Phosphorus Uptake by Klebsiella Pneumoniae and Acinetobacter Calcoaceticus

1985 ◽  
Vol 17 (11-12) ◽  
pp. 113-118 ◽  
Author(s):  
R. M. Gersberg ◽  
D. W. Allen
Keyword(s):  
Klebsiella Pneumoniae ◽  
Municipal Wastewater ◽  
Phosphorus Uptake ◽  
High Capacity ◽  
Acinetobacter Calcoaceticus ◽  
P Uptake ◽  
Cell System ◽  
Biological Phosphorus Removal ◽  
Immobilized Cell ◽  
Suspended Growth

The objective of our study was to show that pure cultures of Klebsiella pneumoniae and Acinetobacter calcoaceticus could be Induced to accumulate large amounts of phosphorus (P), when P-starved cultures were enriched with phosphorus either in suspended growth or immobilized cell reactors. Suspended growth cultures of K. pneumoniae were more efficient than those of A. calcoaceticus, with specific uptake rates of 14.1 - 17.1 mg P1−1 hr−1 per O.D. unit, and 5.4 - 10.0 mg P1−1 hr −1 per O.D. unit, respectively. The absolute rate of P accumulation of 24.6 mg P1−1 hr−1 measured for a K. pneumoniae culture was among the highest ever reported in the literature. In an immobilized cell system, which facilitates the separation of the cells (for recycling) from the liquid phase, K. pneumoniae cells entrapped in agar gel beads, remained viable and showed rates of P uptake of 6.1 and 7.9 mg P1−1 hr−1. K. pneumoniae cultures also showed a high capacity for removing dissolved phosphate from municipal wastewater, with greater than 95% P removal in two hours. These studies suggest the important role such high-phosphate accumulating bacteria may play in wastewater treatment systems designed for enhanced biological phosphorus removal.

scholarly journals Simultaneous phosphorus uptake and denitrification by EBPR-r biofilm under aerobic conditions: effect of dissolved oxygen

2015 ◽  
Vol 72 (7) ◽  
pp. 1147-1154 ◽  
Author(s):  
Pan Yu Wong ◽  
Maneesha P. Ginige ◽  
Anna H. Kaksonen ◽  
Ralf Cord-Ruwisch ◽  
David C. Sutton ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Aerobic Denitrification ◽  
Biological Phosphorus Removal ◽  
Denitrification Activity ◽  
Biofilm Process ◽  
Oxygen Intrusion ◽  
Biofilm Structure ◽  
Biological Phosphorus

A biofilm process, termed enhanced biological phosphorus removal and recovery (EBPR-r), was recently developed as a post-denitrification approach to facilitate phosphorus (P) recovery from wastewater. Although simultaneous P uptake and denitrification was achieved despite substantial intrusion of dissolved oxygen (DO >6 mg/L), to what extent DO affects the process was unclear. Hence, in this study a series of batch experiments was conducted to assess the activity of the biofilm under various DO concentrations. The biofilm was first allowed to store acetate (as internal storage) under anaerobic conditions, and was then subjected to various conditions for P uptake (DO: 0–8 mg/L; nitrate: 10 mg-N/L; phosphate: 8 mg-P/L). The results suggest that even at a saturating DO concentration (8 mg/L), the biofilm could take up P and denitrify efficiently (0.70 mmol e−/g total solids*h). However, such aerobic denitrification activity was reduced when the biofilm structure was physically disturbed, suggesting that this phenomenon was a consequence of the presence of oxygen gradient across the biofilm. We conclude that when a biofilm system is used, EBPR-r can be effectively operated as a post-denitrification process, even when oxygen intrusion occurs.

Phosphorus and Nitrogen Removal in Moving-Bed Sequencing Batch Biofilm Reactors

1999 ◽  
Vol 40 (4-5) ◽  
pp. 169-176 ◽  
Author(s):  
Giuseppe Pastorelli ◽  
Roberto Canziani ◽  
Luca Pedrazzi ◽  
Alberto Rozzi
Keyword(s):  
Organic Carbon ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Phosphorus Uptake ◽  
Biofilm Reactor ◽  
Municipal Wastewater Treatment ◽  
Biological Phosphorus Removal ◽  
Nitrogen And Phosphorus ◽  
Limit Values ◽  
Moving Bed

A pilot moving-bed sequencing batch biofilm reactor (MBSBBR) fed with primary settled wastewater, was used in order to study organic carbon, phosphorus and nitrogen removal with and without external carbon sources. Patented KMT® polyethylene biofilm carriers were used. Organic carbon uptake and phosphorus release has been achieved in the anaerobic phase of the cycle, while nitrification, simultaneous denitrification (i.e., anoxic respiration of sequestered COD in the inner layer of the biofilm) and phosphorus uptake was observed in the aerobic phase. A stable biological phosphorus removal could be achieved only with an external carbon source. Since the process proved flexible and reliable, it is suitable for full scale application to municipal wastewater treatment plants (WWTPs), in order to meet EU total nitrogen and phosphorus limit values for discharge into sensitive receiving waters.

scholarly journals Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

2013 ◽  
Vol 79 (6) ◽  
pp. 1969-1979 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Hyo Jung Lee ◽  
Dae Sung Lee ◽  
Che Ok Jeon
Keyword(s):  
Batch Reactor ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Biological Phosphorus Removal ◽  
Associated Bacteria ◽  
Content Type ◽  
Accumulibacter Phosphatis ◽  
Clear Shift ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

ABSTRACTTo characterize the denitrifying phosphorus (P) uptake properties of “CandidatusAccumulibacter phosphatis,” a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaerobic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of “Ca. Accumulibacter” and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescencein situhybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized “Ca. Accumulibacter” subpopulations from clades IIA > IA > IIF to clades IIC > IA > IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas[from 1.2% to 19.2%] and “CandidatusCompetibacter phosphatis” [from 16.4% to 20.0%]), while the overall “Ca. Accumulibacter” abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to characterize the denitrifying P uptake properties of the “Ca. Accumulibacter” clades. In FISH/MAR experiments using slightly diluted sludge (∼0.5 g/liter), all “Ca. Accumulibacter” clades successfully took up phosphorus in the presence of nitrate. However, the “Ca. Accumulibacter” clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (∼0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by “Ca. Accumulibacter” clades occurred when nitrite was added. These results suggest that the “Ca. Accumulibacter” cells lack nitrate reduction capabilities and that P uptake by “Ca. Accumulibacter” is dependent upon nitrite generated by associated nitrate-reducing bacteria such asDechloromonasand “Ca. Competibacter.”

Biological phosphorus and nitrogen removal with biological aerated filter using denitrifying phosphorus accumulating organism

2005 ◽  
Vol 52 (10-11) ◽  
pp. 569-578 ◽  
Author(s):  
J. Lee ◽  
J. Kim ◽  
C. Lee ◽  
Z. Yun ◽  
E. Choi
Keyword(s):  
P Uptake ◽  
Biological Nutrient Removal ◽  
Biological Aerated Filter ◽  
Biological Phosphorus Removal ◽  
Nitrogen And Phosphorus ◽  
Suspended Growth ◽  
Oxic Zone ◽  
Aerated Filter ◽  
Biological Phosphorus ◽  
P Removal

In order to accomplish the biological nutrient removal with a weak sewage at low temperature, a hybrid process consisted of anoxic denitrifying phosphorus accumulating organism (dPAO) and nitrifying biological aerated filter (BAF) was studied in both lab and field pilot plants with weak sewage. The biofilm BAF was used as a post-nitrification process that provided sufficient nitrate to suspended growth dPAO. The anoxic/BAF configuration could remove nitrogen and phosphorus appreciably compared to other BNR systems. The enhanced biological phosphorus removal (EBPR) was mainly occurred in anoxic zone of suspended growth reactor. It has been found that P removal efficiency of dPAO was enhanced with an addition of a short oxic zone in suspended reactors compared to that of without oxic zone. However, the degree of aerobic P uptake in oxic zone was far lower than anoxic P uptake. The operating results of field plant indicated that dPAO/BAF configuration successfully reduced the adverse temperature effects at lower than 15°C.

Conversion of Existing Primary Clarifiers According to the EASC Process for Biological Phosphorus Removal

1990 ◽  
Vol 22 (7-8) ◽  
pp. 45-51 ◽  
Author(s):  
R. Schönberger
Keyword(s):  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Operation Mode ◽  
Phosphorus Uptake ◽  
Treatment Plant ◽  
Municipal Wastewater Treatment ◽  
Biological Phosphorus Removal ◽  
Northern Germany ◽  
Start Up ◽  
Biological Phosphorus

At the end of 1988 a 22,000 p.e. municipal wastewater treatment plant in Northern Germany was converted to the EASC-biological phosphorus removal process. By simple modifications of the flow scheme of the plant, one of two existing primary clarifiers was converted to an anaerobic basin, into which both sewage and recycle sludge are fed. The supernatant as well as the sludge withdrawn from the bottom are discharged into the aeration basin. This operation mode achieves very good phosphorus uptake in the aeration basin. Since start up in November '88, the uptake-capacity increased continually, since April '89 phosphorus is removed down to concentrations of less than 1 mg/l PO4-P in the aeration basin. Due to an inadequate design and size of the existing final clarifier, phosphorus bleedback occurs and reduces removal efficiency. This bleedback could be minimized by either intensifying denitrification or reducing sludge detention time in the final clarifier.

scholarly journals Phosphorus effects of recycled products from municipal wastewater on crops in a field experiment

2017 ◽  
Vol 63 (No. 10) ◽  
pp. 475-482 ◽  
Author(s):  
Vogel Telse ◽  
Nelles Michael ◽  
Eichler-Löbermann Bettina
Keyword(s):  
Field Experiment ◽  
Municipal Wastewater ◽  
Spring Barley ◽  
P Uptake ◽  
Control Treatment ◽  
First Year ◽  
Soil P ◽  
Second Year ◽  
Fertilizing Effect ◽  
Sludge Ash

In this study, the phosphorus (P) fertilizing effects of struvite, one thermochemical-treated sewage sludge ash (SSA) based on Ca-P (Ca-SSA) and one full sulfuric acid-digested SSA based on Al-P (Al-SSA) were analysed in comparison to triple superphosphate (TSP) and a control treatment (CON) without P application in a two-year field experiment. In the field experiment, the effects of the recycling products on crop yield, P uptake and labile soil P fractions were analysed. In addition, the effect of nitrogen and magnesium contained in struvite was investigated in the second year of the experiment compared to TSP and CON. In the first year, spring barley was cultivated in the field experiment; and in the second year, it was forage rye followed by sorghum. In the second year, the relative P effectiveness (forage rye, sorghum) of the recycling products compared to TSP increased in the order: Ca-SSA (81%, 91%) ≤ Al-SSA (91%, 96%) = struvite (102%, 110%). In addition, an magnesium fertilizing effect of struvite could be demonstrated. The results show that the recycling products from wastewater treatment are appropriate to substitute rock phosphate-based fertilizers.

The impact of biomass harvesting on phosphorus uptake by wetland plants

2001 ◽  
Vol 44 (11-12) ◽  
pp. 61-67 ◽  
Author(s):  
S-Y. Kim ◽  
P.M. Geary
Keyword(s):  
Plant Biomass ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Plant Parts ◽  
Biomass Harvesting ◽  
Below Ground ◽  
The Impact ◽  
Total P ◽  
Better Than ◽  
P Removal

Two species of macrophytes, Baumea articulata and Schoenoplectus mucronatus, were examined for their capacity to remove phosphorus under nutrient-rich conditions. Forty large bucket systems with the two different species growing in two types of substrate received artificial wastewaters for nine months, simulating a constructed wetland (CW) under high loading conditions. Half of the plants growing in the topsoil and gravel substrates were periodically harvested whereas the other half remained intact. Plant tissue and substrate samples were regularly analysed to determine their phosphorus concentrations. With respect to phosphorus uptake and removal, the Schoenoplectus in the topsoil medium performed better than the Baumea. Biomass harvesting enhanced P uptake in the Schoenoplectus, however the effect was not significant enough to make an improvement on the overall P removal, due to the slow recovery of plants and regrowth of biomass after harvesting. From P partitioning, it was found that the topsoil medium was the major P pool, storing most of total P present in the system. Plant parts contributed only minor storage with approximately half of that P stored below ground in the plant roots. The overall net effect of harvesting plant biomass was to only remove less than 5% of total phosphorus present in the system.

scholarly journals Water and phosphorus uptake by upland rice root systems unraveled under multiple scenarios: linking a 3D soil-root model and data

2020 ◽  
Author(s):  
Trung Hieu Mai ◽  
Pieterjan De Bauw ◽  
Andrea Schnepf ◽  
Roel Merckx ◽  
Erik Smolders ◽  
...  
Keyword(s):  
Upland Rice ◽  
Phosphorus Uptake ◽  
Root Systems ◽  
Multiscale Model ◽  
P Uptake ◽  
Water Dynamics ◽  
Small Scale ◽  
P Availability ◽  
P Deficiency ◽  
Plant P Uptake

AbstractBackground and aimsUpland rice is often grown where water and phosphorus (P) are limited and these two factors interact on P bioavailability. To better understand this interaction, mechanistic models representing small-scale nutrient gradients and water dynamics in the rhizosphere of full-grown root systems are needed.MethodsRice was grown in large columns using a P-deficient soil at three different P supplies in the topsoil (deficient, suboptimal, non-limiting) in combination with two water regimes (field capacity versus drying periods). Root architectural parameters and P uptake were determined. Using a multiscale model of water and nutrient uptake, in-silico experiments were conducted by mimicking similar P and water treatments. First, 3D root systems were reconstructed by calibrating an architecure model with observed phenological root data, such as nodal root number, lateral types, interbranch distance, root diameters, and root biomass allocation along depth. Secondly, the multiscale model was informed with these 3D root architectures and the actual transpiration rates. Finally, water and P uptake were simulated.Key resultsThe plant P uptake increased over threefold by increasing P and water supply, and drying periods reduced P uptake at high but not at low P supply. Root architecture was significantly affected by the treatments. Without calibration, simulation results adequately predicted P uptake, including the different effects of drying periods on P uptake at different P levels. However, P uptake was underestimated under P deficiency, a process likely related to an underestimated affinity of P uptake transporters in the roots. Both types of laterals (i.e. S- and L-type) are shown to be highly important for both water and P uptake, and the relative contribution of each type depend on both soil P availability and water dynamics. Key drivers in P uptake are growing root tips and the distribution of laterals.ConclusionsThis model-data integration demonstrates how multiple co-occurring single root phene responses to environmental stressors contribute to the development of a more efficient root system. Further model improvements such as the use of Michaelis constants from buffered systems and the inclusion of mycorrhizal infections and exudates are proposed.

PHOSPHORUS UPTAKE BY ALFALFA AS INFLUENCED BY PHOSPHATE SOURCE AND MOISTURE

1962 ◽  
Vol 42 (2) ◽  
pp. 254-265 ◽  
Author(s):  
J. D. Beaton ◽  
D. W. L. Read ◽  
W. C. Hinman
Keyword(s):  
Phosphorus Uptake ◽  
Significant Negative Correlation ◽  
P Uptake ◽  
Water Soluble ◽  
Ammonium Polyphosphate ◽  
Short Term ◽  
Treated Soil ◽  
Extractable P ◽  
Phosphate Source ◽  
Soil Fertilizer

The effect of phosphate source and soil moisture during the initial soil-fertilizer reaction period on subsequent phosphorus uptake by alfalfa was investigated in a growth chamber. Phosphate-treated soils with moisture adjusted to four different tensions were stored at approximately 18 °C. for 10 weeks. Following this storage interval phosphorus uptake by alfalfa was measured using a short-term technique.Phosphorus content and phosphorus uptake by both tops and roots increased significantly when water-soluble materials such as ammonium polyphosphate, monoammonium and monocalcium phosphate were applied. Less soluble sources, i.e., hydroxyapatite and anhydrous dicalcium phosphate, were much less effective. Calcium metaphosphate produced intermediate results.Moisture content of the soil during the reaction period did not greatly alter subsequent P uptake. The water-soluble sources of phosphorus were affected to the greatest degree.Uptake of P was significantly correlated with the amount of P extracted by NaHCO3 from the treated soils. The highest degree of correlation occurred with ammonium polyphosphate treated soil. A significant negative correlation occurred with calcium metaphosphate. With the exception of the 0.8 bar treatment, moisture tension had little influence on the correlation of P uptake with NaHCO3 extractable-P.

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

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