Environmental dissemination of group A rotavirus: P-type, G-type and subgroup characterization

2009 ◽  
Vol 60 (3) ◽  
pp. 633-642 ◽  
Author(s):  
F. F. M. Ferreira ◽  
F. R. Guimarães ◽  
T. M. Fumian ◽  
M. Victoria ◽  
C. B. Vieira ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Health Hazard ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Northern Region ◽  
Rt Pcr ◽  
Vp6 Gene ◽  
Elution Method ◽  
P Type

Rotaviruses A (RV-A) infection is the most common cause of acute diarrheal diseases in infants and the dissemination of these viruses in the environment represents a public health hazard. The present study aims to evaluate reverse transcription-polymerase chain reaction (RT-PCR) based protocols for the detection of RV-A genes in different types of environmental samples. RV-A were concentrated by the adsorption-elution method using negatively charged membranes associated with a Centriprep Concentrator 50. The RV-A VP4, VP7 and VP6 genes were detected using RT-PCR in river water from the Amazon Hydrographic basin (Northern region) and from wastewater in a sewage treatment plant in Rio de Janeiro (Southeast region), Brazil. RV-A were successfully detected in water environmental samples by the methods used. The detection of the VP6 gene by RT-PCR was the most sensitive for detecting RV-A in environmental samples (44.0%), when compared to the detection of the VP4 (33.3%) and VP7 (25.3%) genes. Based on nucleotide sequence and phylogenetic analysis of the partial VP6 gene, 22 environmental samples were determined to be subgroup II (Wa-like). These results indicate that analysis of environmental samples could possibly make a valuable contribution to studies on the epidemiology of RV-A.

scholarly journals An epidemiological study of enteric viruses in sewage with molecular characterization by RT-PCR and sequence analysis

2008 ◽  
Vol 6 (3) ◽  
pp. 351-358 ◽  
Author(s):  
A. Arraj ◽  
J. Bohatier ◽  
C. Aumeran ◽  
J. L. Bailly ◽  
H. Laveran ◽  
...  
Keyword(s):  
Hepatitis A Virus ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Enteric Viruses ◽  
Sample Volume ◽  
Small Sample ◽  
Detection Methods ◽  
Rt Pcr ◽  
Treated Effluent

The aim of this study was to assess the presence and seasonal frequency of various enteric viruses in wastewater treatment. The detection of astrovirus, norovirus, enterovirus, hepatitis A virus (HAV) and rotavirus was carried out by molecular analyses in concentrated water samples collected over 18 months at the entrance and exit of an activated sludge sewage treatment plant. The reverse transcriptase-polymerase chain reaction (RT-PCR) results were confirmed by sequencing, and comparative phylogenetic analysis was performed on the isolated strains. Genomes of human astrovirus and human rotavirus were identified in 26/29 and 11/29 samples of raw sewage, respectively, and in 12/29 and 13/29 treated effluent samples, respectively. Some rotavirus sequences detected in environmental samples were very close to those of clinical strains. Noroviruses, enteroviruses and HAV were not detected during the study period. This could be related to the small sample volume, to the sensitivity of the detection methods or to local epidemiological situations. Frequent detection of viral RNA, whether infectious or not, in the exit effluent of sewage treatment indicates wide dispersion of enteric viruses in the environment. Consequently, viral contamination resulting from the use of these treated waters is a risk that needs to be addressed.

Analysis and balance of phosphorous removal from wastewater at urban wastewater treatment plant

2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Phosphorus Recovery ◽  
Anaerobic Sludge ◽  
Biological Phosphorus Removal ◽  
The Mean

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.

Changes of microbial indices of water quality in the Vistula and Brda rivers as a result of sewage treatment plant operation

2008 ◽  
Vol 37 (2) ◽  
Author(s):  
Maciej Walczak
Keyword(s):  
Water Quality ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Sewage Treatment Plants ◽  
Plant Operation ◽  
Treated Sewage ◽  
Microbiological Parameters ◽  
Microbial Indices

Changes of microbial indices of water quality in the Vistula and Brda rivers as a result of sewage treatment plant operationThis paper reports the results of studies of microbiological changes in the water quality of the Vistula and Brda rivers after the opening of sewage treatment plants in Bydgoszcz. The study involved determining the microbiological parameters of water quality. Based on the results obtained, it was found that the quality of the water in both rivers had improved decidedly after the opening of the plants, although an increased number of individual groups of microorganisms was found at the treated sewage outlet from one of the plants.

Properties of Nocardiaform Actinomycetes Isolated from a Sewage Treatment Plant of Southern Part of Miyagi.

2000 ◽  
Vol 36 (4) ◽  
pp. 161-171
Author(s):  
KENITSU KONNO ◽  
NAOKI ABE ◽  
YOSHIRO SATO ◽  
KOJI AKAMATSU ◽  
MAKOTO ABE ◽  
...  
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant

Effect of Wastewater on the Soil and Irrigation Process: A Laboratory Study

2017 ◽  
Vol 1 (1) ◽  
pp. 46-55 ◽  
Author(s):  
Athar Hussain ◽  
Manjeeta Priyadarshi ◽  
Saif Said ◽  
Suraj Negi
Keyword(s):  
Heavy Metals ◽  
Tap Water ◽  
Sewage Treatment ◽  
Daily Intake ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Surface Water Resources ◽  
Industrial Sewage ◽  
Concentration Levels

Most of the industrial sewage effluents used for irrigation contains heavy metals which cause toxicity to crop plants as the soils are able to accumulate heavy metal for many years. The vegetables grown for the present study were irrigated with treated wastewater brought from a nearby full-scale sewage treatment plant at different compositions along with tap water as a control. The concentration levels of the Cd, Co, Cu, Mn and Zn in the soil were found to below the toxic limits as prescribed in literature. Daily Intake Metals (DIM) values suggest that the consumption of plants grown in treated wastewater and tap water is nearly free of risks, as the dietary intake limits of Cu, Fe, Zn and Mn. The Enrichment Factor for the treated wastewater irrigated soil was found in order Zn> Ni> Pb> Cr> Cu> Co> Mn> Cd. Thus, treated wastewater can be effectively used for irrigation. This will have twofold significant environmental advantages: (1) helpful to reduce the groundwater usage for irrigation and (2) helpful to reduce the stress on surface water resources.

Spatial and Temporal Water Quality Trends in Severn Sound, Georgian Bay, since the Introduction of Phosphorus Control Guidelines: Nutrients and Phytoplankton, 1973 to 1991

1995 ◽  
Vol 30 (4) ◽  
pp. 565-592 ◽  
Author(s):  
A.F. Gemza
Keyword(s):  
Total Phosphorus ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Soluble Reactive Phosphorus ◽  
Euphotic Zone ◽  
Water Clarity ◽  
Sewage Treatment Plants ◽  
Order Of Magnitude ◽  
Phosphorus Levels

Abstract Severn Sound continues to exhibit signs of eutrophication despite initial identification of the problem in 1969 and the construction of several sewage treatment plants since then. In general, improvements in trophic state indicators have been marginal, suggesting that the sewage treatment plants have had limited success in controlling phosphorus concentrations. These discharges likely contributed to the increased total phosphorus levels and consequently the higher phytoplankton densities of the nearshore waters. Phytoplankton biovolumes were on average one order of magnitude higher than in the open waters of Lake Huron with mean summer biovolumes as high as 8.0 mm/L. Algal biovolumes were most dense in Penetang Bay, which experienced limited exchange with the main waters of the sound. No significant long-term trends were observed. Water clarity was declining significantly, however, at a rate of -0.60 to -0.78 m/year throughout the sound except in Sturgeon Bay. Total phosphorus levels were highly variable from year to year; however, concentrations from a 20-year perspective were declining in the open waters at a rate of 0.70 µg/L/year, but response was limited in nearshore areas. In Sturgeon Bay, mean annual euphotic zone total phosphorus as well as soluble reactive phosphorus levels declined by as much as 50% following the construction of a sewage treatment plant with tertiary treatment. Phytoplankton genera typical of eutrophic waters continued to dominate the algal assemblage but members indicative of mesotrophic conditions have become apparent in some areas of the sound.

Control of Bulking in an Extended Aeration Sewage Treatment Plant

1972 ◽  
Vol 7 (1) ◽  
pp. 13-20
Author(s):  
D.D.P. Cane ◽  
G.J. Farguhar
Keyword(s):  
Dissolved Oxygen ◽  
Control Measure ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Effective Control ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Mixed Liquor ◽  
Air Supply

Abstract A case of filamentous activated sludge bulking was investigated at an extended aeration plant treating wastes from a highway service centre. The purpose of the study was to find an effective control measure for the bulking and, if possible, to determine the cause of the bulking condition. Experiments were conducted to determine the effects upon bulking of: (a) a controlled copper dosage to the mixed liquor, (b) variations in the organic loading rate, and (c) maintenance of high and low dissolved oxygen concentrations in the mixed liquor. The microorganism responsible for the bulking condition was tentatively identified as Sphaerotilus natans. Extensive growths of these bacteria occurred in the sludge when the mixed liquor dissolved oxygen concentration dropped below 0.5 mg/1. Such dissolved oxygen levels had frequently occurred at this plant due to the use of timed aeration cycles and the use of insufficient air supply during peak loading periods. Variations in organic loading rates were found to have no adverse effect on sludge settleability. When bulking was slight, the condition could be improved by increasing the dissolved oxygen level to 1.5 mg/1, or more. The rate at which the filamentous growths could be eliminated from a highly filamentous sludge by increased aeration was very slow, but could be greatly increased by the simultaneous feeding of copper to the mixed liquor at a dosage rate of 1.0 mg/1, based on the raw sewage flows.

Sign in / Sign up

Export Citation Format

Share Document