh Journal of Neuroscience 2012; Vol. 28 (2): 74-80

Pre-treatment serum folate levels and toxicity in colorectal cancer patients treated with 5-fluorouracil and folinic acid.

2016 ◽  
Vol 34 (4_suppl) ◽  
pp. 755-755 ◽  
Author(s):  
Michael Yan ◽  
Mark David Vincent ◽  
Cheryl Ho ◽  
Eric Winquist ◽  
Derek J. Jonker ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Folinic Acid ◽  
Cancer Patients ◽  
Serum Folate ◽  
Folate Level ◽  
Serum Folate Level ◽  
Pre Treatment ◽  
Grade 3 ◽  
Colorectal Cancer Patients ◽  
Schedule Modification

755 Background: 5-fluorouracil (5-FU) chemotherapy is associated with severe and unpredictable toxicity in a significant proportion of patients.We hypothesized that susceptibility to 5-FU toxicity might be related to individual differences in components of reduced folate metabolism affecting intracellular 5,10-MTHF levels, detectable in blood prior to treatment. Methods: A prospective cohort of chemo-naive colorectal cancer patients planned to receive IV 5-FU and folinic acid for five consecutive days every four weeks were studied. Baseline clinical and laboratory data were collected prior to treatment. Biochemical data associated with folate metabolism was also collected and not revealed to treating physicians. The primary endpoint was occurrence of > grade 3 toxicity and/or toxicity mandating dose delay or reduction. Results: Of 78 eligible patients studied, 68% experienced > grade 3 toxicity, 69% had schedule modification, and 81% had either or both. Multivariable analyses identified only a higher pre-treatment serum folate level as an independent predictor of toxicity > grade 3 and/or mandating schedule modification (p = 0.016). An increasing toxicity trend was observed amongst folate-stratified patient cohorts, with an odds ratio of 2.87 (p = 0.09) comparing the highest and lowest quartiles. Concurrently, overall survival and relapse-free rates also increased with pretreatment folate levels in the adjuvant cohort with log rank values of 3.60 (p = 0.06) and 7.20 (p = 0.007) between the highest quartile and the lower quartiles, respectively. Conclusions: The incidence of severe toxicity with this schedule of 5-FU and folinic acid was high and positively correlated with the pretreatment serum folate level. Interestingly, a concurrent increase in overall and relapse free survival is observed with increasing serum folate levels. These results suggest that an optimal pretreatment folate level exists that balances chemotherapy treatment efficacy and toxicity.

ORAL CONTRACEPTIVES AND SERUM-FOLATE LEVEL

The Lancet ◽  
1968 ◽  
Vol 291 (7556) ◽  
pp. 1376-1377 ◽  
Author(s):  
A Majid Shojania ◽  
Gloria Hornady ◽  
PhilipH Barnes
Keyword(s):  
Oral Contraceptives ◽  
Serum Folate ◽  
Folate Level ◽  
Serum Folate Level
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