Serum anti-tissue transglutaminase IgA antibodies in patients with psoriasis vulgaris and relation to its severity

Introduction. Celiac disease (CD) is an autoimmune disease triggered by gluten in genetically predisposed individuals. Despite the increasing prevalence of CD, many patients remain undiagnosed. Standard serology tests are expensive and invasive, so several point-of-care tests (POC) for CD have been developed. We aimed to determine the prevalence of CD in first-grade pupils in Primorje-Gorski Kotar County, Croatia, using a POC test. Methods. A Biocard celiac test that detects IgA antibodies to tissue transglutaminase in whole blood was used to screen for celiac disease in healthy first-grade children born in 2011 and 2012 who consumed gluten without restrictions. Results. 1478 children were tested, and none of them were tested positive with a rapid test. In 10 children (0,6%), IgA deficiency has been suspected; only 4 of them agreed to be tested further for total IgA, anti-tTG, and anti-DGP antibodies. IgA deficiency was confirmed in 3 patients, and in all 4 children, CD has been excluded. Conclusion. Our results have not confirmed the usefulness of the POC test in screening the general population of first-grade schoolchildren. Further research is needed to establish the true epidemiology of CD in Primorje-Gorski Kotar County and to confirm the value of the rapid test in comparison with standard antibody CD testing.

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SEROLOGICAL SCREENING FOR CELIAC DISEASE IN CHILDREN WITH COLCHICINE-RESISTANT FAMILIAL MEDITERRANEAN FEVER

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.201800000-31 ◽

2018 ◽

Vol 55 (2) ◽

pp. 175-178

Author(s):

Yasin ŞAHIN ◽

Kenan BARUT ◽

Tufan KUTLU ◽

Fugen Cullu COKUGRAS ◽

Amra ADROVIC ◽

...

Keyword(s):

Celiac Disease ◽

Familial Mediterranean Fever ◽

Tissue Transglutaminase ◽

Intestinal Biopsy ◽

Control Group ◽

Serological Screening ◽

Iga Antibodies ◽

Mediterranean Fever ◽

Iga Antibody ◽

Clinical Conditions

ABSTRACT BACKGROUND: Familial Mediterranean fever and celiac disease share some common clinical features such as abdominal pain, diarrhea, arthralgia and arthritis. Also, both of the diseases are associated with many inflammatory and autoimmune diseases. Previous studies have shown the association between familial Mediterranean fever (FMF) and different clinical conditions. OBJECTIVE: We aimed to investigate the relationship between celiac disease and colchicine-resistant familial Mediterranean fever (crFMF) disease. METHODS: This prospective study was conducted at the Department of Pediatric Gastroenterology and Pediatric Rheumatology from October 2015 to August 2016. A total of 24 patients with crFMF were included in the study. We used 60 sex- and age-matched healthy subjects as a control group. Levels of total IgA and tissue transglutaminase (tTG) IgA antibody were measured in both groups. Those with increased level of tTG IgA were tested for anti-endomysium IgA antibodies (EMA). Gastroduodenoscopy and intestinal biopsy were planned for a definite diagnosis of celiac disease in patients with positive EMA. RESULTS: Of the 24 patients in this study, 18 (75.0%) were female. Only 4 (16.6%) of 24 patients were positive for tTG IgA. Patients with positive tTG IgA were then tested for EMA IgA antibodies and none of them had a positive result. Only one (1.6%) subject from the control group was positive for tTG IgA but EMA positivity was not detected. CONCLUSION: We did not found celiac disease in 24 children with crFMF. Since crFMF disease is rarely seen in general population, further studies with more patients are needed to provide more precise interpretation.

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A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2011.760 ◽

2012 ◽

Vol 50 (2) ◽

Cited By ~ 9

Author(s):

Karsten Conrad ◽

Dirk Roggenbuck ◽

Annelore Ittenson ◽

Dirk Reinhold ◽

Thomas Buettner ◽

...

Keyword(s):

Celiac Disease ◽

Tissue Transglutaminase ◽

Simultaneous Detection ◽

Serum Levels ◽

Iga Deficiency ◽

Enzyme Linked Immunosorbent Assay ◽

Iga Antibodies ◽

Work Up ◽

Simultaneous Assessment ◽

Dot Immunoassay

AbstractThis study investigated whether a dot immunoassay (DIA) can provide simultaneous detection of anti-tissue transglutaminase (tTG), anti-deamidated gliadin (DG) and total IgA antibodies, as required in the work-up of celiac disease (CD) patients.Celiac disease patients (n=111) consecutively diagnosed from 2001 to 2011 at the Children’s Hospital and Institute of Immunology (Technical University Dresden) were tested for anti-tTG, anti-DG and total IgA by enzyme-linked immunosorbent assay (ELISA) and DIA retrospectively. Blood donors (n=45) and non-CD individuals with low IgA serum levels (n=8) were included as controls. Antibodies to endomysial antigens (EmA) were assessed by indirect immunofluorescence (IIF).Four (3.6%) of 111 CD patients demonstrated an IgA deficiency with total IgA below 50 mg/L by ELISA. Total IgA of the 107 IgA-non-deficient CD patients varied from 70 to 6000 mg/L. All four IgA-deficient CD patients were detected by a reduced reaction control of DIA and demonstrated positive anti-tTG or anti-DG IgG by DIA or ELISA. Detection of anti-tTG and anti-DG by DIA and ELISA showed a very good agreement (IgA: κ=0.972, 0.856, respectively; IgG: 0.921, 0.895, respectively).Immunodot assay is a reliable and easy-to-use technique for the detection of IgA-deficient CD patients. Simultaneous assessment of anti-tTG and anti-DG IgA antibodies, and IgA deficiency by DIA can improve the efficacy of CD serology.

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Association between Levels of IgA Antibodies to Tissue Transglutaminase and Gliadin-Related Nonapeptides in Dermatitis Herpetiformis

The Scientific World JOURNAL ◽

10.1100/2012/363296 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Justyna Gornowicz-Porowska ◽

Monika Bowszyc-Dmochowska ◽

Agnieszka Seraszek-Jaros ◽

Elżbieta Kaczmarek ◽

Marian Dmochowski

Keyword(s):

Diagnostic Accuracy ◽

Receiver Operating Characteristic ◽

Causal Relationship ◽

Operating Characteristic ◽

Dermatitis Herpetiformis ◽

Tissue Transglutaminase ◽

Roc Curves ◽

Healthy Controls ◽

Iga Antibodies ◽

Healthy Persons

Dermatitis herpetiformis (DH) is an autoimmunity-driven inflammatory blistering dermatosis associated with a gluten-dependent enteropathy. Tissue transglutaminase (tTG) and nonapeptides of gliadin (npG) are considered in its pathomechanism/diagnostics. Here, the diagnostic accuracy of anti-tTG/anti-npG IgA ELISAs in Slavic DH patients with active skin rash was assessed through creating receiver operating characteristic (ROC) curves, determining cutoff values, and calculating correlations between levels of anti-tTG/anti-npG IgA in DH, IgA/neutrophil-mediated non-DH patients and healthy persons. Altogether, sera from 80 Slavic individuals were examined. There were negligible differences between cutoff points obtained by the ELISAs manufacturer and those in this study. There were statistically significant correlations between levels of anti-tTG/anti-npG IgA in both DH group and the group of IgA/neutrophil-mediated non-DH dermatoses. There was no such correlation in healthy controls. It seems that IgA autoantibodies to tTG and npG in the IgA/neutrophil-mediated DH are produced in the coordinated way implying their causal relationship.

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Hemolysis and IgA-antibodies against tissue transglutaminase: When are antibody test results no longer reliable?

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.22360 ◽

2017 ◽

Vol 32 (4) ◽

pp. e22360 ◽

Cited By ~ 3

Author(s):

Johannes Wolf ◽

Norman Haendel ◽

Johannes Remmler ◽

Carl Elias Kutzner ◽

Thorsten Kaiser ◽

...

Keyword(s):

Tissue Transglutaminase ◽

Antibody Test ◽

Test Results ◽

Iga Antibodies

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IgA Antibodies Against Gliadin and Tissue Transglutaminase in Dogs With Chronic Enteritis and Intestinal T-Cell Lymphoma

Veterinary Pathology ◽

10.1177/0300985817690212 ◽

2017 ◽

Vol 55 (1) ◽

pp. 98-107 ◽

Cited By ~ 5

Author(s):

I. Matsumoto ◽

K. Uchida ◽

K. Nakashima ◽

S. Hiyoshi ◽

J. K. Chambers ◽

...

Keyword(s):

T Cell ◽

Cell Lymphoma ◽

Tissue Transglutaminase ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Igg Antibodies ◽

Intestinal Lymphoma ◽

Serum Iga ◽

Iga Antibodies ◽

Clonality Analysis

Molecular clonality analysis of T-cell receptor (TCR) genes for diagnosing T-cell lymphoma is widely used in veterinary medicine. However, differentiating chronic enteritis (CE) from intestinal lymphoma is challenging because of the incompatibility between histopathologic and clonality analysis results. On the basis of findings that canine intestinal T-cell lymphoma and celiac disease share some common features, we conducted serologic examinations in combination with histopathologic and T-cell receptor clonality analyses in 48 dogs diagnosed with either CE or intestinal lymphoma. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies against gliadin and tissue transglutaminase (tTG) were quantitatively measured using ELISA. The conditions were classified according to the histopathologic diagnosis, clonality analysis, and combined histopathologic/clonality analysis. Histopathologic analysis showed that dogs with intestinal lymphoma were likely to have high levels of serum IgA antibodies against gliadin and tTG, and serum IgG antibodies against tTG. No correlation between the diagnosed groups and control group was observed in the results of the clonality analysis and histopathologic/clonality analysis. It is interesting that dogs with intestinal lymphoma had a higher serum IgA titer against gliadin and tTG than did dogs with CE. These results suggest an association between repetitive inflammatory stimulation by gliadin peptides and subsequent intestinal lymphoma in dogs.

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Celiac Disease After Administration of Immune Checkpoint Inhibitors: A Case Report

Frontiers in Immunology ◽

10.3389/fimmu.2021.799666 ◽

2021 ◽

Vol 12 ◽

Author(s):

Julie Leblanc ◽

Solene Hoibian ◽

Agathe Boucraut ◽

Jean-Philippe Ratone ◽

Louis Stoffaes ◽

...

Keyword(s):

Celiac Disease ◽

Immune Checkpoint ◽

Immune Checkpoint Inhibitors ◽

Gastrointestinal Endoscopy ◽

Checkpoint Inhibitors ◽

Villous Atrophy ◽

Tissue Transglutaminase ◽

Gluten Free ◽

Serum Iga ◽

Iga Antibodies

Immune checkpoint inhibitors (ICI) reinvigorate the immune system to recognize and destroy tumor cells. Because of this biological mechanism, patients might develop autoimmune toxicities, notably in the digestive tract (most frequently, hepatitis or colitis). A 70-year-old man with relapsed mesothelioma was treated with nivolumab in 3rd line. He was hospitalized for watery and foul-smelling diarrhea. He underwent gastrointestinal endoscopy, showing duodenitis and villous atrophy and measurement of serum IgA antibodies to tissue transglutaminase (tTG-IgA+), leading to the diagnosis of ICI-induced celiac disease. He was treated with steroids, proton pump inhibitors, and a gluten-free diet. If ICI-induced celiac disease is rare in the literature, increasing reports suggest that celiac disease might represent an underestimated ICI toxicity. This case highlights the necessity of complementary investigation (including tTG-IgA and endoscopic biopsies) in patients with atypical digestive symptoms during immunotherapy.

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Immunoassays for the detection of IgA antibodies to tissue transglutaminase: significance of multiples of the upper limit of normal and inter-assay correlations

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2015-0348 ◽

2016 ◽

Vol 54 (2) ◽

Cited By ~ 2

Author(s):

Brenda B. Suh-Lailam ◽

K. Wayne Davis ◽

Anne E. Tebo

Keyword(s):

Celiac Disease ◽

Indirect Immunofluorescence ◽

Tissue Transglutaminase ◽

Immunofluorescence Assay ◽

Indirect Immunofluorescence Assay ◽

Healthy Individuals ◽

Reference Test ◽

Upper Limit ◽

Iga Antibodies ◽

Diagnostic Pathways

AbstractThe presence of IgA antibodies to tissue transglutaminase (anti-tTg) is associated with variable risk for celiac disease. The use of common multiples of the upper limit of normal (ULN) has been suggested to optimize diagnostic pathways as well as improve harmonization between assays.The characteristics of four anti-tTG IgA assays relative to endomysial IgA (EMA) by indirect immunofluorescence assay (IFA) as reference test were assessed. Commutability between anti-tTG immunoassays and/or EMA based on manufacturer’s recommended cut-off values and three common multiples of ULN (3×, 5× and 10×) was also investigated. Sera from 200 patients and 100 healthy individuals were analyzed.At manufacturer’s cut-off; the sensitivities for the tTG assays ranged from 72.5% to 98.6% and specificities from 60.3% to 99.2%. The percent positive agreements between any anti-tTG and EMA or any two anti-tTG immunoassays varied from 56.7% to 98.0% and 46.7% to 100.0%, respectively. At 3×, 5× or 10× ULNs, the inter-rater reliability as measured by Cohen κ between any two anti-tTG assays were quite variable and ranged from 0.28 to 0.96, 0.26 to 0.89 or 0.13 to 0.78, respectively. Furthermore, the percent positive agreements between any two anti-tTg IgA immunoassays ranged from 83.1% to 98.2%, 92.0% to 100%, or 100%, at 3×, 5× or 10×, respectively.Commutability between tTG IgA immunoassays or tTG IgA and EMA is kit-dependent and common multiples of the ULN are not sufficient to correct for inter-assay variations. Many factors influence the performance of anti-tTG IgA assays which limit their commutability.

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