scholarly journals Prevalencia bayesiana de Staphylococcus aureus en vacas lecheras en el Valle del Cauca, Colombia

2021 ◽  
Vol 16 (3) ◽  
pp. 47-61
Author(s):  
Juan Carlos Gómez López ◽  
José Rafael Tovar Cuevas ◽  
Gustavo Bergonzoli ◽  
Armando Lucumí Moreno
Keyword(s):  
Staphylococcus Aureus ◽  
California Mastitis Test ◽  
Valle Del Cauca ◽  
National Mastitis Council

El Staphylococcus aureus es uno de los principales microorganismos causantes de mastitis subclínica en las vacas lecheras. Determinar la prevalencia del S. aureus se hace necesario en vacas de hatos lecheros grandes y pequeños ubicados en la región norte, centro y sur del departamento del Valle del Cauca, Colombia. Se utilizó la metodología del National Mastitis Council recolectando 1070 muestras de leche de 273 vacas en 17 hatos mediante un muestreo polietápico, durante el periódo 2015 - 2016. Se realizó cultivo con todas las muestras recolectadas independientemente del resultado de la prueba de California mastitis test. Se utilizó el análisis bayesiano de los datos para obtener estimaciones más precisas. La prevalencia bayesiana del patógeno S. aureus en vacas lecheras en el Valle del Cauca fue de 31,94% y se tiene un 95% de probabilidad de que el intervalo (30,03 – 37,62%) contenga el valor de dicha prevalencia. La región Norte, Centro y Sur evidenciaron prevalencias de 33%, 34% y 24% respectivamente. En cuanto al tamaño del hato se evidenció que las vacas de los hatos pequeños presentan mayor prevalencia con 47% que las vacas de los hatos grandes 17% a la presencia del patógeno. La alta prevalencia encontrada en las vacas genera un alto impacto en la salud pública debido a una posible contaminación zoonótica por este patógeno. 

scholarly journals THE OCCURRENCE OF BACTERIAL AGENTS CAUSING MASTITIS IN DAIRY SHEEP AND THEIR RESISTANCE TO ANTIBIOTICS

2020 ◽  
Vol 3 (7) ◽  
Author(s):  
František Zigo ◽  
Katarína Veszelits Lakticová ◽  
Mária Vargová M
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
High Resistance ◽  
Subclinical Mastitis ◽  
Causative Organism ◽  
Coagulase Negative Staphylococci ◽  
Isolation And Identification ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Acute Form

Introduction: The aim of this study was to monitor the occurrence of bacterial agents causing mastitis, forms of mastitis and antibiotic resistance in 300 ewes on a farm in Eastern Slovakia. Material and methods: During the milking season, were performed three complex investigations including clinical examination, California Mastitis Test and laboratory analysis of milk samples. The investigations and milk samples were taken in three phases; the beginning (April), the middle (June) and the end (September) of the milking season. Results: Of all the samples (806), 225 (28.0%) were positive for bacterial pathogens. The highest incidence of mastitis (33.3%) was recorded in September, while April (23.8%) and June (25.3%) had lower incidence. The samples from September showed the highest incidence of subclinical mastitis (20.1%), with 13.2% being an acute form of mastitis. Coagulase-negative staphylococci (CNS) were identified in 61.7% of the positive samples. Especially, S. chromogenes, S. epidermidis and S. schleiferi were most frequently isolated.  Staphylococcus aureus was the causative agent in 20.0% of the positive samples and caused acute or subclinical mastitis in the affected ewes. The tested bacteria showed very high resistance to novobiocin (59.5%) and penicillin (51.4%) and high resistance to amoxycillin (35.1%). We found that 80% of Staphylococcus aureus bacteria tested for antibiotic resistance were resistant to novobiocin and 70% were resistant to penicillin. Of all tested CNS, 56.5% were resistant to novobiocin, 39.1% to penicillin, and 34.7% to amoxicillin. Conclusion: Proper isolation and identification of the causative organism play a significant role in the prevention and control of the intramammary infection. In our study, a combinations of Streptomycin, Ciprofloxacin and Tetracycline were the most effective antibiotics for the control of mastitis.

scholarly journals Aspectos clínicos e laboratoriais da mastite induzida por inoculação intramamária de Staphylococcus aureus em vacas e búfalas

2014 ◽  
Vol 66 (5) ◽  
pp. 1299-1307
Author(s):  
A.M. Lazzari ◽  
M.V.S. Oliveira ◽  
B. Moreti ◽  
G. Guimarães ◽  
F. Krug ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
California Mastitis Test

Vários aspectos relacionados à mastite bubalina precisam ser esclarecidos. Muitas condutas são adaptadas a essa espécie pelo conhecimento adquirido com a espécie bovina. Com este experimento, objetivou-se evidenciar particularidades da mastite em búfalas e vacas que tiveram uma glândula inoculada com 1,0 x 103 UFC de S. aureus. Os animais foram monitorados por cultura bacteriana do leite, California Mastitis Test(CMT), e escores para avaliação da severidade da mastite. Foram utilizados os seguintes critérios: temperatura retal, apetite, produção de leite (resposta sistêmica à inflamação), contagem de células somáticas (CCS), aparência/consistência da glândula e aparência da secreção láctea (resposta localizada à inflamação). Todos os animais desenvolveram mastite clínica superaguda. A bactéria foi recuperada de todas as glândulas desafiadas, sem diferença significativa no percentual de isolamento entre as espécies até o 11º dia pós-inoculação, porém com diferença (P<0,001) no 30º dia pós-inoculação. A CCS pelo método eletrônico e o CMT detectaram nas búfalas resposta mais intensa logo após a inoculação e diminuição mais rápida da contagem ao final do experimento, quando se comparou essa espécie com as vacas. Ambos os testes demonstraram correlação com a cultura bacteriana. Na avaliação da severidade da mastite, as médias do escore total, do escore da resposta local e do escore da resposta sistêmica foram mais elevadas na espécie bovina (P<0,05). Ao longo do período de observação, verificou-se uma capacidade superior das búfalas em recuperar o status sanitário adequado, chegando, ao final do experimento, com os parâmetros avaliados mais próximos dos fisiológicos.

scholarly journals Preliminary study of homeopathic treatment of subclinical mastitis evaluated through somatic cells count (SCC) and California mastitis test (CMT)

2021 ◽  
Vol 7 (24) ◽  
pp. 147-151
Author(s):  
Zilda Cristiani Gazim ◽  
Orlando Seiko Takemura ◽  
Luiz Cláudio Monteiro da Silva ◽  
Carlos Massambani ◽  
Roberson Eduardo Moraes ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cells ◽  
Subclinical Mastitis ◽  
Clinical Aspects ◽  
Infection Level ◽  
Homeopathic Treatment ◽  
Holstein Breed ◽  
California Mastitis Test ◽  
End Of Treatment ◽  
Preliminary Study

The present paper presents the results of homeopathic treatment of 25 Holstein breed cows aged 3 to 8 years old diagnosed with subclinical mastitis through California Mastitis Test (CMT). Animals were divided into 3 groups according with infection level. A homeopathic complex was developed on the grounds of clinical aspects, including Phosphorus 30x, Phytolacca 30x, Silicea 30x, Sulphur 30x, Belladona 30x, Bryonia alba 30x, Pulsatilla 30x, Calendula 30x and biotherapic of Staphylococcus aureus 200x. The remedy was added to salt and was administered to cattle 100g/cow/day for 75 days. CMT were carried out every 2 weeks to control incidence and severity of mastitis; somatic cells count (SCC) was performed at the beginning and the end of treatment. CMT showed significant improvement in regression of infection level all throughout the study; final SCC showed decrease in 82% of animals, signaling thus efficacy of the homeopathic treatment. Keywords:  Subclinical mastitis; Dairy cows; Homeopathy; California Mastitis Test, Somatic Cells Count.   Estudo preliminar do tratamento homeopático da mastite subclínica avaliado por contagem de células somáticas (SCC) e California Mastitis Test (CMT) Resumo Este artigo apresenta os resultados do tratamento homeopático de 25 vacas raça Holstein entre 3 e 8 anos de idade diagnosticadas com mastite subclínica através do California Mastitis Test (CMT). Os animais foram divididos em 3 grupos de acordo com o nível da infecção. Foi preparado um complexo homeopático com base nos achados clínicos, composto de: Phosphorus 30X, Phytolacca 30x, Sulphur 30x, Belladona 30x, Bryonia alba 30x, Silicea 30x, Pulsatilla 30x, Calendula 30x s 30X, Phytolacca 30x, Sulphur 30x, Belladona 30x, Bryonia alba 30x, Silicea 30x, Pulsatilla 30x, Calendula 30x e bioterápico de Staphylococcus aureus 200x. O complexo foi acrescentado no sal e administrado na dose de 100g/vaca/dia. O CMT foi realizado a cada 2 semanas a fim de monitorar a incidéncia e gravidade da mastitie; a contagem de células somáticas (SCC) foi realizada ao início e no final do tratamento. Os valores do CMT mostraram melhora significativa no sentido de regressão do nível da infecção ao longo do estudo; o valor final da SCC diminuiu em 82% dos animais, apontando para a eficácia do tratamento homeopático. Palavras-chave:  Mastite subclínica; Vacas leiteiras; Homeopatia; CMT, contagem de células somáticas.   Estudio preliminar del tratamiento homeopático de la mastitis sublínica evaluado mediante recuento de células somáticas (SCC) y California Mastitis Test (CMT) Resumen Este artículo presenta los resultados del tratamiento homeopático de 25 vacas raza Holstein entre 3 e 8 años de edad diagnosticadas com mastitis sucblínica mediante California Mastitis Test (CMT). Los animales fueron divididos en 3 grupos según el nível de la infección. Fue compuesto un complejo homeopático con base en la presentación clínica, incluyendo Phosphorus 30X, Phytolacca 30x, Sulphur 30x, Belladona 30x, Bryonia alba 30x, Silicea 30x, Pulsatilla 30x, Calendula 30x y bioterápico de Staphylococcus aureus 200x. El complejo fue agregado en sal y administrado a los animales en la dosis de 100g/vaca/día. CMT fue realizado cada 2 semanas para monitorizar la incidencia y gravedad de la mastitis; recuento de células somáticas (SCC) fue realizado al inicio y al final del tratamiento. CMT mostró mejoría significativa en el sentido de regresión del nivel de la infección a lo largo del estudio; el valor final del SCC disminuyó en 82% de los animales, apuntando para la eficacia del tratamiento homeopático. Palabras-clave:  Mastitis subclínica; Vacas lecheras; Homeopatía; CMT, recuento de células somáticas.   Correspondence author:  Zilda Cristiani Gazim, [email protected] How to cite this article:  Barzon CD, Medeiros F, Moraes RE, Silva LCM, Massambani C, Takemura OS, Gazim ZC. Preliminary study of homeopathic treatment of subclinical mastitis evaluated through somatic cells count and California mastitis test. Int J High Dilution Res [online]. 2008 [cited YYYY Mmm DD]; 7(24): 147-151. Available from: http://journal.giri-society.org/index.php/ijhdr/article/view/292/364.  

Dairy cow characteristics related to Staphylococcus aureus isolation from quarter samples

1995 ◽  
Vol 62 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Carsten Enevoldsen ◽  
Yrjö T. Gröhn ◽  
Iver Thysen
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Herds ◽  
Test Reaction ◽  
Cross Sectional ◽  
Staph Aureus ◽  
California Mastitis Test ◽  
Microbiological Examination ◽  
Potential Risk Factors ◽  
Sole Ulcers ◽  
Clinical Measures

SummaryA comprehensive description of the health status of 2406 cows in 28 herds was used to study complex interrelationships between Staphylococcus aureus occurrence and potential risk factors at the cow level over a broad spectrum of production conditions. The objective of the study was to characterize those cows shedding Staph, aureus in milk and to provide indications of possible causal relations that should be further explored. The estimates are expected to aid in providing valid and precise evaluations of results from cross sectional microbiological examination of bovine (quarter) milk samples. Staph, aureus were isolated at microbiological examination of quarter milk from 5·2, 13·5, 17·9 and 22·0% of 1552, 490, 296 and 277 cows in lactations 1, 2, 3 and 4–9 respectively. Simultaneous isolation of other mastitis pathogens was not significantly associated with occurrence of Staph, aureus. Of the positive samples, 95% had a positive California mastitis test reaction. Among the clinical measures of udder health, only visibly abnormal milk combined with normal udder tissue was associated with increased occurrence of Staph, aureus, although not consistently. Prior antibiotic treatment for udder disorders was not significantly associated with Staph, aureus isolation. The occurrence of sole ulcers in multiple digits was consistently associated with Staph, aureus in the first lactation. Milk yield was involved in interactions with other pathogens isolated, prior veterinary disease treatment, body weight and season of calving. Strong herd-year effects were revealed. This, combined with the interactions, indicated that some herd-specific factors were major determinants of Staph, aureus occurrence. The study indicated which cow characteristics should be taken into account in epidemiological analyses and causal interpretations of data from cross sectional microbiological examinations of dairy herds.

Coagulase gene polymorphism and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine subclinical mastitis milk in Sidi-Bel-Abbes, Algeria

2017 ◽  
Vol 7 (1) ◽  
pp. 21-27
Author(s):  
Ahmed Reda Belmamoun ◽  
Abdelkader Ammam ◽  
Imene Berrabah ◽  
Karima Bereksi Reguig
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Pcr Amplification ◽  
Screen Test ◽  
Raw Milk ◽  
Subclinical Mastitis ◽  
Latex Agglutination ◽  
Pcr Analysis ◽  
California Mastitis Test ◽  
Coa Gene

The study was conducted to identify and characterize Staphylococcus aureus in raw milk derived from subclinical mastitis in Sidi-Bel-Abbes Algeria. In this paper, we explore the possibility of detection of the coagulase gene (coa), which encodes the coagulase enzyme, by PCR analysis in antibiotic-resistant isolates, with the latex agglutination phenotype and free coagulase.Out of 336 samples of raw milk examined with California mastitis test (CMT) posi-tive; a total of 142 samples were bacteriologically positive with 56.34% Staphylococcus isolates, 21 (26.25%) isolates were confirmed as S.aureus. Nineteen (90.48%) isolates of S.aureus showed free coagulase on the tube agglutination test. Two atypical S.aureus strains (9.52%) were defective for the clumping factor and / or protein A , determined with the Staphytect plus test and the tube coagulase test. The isolates of S.aureus were resistant to penicillin and tetracycline with 76.19%. Two isolates (9.52%) of S.aureus re-sistant to meticillin (MRSA) were detected in this study, with a MIC of ≥4 μg / liter and a cefoxitin screen test with a MIC of ≥8 μg / liter, and 13 (61.9%) isolates were with a multiresistance phenotype. The 21 isolates were sub-jected to PCR amplification of the 3' end of the coa gene, 18 (85.71%) were revealed on a 1% agarose gel with a single band between 547 bp and 875 bp. The use of the PCR genotypic test to identify the profile of the coa gene can be used as an appropriate identification criterion for differentiating coagulases from S.aureus and for understanding their epidemiology.

scholarly journals Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from Milk in Shire Dairy Farms, Tigray, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Weldemelak Girmay ◽  
Getachew Gugsa ◽  
Habtamu Taddele ◽  
Yisehak Tsegaye ◽  
Nesibu Awol ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Bovine Mastitis ◽  
Pcr Amplification ◽  
Dairy Farms ◽  
Cross Sectional Study ◽  
Penicillin G ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
California Mastitis Test

Antibiotic-resistant Staphylococcus aureus isolates pose a severe challenge to both veterinary and health professions and dairy cattle producers. Cross-sectional study was conducted from November 2014 to May 2015 to isolate and identify S. aureus from mastitic cows’ milk and estimate the occurrence of MRSA in the dairy farms of Shire. Physical examination and California mastitis test were performed on a total of 220 dairy cows. Bacteriological isolation and identification and antibiogram testing were performed. Furthermore, multiplex polymerase chain reaction (PCR) was done for the detection of mec A and fem A genes. Out of the 220 dairy cows, 64 (29.09%) were positive for bovine mastitis, and of these, 32.81% were coagulase-positive S. aureus (CoPS). Antibiogram test results showed 100% of the isolates were resistant to penicillin G, nalidixic acid, and ampicillin, and 33.33% of the CoPS showed resistance to oxacillin (phenotypically MRSA positive). But 38.09% of the CoPS were found to be resistant and susceptible to vancomycin. PCR amplification of the seven phenotypically MRSA isolates revealed that 42.9% and 71.4% of them were found to carry fem A and mec A genes, respectively. The current study revealed the existence of alarming level of CoPS and development of multidrug resistance.

scholarly journals Impact of Subclinical Mastitis-causing Bacterial Species on the Composition and Chemical Properties of Milk

2021 ◽  
Author(s):  
Tarik Safak ◽  
Ali Risvanli ◽  
Zulal Ascı-Toraman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Bacterial Species ◽  
Chemical Properties ◽  
Subclinical Mastitis ◽  
Milk Composition ◽  
Mineral Matter ◽  
California Mastitis Test ◽  
And Staphylococcus Aureus

Background: Here we investigate whether the composition and some chemical properties of milk can correlate with bacterial species causing subclinical mastitis in cows. Methods: One hundred and eighty cows were used in the study. The California Mastitis Test (CMT) was applied to the selected cows. The cows were divided into four groups: cows with negative CMT (n = 45), the Escherichia coli (n = 45), Streptococcus agalactiae (n = 45) and Staphylococcus aureus (n = 45) groups. Result: Milk composition and some chemical properties were measured. The highest fat ratio (5.82±0.34%) was found in the Escherichia coli group (p less than 0.05). The proportions of solid-non-fat (9.67±0.09%), protein (3.51±0.03%), lactose (5.29±05%) and mineral matter (0.75±0.01%) were lowest in the Escherichia coli group (p less than 0.05). The electrical conductivity of milk was lowest in the CMT-negative group (4.23±0.02 mS/cm), while the Streptococcus agalactiae group had the highest value (4.61±0.02 mS/cm) (p less than 0.05). The specific gravity of the Escherichia coli group was lower than the CMT-negative (1,034.91±0.56 kg/m3) and Staphylococcus aureus (1,034.24±0.65 kg/m3) groups (p less than 0.05). Based on our findings, we propose that milk composition and some chemical parameters are altered in subclinical mastitis. However, these could not be standardized according to the responsible bacterial species. We recommend that these parameters are monitored regularly on dairy farms.

Sign in / Sign up

Export Citation Format

Share Document