scholarly journals Clinical and laboratory observations on serum folate-binding protein

Blood ◽  
1975 ◽  
Vol 46 (4) ◽  
pp. 599-609 ◽  
Author(s):  
ER Eichner ◽  
CJ Paine ◽  
VL Dickson ◽  
MD Jr Hargrove
Keyword(s):  
Binding Protein ◽  
Elevated Serum ◽  
Normal Subjects ◽  
Serum Levels ◽  
Serum Folate ◽  
Folate Level ◽  
Folate Binding Protein ◽  
Serum Folate Level ◽  
Relationship Of ◽  
The Relationship

Abstract We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some women taking oral contraceptives, and in most patients with uremia. Elevated levels of unsaturated FBP will produce falsely low results in folate radioassay unless the FBP has been destroyed by heat, as was done in the modified radioassay here presented. In normal and uremic subjects, serum folate and unsaturated FBP levels tended to correlate, whereas in patients taking large doses of folic acid the level of unsaturated FBP fell as the level of serum folate rose.

scholarly journals Clinical and laboratory observations on serum folate-binding protein

Blood ◽  
1975 ◽  
Vol 46 (4) ◽  
pp. 599-609
Author(s):  
ER Eichner ◽  
CJ Paine ◽  
VL Dickson ◽  
MD Jr Hargrove
Keyword(s):  
Binding Protein ◽  
Elevated Serum ◽  
Normal Subjects ◽  
Serum Levels ◽  
Serum Folate ◽  
Folate Level ◽  
Folate Binding Protein ◽  
Serum Folate Level ◽  
Relationship Of ◽  
The Relationship

We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some women taking oral contraceptives, and in most patients with uremia. Elevated levels of unsaturated FBP will produce falsely low results in folate radioassay unless the FBP has been destroyed by heat, as was done in the modified radioassay here presented. In normal and uremic subjects, serum folate and unsaturated FBP levels tended to correlate, whereas in patients taking large doses of folic acid the level of unsaturated FBP fell as the level of serum folate rose.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

scholarly journals Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy

Blood ◽  
1976 ◽  
Vol 48 (6) ◽  
pp. 911-921 ◽  
Author(s):  
N Colman ◽  
V Herbert
Keyword(s):  
Lactobacillus Casei ◽  
Binding Capacity ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Serum Folate ◽  
Folate Level ◽  
High Affinity ◽  
Serum Folate Level ◽  
Normal Human ◽  
Clinical Conditions

Abstract The current study presents evidence that all human serum contains a class of high-affinity folate binders (KA=2.8 X10(10 liters/mole), which migrate as a single peak on gel filtration. Failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. Direct measurement of the total folate binding capacity (TFBC) has been made possible by dissociation of endogenous folate-binder complexes at acid pH, removal of free folate by coated charcoal, and radiofolate tagging. This procedure does not appear to significantly denature the binders, which release and rebind similar quantities of 3H-PGA. In 20 normal subjects, TFBC ranged from 100 to 325 pg/ml (mean+/-SE = 174+/-16), and was always at least 33% saturated. In three clinical conditions, all associated with elevated unsaturated folate binding capacity, three different patterns emerged when TFBC was also measured. Uremic subjects had significantly elevated mean TFBC with normal saturation. In cirrhotic subjects, mean TFBC approximated normal, but saturation was significantly decreased. In pregnancy, two groups were seen: one with increased TFBC and the other with a normal TFBC, some of whom had decreased saturation. Lactobacillus casei serum folate level was about 30 times greater than the TFBC; there was no correlation between the two measurements.

Is serum folate level a predictor of response in depressive patients who failed to previous antidepressant treatments? a naturalistic study

2011 ◽  
Vol 26 (S2) ◽  
pp. 607-607
Author(s):  
M. Bares ◽  
T. Novak ◽  
M. Kopecek ◽  
P. Stopkova ◽  
C. Höschl
Keyword(s):  
Antidepressant Treatment ◽  
Clinical Status ◽  
Serum Levels ◽  
Response To Treatment ◽  
Serum Folate ◽  
Folate Level ◽  
Baseline Serum ◽  
Madrs Score ◽  
Length Of Treatment ◽  
Serum Folate Level

Introduction and objectiveThe data from previous studies suggested that folate serum level predicts outcome of treatment in patients who failed to respond to SSRI.AimsWe examined the relationship of serum folate level and response to treatment with various antidepressant interventions (antidepressants, combinations of antidepressants and augmentations) in patients who had failed to respond to at least one previous antidepressant treatment.MethodsEighty-four inpatients (mean age = 46.2 ± 11.3 years, 69% woman) with major depression according to DSM IV and without gastrointestinal or other comorbidities that might influenced folate level were enrolled to the study. Clinical status was assessed using MADRS, CGI and BDI-SF at baseline and at the end of study (mean length of treatment- 5.2 ± 0.9 weeks) and response was defined as reduction of MADRS score≥50%. Folate serum levels were determined before start of treatment (normal level >2.5 ng/ml).ResultsLow folate level was detected only in 3 patients (2 responders) in our sample. Responders (54%) and non-responders were not different in baseline folate level as well as in clinical a demographic parameters with exception of number of previous unsuccessful treatments of index episode. Furthermore we did not find any correlations between folate level and baseline or final MADRS score as well as reduction of MADRS respectively.ConclusionDespite encouraging data from previous studies, based on our data we did not suppose association of baseline serum folate level and outcome of treatment in resistant patients.This study was supported by grants of Ministry of Health of Czech Republic NS 10368-3 and MZ0PCP2005.

scholarly journals Higher Serum Phosphorus Is Not an Independent Risk Factor of Mortality in Heart Failure with Reduced Ejection Fraction

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 4004
Author(s):  
Partyka Robert ◽  
Mroczek Alina ◽  
Duda Sylwia ◽  
Malinowska-Borowska Jolanta ◽  
Buczkowska Marta ◽  
...  
Keyword(s):  
Heart Failure ◽  
Elevated Serum ◽  
Serum Levels ◽  
Normal Serum ◽  
Serum Phosphorus ◽  
Prognostic Information ◽  
Reduced Ejection Fraction ◽  
Risk Of Mortality ◽  
Relationship Of ◽  
The Relationship

Higher serum phosphorus has detrimental health effects. Even high-normal rage sP is associated with worse outcomes. The relationship of serum phosphorus with prognostic markers in heart failure remains unclear. We investigated the association of serum phosphorus with heart failure prognostic factors and risk of mortality related to serum phosphorus. In 1029 stable heart failure patients, we investigated the distribution of markers of more advanced heart failure stage across quintiles of serum phosphorus and estimated the relative risk of mortality in comparison to reference. Higher serum phosphorus levels sP were associated with markers of a worse outcome. The best survival was observed in low-normal serum levels. The unadjusted hazard ratio for mortality increased toward higher phosphorus quintiles but not to lower levels of sP. The correction for age, sex, BMI, percent weight loss, inflammation, kidney function, and LVEF did not modify the risk profile substantially. The adjustment for NYHA, natriuretic peptides, serum sodium, and treatment characteristics broke down the risk relationship completely. A higher serum phosphorus is associated with markers of a more risky profile of heart failure. Elevated serum levels of phosphorus sP does not provide independent prognostic information beyond the strongest markers of the severity of the syndrome. The potential involvement of higher serum phosphorus as a mediator in the pathophysiology of heart failure warrants further study.

The Relationship of Root Mean Square Value of Electromyography and Isometric Torque of Quadriceps in Normal Subjects

2016 ◽  
Vol 26 (1) ◽  
pp. 58
Author(s):  
Qiurong XIE ◽  
Zheng JIANG ◽  
Qinglu LUO ◽  
Jie LIANG ◽  
Xiaoling WANG ◽  
...  
Keyword(s):  
Root Mean Square ◽  
Normal Subjects ◽  
Mean Square ◽  
Isometric Torque ◽  
Relationship Of ◽  
The Relationship

scholarly journals Assessment of the relationship of serum liver enzymes activity with general and abdominal obesity in an urban Bangladeshi population

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nurshad Ali ◽  
Abu Hasan Sumon ◽  
Khandaker Atkia Fariha ◽  
Md Asaduzzaman ◽  
Rahanuma Raihanu Kathak ◽  
...  
Keyword(s):  
General Population ◽  
Abdominal Obesity ◽  
Liver Enzymes ◽  
Serum Levels ◽  
General Obesity ◽  
Enzymes Activity ◽  
Elevated Liver Enzymes ◽  
Bangladeshi Population ◽  
Relationship Of ◽  
The Relationship

AbstractObesity is a global health concern because of its increasing trend both in developed and developing countries. A limited number of studies have evaluated the association of liver enzymes with both general and abdominal obesity in the general population; data for the Bangladeshi population are not available yet. This study aimed to assess the relationship of serum liver enzymes activity with both general and abdominal obesity in Bangladeshi adults. In total, 540 blood samples were obtained from the participants (388 males and 152 females) and analyzed for serum levels of ALT, AST, GGT, and ALP using standard methods. General obesity was defined as body mass index (BMI) ≥ 27.5 kg/m2 and abdominal obesity was defined as waist circumference (WC) ≥ 90 cm in males and ≥ 80 cm in females. The relationship between liver enzymes and obesity was evaluated by multivariate logistic regression models. Overall, 58% of participants in the general obesity group and 55% of the participants in the abdominal obesity group had at least one or more elevated levels of liver enzymes. The prevalence of elevated liver enzymes was significantly higher in the obesity group compared to the normal BMI and WC groups (p < 0.05 for all cases). The mean level of serum ALT, AST and GGT were significantly higher in the obesity group than the normal BMI group (p < 0.05). In the WC groups, mean AST and GGT were significantly higher in the obesity group compared to the normal group (p < 0.05). In regression analysis, serum levels of ALT showed an independent and significant association with general obesity, whereas, serum GGT showed a significant association with both general and abdominal obesity. In conclusion, a high prevalence of elevated liver enzymes was observed among participants included in the present study. Of the four enzymes, serum GGT was independently associated with both general and abdominal obesity. Further studies are required to understand the complex relationship between liver enzymes and obesity in the general population.

Sign in / Sign up

Export Citation Format

Share Document