In vitro screening of potato (Solanum tuberosum L.) cultivars for drought stress tolerance.

In supporting the replacement of potato varieties with the varieties that are adaptive to drought, effective and fast methods are needed. One of which is through in vitro screening of drought-tolerant genotypes. The aims of this research were to determine the appropriate concentration of sorbitol as a critical limit in the in vitro screening process of drought-tolerant genotypes, as well as to determine vegetative characteristics that could be used as indicators of in vitro selection and also to obtain in vitro drought-tolerant genotypes. To achieve these objectives, we used single-nodal explants of seven genotypes cultured for six weeks on MS media added with four levels of sorbitol concentration. Of the four treatments, sorbitol level that could distinguish susceptible and tolerant plants was 0.2 M sorbitol and at this concentration, the characteristics that showed significant interactions were plant height and root length, so that both characteristics could be used as selection indicators for the analysis of tolerance tests for the seven genotypes. The results of tolerance test using the analysis of relative decline and stress-susceptibility index (SSI) showed that PKHT4 and PKHT6 were the genotypes that were tolerant in vitro. Keywords: drought tolerant, in vitro, Solanum tuberosum, sorbitol

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In vitro screening of calli and evaluation their physiological states for the enhancement of regeneration efficiency in various potato (Solanum tuberosum L.) genotypes

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2020.101715 ◽

2020 ◽

Vol 28 ◽

pp. 101715

Author(s):

M. Harun-Or-Rashid ◽

S.M. Shahinul Islam ◽

M.A. Bari Miah ◽

Sreeramanan Subramaniam

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

In Vitro Screening ◽

Regeneration Efficiency

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Efficiency of pepper seed invigoration through hydrogen peroxide priming to improve in vitro salt and drought stress tolerance

Horticulture Environment and Biotechnology ◽

10.1007/s13580-020-00260-8 ◽

2020 ◽

Vol 61 (4) ◽

pp. 703-714

Author(s):

Najet Gammoudi ◽

Ines Karmous ◽

Khaled Zerria ◽

Mohamed Loumerem ◽

Ali Ferchichi ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Drought Stress ◽

Stress Tolerance ◽

Drought Stress Tolerance ◽

Salt And Drought Stress ◽

Pepper Seed

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Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

Journal of Agronomy and Crop Science ◽

10.1111/jac.12186 ◽

2016 ◽

Vol 203 (3) ◽

pp. 206-218 ◽

Cited By ~ 11

Author(s):

C. Bündig ◽

T. H. Vu ◽

P. Meise ◽

S. Seddig ◽

A. Schum ◽

...

Keyword(s):

Solanum Tuberosum ◽

Osmotic Stress ◽

Solanum Tuberosum L ◽

Drought Response ◽

In Vitro Screening ◽

Osmotic Stress Tolerance ◽

Pot Trials ◽

Starch Potato

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In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress

KnE Life Sciences ◽

10.18502/kls.v3i5.992 ◽

2017 ◽

Vol 3 (5) ◽

pp. 188

Author(s):

Bilter Anton Sirait ◽

Rosa Charloq

Keyword(s):

Tissue Culture ◽

Solanum Tuberosum ◽

Drought Stress ◽

Banding Pattern ◽

Solanum Tuberosum L ◽

In Vitro Study ◽

Dry Weight ◽

Leaf Chlorophyll ◽

Potato Plantlets

In vitro preliminary studies is candidate tolerant of potato (Solanum tuberosum L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to in vitro drought stress conditions using Polyethylene Glycol PEG. This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg · L–1, P2 = 6 000 mg · L–1. The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but the increase in the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09 in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.

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Sodium nitroprusside: its beneficial role in drought stress tolerance of “Mexican lime” (Citrus aurantifolia (Christ.) Swingle) under in vitro conditions

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-021-10218-9 ◽

2021 ◽

Author(s):

Marziyeh Jafari ◽

Ali Reza Shahsavar

Keyword(s):

Drought Stress ◽

Sodium Nitroprusside ◽

Stress Tolerance ◽

Citrus Aurantifolia ◽

Drought Stress Tolerance ◽

Mexican Lime ◽

Beneficial Role

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Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

10.25047/agropross.2017.15 ◽

2017 ◽

Author(s):

Yunita Prameswari ◽

FNU Djenal ◽

FNU Damanhuri

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

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