Removal of fecal indicator organisms and parasites (fecal coliforms and helminth eggs) from municipal biologic sludge by anaerobic mesophilic and thermophilic digestion

2001 ◽  
Vol 44 (4) ◽  
pp. 97-101 ◽  
Author(s):  
M. Rojas Oropeza ◽  
N. Cabirol ◽  
S. Ortega ◽  
L. P. Castro Ortiz ◽  
A. Noyola
Keyword(s):  
Treatment Plant ◽  
Anaerobic Treatment ◽  
Fecal Coliforms ◽  
Anaerobic Sludge ◽  
Indicator Organisms ◽  
Fecal Indicator ◽  
Class A ◽  
Helminth Eggs ◽  
Start Up ◽  
Thermophilic Digestion

In this work, two egg-shaped, 5L-volume, anaerobic sludge digesters were used, one under mesophilic conditions (35°C, M1), and the other under thermophilic conditions (55°C, T1). Both digesters were fed with the purged sludge from an anaerobic treatment plant (start-up period) and from an activated sludge plant (stabilization period), treating municipal wastewaters. The purpose of the study was to establish the technical feasibility of the anaerobic thermophilic sludge treatment comparatively, during the stages of start-up and stabilization of the process, for removing pathogenic microorganisms and parasites efficiently. The results show that, in both stages, the anaerobic thermophilic digester presents higher efficiency on the removal of pathogens and parasites, than the mesophilic digester. Anaerobic thermophilic digestion is close to complying with the EPA (1996) limits for “Class A” type biosolids, referring to the number of parasitic helminth eggs (0.25 HELarval/gTS), and to the pathogen indicator fecal coliforms (<1000 MPN/gTS). Therefore, the results show that thermophilic anaerobic digestion of biologic sludge may be considered as a suitable technology for the production of Class A biosolids, for further use in agriculture without restrictions.

Single-stage Sequencing Batch Process for Producing Class A Biosolids from AD Effluent at Terminal Island Treatment Plant

2006 ◽  
Vol 1 (4) ◽  
Author(s):  
Huub H.J. Cox ◽  
Steve Fan ◽  
Reza Iranpour
Keyword(s):  
Fecal Coliform ◽  
Treatment Plant ◽  
Batch Process ◽  
Single Stage ◽  
Fecal Coliforms ◽  
Class A ◽  
Truck Loading ◽  
Train Operation ◽  
Plant Control ◽  
Class A Biosolids

Terminal Island Treatment Plant converted its digesters to thermophilic operation with the objective to comply with the U.S. EPA Part 503 Biosolids Rule requirements for Class A biosolids. The following processes were tested: a) single-stage continuous; b) two-stage continuous; c) single-stage sequencing batch. Salmonella sp. were always non-detect in digester outflows (<3 MPN/4 g dry wt), whereas fecal coliform densities were usually below the Class A limit of 1000 MPN/g dry wt. However, the recurrence of fecal coliforms in post-digestion caused non-compliance with the Class A limit at the truck loading facility as the last point of plant control for compliance. After several design modifications of the post-digestion train, operation of the digesters as sequencing batch digesters according to the time-temperature requirement of Alternative 1 of the Part 503 Biosolids Rule achieved compliance for both Salmonella sp. and fecal coliforms at the last point of plant control (truck loading facility).

Anaerobic Treatment of Polyethylene Terephthalate (PET) Wastewater from Lab to Full Scale

1999 ◽  
Vol 40 (8) ◽  
pp. 229-236 ◽  
Author(s):  
F. Fdz-Polanco ◽  
M. D. Hidalgo ◽  
M. Fdz-Polanco ◽  
P. A. García Encina
Keyword(s):  
Polyethylene Terephthalate ◽  
Loading Rate ◽  
Treatment Plant ◽  
Textile Wastewater ◽  
Anaerobic Treatment ◽  
Cod Removal ◽  
Organic Loading Rate ◽  
Foam Formation ◽  
Organic Loading ◽  
Start Up

In the last decade Polyethylene Terephthalate (PET) production is growing. The wastewater of the “Catalana de Polimers” factory in Barcelona (Spain) has two main streams of similar flow rate, esterification (COD=30,000 mg/l) and textile (COD=4000 mg/l). In order to assess the anaerobic treatment viability, discontinuous and continuous experiments were carried out. Discontinuous biodegradability tests indicated that anaerobic biodegradability was 90 and 75% for esterification and textile wastewater. The textile stream revealed some tendency to foam formation and inhibitory effects. Nutrients, micronutrients and alkali limitations and dosage were determined. A continuous lab-scale UASB reactor was able to treat a mixture of 50% (v) esterification/textile wastewater with stable behaviour at organic loading rate larger than 12 g COD/l.d (0.3 g COD/g VSS.d) with COD removal efficiency greater than 90%. The start-up period was very short and the recuperation after overloading accidents was quite fast, in spite of the wash-out of solids. From the laboratory information an industrial treatment plant was designed and built, during the start-up period COD removal efficiencies larger than 90% and organic loading rate of 0.6 kg COD/kg VSS.d (5 kg COD/m3.d) have been reached.

The relationship between three potential pathogens and pollution indicator organisms in Nova Scotian coastal waters

1983 ◽  
Vol 29 (10) ◽  
pp. 1261-1269 ◽  
Author(s):  
W. J. Robertson ◽  
R. S. Tobin
Keyword(s):  
Membrane Filtration ◽  
Indicator Bacteria ◽  
Fecal Coliforms ◽  
Most Probable Number ◽  
Indicator Organisms ◽  
Fecal Indicator ◽  
Filtration Method ◽  
Probable Number ◽  
Pollution Indicator ◽  
Nova Scotian

Fifteen stations, in two estuaries, along the Northumberland Strait of Nova Scotia were examined between June and September 1981 for a relationship between the concentrations of commonly monitored fecal indicator bacteria and the potential pathogens Candida albicans, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Increased densities of these three organisms were usually associated with high densities of indicator bacteria. Whereas C. albicans and P. aeruginosa occur in human fecal wastes, V. parahaemolyticus is indigenous to the marine environment and positively responds to elevated nutrient levels in sewage. There is also some evidence that these bacteria survive as long or longer in marine waters than the common indicator bacteria. While membrane-filtration techniques for the enumeration of C. albicans and P. aeruginosa proved satisfactory, a V. parahaemolyticus membrane-filtration method lacked specificity and was supplemented by a most-probable-number method. In marine recreational and shellfish waters, these three organisms could complement fecal coliforms and fecal streptococci as indicators of human fecal contamination.

scholarly journals Quality Assessment of Full-Scale Municipal Wastewater Treatment Plant Consisting UASB Reactors and Polishing Ponds During its Start-Up Phase in India

2016 ◽  
Vol 11 (1) ◽  
pp. 47-55
Author(s):  
Nadeem Khalil ◽  
Tarique Ahmad
Keyword(s):  
Waste Water ◽  
Wastewater Treatment ◽  
Quality Assessment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Municipal Sewage ◽  
Anaerobic Sludge ◽  
General Hypothesis ◽  
Start Up ◽  
Uasb Reactors

Amongst the technologies available, the up flow anaerobic sludge blanket (UASB) process has been one of the most widely applied methods for municipal waste water treatment especially in countries of warm climatic conditions like India. However, past about one decade has witnessed rapid decline in the UASB popularity and its implementation. There has been criticism from various sections on the performance of UASB reactors for not complying with the prescribed discharge standards. It is a general hypothesis that the UASB reactors are not meant for diluted waste water like municipal sewage when typically the BOD is less than 150 mg/l, COD 250 mg/l and sulphates are more than 150mg/l. An attempt has been made through this study to investigate the reasons on the basis of quality assessment and field observations on UASB reactors and it’s post-treatment of a newly commissioned (start-up) municipal (sewage) wastewater treatment plant commonly called ‘STP’ having capacity of 14 million litres per day (MLD). Study was aimed to know the gaps during the commissioning stage which could be related to poor removal efficiencies. This paper briefly discusses some issues related to operation and maintenance of the UASB plants with purpose for improvements.

New Technologies for Anaerobic Wastewater Treatment

1986 ◽  
Vol 18 (12) ◽  
pp. 41-53 ◽  
Author(s):  
Look Hulshoff Pol ◽  
Gatze Lettinga
Keyword(s):  
Wastewater Treatment ◽  
New Technologies ◽  
Anaerobic Treatment ◽  
Full Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Anaerobic Wastewater Treatment ◽  
Start Up ◽  
Uasb Reactors ◽  
Sludge Granulation

Presently anaerobic wastewater treatment is becoming an accepted simple technology for the treatment of a variety of wastewaters. Of the different treatment systems that have been developed the UASB process (Upflow Anaerobic Sludge Blanket) has found the widest application. Almost all of the more than 60 full scale UASB reactors in operation now, are running satisfactorily. The excellent sludge retention generally found in UASB-reactors is obtained by sludge granulation, which can be seen as a sludge immobilization process. The presently available insight into the sludge granulation process is briefly presented, together with the strategy to be applied for performing a proper first start-up and secondary start-up of UASB reactors, viz. using granular seed sludge. The effect of the presence of SS with regard to the loading potentials of anaerobic treatment systems will be discussed. The experiences obtained with some full scale applications of the UASB-process are presented.

scholarly journals Comparing Source of Agricultural Contact Water and the Presence of Fecal Indicator Organisms on the Surface of ‘Juliet’ Grape Tomatoes

2013 ◽  
Vol 76 (6) ◽  
pp. 967-974 ◽  
Author(s):  
DONNA M. PAHL ◽  
ADRIANA TELIAS ◽  
MICHAEL NEWELL ◽  
ANDREA R. OTTESEN ◽  
CHRISTOPHER S. WALSH
Keyword(s):  
Water Quality ◽  
Tomato Fruit ◽  
Quality Standards ◽  
Water Sources ◽  
Fecal Coliforms ◽  
Indicator Organisms ◽  
Water Quality Standards ◽  
Fecal Indicator ◽  
Microbial Water Quality ◽  
Fruit Surface

Consumption of fresh tomatoes (Solanum lycopersicum) has been implicated as the cause of several foodborne illness outbreaks in the United States, most notably in cases of salmonellosis. How the levels of fecal indicator organisms (FIOs) in water relate to the counts of these microorganisms on the tomato fruit surface is unknown, although microbial water quality standards exist for agricultural use. This study utilized four types of FIOs currently and historically used in microbial water quality standards (Enterobacteriaceae, total coliforms, fecal coliforms, and Escherichia coli) to monitor the water quality of two surface ponds and a groundwater source. The groundwater tested contained significantly lower counts of all FIOs than the two surface water sources (P < 0.05). Considerable variability in bacterial counts was found in the surface water sources over the course of the season, perhaps explained by environmental variables, such as water temperature, pH, precipitation, and air temperature (R2 of 0.13 to 0.27). We also monitored the fruit surface of grape tomatoes treated with overhead applications of the different water sources over the 2009 and 2010 growing seasons. The type of water source and time of year significantly affected the populations of FIOs in irrigation water (P < 0.05). Despite up to 5-log differences in fecal coliforms and 3-log differences in E. coli between the water sources, there was little difference in the populations measured in washes taken from tomato fruits. This lack of association between the aforementioned FIOs present in the water samples and on the tomato fruit surface demonstrates the difficulty in developing reliable metrics needed for testing of agricultural water to ensure the effectiveness of food safety programs.

Start-Up Strategy to Achieve Excellent Efficiency of Degradation Acetate,Ethanol and Propionate in UASB

2011 ◽  
Vol 71-78 ◽  
pp. 2103-2106
Author(s):  
Ming Yue Zheng ◽  
Ming Xia Zheng ◽  
Kai Jun Wang ◽  
Hai Yan
Keyword(s):  
Loading Rate ◽  
Anaerobic Treatment ◽  
Upflow Anaerobic Sludge Blanket ◽  
Organic Loading Rate ◽  
Anaerobic Sludge ◽  
Organic Loading ◽  
Metabolic Intermediate ◽  
Loading Rates ◽  
Start Up ◽  
Anaerobic Sludge Blanket

The performance of upflow anaerobic sludge blanket (UASB) fed with three metabolic intermediate (acetate, ethanol, and propionate) respectively was studied. The degradation of metabolic intermediate were investigated to discuss the reason for propionate inhibition problem in anaerobic treatment. The hydraulic retention time (HRT) in the reactors started with 8.0h.The yield rate of biogas were 237ml/gCOD, 242ml/gCOD, 218ml/gCOD for acetate, ethanol and propionate, respectively when finishing start-up under OLR of 5.0 kgCOD/(m3·d) (HRT=9.6h).The HRT remained constant 9.6h,and the substrate concentration was gradually increased from 1,000 to 16,000mg/L as COD,and the organic loading rates(OLR) was from 3.0 to 40.0 kgCOD/(m3·d).The maximum propionate concentration was 41.6 gHPr-COD/L at the organic loading rate of 43.9 kgCOD/(m3·d) (HRT, 9.6h) as well as acetate and ethanol.

Removal of helminth eggs and fecal coliforms by anaerobic thermophilic sludge digestion

2002 ◽  
Vol 45 (10) ◽  
pp. 269-274 ◽  
Author(s):  
N. Cabirol ◽  
M. Rojas Oropeza ◽  
A. Noyola
Keyword(s):  
Primary Treatment ◽  
Fecal Coliforms ◽  
Volatile Fraction ◽  
Waste Sludge ◽  
Biological Sludge ◽  
Sludge Stabilization ◽  
Sludge Digestion ◽  
Helminth Eggs ◽  
Thermophilic Conditions ◽  
Thermophilic Digestion

Anaerobic digestion of two types of waste sludge was applied in order to assess the suitability of thermophilic conditions for the stabilization of organic matter and removal of fecal coliforms and helminth eggs. Feeding sludge was taken from an activated sludge municipal facility (BS) and from an enhanced primary treatment municipal plant (EPT). As an accompanying experiment, mesophilic digesters were also operated. The four digesters (M1, M2, T1, T2) had a 5 litre volume and an egg shape. A highly stabilized material was obtained at both temperatures with BS type of sludge, taking the reduction of volatile fraction of suspended solids (%RVSS) as indicator (84% for M1 and 74% for T1). In general, EPT sludge was a more difficult substrate, if compared with BS sludge; thermophilic condition was better adapted than mesophilic for this kind of sludge. Satisfactory reductions on counts of fecal coliforms and helminth eggs were achieved under thermophilic digestion for both types of feeding sludge. T1 digester, fed with biological sludge, removed fecal coliforms below 1000 MPN/gTS and helminth eggs down to 0.28 HELarval/gTS, at an HRT of 20 days. As a general conclusion, anaerobic thermophilic digestion may be an appropriate option for sludge stabilization, in order to meet EPA Class A biosolids final disposal regulations. However, further research is needed in order to consistently remove helminth eggs and fecal coliforms from waste sludge at shorter hydraulic retention times.

Reactivation and/or growth of fecal coliform bacteria during centrifugal dewatering of anaerobically digested biosolids

2004 ◽  
Vol 50 (9) ◽  
pp. 115-120 ◽  
Author(s):  
Y.N. Qi ◽  
S. Gillow ◽  
D.S. Herson ◽  
S.K. Dentel
Keyword(s):  
Fecal Coliform ◽  
Treatment Plant ◽  
Land Application ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
Human Pathogens ◽  
Indicator Organisms ◽  
Treatment Facility ◽  
Fecal Coliform Bacteria ◽  
Media Type

Fecal coliform bacteria are used as indicator organisms for the presence of pathogens. In sludges, it has often been assumed that the counts of fecal coliforms after digestion (where the sludges may also be called biosolids) are representative of the counts when the sludge is disposed or recycled, such as by land application. The possibility has been raised, however, that dewatering processes can lead to increased counts of fecal coliforms and, by inference, human pathogens. This paper presents data from previous studies of this possibility; the results were inconsistent but showed observable increases in fecal coliforms at one treatment plant. Additional studies were then performed at another treatment facility, which showed statistically significant increases in fecal coliform counts after dewatering and two days of aging. The increases exceeded two orders of magnitude and included two centrifuge types and two biosolids types. Artifacts of media type and enumeration method have been excluded, and shearing of the material by commercial blender did not produce the same effects.

Anaerobic treatment of Wastewater from a fish-canning factory in a full-scale Upflow Anaerobic Sludge Blanket (UASB) reactor

1999 ◽  
Vol 40 (8) ◽  
pp. 57-62 ◽  
Author(s):  
A. Pun˜al ◽  
J. M. Lema
Keyword(s):  
Anaerobic Treatment ◽  
Upflow Anaerobic Sludge Blanket ◽  
Total Alkalinity ◽  
Uasb Reactor ◽  
Organic Loading Rate ◽  
Anaerobic Sludge ◽  
Stable Operation ◽  
Start Up ◽  
Upward Velocity ◽  
Anaerobic Sludge Blanket

The start-up and optimisation of a 380 m3 UASB reactor (Up-flow Anaerobic Sludge Blanket) treating wastewater from a fish-canning factory was carried out. At the beginning of the operation the Organic Loading Rate (OLR) was 1 kg COD/m3·d. Then, the load was gradually increased in steps of 50% OLR until the final capacity of the system (4 kg COD/m3·d) was achieved. Wastewater characteristics were highly dependent on the canned product (mussel, tuna, sardines, etc.). In spite of that, a stable operation working at a hydraulic retention time (HRT) of 2 days was maintained. Total Alkalinity (TA) always presented values higher than 3 g CaCO3/l, while the IA/TA ratio (Intermediate Alalinity/Total Alkalinity) was always maintained lower than 0.3. In order to improve granulation conditions, upward velocities from 0.5 to 0.8 m/h were applied. The highest values caused the washout of non-granulated biomass from the reactor, optimum operation being achieved at an upward velocity of 0.7 m/h.

Sign in / Sign up

Export Citation Format

Share Document