The Application of Aqueous Two-phase System in the Extraction of Natural Products from Chinese Herbal Medicine: A Review

2019 ◽  
Vol 23 (6) ◽  
pp. 704-720
Author(s):  
Cheng Xiang ◽  
Jie Chang ◽  
Ying Ying Yue ◽  
Ju Wang ◽  
Yan Fu
Keyword(s):  
Natural Products ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Extraction Efficiency ◽  
Extraction Methods ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
The Past ◽  
Chinese Herbal

Background: In the past decades, Chinese herbal medicine has attracted worldwide attention because they contain a variety of active ingredients which are beneficial to human health. As a result, there is a growing interest in the extraction of these substances. However, traditional extraction methods not only need a large amount of extractant, but are also time-consuming, moreover, the extraction efficiency is extremely poor and tedious purification steps are required to purify the crude extract. Thus, researchers hope to find an alternative method for the extraction of these components and the aqueous two-phase system (ATPS) seems to be one. Objective: This review focuses on introducing the properties of the aqueous two-phase system and summarizing the application of ATPS in the extraction of natural products. Meanwhile, this review also provided a guideline to researchers who wish to design a suitable ATPS for a specific target and how to amplify it to industrial-scale.

An aqueous two-phase system to pre-purify a heterologously produced siderophore

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (03) ◽  
pp. 135-138
Author(s):  
Mahmoud Kamal Ahmadi ◽  
Samar Fawaz ◽  
Blaine A. Pfeifer
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Cell Growth ◽  
Complex Nature ◽  
Phase System ◽  
Heterologous Production ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Purification System ◽  
Surrogate Host

Natural products span broad activities and applications; however, their access and production are often limited by native cellular sources. As a result, the heterologous production of a siderophore termed yersiniabactin (Ybt) was completed using the surrogate host Escherichia coli. Post-production and purification steps are complicated by the complex nature of most media used for cell growth, prompting the development in this work of an aqueous two-phase pre-purification system capable of rapidly and simply enhancing the concentration of the target Ybt compound.

Comparison of hydrophilic and hydrophobic deep eutectic solvents for pretreatment determination of sulfonamides from aqueous environments

2019 ◽  
Vol 11 (46) ◽  
pp. 5901-5909 ◽  
Author(s):  
Yuxin Ma ◽  
Qi Wang ◽  
Tao Zhu
Keyword(s):  
Water Samples ◽  
Extraction Efficiency ◽  
Deep Eutectic Solvents ◽  
Environmental Water ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Environments ◽  
Extraction Solvents

Two types of deep eutectic solvents were synthesized for an aqueous two-phase system to determine the sulfonamides from environmental water samples. The ChCl-Ph based ATPS showed good extraction efficiency. The method avoids use of a large number of extraction solvents.

scholarly journals Partitioning of Cephalexin in Ionic Liquid Aqueous Two-Phase System Composed of 1-Butyl-3-Methylimidazolium Tetrafluoroborate andZnSO4

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Fang Li ◽  
Juan Han ◽  
Yun Wang ◽  
Jing Jing Ma ◽  
Yong Sheng Yan
Keyword(s):  
Ionic Liquid ◽  
Extraction Efficiency ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Separation ◽  
Partitioning Behavior

Ionic liquid aqueous two-phase system (ILATPS) was applied in the extraction and separation of hydrosoluble antibiotics. The partitioning behavior of cephalexin (CEX) in 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4)-ZnSO4aqueous two-phase system was studied by the partitioning parameter of the extraction efficiency. The effect of the volume of [Bmim]BF4, the concentration of ZnSO4, temperature, pH, and the volume of ZnSO4solution was discussed concretely. When the volume of [Bmim]BF4was 2 mL and the concentration of ZnSO4was 35%, the extraction efficiency of CEX could reach 92.64% with pH unadjusted. The effect of the volume of [Bmim]BF4on the extraction efficiency was higher than that of the concentration of ZnSO4. The temperature influenced not only the formation of aqueous two-phase system but also the extraction efficiency of CEX. The target was found to be preferentially extracted to the [Bmim]BF4-rich phase at the pH below 4.3. The partition of CEX to the top phase was enhanced by increasing the volume of [Bmim]BF4, the concentration of ZnSO4, and temperature; however, the partition of CEX to the top phase increased by decreasing the pH.

scholarly journals Systematic Review on Chinese Herbal Medicine Induced Liver Injury

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Peng Zhang ◽  
Yongan Ye ◽  
Xianzhao Yang ◽  
Yuntao Jiao
Keyword(s):  
Systematic Review ◽  
Liver Transplantation ◽  
Herbal Medicine ◽  
Liver Injury ◽  
Latent Period ◽  
Chinese Herbal Medicine ◽  
Clinical Characteristics ◽  
Eligibility Criteria ◽  
The Past ◽  
Chinese Herbal

Background. In recent years, with the popularity of CHM, its hepatotoxicity has also been increasingly noticed. However, there are still veils on causative herbs and clinical characteristics.Aim. To systematically review data on CHM induced liver injury with particular focus on causative herbs and clinical characteristics.Methods. Using terms related to CHM and liver injury, PubMed and three Chinese electronic databases were searched, which was limited to the past 5 years. Publications meeting our eligibility criteria were included and further analyzed.Results. In total, 4 single herbs, 21 patent drugs, and 4 decoctions were reported to be of hepatotoxicity, with He-Shou-Wu being the most common one (65/114). Dang-Gui and other 5 herbs were the most common ingredients of patent drugs and decoctions. All patients were assessed using the RUCAM scale, with 26 being highly probable and 28 being probable. For these 54 cases, the latent period was 30 (47) days, and 81.48% were labeled as hepatocellular injuries. Most patients (96.3%) recovered, apart from the fact that one died and one is receiving liver transplantation.Conclusions. CHM should be used carefully for hepatotoxicity. Liver injury from CHM is similar to that from conventional medicines in clinical characteristics. Details about causative herbs should be illustrated, and more RUCAM should be used in future.

The effect of the chinese herbal medicine Dai-Kenchu-To on intestinal blood flow

2001 ◽  
Vol 120 (5) ◽  
pp. A248-A248
Author(s):  
N KAWASAKI ◽  
K NARIAI ◽  
M NAKAO ◽  
K NAKADA ◽  
N HANYUU ◽  
...  
Keyword(s):  
Blood Flow ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Intestinal Blood Flow ◽  
Chinese Herbal
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