scholarly journals The Effect Follicular Fluid Vitamin A, E, D and B6 on Embryo Morphokinetics and Pregnancy Rates in Patients Receiving Assisted Reproduction

2019 ◽  
pp. 1
Author(s):  
Funda Gode ◽  
Suleyman Akarsu ◽  
Zeliha Gunnur Dikmen ◽  
Burcu Tamer ◽  
Ahmet Zeki Isik
Keyword(s):  
Vitamin A ◽  
Follicular Fluid ◽  
Significant Positive Correlation ◽  
Embryo Quality ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
In Vitro Fertilisation ◽  
Positive Correlation ◽  
Morphokinetic Parameters ◽  
Clinical Pregnancy Rates

<p><strong>Objective:</strong> To evaluate the associations among levels of vitamins A, E, D, and B6 in follicular fluid embryo morphokinetics and quality, and clinical pregnancy rates.</p><p><strong>Study Design:</strong> A total of 58 patients with unexplained infertility admitted to the in vitro fertilisation (IVF) centre of Izmir Medical Park Hospital were included in this prospective clinical study. For each patient, vitamin levels were assayed using high-performance liquid chromatography. After intracytoplasmic sperm injection, for each oocyte, the relationships between each vitamin and subsequent embryo quality, embryo morphokinetics, and clinical pregnancy rates were investigated. Embryos were classified as grade A, B, C, or D according to morphokinetic parameters using t5-t2 and t5-t3 (cc3). </p><p><strong>Results:</strong> There was no significant correlation between embryo morphokinetic parameters (tpnf, t2, t3, t4, t5, t6, t7 and t8) and follicular fluid vitamin (A, B6, D and E) levels (p&gt;0.05). There was a significant positive correlation between t5optimal and follicular fluid vitamin A levels (p&lt;0.05). There was a significant positive correlation between cc2optimal and follicular fluid vitamin B6 levels (p&lt;0.05). Levels of vitamins A and B6 were significantly higher in grade A and B embryos than in grade C and D embryos. There were no significant relationships between vitamins E or D and embryo quality or between any vitamin and clinical pregnancy rates.</p><p><strong>Conclusion:</strong> High levels of vitamins A and B6 in follicular fluid are significantly associated with high-quality embryos and optimal morphokinetics. However, none of the vitamins considered showed a significant relationship with clinical pregnancy rates.</p>

171 NORMAL VERSUS LOW ANTI-MULLERIAN HORMONE IN PATIENTS WITH ADVANCED MATERNAL AGE: IN VITRO FERTILIZATION OUTCOMES AND MORPHOKINETIC PARAMETERS

2016 ◽  
Vol 28 (2) ◽  
pp. 216
Author(s):  
A. Kotlyar ◽  
R. Flyckt ◽  
N. Desai
Keyword(s):  
Cell Cycle ◽  
Oocyte Retrieval ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Imaging Data ◽  
Vitro Fertilization ◽  
Ivf Outcomes ◽  
Morphokinetic Parameters ◽  
Clinical Pregnancy Rates

Anti-Mullerian hormone (AMH) levels are often used in an IVF population for assessment of ovarian reserve. However, it is not well understood whether AMH levels predict implantation and clinical pregnancy in older women undergoing IVF. In this study, we sought to compare IVF outcomes and morphokinetic parameters in patients 38 years and older with normal versus low AMH levels. Experimental design was a single-centre, retrospective cohort study. Time-lapse imaging data were evaluated for patients 38 years and older (n = 81). Patients were divided into those with AMH values less than 1.0 (n = 47) versus greater than or equal to 1.0 (n = 34). Morphokinetic data was then analysed from transferred blastocysts from these patients cultured in the Embryoscope until transfer (n = 100). The morphokinetic parameters examined were time to 2 cell (t2), 4 cell(t4), 5 cell (t5), time to synchronous divisions (s2, s3), duration of the second cell cycle (cc2), and third cell cycle (cc3). Implantation and clinical pregnancy were identified based upon the presence of a gestational sac. Fertilization, implantation, clinical pregnancy, and blastocyst formation rates were compared between normal and low AMH groups (Table 1). Mean morphokinetic parameters were compared between the 2 AMH groups using an unpaired t-test. Chi-squared tests were used for proportions. P < 0.05 was considered statistically significant. Results: Age of the patients ranged from 38–43, (41.3 ± 2.2 years). The results are summarised below. There were significant differences between implantation and clinical pregnancy rates for low and normal AMH levels. Morphokinetic parameters among the transferred embryos did not differ significantly between the 2 AMH groups (NS, data not shown). Conclusions: This initial data indicates that, in an IVF population 38 years and older, AMH levels greater than or equal to the cutoff of 1 ng mL–1 are associated not only with increased oocyte retrieval, but also with increased implantation rates and clinical pregnancy rates. These differences suggest that AMH may have a predictive role in determining the likelihood of clinical pregnancy in the 38 years and older patient population. Table 1.Oocyte harvest and IVF outcomes

The Non-linear Association Between Serum Level of Anti-M�llerian Hormone and in vitro Fertilisation Outcome

2019 ◽  
Vol 70 (9) ◽  
pp. 3392-3398
Author(s):  
Dragos Nicolae Albu ◽  
Alice Ioana Albu ◽  
Gabriel Octavian Olaru ◽  
Romina Marina Sima ◽  
Adrian Neacsu ◽  
...  
Keyword(s):  
Serum Level ◽  
Negative Impact ◽  
Fertilization Rate ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
In Vitro Fertilisation ◽  
Fertilisation Rate ◽  
Clinical Pregnancy Rates ◽  
Age Independent

Aim of the study was to analyse the relationship between Anti-M�llerian Hormone (AMH) serum level and in vitro fertilisation (IVF) with and without intra-cytoplasmic sperm injection (ICSI) outcome. We performed a retrospective study which included 1073 patients (mean age 34.68�4.28 years, mean body mass index 22.7�15.65 kg/m2) who performed IVF or IVF/ICSI between January 2013 and December 2016.We found that AMH serum level was age-independent positively related with oocytes (beta=0.329, p[0.0001) and zygotes number (beta=0.248, p[0.0001) and negatively correlated with fertilization rate (beta=-0.108, p=0.001). In multivariate regression, after adjustment for confounders, only oocytes number, but not AMH serum level, was associated with zygotes number (beta=0.814, p[0.0001) and fertilisation rate (beta=- 0.133, p=0.001). Patients with AMH in the range 1.1-5 ng/mL had significantly higher biochemical (65.3% versus 56.6%, p=0.009) and clinical pregnancy rates (57.7% versus 49.2%, p=0.014) in comparison with patients with AMH below 1.1 ng/mL and higher clinical pregnancy rates in comparison with patients with AMH above 7 ng/mL (57.7% versus 44%, p=0.011). Logistic regression analysis showed that AMH was positively associated with biochemical (OR 1.19, p=0.003) and clinical pregnancy (OR 1.16, p=0.009) independently of age and number of good embryos transferred in patients with AMH below 5 ng/mL. In turn, when only patients with normal ovarian reserve were analysed (AMH above 1.1 ng/mL), we found an age-independent negative association between AMH and clinical pregnancy (OR 0.93, p=0.014). AMH serum level is associated with both quantitative response (oocytes number) and qualitative parameters (pregnancy rate) during IVF/ICSI. We also found a bimodal relationship between AMH and pregnancy rates, which were positively associated in patients with AMH below 5 ng/mL, although higher AMH values seem to have a negative impact on pregnancy chances.

scholarly journals Precycle administration of GnRH antagonist and microdose HCG decreases clinical pregnancy rates without affecting embryo quality and blastulation

2006 ◽  
Vol 13 (4) ◽  
pp. 465-475 ◽  
Author(s):  
Orhan Bukulmez ◽  
Khurram S Rehman ◽  
Martin Langley ◽  
Bruce R Carr ◽  
Anna C Nackley ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Gnrh Antagonist ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rates
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