Study of the low temperature anoxic ammonia oxidation feasibility

2015 ◽  
Vol 10 (1) ◽  
pp. 172-177 ◽  
Author(s):  
Yu. A. Nikolaev ◽  
M. N. Kozlov ◽  
M. V. Kevbrina ◽  
A. G. Dorofeev ◽  
V. G. Aseeva ◽  
...  
Keyword(s):  
Low Temperature ◽  
Ammonia Oxidation ◽  
Nitrifying Bacteria ◽  
Anammox Bacteria ◽  
Nitrification Rate ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Digested Sludge ◽  
Anammox Process ◽  
Heterotrophic Denitrification

The paper presents results of study of anoxic ammonia oxidation at low temperature conducted at JSC Mosvodokanal, Moscow, Russia. The study was carried out in two reactors, 5 l each, operated at the temperature of 5–10 °С. Both reactors were inoculated with the soil, collected from waste water sludge landfill, that presumably, contained low-temperature adapted nitrifying bacteria. Reactor No. 1 contained nitrifying bacteria only. Reactor No. 2 was further inoculated by anammox bacteria. Filtrate from digested sludge belt thickeners was added into the reactors to achieve a final N-NH4 concentration of 70–95 mg/l. The reactors were operated as sequencing batch reactors. After 90 days of incubation maximal nitrification rate in reactor No. 1 was 1.4 mg N-NH4/g VSS*h, and in reactor No. 2–1.0 mg N-NH4/g VSS*h. Estimated doubling time of nitrifying bacteria was 45 days. Total mineral nitrogen removal in the 1st reactor was 20% (via process of heterotrophic denitrification), and in the 2nd – 60% (via both the processes of heterotrophic and autotrophic nitrate reduction). Through the process of autotrophic denitrification (anammox), two times as much nitrogen was removed, compared to the heterotrophic denitrification process. Anammox process rate was 0.4 mg N-NH4/g VSS*h.

scholarly journals Nitrogen removal via a single-stage PN–Anammox process in a novel combined biofilm reactor

2017 ◽  
Vol 77 (6) ◽  
pp. 1483-1492 ◽  
Author(s):  
Yue-mei Han ◽  
Feng-xia Liu ◽  
Xiao-fei Xu ◽  
Zhuo Yan ◽  
Zhi-jun Liu
Keyword(s):  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Ammonia Oxidation ◽  
Biofilm Reactor ◽  
Nitrifying Bacteria ◽  
Anammox Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
High Ammonia ◽  
Total Nitrogen Removal ◽  
Anammox Process

Abstract This study developed a partial nitrification (PN) and anaerobic ammonia oxidation (Anammox) process for treating high-ammonia wastewater using an innovative biofilm system in which ammonia oxidizing bacteria grew on fluidized Kaldnes (K1) carriers and Anammox bacteria grew on fixed acryl resin carriers. The airlift loop biofilm reactor (ALBR) was stably operated for more than 4 months under the following conditions: 35 ± 2 °C, pH 7.5–8.0 and dissolved oxygen (DO) of 0.5–3.5 mg/L. The results showed that the total nitrogen removal efficiency reached a maximum of 75% and the total nitrogen removal loading rate was above 0.4 kg/(d·m3). DO was the most efficient control parameter in the mixed biofilm system, and values below 1.5 mg/L were observed in the riser zone for the PN reaction, while values below 0.8 mg/L were observed in the downer zone for the Anammox reaction. Scanning electron microscopy and Fluorescence In Situ Hybridization images showed that most of the nitrifying bacteria were distributed on the K1 carriers and most of the Anammox bacteria were distributed within the acryl resin carriers. Therefore, the results indicate that the proposed combined biofilm system is easy to operate and efficient for the treatment of high-ammonia wastewater.

Biological Nitrogen Removal under Low Temperature in a Membrane Separation Bioreactor

1993 ◽  
Vol 28 (10) ◽  
pp. 325-333 ◽  
Author(s):  
C. Chiemchaisri ◽  
K. Yamamoto
Keyword(s):  
Low Temperature ◽  
Nitrogen Removal ◽  
Oxygen Transfer ◽  
Membrane Separation ◽  
Nitrifying Bacteria ◽  
Biological Nitrogen Removal ◽  
Nitrification Rate ◽  
Temperature Decrease ◽  
Operational Cycle ◽  
Biological Nitrogen

Biological nitrogen removal under low temperature in a membrane separation bioreactor for on-site domestic wastewater treatment was studied. The bioreactor was operated under intermittent aeration of a 180-minute operational cycle to achieve simultaneous nitrification and denitrification for nitrogen removal. During stepwise temperature decrease from 25°C to 5°C at every two weeks duration, nitrogen removal started to deteriorate as temperature dropped to 10°C. It decreased from more than 90% at 25°C to 20% at 5°C as a result of inhibition of nitrification at low temperature. However, increasing oxygen supply, i.e. increasing aeration time in operational cycle, could completely recover nitrification at 10°C. Nitrogen removal could be achieved by introducing non-aeration period after complete nitrification was obtained. Average nitrogen removal was 90 and 85% under 10 and 5°C respectively. The results indicated that sufficient oxygen transfer could be maintained in the membrane separation bioreactor even if the temperature was as low as 5°C. Analysis of respiratory quinone component of sludge suggested the decrease of strict aerobic bacteria percentage in mixed liquor during temperature decrease and increase of their percentage during the recovery of nitrification at 10°C. These changes could be related to the nitrification through the changes of oxygen transfer condition in the system. Insignificant difference of maximum volumetric nitrification rate obtained at 25 and 5°C probably suggests that there was not much difference in oxygen availability for nitrifying bacteria between both the temperatures once high and stable nitrogen removal was achieved.

Efficient and integrated start-up strategy for partial nitrification to nitrite treating low C/N domestic wastewater

2009 ◽  
Vol 60 (12) ◽  
pp. 3243-3251 ◽  
Author(s):  
Jianhua Guo ◽  
Shuying Wang ◽  
Huijun Huang ◽  
Yongzhen Peng ◽  
Shijian Ge ◽  
...  
Keyword(s):  
Domestic Wastewater ◽  
Kinetic Mechanism ◽  
Partial Nitrification ◽  
Nitrifying Bacteria ◽  
Nitrite Accumulation ◽  
Fish Analysis ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Start Up ◽  
Operation Results

Nitrogen removal via the nitrite pathway has the potential of reducing the requirements for aeration consumption and carbon source. However, the development of an efficient and quick start-up strategy for partial nitrification to nitrite has proven difficult in the treatment of low strength wastewater. In this study, the feasibility of partial nitrification achieved by using real-time aeration duration control was not only demonstrated from the kinetic mechanism, but also was validated in three sequencing batch reactors (SBRs) fed with low C/N domestic wastewater. Nitrite accumulation could be achieved when aeration was terminated as soon as an inflexion pH point was reached (the dpH/dt became from negative to positive). The reduction or limitation of the NOB growth could be achieved through aeration duration control, due to leaving no extra time for NOB to convert the accumulated nitrite. The experimental operation results also showed that partial nitrification with nitrite accumulation ratios of over 80% was achieved successfully in these three reactors with process control. Fluorescence in situ hybridization (FISH) analysis indicated the reduction of NOB was achieved and AOB became the dominant nitrifying bacteria. Moreover, an integrated start-up strategy based on aeration duration control was proposed to quickly achieve partial nitrification to nitrite.

Low temperature nitrogen removal in sequencing batch reactors

1988 ◽  
Vol 22 (9) ◽  
pp. 1163-1171 ◽  
Author(s):  
J.A. Oleszkiewicz ◽  
S.A. Berquist
Keyword(s):  
Low Temperature ◽  
Nitrogen Removal ◽  
Batch Reactors ◽  
Sequencing Batch Reactors

scholarly journals Promotion of partial nitritation-anammox process by improving granule proportion

2017 ◽  
Vol 75 (11) ◽  
pp. 2580-2585 ◽  
Author(s):  
Jun Cheng ◽  
Liang Zhang ◽  
Yandong Yang ◽  
Shujun Zhang ◽  
Xiaoyu Han ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
System Performance ◽  
Mass Fraction ◽  
Removal Rate ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Ammonium Oxidation ◽  
Partial Nitritation ◽  
Granule Fraction ◽  
Anammox Process

For enhancing the partial nitritation-anammox (PN/A) process, the effects of granule fraction on system performance were investigated in this study. Two sequencing batch reactors (SBRs) were inoculated with PN/A biomass with a floc mass fraction of 53%. In SBR1, when the nitrogen removal rate (NRR) was stable, flocculent sludge was gradually discharged from the reactor using a screen, and the granule fraction was therefore increased. However, nitrogen removal was not improved and finally deteriorated due to the loss of nitritation activity. In SBR2, most flocculent sludge was eliminated and granular proportion was maintained at over 90% by controlling a short settling and decanting time. NRR was low initially but gradually improved to 1.23 kg N/(m3·d), which was 54% higher than SBR1. Ammonium oxidation activities of flocs and granules were respectively measured. Results suggested that the increase of nitritation activity in the granules was the main reason for the improvement of nitrogen removal in SBR2.

scholarly journals Pertumbuhan Bakteri Anaerobic Ammonia Oxidation (Anammox) Pada Salinitas 2 dan 9 Persen

2018 ◽  
Vol 9 (2) ◽  
pp. 55-62
Author(s):  
Wijanarka Wijanarka ◽  
Sudarno Sudarno ◽  
Novi A. Pratama
Keyword(s):  
Waste Water ◽  
Environmental Effect ◽  
Ammonia Oxidation ◽  
Ammonium Nitrogen ◽  
Anammox Bacteria ◽  
Nitrogen Gas ◽  
Selective Media ◽  
Gram Staining ◽  
Anaerobic Ammonia Oxidation ◽  
Anammox Process

When ammonia in waste water is lost inappropriately, it  will raise an adverse environmental effect for the aquatic cycle. Anammox, anaerobic ammonia oxidation, is a novel process in which nitrite is used as an electron acceptor in the conversion of ammonium to nitrogen gas. The anammox process removes ammonium in the autrotrophic system by leaving little biomass. This study aims to analyze the effect of salinity on the growth of anammox bacteria. The samples used were from the brackish water sediments of the East Flood Canal River of Semarang. The isolation was done by gram staining and the bacteria were inoculated on media with different salinity concentration and the growth was measured using spectrophotometer. The results showed that anammox bacteria had a higher growth rate of 3% (control) when it was grown on a medium with a concentration of 9%. Anammox bacteria grown on anammox selective media showed that the bacteria were able to adapt to environments with different salinity concentrations of 2% and 9%. Key words: anammox, ammonium, nitrogen, anammox bacteria.

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