Evaluation of estrogen-like activity on sewage treatment processes using recombinant yeast

2002 ◽  
Vol 46 (11-12) ◽  
pp. 367-373 ◽  
Author(s):  
K. Onda ◽  
S.-Y. Yang ◽  
A. Miya ◽  
T. Tanaka

Four sewage treatment plants based on an activated sludge process and a pilot scale plant for advanced sewage treatment located in Japan were evaluated for removal of estrogenic substances using in vitro recombinant yeast assay and chemical analysis. The results indicated that 17βl-estradiol (E2) significantly contributed to estrogen-like activity analyzed by yeast assay especially in secondary treated effluents. On the other hand, batch study showed that estrogen-like activity of spiked E2 was easily decreased by an activated sludge treatment. This result suggested that E2 concentrations measured by enzyme immunoassay (EIA) were interpreted as false positives in effluents, and that unknown estrogenic substances other than E2 might have contributed to estrogen-like activity in the secondary treated effluents. Further, in the pilot scale study, advanced sewage treatment processes such as a biological aerated filtration (BAF) process, an advanced oxidation process (AOP), were effective for the removal of those estrogenic activities contributed by unknown estrogenic substances in sewage secondary treated effluent.

2005 ◽  
Vol 51 (10) ◽  
pp. 221-229 ◽  
Author(s):  
P.D. Beavers ◽  
I.K. Tully

Small communities that are sewered by either package sewage treatment plants or on-site sewerage facilities are finding that the ground and surface waters are being contaminated. Nitrogen, which typically is not removed in these conventional systems, is a major concern. This project evaluated the capability of four sewage treatment technologies to reduce the amount of nitrogen being discharged in the effluent to the receiving environment. The four sewage treatment processes evaluated include a recirculating sand filter, biofilter, slow sand filter and constructed subsurface flow wetland. These processes were evaluated for their capability to reduce nitrogen, phosphorus, BOD5 and TSS. The primary objective of the project was to evaluate the capability of these treatment processes to reduce nitrogen using biological processes nitrification and denitrification. This paper reports on the performance of these processes to reduce nitrogen. The study demonstrated that the biofilter was capable of removing from a primary treated influent 40% of the total nitrogen. For the same quality influent the recirculating sand filter was capable of removing 35% of the total nitrogen. Secondary treated effluent was fed to the slow sand filter and the subsurface flow wetland. There was a 52% reduction in total nitrogen through the wetland however there was virtually no reduction in total nitrogen through the slow sand filter.


2009 ◽  
Vol 59 (10) ◽  
pp. 2029-2036 ◽  
Author(s):  
A. Arelli ◽  
L. Luccarini ◽  
P. Madoni

Digital image analysis is a useful tool to estimate some morphological parameters of flocs and filamentous species in activated sludge wastewater treatment processes. In this work we found the correlation between some morphological parameters and sludge volume index (SVI). The sludge was taken from a pilot—scale activated sludge plant, owned by ENEA, located side stream to the Trebbo di Reno (Bologna, Italy) municipal WWTP and fed by domestic wastewater. In order to use image analysis, we developed a correct method to acquire digital microbiological observations and to obtain images altogether representative of the sludge properties. We identified and assessed the parameters needed to estimate the settleability of the sludge and evaluated the morphological filamentous features. It is known that several conditions (i.e. low F/M, nutrient deficiency, low dissolved oxygen) select specific filamentous species and their excessive growth decrease floc-forming/filaments ratio, correspond to the worse settleability properties; we found a relationship between the relative abundance of filamentous species and SVI. We also evaluated the fractal dimension parameter (FD) and determined a threshold value useful to distinguish between the “weak” and “firm” floc and we found a correlation between FD and SVI.


2020 ◽  
Vol 12 (11) ◽  
pp. 4758
Author(s):  
Huyen T.T. Dang ◽  
Cuong V. Dinh ◽  
Khai M. Nguyen ◽  
Nga T.H. Tran ◽  
Thuy T. Pham ◽  
...  

Fixed-film biofilm reactors are considered one of the most effective wastewater treatment processes, however, the cost of their plastic bio-carriers makes them less attractive for application in developing countries. This study evaluated loofah sponges, an eco-friendly renewable agricultural product, as bio-carriers in a pilot-scale integrated fixed-film activated sludge (IFAS) system for the treatment of municipal wastewater. Tests showed that pristine loofah sponges disintegrated within two weeks resulting in a decrease in the treatment efficiencies. Accordingly, loofah sponges were modified by coating them with CaCO3 and polymer. IFAS pilot tests using the modified loofah sponges achieved 83% organic removal and 71% total nitrogen removal and met Vietnam’s wastewater effluent discharge standards. The system achieved considerably high levels of nitrification and it was not limited by the loading rate or dissolved oxygen levels. Cell concentrations in the carriers were twenty to forty times higher than those within the aeration tank. Through 16S-rRNA sequencing, the major micro-organism types identified were Kluyvera cryocrescens, Exiguobacterium indicum, Bacillus tropicus, Aeromonas hydrophila, Enterobacter cloacae, and Pseudomonas turukhanskensis. This study demonstrated that although modified loofah sponges are effective renewable bio-carriers for municipal wastewater treatment, longer-term testing is recommended.


Chemosphere ◽  
2008 ◽  
Vol 71 (8) ◽  
pp. 1582-1592 ◽  
Author(s):  
Jiho Lee ◽  
Byoung C. Lee ◽  
Jin S. Ra ◽  
Jaeweon Cho ◽  
In S. Kim ◽  
...  

2003 ◽  
Vol 48 (2) ◽  
pp. 349-356 ◽  
Author(s):  
J.M. Broome ◽  
P.M. Morris ◽  
J.G. Nanthambwe

The most economic combination of unit treatment processes for a new sewage treatment works in Zimbabwe was found to be anaerobic ponds followed by trickling filters. The regulations governing irrigation with treated effluent permitted the omission of humus tanks or further treatment. Two stage anaerobic ponds are desludged by gravity through fixed sludge outlet pipework. Sludge is disposed of by irrigation of a Eucalyptus plantation. Novel features of the inlet works and pond outlets are also described. The works has functioned for eight years without major problems, but the assumption that humus tanks or settling ponds were not required may have been mistaken. The sludge removal system has worked well. Without the sludge pipework, it is estimated that desludging of the primary ponds would have been required after two years of operation, but they have now operated successfully for eight years. The combination of anaerobic ponds and trickling filters should be considered where land availability or site conditions make facultative ponds difficult or expensive to construct.


2021 ◽  
Vol 11 (2) ◽  
pp. 15-24
Author(s):  
Nur Diana Wakimin ◽  
Juferi Idris ◽  
Lydia Dundun Francis ◽  
Maureen Neging ◽  
Siti Rafiqah Muskil ◽  
...  

Pollutants from sewage wastewater are major concerns due to their environmental effects. Thus, an effective sewage wastewater treatment plant is important to ensure discharged effluent is well treated before it can be released to rivers or water streams. This study aims to evaluate the performance of the Activated Sludge Hi-Kleen Treatment Plant (ASHTP) located at H Block (ASHTP at H Block) and L Block (ASHTP at L Block) in UiTM Sarawak Branch, Samarahan Campus, Sarawak, Malaysia. The evaluation was based on physicochemical and removal efficiency namely pH, temperature, turbidity, chemical oxygen demand (COD), and total suspended solids (TSS) from raw influent and treated effluent. The findings indicated that the overall performance of both ASHTP at H Block and L Block were satisfactory where treated effluent meets the standard discharge limits of Environmental Quality (Sewage) Regulations 2009 (Standard B). For ASHTP at H Block, the removal efficiencies of turbidity, COD, and TSS were found to be 86.00, 13.76, and 88.02% respectively, in which the pH, temperature, turbidity, COD, and TSS of the treated effluent were 7.30, 28.10°C, 10.40 NTU, 14.10 mg/L and 8.90 mg/L respectively. Meanwhile, for ASHTP at L Block, the removal efficiencies of turbidity, COD, and TSS were found to be 43.20, 41.90, and 51.61% respectively, in which the pH, temperature, turbidity, COD, and TSS in the treated effluent were 7.30, 27.90°C, 21.10 NTU, 58.10 mg/L and 18.00 mg/L respectively. Proper maintenance of sewage treatment plant is important to ensure its effectiveness as well as to prolong its lifespan.


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