Anammox process for nitrogen removal from anaerobically digested fish canning effluents

2006 ◽  
Vol 53 (12) ◽  
pp. 265-274 ◽  
Author(s):  
A. Dapena-Mora ◽  
J.L. Campos ◽  
A. Mosquera-Corral ◽  
R. Méndez
Keyword(s):  
Nitrogen Removal ◽  
Bacterial Population ◽  
Loading Rate ◽  
Population Distribution ◽  
Nitrogen Loading ◽  
Fish Analysis ◽  
Organic Carbon Content ◽  
High Nitrogen ◽  
N Concentration ◽  
Anammox Process

The Anammox process was used to treat the effluent generated in an anaerobic digester which treated the wastewater from a fish cannery once previously processed in a Sharon reactor. The effluents generated from the anaerobic digestion are characterised by their high ammonium content (700–1,000 g NH+4-N m−3), organic carbon content (1,000–1,300 g TOC m−3) and salinity up to 8,000–10,000 g NaCl m−3. In the Sharon reactor, approximately 50% of the NH+4-N was oxidised to NO−2-N via partial nitrification. The effluent of the Sharon step was fed to the Anammox reactor which treated an averaged nitrogen loading rate of 500 g N m−3· d−1. The system reached an averaged nitrogen removal efficiency of 68%, mainly limited due to the nonstoichiometric relation, for the Anammox process, between the ammonium and nitrite added in the feeding. The Anammox reactor bacterial population distribution, followed by FISH analysis and batch activity assays, did not change significantly despite the continuous entrance to the system of aerobic ammonium oxidisers coming from the Sharon reactor. Most of the bacteria corresponded to the Anammox population and the rest with slight variable shares to the ammonia oxidisers. The Anammox reactor showed an unexpected robustness despite the continuous variations in the influent composition regarding ammonium and nitrite concentrations. Only in the period when NO−2-N concentration was higher than the NH+4-N concentration did the process destabilise and it took 14 days until the nitrogen removal percentage decreased to 34% with concentrations in the effluent of 340 g NH+4-N m−3 and 440 g NO−2-N m−3, respectively. Based on these results, it seems that the Sharon–Anammox system can be applied for the treatment of industrial wastewaters with high nitrogen load and salt concentration with an appropriate control of the NO−2-N/NH+4-N ratio.

scholarly journals Performance of the anammox sequencing batch reactor treating synthetic and real landfill leachate

2018 ◽  
Vol 44 ◽  
pp. 00179 ◽  
Author(s):  
Mariusz Tomaszewski ◽  
Grzegorz Cema ◽  
Tomasz Twardowski ◽  
Aleksandra Ziembińska-Buczyńska
Keyword(s):  
Nitrogen Removal ◽  
Landfill Leachate ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Nitrogen Loading ◽  
Synthetic Wastewater ◽  
High Nitrogen ◽  
Removal Capacity ◽  
Anammox Activity ◽  
Anammox Process

The anaerobic ammonium oxidation (anammox) process is one of the most energy efficient and environmentally-friendly bioprocess for the treatment of the wastewater with high nitrogen concentration. The aim of this work was to study the influence of the high nitrogen loading rate (NLR) on the nitrogen removal in the laboratory-scale anammox sequencing batch reactor (SBR), during the shift from the synthetic wastewater to landfill leachate. In both cases with the increase of NLR from 0.5 to 1.1 – 1.2 kg N/m3d, the nitrogen removal rate (NRR) increases to about 1 kg N/m3d, but higher NLR caused substrates accumulation and affects anammox process efficiency. Maximum specific anammox activity was determined as 0.638 g N/g VSSd (NRR 1.023 kg N/m3d) and 0.594 g N/g VSSd (NRR 1.241 kg N/m3d) during synthetic and real wastewater treatment, respectively. Both values are similar and this is probably the nitrogen removal capacity of the used anammox biomass. This indicates, that landfill leachate did not influence the nitrogen removal capacity of the anammox process.

scholarly journals Autotrophic ammonia removal from landfill leachate using anaerobic membrane bioreactor

2017 ◽  
Author(s):  
S. Suneethi ◽  
Kurian Joseph
Keyword(s):  
Nitrogen Removal ◽  
Landfill Leachate ◽  
Membrane Bioreactor ◽  
Loading Rate ◽  
Ammonia Removal ◽  
Nitrogen Loading ◽  
Ammonium Oxidation ◽  
Anaerobic Membrane Bioreactor ◽  
N Removal ◽  
Anammox Process

Anaerobic Membrane Bioreactor (AnMBR) is an innovative high cell density system having complete biomass retention, high reactor loading and low sludge production and suitable for developing slow growing autotrophic bacterial cultures such as ANAMMOX. The Anaerobic Ammonium Oxidation (ANAMMOX) process is an advanced biological nitrogen removal removes ammonia using nitrite as the electron acceptor without oxygen. The NH4+-N in the landfill leachate that is formed due to the release of nitrogen from municipal solid waste (MSW), when discharged untreated, into the surface water can result in eutrophication, aquatic toxicity and emissions of nitrous oxide (N2O) to atmosphere. Besides, NH4+-N accumulation in landfills poses long term pollution issue with significant interference during post closure thereby requiring its removal prior to ultimate disposal into inland surface waters. The main objective of this study was to investigate the feasibility and treatment efficiency of treating landfill leachate (to check) for removing NH4+-N by adopting ANAMMOX process in AnMBR. The AnMBR was optimized for Nitrogen Loading Rate (NLR) varying from 0.025 to 5 kg NH4+-N/ m3/ d with hydraulic retention time (HRT) ranging from 1 to 3 d. NH4+-N removal efficacy of 85.13 ± 9.67% with the mean nitrogen removal rate (NRR) of 5.54 ± 0.63 kg NH4+-N/ m3/ d was achieved with nitrogen loading rate (NLR) of 6.51 ± 0.20 kg NH4+- N/ m3/ d at 1.5 d HRT. The nitrogen transformation intermediates in the form of hydrazine (N2H4) and hydroxylamine (NH2OH) were 0.008 ± 0.005 mg/L and 0.006 ± 0.001 mg/L, respectively, indicating co-existence of aerobic ammonia oxidizers (AOB) and ANAMMOX. The free ammonia (NH3) and free nitrous acid (HNO2) concentrations were 26.61 ± 16.54 mg/L and (1.66 ± 0.95) x 10-5 mg/L, preventing NO2--N oxidation to NO3--N enabling sustained NH4+- N removal.

Nitrogen removal from piggery waste using the combined SHARON and ANAMMOX process

2005 ◽  
Vol 52 (10-11) ◽  
pp. 487-494 ◽  
Author(s):  
I.S. Hwang ◽  
K.S. Min ◽  
E. Choi ◽  
Z. Yun
Keyword(s):  
Nitrogen Removal ◽  
Ammonium Nitrogen ◽  
Steady States ◽  
Nitrogen Loading ◽  
Fish Analysis ◽  
Removal Ratio ◽  
N Removal ◽  
Conversion Rates ◽  
Anammox Process

Nitrogen removal in piggery waste was investigated with the combined SHARON-ANAMMOX process. The piggery waste was characterized as strong nitrogenous wastewater with very low C/N ratio. For the preceding SHARON reactor, ammonium nitrogen loading and conversion rates were 0.97kg NH4-N/m3reactor/day and 0.73kg NH4-N/m3 reactor/day, respectively. Alkalinity consumption for ammonium conversion was 8.5gr bicarbonate utilized per gram ammonium nitrogen converted to NO2-N or NO3-N at steady-states operation. The successive ANAMMOX reactor was fed with the effluent from SHARON reactor. Nitrogen loading and conversion rates were 1.36kg soluble N/m3 reactor/day and 0.72kg soluble N/m3 reactor/day, respectively. The average NO2-N/NH4-N removal ratio by ANAMMOX reaction was 2.13. It has been observed that Candidatus “Kuenenia stuttgartiensis” were dominated in the ANAMMOX reactor based on FISH analysis.

scholarly journals Anammox biofilm process using sugarcane bagasse as an organic carrier

2021 ◽  
Vol 26 (1) ◽  
pp. 25
Author(s):  
Zulkarnaini Zulkarnaini ◽  
Puti Sri Komala ◽  
Arief Almi
Keyword(s):  
Sugarcane Bagasse ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Nitrogen Loading ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Ammonium Oxidation ◽  
Biofilm Process ◽  
Anaerobic Sludge Blanket ◽  
Anammox Process

The anaerobic ammonium oxidation (anammox) biofilm process commonly uses various inorganic carriers to enhance nitrogen removal under anaerobic conditions. This study aims to analyze the performance of nitrogen removal in anammox process using sugarcane bagasse as an organic carrier. The experiment was carried out by using an up‐flow anaerobic sludge blanket (UASB) reactor for treating artificial wastewater at room temperature. The reactor was fed with ammonium and nitrite with the concentrations of 70‐150 mg–N/L and variations in the hydraulic retention time of 24 and 12 h. The granular anammox belongs to the genus Candidatus Brocadia sinica that was added as an inoculum of the reactor operation. The experimental stoichiometric of anammox for ΔNO2‐–N: ΔNH4+–N and ΔNO3‐: ΔNH4+ were 1.24 and 0.18, respectively, which is similar to anammox stoichiometry. The maximum Nitrogen Removal Rate (NRR) has achieved 0.29 kg–N/m3.d at Nitrogen Loading Rate (NLR) 0.6 kg–N/m3.d. The highest ammonium conversion efficiency (ACE) and nitrogen removal efficiency (NRE) were 88% and 85%, respectively. Based on this results, it indicated that sugarcane bagasse as organic carriers could increase the amount of total nitrogen removal by provided of denitrification process but inhibited the anammox process at a certain COD concentration.

High nitrogen removal rate using ANAMMOX process at short hydraulic retention time

2013 ◽  
Vol 67 (5) ◽  
pp. 968-975 ◽  
Author(s):  
C. G. Casagrande ◽  
A. Kunz ◽  
M. C. De Prá ◽  
C. R. Bressan ◽  
H. M. Soares
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Ammonium Oxidation ◽  
High Nitrogen ◽  
Total N ◽  
Column Reactor ◽  
Anaerobic Sludge Blanket ◽  
Anammox Process

The anaerobic ammonium oxidation (ANAMMOX) is a chemolithoautotrophic process, which converts NH4+ to N2 using nitrite (NO2−) as the electron acceptor. This process has very high nitrogen removal rates (NRRs) and is an alternative to classical nitrification/denitrification wastewater treatment. In the present work, a strategy for nitrogen removal using ANAMMOX process was tested evaluating their performance when submitted to high loading rates and very short hydraulic retention times (HRTs). An up-flow ANAMMOX column reactor was inoculated with 30% biomass (v v−1) fed from 100 to 200 mg L−1 of total N (NO2−-N + NH4+-N) at 35 °C. After start-up and process stability the maximum NRR in the up-flow anaerobic sludge blanket (UASB) reactor was 18.3 g-N L−1 d−1 operated at 0.2 h of HRT. FISH (fluorescence in situ hybridization) analysis and process stoichiometry confirmed that ANAMMOX was the prevalent process for nitrogen removal during the experiments. The results point out that high NRRs can be obtained at very short HRTs using up-flow ANAMMOX column reactor configuration.

Assessment of Inocula and N-Removal Performance of Anaerobic Ammonium Oxidation (ANAMMOX) for the Treatment of Aged Landfill Leachates

2012 ◽  
Vol 518-523 ◽  
pp. 2391-2398
Author(s):  
Yan He ◽  
Gong Ming Zhou ◽  
Min Sheng Huang ◽  
Min Tong
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Granular Sludge ◽  
Nitrogen Loading ◽  
Anaerobic Granular Sludge ◽  
Landfill Leachates ◽  
N Removal ◽  
Start Up ◽  
Anammox Process

Three kinds of seeding sludge, i.e. conventional activated sludge, anaerobic granular sludge and the nitrifying activated sludge from the nitritation reactor treating aged leachates were evaluated in batch mode to screen the optimized inoculum for the rapid start-up of ANAMMOX reactor. The feasibility of the ANAMMOX process for the treatment of aged leachates was also investigated in a modified upflow anaerobic sludge blanket (UASB, 0.05m3). The batch experiments revealed that the nitrifying activated sludge from the nitritation reactor could respectively achieve the NRR (nitrogen removal rate) of 0.0365 kg N/(m3.d) and the ARR (ammonium removal rate) of 0.013 kg N/(m3.d) on day 12, which were greatly higher than those of the other two tested sludge samples. The mixture of the aforementioned nitrifying activated sludge and anaerobic granular sludge was established as an effective inoculum for the prompt start-up of ANAMMOX reactor. The maximum total nitrogen removal rate of 0.826 kg N/(m3.d) could be obtained for the treatment of “old” leachates under NLR (nitrogen loading rate) of 1.028 kg N/(m3.d). It is concluded that the N-removal performance of ANAMMOX process is still to be improved for actual engineering application to aged landfill leachates.

A membrane bioreactor for an innovative biological nitrogen removal process

2010 ◽  
Vol 61 (3) ◽  
pp. 671-676 ◽  
Author(s):  
W. Chen ◽  
F. Y. Sun ◽  
X. M. Wang ◽  
X. Y. Li
Keyword(s):  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Loading Rate ◽  
Nitrogen Loading ◽  
Synthetic Wastewater ◽  
Biological Nitrogen Removal ◽  
Laboratory System ◽  
Working Volume ◽  
Biological Nitrogen

A hybrid system has been developed for biological nitrogen removal through nitrification-denitrification. The system includes an aerobic tank and an anoxic tank with an intermediate sludge settler connected to a membrane bioreactor (MBR) with a submerged 0.4 μm hollow-fiber membrane module. The laboratory system has a total working volume of 6.5 L treating a glucose-based synthetic wastewater. The experimental results demonstrate that the new process is highly effective for simultaneous organic and nitrogen removal. During the stationary operation, a sludge SS (suspended solids) concentration of 6 g/L or higher can be maintained in the reactors. The system has a COD (chemical oxygen demand) loading rate of up to 2,100 mg/L-d and a total nitrogen loading rate of up to 170 mg N/L-d. More than 95% COD can be degraded, and the total nitrogen removal efficiency can be 90% or higher as the nitrogen is reduced from 100 to around 7.5 mg/L. A high quality effluent is produced with a SS of less than 1 mg/L. With the MBR, organic degradation, nitrogen removal and sludge-liquid separation can be well achieved within a short HRT of about 10 hr.

scholarly journals Aplikasi Proses Anammox Dalam Penyisihan Nitrogen Menggunakan Reaktor Up-Flow Anaerobic Sludge Blanket

2020 ◽  
Vol 21 (1) ◽  
pp. 31-39
Author(s):  
Zulkarnaini Zulkarnaini ◽  
Reri Afrianita ◽  
Ilham Hagi Putra
Keyword(s):  
Nitrogen Removal ◽  
Nitrogen Loading ◽  
Laboratory Scale ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Stable Operation ◽  
Start Up ◽  
Anaerobic Sludge Blanket ◽  
Anammox Process

ABSTRACTAnammox process is a more practical alternative in biological nitrogen removal compared to conventional nitrification-denitrification processes. This process conducted at the optimum temperature of 370C. Indonesia, as a tropical country, has the potential for the application of anammox processes to remove nitrogen in wastewater. The purpose of this study was to analyze the efficiency of nitrogen removal in the anammox process using the Up-Flow Anaerobic Sludge Blanket (UASB) reactor at ambient temperature with variations in the hydraulic retention time (HRT) of 24 hours and 12 hours, at the laboratory scale. Samples are measured twice a week using a UV-Vis spectrophotometer. As a seeding sludge for start-up, the reactor was inoculated with granular anammox bacteria genus Candidatus Brocadia. At the stable operation, the ratio of ΔNO2--N:ΔNH4+-N and ΔNO3--N:ΔNH4+-N approach the stoichiometry of the anammox process were 1.20 and 0.21, respectively. The performance of nitrogen removal with 24-hour HRT obtained a maximum nitrogen removal rate (NRR) of 0.113 kg-N/m3.d with nitrogen loading rate (NLR) 0.14 kg-N/m3.d, and at 12-hour HRT, maximum NRR  of 0.196 kg-N/m3.d with NLR 0,28 kg-N/m3.d. Ammonium Conversion Efficiency (ACE) and Nitrogen Removal Efficiency (NRE) maximum for HRT 24 hours were 82% and 77%, respectively while HRT 12 hours were 72% and 68%, respectively. The anammox process operated stably in the tropical temperature with a temperature range of 23-280C on a laboratory scale using the UASB reactor.Keywords: anammox, nitrogen, temperature, tropical, uasb.ABSTRAKProses anammox menjadi alternatif yang lebih efektif dalam penyisihan nitrogen secara biologi dibandingkan dengan proses konvensional nitrifikasi-denitrifikasi. Proses ini berlangsung optimum pada suhu 370C. Indonesia sebagai negara tropis memiliki potensi untuk aplikasi proses anammox untuk menghilangkan nitrogen pada air limbah. Penelitian ini bertujuan untuk menganalisis efesiensi penyisihan nitrogen pada proses anammox menggunakan Up-Flow Anaerobic Sludge Blanket (UASB) reaktor pada suhu ambien dengan variasi Waktu Tinggal Hidrolik (WTH) 24 jam dan 12 jam, pada skala laboratorium. Sampel diukur dua kali setiap minggu menggunakan spektrofotometer UV-Vis. Sebagai seeding sludge (lumpur biakan) untuk start-up (memulai) reaktor digunakan bakteri anammox genus Candidatus Brocadia berbentuk granular. Berdasarkan hasil pengukuran, didapatkan nilai rasio ΔNO2--N:ΔNH4+-N dan ΔNO3--N:ΔNH4+-N mendekati stoikiometri proses anammox yaitu 1,20 dan 0,21. Kinerja penyisihan nitrogen dengan WTH 24 jam didapatkan nilai tingkat penyisihan nitrogen (TPyN ) maksimum 0,113 kg-N/m3.h pada tingkat pemuatan nitrogen (TPN) 0,14 kg-N/m3.h, dan WTH 12 jam nilai TPyN  maksimum 0,196 kg-N/m3.h pada TPN 0,28 kg-N/m3.h. Nilai efisiensi konversi amonia (EKA) dan efisiensi penyisihan nitrogen (EPN) maksimum pada WTH 24 jam berturut-turut adalah 82% dan 77%, sedangkan pada WTH 12 jam berturut-turut adalah 72% dan 68%. Penelitian membuktikan bahwa proses anammox dapat berlangsung stabil pada daerah tropis dengan suhu terukur 21-290C pada skala laboratorium menggunakan UASB reaktor. Kata kunci: Anammox, nitrogen, temperatur, tropis, uasb.

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