Effects of Moringa Oleifera Aqueous Leaf Extract on Submandibular Salivary Glands of Diabetic Albino Rats

Background: This study was designed to evaluate the effect of Moringa oleifera against Salmonella typhimurium infected changes in liver and kidney function parameters in albino rats.Methods: Twenty acclimatized albino rats were divided into four groups of five rats each (groups A-D). Groups B, C and D were exposed to 0.1ml (3.0x104 CFU) of Salmonella typhimurium. Six hours post- infection, Groups B and C were treated with 50 and 100mg/kg of Moringa oleifera aqueous leaf extract respectively and continued for 21 days. Group D was not treated while group A was neither infected nor treated. Serum alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), bilirubin, albumin, total protein, urea and creatinine were estimated using standard methods. All data were collected and analyzed in October 2015.Results: S. typhimurium infection significantly (p<0.05) increased the levels of ALP, ALT, AST, bilirubin, urea and creatinine when compared with control levels (Group A). Total protein and albumin concentrations were significantly (p<0.05) reduced following the infection. However, Moringa oleifera supplementation was associated with significant (p<0.05) decrease in the levels of ALP, ALT, AST, bilirubin, urea and creatinine. It is also associated with decrease in concentrations of total protein and albumin.Conclusions: This study showed S. typhimurium infection induced changes in liver and kidney function parameters and also revealed possible amelioratory effects to these changes after Moringa oleifera supplementation.

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Protective Effect of N-Acetyl Cysteine on Moringa Oleifera Aqueous Leaf Extract-Induced Hepatic Toxicity in Wistar Albino Rats

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v8i3.749 ◽

2020 ◽

Vol 8 (3) ◽

pp. 34-39

Author(s):

Namaganda Agnes ◽

Kasolo N Josephine ◽

Bbosa S. Godfrey ◽

Lukande Robert ◽

Kimuli Ivan ◽

...

Keyword(s):

Protective Effect ◽

Total Protein ◽

Leaf Extract ◽

Moringa Oleifera ◽

Albino Rats ◽

P Value ◽

Protein Levels ◽

Aqueous Leaf Extract ◽

Acetyl Cysteine ◽

Wistar Albino Rats

Background: Moringa oleifera (M. oleifera) is a commonly used medicinal and nutritive plant. The aqueous leaf extract from this plant contains a high concentration of alkaloids and they are toxic to body organs especially the liver leading to hepatotoxicity after long term exposure. However, if N-Acetyl cysteine (NAC) is administered together with M. oleifera extract, it may have a hepatotoxic protective effect. Objective: To establish the protective effect of N-Acetyl cysteine against M. oleifera aqueous leaf extract-induced hepatotoxicity in the Wistar albino rats. Methods: An experimental laboratory-based study conducted at department of Physiology Makerere University, College of Health Sciences. Three treatment groups of six Wistar albino rats each, were dosed intragastrically once a day for 28 days. Group I; negative control, received 8.05g/kg bwt of M. oleifera extract plus 1ml of normal saline (NS), Group II; test group, received 8.05g/kg bwt of M. oleifera extract plus 50mg/kg of NAC. Group III; positive control, received 750mg/kg bwt of Paracetamol plus 50mg/kg of NAC. On the 14th and 29th day, three animals selected randomly from each group were sacrificed; blood samples were collected, the liver was harvested for histopathological analysis. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and total protein levels were determined. Results: There was an increase in serum ALT, AST and ALP levels in the M. oleifera extract plus NS group which was a sign of hepatotoxicity. The M. oleifera extract plus NAC group showed normal serum ALT, AST and ALP levels with no significant changes in the bilirubin (P-value = 0.9089) and total protein levels (P-value = 0.8858). Conclusion: The results have provided evidence that NAC administration with M. oleifera extract effectively prevents the occurrence of M. oleifera leaves xtract- induced hepatotoxicity.

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