scholarly journals Simultaneous phosphorus uptake and denitrification by EBPR-r biofilm under aerobic conditions: effect of dissolved oxygen

2015 ◽  
Vol 72 (7) ◽  
pp. 1147-1154 ◽  
Author(s):  
Pan Yu Wong ◽  
Maneesha P. Ginige ◽  
Anna H. Kaksonen ◽  
Ralf Cord-Ruwisch ◽  
David C. Sutton ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Aerobic Denitrification ◽  
Biological Phosphorus Removal ◽  
Denitrification Activity ◽  
Biofilm Process ◽  
Oxygen Intrusion ◽  
Biofilm Structure ◽  
Biological Phosphorus

A biofilm process, termed enhanced biological phosphorus removal and recovery (EBPR-r), was recently developed as a post-denitrification approach to facilitate phosphorus (P) recovery from wastewater. Although simultaneous P uptake and denitrification was achieved despite substantial intrusion of dissolved oxygen (DO >6 mg/L), to what extent DO affects the process was unclear. Hence, in this study a series of batch experiments was conducted to assess the activity of the biofilm under various DO concentrations. The biofilm was first allowed to store acetate (as internal storage) under anaerobic conditions, and was then subjected to various conditions for P uptake (DO: 0–8 mg/L; nitrate: 10 mg-N/L; phosphate: 8 mg-P/L). The results suggest that even at a saturating DO concentration (8 mg/L), the biofilm could take up P and denitrify efficiently (0.70 mmol e−/g total solids*h). However, such aerobic denitrification activity was reduced when the biofilm structure was physically disturbed, suggesting that this phenomenon was a consequence of the presence of oxygen gradient across the biofilm. We conclude that when a biofilm system is used, EBPR-r can be effectively operated as a post-denitrification process, even when oxygen intrusion occurs.

Biological Phosphorus Removal in a Sequencing Batch Moving Bed Biofilm Reactor

1999 ◽  
Vol 40 (4-5) ◽  
pp. 161-168 ◽  
Author(s):  
H. Helness ◽  
H. Ødegaard
Keyword(s):  
Phosphorus Removal ◽  
Batch Reactor ◽  
Complete Removal ◽  
Biofilm Reactor ◽  
Biological Phosphorus Removal ◽  
Moving Bed Biofilm Reactor ◽  
Moving Bed ◽  
Biofilm Carrier ◽  
Biofilm Process ◽  
Biological Phosphorus

Experiments have been carried out with biological phosphorus removal in a sequencing batch moving bed biofilm reactor (SBMBBR) with a plastic biofilm carrier (Kaldnes) suspended in the wastewater. The aim of the research leading to this paper was to evaluate biological phosphorus removal in this type of biofilm process. Biological phosphorus removal can be achieved in a moving bed biofilm reactor operated as a sequencing batch reactor. In order to achieve good and stable phosphorus removal over time, the length of the anaerobic period should be tuned to achieve near complete removal of easily biodegradable COD in the anaerobic period. The total COD-loading rate must at the same time be kept high enough to achieve a net growth of biomass in the reactor. Use of multivariate models based on UV-absorption spectra and measurements of the redox potential show potential for control of such a process.

Phosphorus Uptake by Klebsiella Pneumoniae and Acinetobacter Calcoaceticus

1985 ◽  
Vol 17 (11-12) ◽  
pp. 113-118 ◽  
Author(s):  
R. M. Gersberg ◽  
D. W. Allen
Keyword(s):  
Klebsiella Pneumoniae ◽  
Municipal Wastewater ◽  
Phosphorus Uptake ◽  
High Capacity ◽  
Acinetobacter Calcoaceticus ◽  
P Uptake ◽  
Cell System ◽  
Biological Phosphorus Removal ◽  
Immobilized Cell ◽  
Suspended Growth

The objective of our study was to show that pure cultures of Klebsiella pneumoniae and Acinetobacter calcoaceticus could be Induced to accumulate large amounts of phosphorus (P), when P-starved cultures were enriched with phosphorus either in suspended growth or immobilized cell reactors. Suspended growth cultures of K. pneumoniae were more efficient than those of A. calcoaceticus, with specific uptake rates of 14.1 - 17.1 mg P1−1 hr−1 per O.D. unit, and 5.4 - 10.0 mg P1−1 hr −1 per O.D. unit, respectively. The absolute rate of P accumulation of 24.6 mg P1−1 hr−1 measured for a K. pneumoniae culture was among the highest ever reported in the literature. In an immobilized cell system, which facilitates the separation of the cells (for recycling) from the liquid phase, K. pneumoniae cells entrapped in agar gel beads, remained viable and showed rates of P uptake of 6.1 and 7.9 mg P1−1 hr−1. K. pneumoniae cultures also showed a high capacity for removing dissolved phosphate from municipal wastewater, with greater than 95% P removal in two hours. These studies suggest the important role such high-phosphate accumulating bacteria may play in wastewater treatment systems designed for enhanced biological phosphorus removal.

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

An enhanced biological phosphorus removal (EBPR) control strategy for sequencing batch reactors (SBRs)

2001 ◽  
Vol 43 (3) ◽  
pp. 183-189 ◽  
Author(s):  
C. Y. Dassanayake ◽  
R. L. Irvine
Keyword(s):  
Control Strategy ◽  
Phosphorus Removal ◽  
Batch Reactor ◽  
Past Research ◽  
P Uptake ◽  
Batch Reactors ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Aeration Time ◽  
Biological Phosphorus

A control strategy was developed for enhanced biological phosphorus removal (EBPR) in a Sequencing Batch Reactor (SBR). Unlike past research that focused on maximizing polyhdroxyalkanoate (PHA) formation during the anaerobic period, this study investigated some of the factors that govern aerobic PHA dynamics and its efficient regulation during phosphate (P) uptake. Influent COD, influent P, and the time for aeration were critical factors that governed PHA use and P uptake during aerated react. Unnecessary PHA oxidation (i.e., in the absence of extracellular P) occurred if the time for aerated react exceeded the time required for P uptake. By adjusting the aeration time to that required for P uptake, residual PHA was sustained in the SBR and excess phosphate uptake reaction potential (PRP) was generated for use during transient influent excursions in P. Unlike space oriented systems, the time for react is simply adjusted in the SBR. Because residual PHA is easily maintained once achieved, high influent COD events can be harnessed to increase or sustain excess PRP for management of expected variations in influent P.

Phosphorus release and uptake during start-up of a covered and non-aerated sequencing batch reactor with separate feeding of VFA and sulfate

2012 ◽  
Vol 65 (5) ◽  
pp. 840-844 ◽  
Author(s):  
D. Wu ◽  
T. Hao ◽  
H. Lu ◽  
H. K. Chui ◽  
M. C. M. van Loosdrecht ◽  
...  
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
P Uptake ◽  
Sulfur Cycle ◽  
Biological Phosphorus Removal ◽  
P Release ◽  
Start Up ◽  
Uptake Rates ◽  
Biological Phosphorus ◽  
P Removal

This study explored a sulfur cycle-associated biological phosphorus (P) removal process in a covered and non-aerated sequencing batch reactor (SBR) fed with volatile fatty acid (VFA) and sulfate separately. During the 60-day start-up, both phosphate release and uptake rates increased, while poly-phosphate cyclically increased and decreased accordingly. The P-release and P-uptake rates were associated with VFA uptake and sulfate reduction. The average ratio of potassium to phosphate during the P-uptake and P-release was also determined to be 0.29–0.31 mol K/mol P, which is close to a reported value (0.33) for biological phosphorus removal. All this evidence confirmed there was biological P removal in this reactor, in which metabolism could be different from conventional biological P removal.

Combined denitrification and excess biological phosphorus removal in discontinuous operated biofilm systems

2002 ◽  
Vol 46 (4-5) ◽  
pp. 193-200 ◽  
Author(s):  
D. Brandt ◽  
C. Sieker ◽  
W. Hegemann
Keyword(s):  
Phosphorus Removal ◽  
P Uptake ◽  
Organic Substrate ◽  
Treated Wastewater ◽  
Biological Phosphorus Removal ◽  
P Release ◽  
Different Types ◽  
Immobilized Biomass ◽  
Biological Phosphorus ◽  
P Removal

The sorption-denitrification-P-removal (S-DN-P) process combines biological excess P-removal (BEPR) and denitrification using immobilized biomass. The accumulation of denitrifying polyP organisms is achieved by sequencing anaerobic/anoxic conditions. The immobilized biomass is in alternating contact with primary treated wastewater (anaerobic sorption-phase) and nitrified wastewater (denitrification phase). In the sorption phase, P-release takes place and readily biodegradable organic substrate, e.g. volatile fatty acid, is taken up and stored by polyP accumulating organisms (PAO). In addition to this, other organic matter is physically/chemically adsorbed in the biofilm structures. In the denitrification phase, the biomass denitrifies the stored and adsorbed organic substrate and, at the same time, P-uptake and polyP formation occurs. This paper presents results of investigations at laboratory and half-technical scale. At laboratory scale different types of carriers were tested regarding their suitability for the S-DN-P-process. In half-technical scale a biofilter and a moving bed reactor (MBR) were tested. In the biofilter a stable removal of nitrate and phosphate was achieved. However, it was not possible to achieve similar results in the MBR process. Especially the release and uptake of phosphate showed no clear tendency although the uptake of acetate was good. Reasons for this could be the accumulation of glycogen accumulating organisms which impair the metabolism of PAO.

Effect of continuous addition of an organic substrate to the anoxic phase on biological phosphorus removal

1998 ◽  
Vol 38 (1) ◽  
pp. 97-105 ◽  
Author(s):  
J. Meinhold ◽  
H. Pedersen ◽  
E. Arnold ◽  
S. Isaacs ◽  
M. Henze
Keyword(s):  
Phosphorus Removal ◽  
Phosphate Uptake ◽  
P Uptake ◽  
Organic Substrate ◽  
Organic Substrates ◽  
Biological Phosphorus Removal ◽  
P Release ◽  
Influence Of Substrate ◽  
Biological Phosphorus ◽  
P Removal

The continuous introduction of a biological phosphorus removal (BPR) promoting organic substrate to the denitrifying reactor of a BPR process is examined through a series of batch experiments using acetate as model organic substrate. Several observations are made regarding the influence of substrate availability on PHA storage/utilization and phosphate uptake/release. Under anoxic conditions PHB is utilized and phosphate is taken up, indicating that at least a fraction of the PAO can denitrify. The rates of anoxic P-uptake, PHB utilization and denitrification are found to increase with increasing initial PHB level. At low acetate addition rates the P-uptake and PHB utilization rates are reduced compared to when no acetate is available. At higher acetate addition rates a net P-release occurs and PHB is accumulated. For certain intermediate acetate addition rates the PHB level can increase while a net P-release occurs. Whether the introduction of BPR promoting organic substrates to the denitrifying reactor is detrimental to overall P-removal appears to be dependent on the interaction between aerobic P-uptake, which is a function of PHB level, and the aerobic residence time.

Biological phosphorus uptake in submerged biofilters with nitrogen removal

1994 ◽  
Vol 29 (10-11) ◽  
pp. 135-143 ◽  
Author(s):  
R. F. Gonçalves ◽  
L. Le Grand ◽  
F. Rogalla
Keyword(s):  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Treatment Plant ◽  
Pilot Scale ◽  
Biological Phosphorus Removal ◽  
Low Phosphorus ◽  
Nitrification And Denitrification ◽  
Aerated Filter ◽  
Filter Bed ◽  
Biological Phosphorus

This paper introduces biological phosphorus removal (Bio-P) from wastewater on a submerged biofilter. Pilot scale research was carried out over a period of two years using a floating upflow aerated filter, originally designed for nitrification and denitrification of sewage. The factors which influence Bio-P on fixed film processes and the possible biofilter configurations which eliminate C, N and P are discussed. The procedures are applicable to all types of treatment plants using biofilters, both new and already in existence, making no distinction between the different processes available today, co-current and counter-current filters. Biological phosphorus removal can be associated to the different treatment levels required: organic matter removal; secondary nitrification secondary nitrification and denitrification. For the third option - complete nutrient removal, treatment is completed with a hydraulic retention time in the filter bed of under four hours. Because of the simultaneous filtration with effluent SS below 10 mg/l, low phosphorus residuals can be achieved by Bio-P alone. The modifications required for setting up this operating procedure on any treatment plant are presented.

scholarly journals Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

2013 ◽  
Vol 79 (6) ◽  
pp. 1969-1979 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Hyo Jung Lee ◽  
Dae Sung Lee ◽  
Che Ok Jeon
Keyword(s):  
Batch Reactor ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Biological Phosphorus Removal ◽  
Associated Bacteria ◽  
Content Type ◽  
Accumulibacter Phosphatis ◽  
Clear Shift ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

ABSTRACTTo characterize the denitrifying phosphorus (P) uptake properties of “CandidatusAccumulibacter phosphatis,” a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaerobic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of “Ca. Accumulibacter” and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescencein situhybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized “Ca. Accumulibacter” subpopulations from clades IIA > IA > IIF to clades IIC > IA > IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas[from 1.2% to 19.2%] and “CandidatusCompetibacter phosphatis” [from 16.4% to 20.0%]), while the overall “Ca. Accumulibacter” abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to characterize the denitrifying P uptake properties of the “Ca. Accumulibacter” clades. In FISH/MAR experiments using slightly diluted sludge (∼0.5 g/liter), all “Ca. Accumulibacter” clades successfully took up phosphorus in the presence of nitrate. However, the “Ca. Accumulibacter” clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (∼0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by “Ca. Accumulibacter” clades occurred when nitrite was added. These results suggest that the “Ca. Accumulibacter” cells lack nitrate reduction capabilities and that P uptake by “Ca. Accumulibacter” is dependent upon nitrite generated by associated nitrate-reducing bacteria such asDechloromonasand “Ca. Competibacter.”

Sign in / Sign up

Export Citation Format

Share Document