scholarly journals Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis

2020 ◽  
Vol 5 (2) ◽  
pp. 6-12
Author(s):  
Lamees A. El-Tantawy ◽  
Lubna Farahat ◽  
Eman S. Ramadan ◽  
Fatma F. Warda
Keyword(s):  
Rose Bengal ◽  
Bovine Serum ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
Rose Bengal Test

QUANTITATIVE ROSE BENGAL TEST FOR DIAGNOSIS OF BOVINE BRUCELLOSIS

2010 ◽  
Vol 31 (2) ◽  
pp. 120-130 ◽  
Author(s):  
Donghee Cho ◽  
Hyangmi Nam ◽  
Jongwan Kim ◽  
Eunjeong Heo ◽  
Yunsang Cho ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Bovine Brucellosis ◽  
Rose Bengal Test

scholarly journals The use of the milk ring test and rose bengal test in brucellosis control and eradication in Nigeria

2008 ◽  
Vol 79 (3) ◽  
Author(s):  
S.I.B. Cadmus ◽  
H.K. Adesokan ◽  
J. Stack
Keyword(s):  
Rose Bengal ◽  
Screening Tests ◽  
Ring Test ◽  
Serum Samples ◽  
Chi Square ◽  
Southwestern Nigeria ◽  
Rose Bengal Test ◽  
The Difference ◽  
Control And Eradication ◽  
Milk Ring Test

In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61%of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P<0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69%for the MRT and RBT respectively.No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be considered for control and eradication of the disease, especially in Nigeria.

scholarly journals Epidemiology of Bovine Brucellosis by a Combination of Rose Bengal Test and Indirect ELISA in the Five Districts of Uganda

2012 ◽  
Vol 74 (11) ◽  
pp. 1417-1422 ◽  
Author(s):  
Yoshihito KASHIWAZAKI ◽  
Ekisu ECEWU ◽  
Joseph O. IMALIGAT ◽  
Robert MAWEJJE ◽  
Moses KIRUNDA ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Indirect Elisa ◽  
Bovine Brucellosis ◽  
Rose Bengal Test

scholarly journals Comparative Assessment of Sensitivity and Specificity of Rose Bengal Test and Modified In-house ELISA by using IS711 TaqMan Real Time PCR Assay as a Gold Standard for the Diagnosis of Bovine Brucellosis

2018 ◽  
Vol 11 (2) ◽  
pp. 951-957
Author(s):  
Amira M. Zakaria
Keyword(s):  
Positive Predictive Value ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Rose Bengal ◽  
Real Time Pcr ◽  
Predictive Value ◽  
Gold Standard ◽  
Bovine Brucellosis ◽  
Rt Pcr ◽  
Rose Bengal Test

Serological approaches such as Rose Bengal Test (RBT) and enzyme linked immunosorbent assay (ELISA) are common tests, however they are generally not sensitive or specific enough for diagnosis of Brucella because of cross-reactivity with different bacterial antigens. The work objected to evaluate the sensitivity and specificity of rose Bengal and modified in-house ELISA using IS711 real time PCR as a gold test to detect Brucella in calves sera. Two hundred and thirty (n=230) blood samples were collected from (2-3) years asymptomatic male calves in two Egyptian abattoirs. Rose Bengal test (RBT) and modified in-house ELISA were applied to determine the seroprevalence of brucellosis in abattoirs animals while quantitative Taqman real-time PCRs (RT-PCR) were implemented for the identification of Brucella genus. The overall prevalence of brucellosis was (53.9 %), (75.2 %) and (79.1 %) as determined by ELISA,RBT and RT- PCR assays respectively. Regarding statistical analysis of the reported data and considering real time PCR the gold standard, the RBT recorded a sensitivity of (79.12%) and a specificity of (39.58 %) with an accuracy of (70.87%). While (83.24%) was reported as positive predictive value and (33.33 %) as a negative predictive value. The sensitivity and specificity of ELISA were (55.49%) and (52.08 %) respectively while the accuracy was (54.78%). Positive predictive value and negative predictive value for ELISA were determined as (81.45%) and (23.58 %) respectively. RBT can be routinely used for diagnosis of Brucella as cost effective , more sensitive and accurate test than ELISA However, real time PCR is highly recommend as gold test for identification and differentiation of bovine brucellosis.

scholarly journals The Use Of Alternative Stain In Rose Bengal Test Antigen Preparation Which Specific For Brucellosis.

2006 ◽  
Vol 30 (2) ◽  
pp. 56-64
Author(s):  
Erada A .J . Saeed
Keyword(s):  
Serum Sample ◽  
Rose Bengal ◽  
Screening Test ◽  
Serum Samples ◽  
Antigen Preparation ◽  
Specific Antibodies ◽  
Rose Bengal Test ◽  
The Rose ◽  
Food Colours ◽  
Standard Tests

The Rose Bengal test is one of the famous diagnostic test of Brucellosisspecially as a screening test in order to detect the infection in limited area.Rose Bengal standard stain which producd by specific companies was used inpreparation of the test special antigen , The stain gives the known pink colourfor the antigen during the test that make the agglutination in positive cases morevisible due to the reaction between antigen and the specific antibodies ofBrucella which found in the serum sample of human and different animals.Antigen for the Rose Bengal test in this study is prepared by using alternativestain easily found in local supermarkets using for food colours and notexpensive like standard stain. All standard tests were down for the stain like thecolour ,pH, stability are same for two stains until the date of expire of antigen.The antigen prepared with alternative stain was used in comparative with theantigen prepared with standard stain for testing serum samples of human anddifferent animals, the results deal no significant different statistically betweenthem that means as a result we can prepare antigen more easily and notexpensive.

scholarly journals Seroprevalence and Molecular Detection of Bovine Brucellosis

2022 ◽  
Author(s):  
Raghavendra Prasad Mishra ◽  
Udit Jain ◽  
Barkha Sharma ◽  
Kaushal Kusum ◽  
Neha Singh
Keyword(s):  
Serum Sample ◽  
Milk Production ◽  
Bovine Serum ◽  
Human Population ◽  
Molecular Detection ◽  
Specific Gene ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
Livestock Population ◽  
Huge Impact

Background: Brucellosis is one of the major zoonotic problems that exist worldwide. Brucellosis is clinically characterized by metritis, mastitis, repeat breeding, abortion in the last trimester of pregnancy, retention of placenta and reduced milk production in the female whereas epididymitis, orchitis and sterility in male. In humans can be highly variable, ranging from nonspecific, flu-like symptoms to undulant fever, arthritis, orchitis and epididymitis. Methods: A total of 567 bovine serum samples was taken from four districts of Brij region of UP. All the samples were processed to detection of prevalence of brucellosis by RBPT, STAT ELISA and confirmation of genes bcsp31, 16SrRNA, omp2 and IS711 by PCR. Result: The prevalence of brucellosis was found to be 07.93% (31/391), 08.69% (34/391) and 10.74% (42/391) shows positive by RBPT, STAT and I- ELISA respectively. In buffalo Out of 176 tested serum sample the seroprevalence was found to be 09.66% (17/176), 10.79% (19/176) and 12.5% (22/176) positive by RBPT, STAT and I- ELISA respectively. Out of 567 samples 18 were positive for Brucella genus specific gene. The higher prevalence of the disease in this region increases the risk of zoonotic transmission and it implies a serious threat to the human population as well as the huge impact on economy due to loss of productivity as well as loss of livestock population.

scholarly journals Seroprevalence and Real-time PCR Detection of Brucellosis in Abattoirs Animals as a Potential Route of Infection in Egypt

2019 ◽  
Vol 1 (5) ◽  
Author(s):  
Amira Mohamed Zakaria ◽  
Salwa F. Ahmed ◽  
Mohamed S. Motawae
Keyword(s):  
Real Time ◽  
Rose Bengal ◽  
Real Time Pcr ◽  
Serological Test ◽  
Pcr Amplification ◽  
Cost Effective ◽  
Rt Pcr ◽  
Serum Samples ◽  
Molecular Approaches ◽  
Rose Bengal Test

Brucellosis is among the most common and economically serious zoonosis worldwide. Brucellosis in Egypt is an endemic problem among animals and humans. This work intended to evaluate the conventional serological and molecular approaches as a tool for studying the prevalence of brucellosis within abattoir’s animals in two large Egyptian provinces.  Two hundred and thirty (n=230) blood and serum samples were collected  from (2-3) years male calves in two Egyptian abattoirs. Rose Bengal  test (RBT) and modified in-house ELISA were  applied  to determine the seroprevalence of  Brucellosis  in abattoirs animals while quantitative Taqman real-time PCRs (RT-PCR) were implemented for the characterization of Brucella species. The overall prevalence of brucellosis  in the two proveinces was (53.9 %) , (75.2 %) and (79.1 %) as determined by ELISA ,RBT and RT- PCR assays respectively. Brucella DNA was successfully amplified from serum samples as well as blood. A total of n= 182 samples (79.1 %) were identified by real time PCR amplification for IS711 gene as Brucella genus, n= 118 (64.8 %) were reported as B. aborts while n= 85 (46.7 %) were reported as B. melitensis. N= 44 (24.17 %) from the collected samples comprised the two species of bacteria. This study endorses the application of rose Bengal test as a sensitive and cost effective  serological test for brucellosis and real-time PCR as a distinguishing tool to detect the causative agents. Our findings indicate a significantly high prevalence of Brucella  antibodies and DNA in blood and serum samples  which poses a crucial  threat to public health in Egypt.  

scholarly journals Sero-epidemiological survey and risk factors associated with bovine brucellosis among slaughtered cattle in Nigeria

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Victor O. Akinseye ◽  
Hezekiah K. Adesokan ◽  
Akwoba J. Ogugua ◽  
Folashade J. Adedoyin ◽  
Patricia I. Otu ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Rose Bengal ◽  
Significant Risk ◽  
Epidemiological Survey ◽  
Bovine Brucellosis ◽  
Factors Associated ◽  
Rose Bengal Test ◽  
Geographical Regions ◽  
The Rose

Bovine brucellosis is endemic in Nigeria; however, limited data exist on nationwide studies and risk factors associated with the disease. Using a cross-sectional sero-epidemiological survey, we determined the prevalence of and risk factors for brucellosis in slaughtered cattle in three geographical regions of Nigeria. Serum samples from randomly selected unvaccinated cattle slaughtered over a period of 3 years (between December 2010 and September 2013) from northern, southern and south-western Nigeria were tested for antibodies to Brucella abortus using the Rose Bengal test. Data associated with risk factors of brucellosis were analysed by Stata Version 12. In all, 8105 cattle were screened. An overall seroprevalence of 3.9% (315/8105) was recorded by the Rose Bengal test, with 3.8%, 3.4% and 4.0% from the northern, southern and south-western regions, respectively. Bivariate analysis showed that cattle screened in northern Nigeria were less likely to be seropositive for antibodies to Brucella spp. than those from south-western Nigeria (odds ratio = 0.94; 95% confidence interval: 0.73–1.22). However, logistic regression analysis revealed that breed ( p = 0.04) and sex ( p £ 0.0001) of cattle were statistically significant for seropositivity to Brucella spp. The study found that brucellosis was endemic at a low prevalence among slaughtered cattle in Nigeria, with sex and breed of cattle being significant risk factors. Considering the public health implications of brucellosis, we advocate coordinated surveillance for the disease among diverse cattle populations in Nigeria, as is carried out in most developed countries.Keywords: Bovine brucellosis, RBT, Epidemiology, Public Health, Nigeria

scholarly journals Application of Rose Bengal Test and ELISA in Meat Juice for Monitoring of Brucellosis among Cattle Carcasses at Erbil City, Iraq

2019 ◽  
Vol 76 (1) ◽  
pp. 14
Author(s):  
Dhary Alewy AL MASHHADANY
Keyword(s):  
Rose Bengal ◽  
Standard Test ◽  
Health Concern ◽  
Economic Losses ◽  
Bacterial Disease ◽  
Bovine Brucellosis ◽  
Good Efficiency ◽  
Meat Juice ◽  
Rose Bengal Test ◽  
Meat Samples

Brucellosis is a global zoonotic bacterial disease. It is also an ever-increasing public health concern, particularly in endemic regions such as Kurdistan region in Middle East. Samples of meat juice were screened for anti-Brucella antibodies via Rose Bengal Test (RBT) and ELISA from January to June 2018. Bacteriological isolation of Brucella sp. from meat samples was also performed. The overall prevalence of bovine brucellosis is 7.7%, 6.6%, and 4.9% by RBT, ELISA, and culture-based test respectively. Based on sensitivity and specificity, ELISA outperforms RBT in comparison to culture results as the gold standard test. Nonetheless, both tests showed good efficiency in comparison to culture approach. In terms of temporal changes of brucellosis rate, spring progress is strongly associated (r²=0.96) with increase in seroprevalence. In conclusion, the prevalence of bovine brucellosis in Erbil city is alarming. Countermeasures should be taken to mitigate the economic losses and transmission to human.

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