Packed-Entrapped-Mixed Microbial Cells for Small Wastewater Treatment

1990 ◽  
Vol 22 (3-4) ◽  
pp. 343-350 ◽  
Author(s):  
P. Y. Yang ◽  
M. L. Wang

The primary goal of the research is to develop and evaluate a biological treatment system in which mixed microbial populations are physically immobilized by entrapment. The immobilized system was evaluated initially for its ability to remove simple benzene-based compounds from a synthetic wastewater as a model for treating pesticide-containing waters. Cellulose triacetate was used as the preparation of monocarrier and combined cellulose triacetate and calcium alginate were used for the preparation of bi-carrier. Phenol was used as substrate to test the entrapped mixed microbial cell process. Process performance including steady state, shock load and comparison of entrapped microbial cell process with and without entrapped microbial cell was investigated. It was concluded that the critical loading rate for phenol synthetic wastewater appeared to be 9 g COD/L/day. The COD removal efficiency could be maintained higher than 90%. Because of the low effluent suspended solid concentration, conventional-secondary-settling tanks could be eliminated. Also, the process could take both concentration and hydraulic shock loads of phenol synthetic wastewater successfully without the need of external sludge recycling. The equalization and external sludge recycling facilities equipped in the conventional wastewater treatment plant could be eliminated. When the process is operated only at low COD loading rate (less than 1.3 g/L/day), entrapped cells might not be required. However, when operated at higher loading rate, combined adsorbed and entrapped cells appeared to remove phenol more efficiently. For the application, mono-carrier (cellulose triacetate) is preferable to bi-carrier (cellulose triacetate and calcium alginate), since it is easier to prepare and the operational performance is better. Therefore, the application of packed-entrapped mixed microbial cell process for treatment of small-toxic-organic wastewater holds great potential.

1994 ◽  
Vol 29 (10-11) ◽  
pp. 487-495 ◽  
Author(s):  
P. Y. Yang ◽  
T. Ma ◽  
T. S. See ◽  
N. Nitisoravut

The entrapment of mixed microbial cell was accomplished by using a porous material, such as cellulose triacetate, to confine the migration of microorganism. Synthetic glucose, phenol, carbaryl and nitrate wastewater was used as substrate to evaluate this process. Applying entrapped microbial cell techniques for biological water/wastewater treatment was previously investigated mainly by using the pure culture system. For the past six years, the University of Hawaii has investigated this technology for biological wastewater treatment by using a mixed culture approach. It was found that the developed carrier, using cellulose triacetate, is the most suitable for providing the necessary mechanical strength and durability under both the aerobic and anaerobic conditions. It also provides many advantages, such as a short start-up period, high effluent quality in terms of COD, NO3-N concentrations and removal efficiency, low effluent suspended solid concentration, and high SRT with low HRT. The process has potential to replace or upgrade the existing biological processes for water and wastewater treatment. A pre-engineered and packaged plant can be easily developed for a small wastewater treatment system for toxic or hazardous wastewater.


2004 ◽  
Vol 49 (5-6) ◽  
pp. 281-288 ◽  
Author(s):  
S.J. Kim ◽  
P.Y. Yang

A two-stage entrapped mixed microbial cell (2SEMMC) process which separates nitrification and denitrification phases by the installation of the anoxic and oxic EMMC reactors packed with EMMC carriers was operated with 6, 4, 3, and 2 hours of hydraulic retention time (HRT) using simulated domestic wastewater. The activated sludge was immobilized using cellulose acetate for the EMMC carriers. Similar soluble chemical oxygen demand (SCOD) removal efficiencies of 90-97% were observed for all HRTs (SCOD loading rate of 0.84-2.30 g/L/d) applied. In order to achieve more than 80 % of TN removal efficiency, the HRT should be maintained higher than 4 hours (less than 0.24 g/L/d of TN loading rate). Denitrification was a rate-limiting step which controlled overall TN removal efficiency at TN loading rate of 0.15-0.31 g/L/d although nitrification efficiencies achieved 97-99 %. The effluent TSS of less than 25 mg/L in the 2SEMMC process was maintained at the SCOD loading rate of less than 1.23 g/L/d with back-washing intervals of 5 and 10 days in the anoxic and oxic EMMC reactors, respectively. The minimum HRT of 4 hours is required for high removal efficiencies of organics (average 95.6 %) and nitrogen (average 80.5 %) in the 2SEMMC process with 3 times of recirculation ratio.


2013 ◽  
Vol 2 (2) ◽  
pp. 131-135
Author(s):  
Z Yavari ◽  
H Izanloo ◽  
K Naddafi ◽  
H.R Tashauoei ◽  
M Khazaei

Renewable energy will have an important role as a resource of energy in the future. Microbial fuel cell (MFC) is a promising method to obtain electricity from organic matter andwastewater treatment simultaneously. In a pilot study, use of microbial fuel cell for wastewater treatment and electricity generation investigated. The bacteria of ruminant used as inoculums. Synthetic wastewater used at different organic loading rate. Hydraulic retention time was aneffective factor in removal of soluble COD and more than 49% removed. Optimized HRT to achieve the maximum removal efficiency and sustainable operation could be regarded 1.5 and 2.5 hours. Columbic efficiency (CE) affected by organic loading rate (OLR) and by increasing OLR, CE reduced from 71% to 8%. Maximum voltage was 700mV. Since the microbial fuel cell reactor considered as an anaerobic process, it may be an appropriate alternative for wastewater treatment


2011 ◽  
Vol 396-398 ◽  
pp. 1989-1994
Author(s):  
Kun Hu ◽  
Zhen Zhong Li ◽  
Yong Guo ◽  
Wei Xing Huang

Systematic experiments were carried out with synthetic wastewater in order to investigate the operating conditions and kinetics of wastewater treatment in a three-phase biological fluidized bed reactor. It is found that with the COD loading rate changed stepwise from 3.5 to 11.2 kg COD/m3•d, the reactor can rapidly restore from each impact of COD load and maintain the removal rate for COD at 85% or above, presenting a high flexibility for the variation of organic loading rate (OLR) in the influent. While the operating OLR keeps constant, the increase of influent COD and NH3-N concentrations will be followed by the correspondingly increased removal rates, so that the COD and NH3-N concentrations in the effluent keep nearly constant. The batched experiments under different air supply conditions show that there exists an optimal air flow rate at which the most effective treatment can be achieved. Finally, a kinetics model of the continuous treatment process was derived based on mass balance and Monod equations, and the kinetic constants were determined by the experimental data at steady operating OLR.


1990 ◽  
Vol 22 (7-8) ◽  
pp. 153-160 ◽  
Author(s):  
Pradeep Kumar ◽  
R. J. Garde

With increasing stress on existing wastewater treatment systems, it is necessary either to upgrade the treatment unit(s) or install an entirely new treatment plant. Obviously, the upgrading is preferred over the alternative of having a new system. Keeping this in view, in the present project, an attempt has been made to explore the possibility of upgrading existing facultative ponds using water hyacinth. Bench-scale batch studies were designed to compare the performance of hyacinth treatment system with facultative ponds. Investigations were carried out with synthetic wastewater having COD in the range of 32.5-1090 mg/l. The efficiency of COD removal in water hyacinth ponds was 15-20 percent more than the facultative ponds. Based on the results, an empirical model has been proposed for COD removal kinetics. In the second phase of the project a hyacinth pond was continuously operated. BOD, COD, TS, TN, TP, pH, and DO were regularly monitored. However, the DO of the effluent from hyacinth treatment system was considerably reduced. Effluent should be aerated before it is discharged. The results indicate that the existing facultative ponds can be stalked with water hyacinth to improve their performance as well as hyacinth treatment systems can be installed to support the conventional treatment.


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