Denitrifying activity measurements using an anoxic titration (pHstat) bioassay

2002 ◽  
Vol 46 (9) ◽  
pp. 211-218 ◽  
Author(s):  
K.M. Foxon ◽  
C.J. Brouckaert ◽  
A. Rozzi

An anoxic titrimetric test was investigated for measuring denitrifying activity in an activated sludge system. The method measures the amount of acid that is required to maintain the pH set-point value in a batch denitrification experiment. An iterative algorithm was implemented to extract nitrate uptake rate (NUR) data from titration data, since the accumulation and depletion (stripping) of reaction by-products HCO3- and CO2 affects the direct calculation of denitrifying activity from titration data. This method was performed using an automatic pH-stat acid dosing system, and the data were analysed using the simulation software package, AQUASIM.

2021 ◽  
Vol 36 (4) ◽  
pp. 373-378
Author(s):  
Haewon Jung ◽  
Dal-Jae Yun ◽  
Hoon Kang

An image focusing method for holographic subsurface radar (HSR) is proposed herein. HSR is increasingly being utilized to survey objects buried at shallow depths and the acquired signals are converted into an image by a reconstruction algorithm. However, that algorithm requires actual depth and material information or depends on human decisions. In this paper, an entropy-based image focusing technique is proposed and validated by numerical simulation software package based on finite-difference time-domain method and experiment. The resulting images show good agreement with the actual positions and shapes of the targets.


2015 ◽  
Vol 11 (5) ◽  
pp. 10 ◽  
Author(s):  
Paulo Abreu ◽  
Manuel Romano Barbosa ◽  
António Mendes Lopes

This paper presents the use of a virtual lab for teaching industrial robots programming to university students. The virtual lab, that replicates the existing physical lab, is built using an industrial simulation software package, RobotStudio™. The capabilities of this tool are explored in order to complement the introduction of theoretical concepts with practical programming experience. In addition to illustrate the use of different coordinate systems in a robotic cell, a description of the tool center point calibration and examples of evaluating different moving strategies to cover a plane surface, are also presented.


1989 ◽  
Vol 35 (8) ◽  
pp. 1688-1693 ◽  
Author(s):  
N W Tietz ◽  
J R Astles ◽  
D F Shuey

Abstract Using recent knowledge regarding the roles of colipase, bile acids, Ca2+, and emulsifiers, we optimized a previously published pH-Stat method for lipase (EC 3.1.1.3) activity measurements. The recommended assay conditions are: olive oil/triolein, 100 mL/L; sodium glycocholate, 35 mmol/L; Ca2+, 8.5 mmol/L; and colipase, 6.0 mg/L. The sample volume is 0.10 mL, the reaction pH 9.0, the temperature 30 degrees C, and the concentration of titrant 15 mmol/L. Hydroxypropyl methylcellulose, 20 g/L, replaces acacia as emulsifier to avoid inhibition by excess Ca2+. The standard curve is linear to greater than 4566 U/L. The reference interval with olive oil as substrate is 30-235 U/L. Lipase activities with triolein substrate are 9.9% greater than with olive oil. Interference by pancreatic carboxylesterase (EC 3.1.1.1) activity is inhibited by incubating the sample with diisopropylfluorophosphate. Results correlate well with those by the optimized SingleVial method of Boehringer Mannheim Diagnostics (r = 0.997) and the immunochemical assay of Beckman Instruments, Inc. (r = 0.995). Correlation with the aca method (E.I. DuPont de Nemours & Company) is less satisfactory (r = 0.892), probably owing to lack of colipase in the latter method.


2020 ◽  
Vol 180 ◽  
pp. 04005
Author(s):  
Vergil Muraru ◽  
Constantin Calinoiu ◽  
Sebastian Muraru ◽  
Ana Dulgheru ◽  
Cornelia Muraru-Ionel

The paper presents the results of research on the performance of linear electro-hydraulic servomechanisms with stepper motor, evaluated by mathematical modelling and numerical simulation with the AMESIM software package. These servomechanisms have a great potential for application, including for agricultural technical equipment. Servomechanisms of various types are used for the rapid and high precision operation of the various systems. In practice, electro-hydraulic servomechanisms have been required in the case of the systems requiring high forces or moments. These equipment are automatic hydraulic tracking systems and can be analysed with methods specific to automatic systems. The paper contains a structural description of an electro-mechanical servomechanism with stepper motor, its operation, mathematical model and its performances obtained by numerical simulation. The mathematical modelling of the electro-hydraulic servomechanism is based on the relations between the input sizes and output sizes of the equipment in its structure as well as the connection relations between these equipment. Based on the obtained results it is demonstrated that the analysis and synthesis of electro-hydraulic servomechanisms with stepper motor can be determined by numerical simulation using the AMESIM simulation software.


2010 ◽  
Vol 26-28 ◽  
pp. 900-904 ◽  
Author(s):  
Yong He ◽  
Guo Fu Ding ◽  
Yi Sheng Zou ◽  
Mei Wei Jia ◽  
Ming Heng Xu

An object-oriented modeling method was proposed to develop a simulation software package named GVDS which could be used to predict some aspects of dynamic behavior of railway vehicle. The package based on multi-body dynamics mainly consists of three parts, an interactive pre-processor, the solver and an interactive post-processor. With UML, demands and structure of the software package are represented. By modeling of the geometry and behavior of each object, virtual prototype of railway vehicle is formed and by the simulation, the critical speed of hunting stability, wheel-rail contact forces and so on can be determined and the hunting stability, curving behavior and ride comfort can be analyzed and evaluated. Finally, some cases are simulated. The simulation results show the effectiveness of the proposed method.


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