Coumarin-based Fluorescent Probes for Bioimaging: Recent Applications and Developments

2021 ◽  
Vol 25 ◽  
Author(s):  
Jinhua Xie ◽  
Le Wang ◽  
Xiqi Su ◽  
João Rodrigues
Keyword(s):  
Fluorescent Probes ◽  
Photoinduced Electron Transfer ◽  
Metal Ion ◽  
Intramolecular Charge Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Biological Sensing ◽  
Ion Imaging ◽  
Future Perspectives

: Coumarin-based derivatives have easy modification and tunable properties and have been synthesized and applied in many areas including pro-drugs, biomedical materials, and chemical and biological sensing. In this review, recent advances of coumarin-based fluorescent probes for biosensing are reported for metal ion imaging. Metal detection in living cells will be highlighted with representative examples together with fluorescence response mechanisms such as photoinduced electron transfer (PET), intramolecular charge transfer (ICT), and fluorescence resonance energy transfer (FRET). Some future perspectives are also briefly mentioned.

scholarly journals Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells.

1986 ◽  
Vol 103 (4) ◽  
pp. 1221-1234 ◽  
Author(s):  
P S Uster ◽  
R E Pagano
Keyword(s):  
Energy Transfer ◽  
Fluorescent Probes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Model Membranes ◽  
Energy Donor ◽  
Membrane Bound ◽  
Energy Acceptor ◽  
In Cells

A conventional fluorescence microscope was modified to observe the sites of resonance energy transfer (RET) between fluorescent probes in model membranes and in living cells. These modifications, and the parameters necessary to observe RET between membrane-bound fluorochromes, are detailed for a system that uses N-4-nitrobenzo-2-oxa-1,3-diazole (NBD) or fluorescein as the energy donor and sulforhodamine as the energy acceptor. The necessary parameters for RET in this system were first optimized using liposomes. Both quenching of the energy donor and sensitized fluorescence of the energy acceptor could be directly observed in the microscope. RET microscopy was then used in cultured fibroblasts to identify those intracellular organelles labeled by the lipid probe, N-SRh-decylamine (N-SRh-C10). This was done by observing the sites of RET in cells doubly labeled with N-SRh-C10 and an NBD-labeled lipid previously shown to label the endoplasmic reticulum, mitochondria, and nuclear envelope. RET microscopy was also used in cells treated with fluorescein-labeled Lens culinaris agglutinin and a sulforhodamine derivative of phosphatidylcholine to examine the internalization of plasma membrane lipid and protein probes. After internalization, the fluorescent lectin resided in most, but not all of the intracellular compartments labeled by the fluorescent lipid, suggesting sorting of the membrane-bound lectin into a subset of internal compartments. We conclude that RET microscopy can co-localize different membrane-bound components at high resolution, and may be particularly useful in examining temporal and spatial changes in the distribution of fluorescent molecules in membranes of the living cell.

scholarly journals Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 470
Author(s):  
Pavel A. Panchenko ◽  
Anastasija V. Efremenko ◽  
Alexey V. Feofanov ◽  
Mariya A. Ustimova ◽  
Yuri V. Fedorov ◽  
...  
Keyword(s):  
Intramolecular Charge Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Fluorescence Intensity Ratio ◽  
Imaging Studies ◽  
Band Shape ◽  
Styryl Dye ◽  
Ratiometric Detection ◽  
Bio Imaging

Bis(styryl) dye 1 bearing N-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg2+ via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500–600 nm) to red (600–730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg2+ concentration in the 37 nM–1 μM range.

Various Sensing Mechanisms for the Design of Naphthalimide based Chemosensors Emerging in Recent Years

2020 ◽  
Vol 13 (4) ◽  
pp. 262-289
Author(s):  
Duraisamy Udhayakumari
Keyword(s):  
Metal Ions ◽  
Intramolecular Charge Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Intramolecular Proton Transfer ◽  
Biological Applications ◽  
Fluorescent Chemosensors ◽  
The Past ◽  
Photo Induced Electron Transfer ◽  
Displacement Approach

In the design of novel fluorescent chemosensors, investigation of new sensing mechanisms between recognition and signal reporting units is of increasing interest. In recent years, a smart chemosensor probe containing a 1,8-naphthalimide moiety could be developed as a fluorescent and colorimetric sensor for toxic anions, metal ions, biomolecules, nitroaromatics, and acids and be further applied to monitor the relevant biological applications. In this field, several problems and challenges still exist. This critical review is mainly focused on various sensing mechanisms that have emerged in the past few years, such as Photo-Induced Electron Transfer (PET), Intramolecular Charge Transfer (ICT), Fluorescence Resonance Energy Transfer (FRET), Excited-State Intramolecular Proton Transfer (ESIPT), hydrogen bonding and displacement approach. The review concludes with some current and future perspectives, including the use of the naphthalimides for sensing anions, metal ions, biomolecules, nitroaromatics and acids and their potential uses in various fields.

scholarly journals A Fluorescence Resonance Energy Transfer-Based Analytical Tool for Nitrate Quantification in Living Cells

ACS Omega ◽  
2020 ◽  
Vol 5 (46) ◽  
pp. 30306-30314
Author(s):  
Urooj Fatima ◽  
Fuad Ameen ◽  
Neha Soleja ◽  
Parvez Khan ◽  
Abobakr Almansob ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Analytical Tool ◽  
Fluorescence Resonance

scholarly journals Biological nanoscale fluorescent probes: From structure and performance to bioimaging

2020 ◽  
Vol 39 (1) ◽  
pp. 209-221
Author(s):  
Jiafeng Wan ◽  
Xiaoyuan Zhang ◽  
Kai Zhang ◽  
Zhiqiang Su
Keyword(s):  
Fluorescent Probes ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Biological Imaging ◽  
Fluorescent Materials ◽  
And Performance

Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.

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