Amelioration of Experimental Hepatotoxicity in Rats by Portulaca oleracea Linn. from Kashmir Himalaya

2021 ◽  
Vol 24 ◽  
Author(s):  
Mohd Akbar Dar ◽  
Prince Ahad Mir ◽  
Mubashir Hussain Masoodi ◽  
Ali S. Alqahtani ◽  
Nasir A. Siddiqui ◽  
...  
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Liver Diseases ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Portulaca Oleracea ◽  
Serum Glutamic Oxaloacetic Transaminase

Background: Traditionally, Portulaca oleracea Linn for treating abscesses, dysentery, and also for the treatment of liver diseases. Recent studies have reported its efficacy as neuroprotective, analgesic and anti-inflammatory, a bronchodilator, anticancer, antioxidant, wound healing, besides other important pharmacological actions. Aim and Objective: For curing liver diseases, Chinese use juice from the fresh leaves of Portulaca oleracea Linn, hence to prove this claim an in-vivo hepatoprotective study of extracts from Portulaca oleracea Linn. against carbon tetrachloride hepatotoxic rats. Methods: The in-vitro antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and hepatoprotective activity of extracts of Portulaca oleracea Linn. was assessed against the carbon tetrachloride-induced toxicity rat model. Results: The extracts of Portulaca oleracea Linn produced concentration-dependent percentage inhibition of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical and most significantly by methanol extract. Among the extracts, methanol extract showed a significant decrease in Serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) levels, and a significant increase in total protein (TP) levels against carbon tetrachloride-induced liver toxicity. Conclusion: The results obtained showed that methanol extract of Portulaca oleracea Linn. possesses significant DPPH free radical scavenging and hepatoprotective potential.

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF ARAUCARIA COLUMNARIS BARK PEEL AND COSMOS SULPHUREUS FLOWERS

2017 ◽  
Vol 9 (4) ◽  
pp. 96 ◽  
Author(s):  
Krishma M. Jadav ◽  
K. N. Ninge Gowda
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Phytochemical Composition ◽  
Dpph Method ◽  
In Vitro Antioxidant Activity

Objective: Four different extracts of Araucaria columnaris (bark peel) and Cosmos sulphureus (flowers) were screened for their phytochemical composition, and free radical scavenging activities.Methods: DPPH method was used to test the antioxidant activity for extracts.Results: Among the different extracts tested, the methanol extract of both the plant species showed significant radical scavenging activities. Phytochemical analysis of the extracts revealed that the radical scavenging activities might be due to the presence of flavonoids, tannins and phenolic compounds.Conclusion: The results obtained suggest that Araucaria columnaris (bark peel) and Cosmos sulphureus(flowers) could be exploited in the treatment of various diseases like cancer, cardiovascular diseases and infection diseases.  

scholarly journals Ameliorative effect of alkaloid extract of Cyclea peltata (Poir.) Hook. f. & Thoms. roots (ACP) on APAP/CCl4 induced liver toxicity in Wistar rats and in vitro free radical scavenging property

2014 ◽  
Vol 4 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Varghese Jancy Shine ◽  
Panikamparambil Gopalakrishnan Latha ◽  
Somasekharan Nair Rajam Suja ◽  
Gangadharan Indira Anuja ◽  
Gopan Raj ◽  
...  
Keyword(s):  
Free Radical ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Ameliorative Effect

scholarly journals IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF ARISTOLOCHIA TAGALA

2018 ◽  
Vol 10 (4) ◽  
pp. 40 ◽  
Author(s):  
Khetbadei Lysinia Hynniewta Hadem ◽  
Arnab Sen
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Fraction I

Objective: Study of antioxidant activity of a crude aqueous-methanol extract of Aristolochia tagala and its fractions and identification of the compounds with antioxidant activity.Methods: The antioxidant activity was assayed by the ability to scavenge free radicals such as superoxide, nitric oxide and ABTS radical cation and the identification of compounds was carried out by LC/MS analysis.Results: Fraction I of Aristolochia tagala showed the highest free radical scavenging activity and compounds responsible for its activity were identified as magnoflorine, apigenin dimethyl ether, aristolone, and N-trans-feruloyldopamine.Conclusion: The free radical scavenging property of the compounds present in Aristolochia tagala may be one mechanism that contributed to medicinal property exhibited by this plant.

scholarly journals Moringa oleifera Leaf Extract Promotes Antioxidant, Survival, Fecundity, and Locomotor Activities in Drosophila melanogaster

2020 ◽  
pp. 30-42
Author(s):  
Walter Mdekera Iorjiim ◽  
Simeon Omale ◽  
Great David Bagu ◽  
Steven Samuel Gyang ◽  
Emmanuel Taiwo Alemika
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Free Radical ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Thiol Level

Aim: The study was designed to investigate antioxidant, survivability,  fecundity, and locomotor activity of Moringa oleifera leaf (MOL) extract in Drosophila melanogaster. Materials and Methods: The study was conducted at the Africa Centre of Excellence in phytomedicine Research and Development (ACEPRD), University of Jos, Nigeria, in August 2019 - March 2020. In the first place, in vitro analysis of the antioxidant activity of extracts of M. oleifera in various solvents (Aqueous, Methanol-Aqueous co-solvent (80 % v/v) and n-Hexane) were evaluated using DPPH (1,1-Diphenyl-2-Picrylhydrazyl) assay. Based on the in vitro result, the methanol extract with the best free radical scavenging activity was used in graded doses for conducting the in vivo studies, and the observations were recorded. Distilled water (1000 µL) was used alone in 10 g fly food (as negative control) or as a solvent to dissolve MOL extract or ascorbic acid (positive control) separately before mixing with the fly food.  Statistical significance was taken at P<0.05 Results: The methanol extract of M. oleifera leaf (MEMOL) showed significantly (P<0.05) higher free radical scavenging ability (IC50 = 60 µg/ml) compared with the aqueous (IC50 = 100 µg/ml) and n-hexane (IC50 = 250 µg/ml) extracts respectively. The median lethal dose (LD50) of MEMOL was >2000 mg. Supplementation with MEMOL non-significantly (P>0.05) improved movement, significantly (P<0.05) increased survivability, fecundity, and total thiol level. The activities of glutathione-S-transferase (GST) and catalase (CAT) significantly (P<0.05) increased. The superoxide dismutase (SOD) activity non-significantly (P>0.05) decreased, while malondialdehyde (MDA) concentration decreased significantly (P<0.05) compared with controls, respectively. Conclusion:  In vitro study suggested better antioxidant activity of MEMOL. In vivo study also revealed that MEMOL was relatively safe in D. melanogaster, supported by high LD50, increased survivability, fecundity, locomotor ability, antioxidant enzyme activities, total thiol level, along with a concomitant decrease in MDA content.

Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sridevi Chigurupati ◽  
Shantini Vijayabalan ◽  
Kesavanarayanan Krishnan Selvarajan ◽  
Ahmad Alhowail ◽  
Fatema Kauser
Keyword(s):  
Free Radical ◽  
Crude Extract ◽  
Leucaena Leucocephala ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
16S Rrna Gene Sequencing ◽  
Free Radical Scavenging ◽  
Rrna Gene ◽  
Standard Drug

AbstractObjectivesResearch on endosymbionts is emerging globally and is considered as a potential source of bioactive phytochemicals. The present study examines the antioxidant and antidiabetic of the endophytic crude extract isolated from Leucaena leucocephala leaves.MethodsEndophytic bacteria were isolated from the leaves of L. leucocephala and 16S rRNA gene sequencing was used to establish their identity. The in vitro antioxidant effect of endophytic crude extract (LL) was evaluated using 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical scavenging methods. The in vitro antidiabetic properties of LL were evaluated using α-amylase and α-glucosidase enzyme inhibition assay.ResultsThe isolated endophytic bacteria were identified as Cronobacter sakazakii. LL displayed potent free radical scavenging effect against ABTS and DPPH radicals with an inhibitory concentration 50% (IC50) value of 17.49 ± 0.06 and 11.3 ± 0.1 μg/mL respectively. LL exhibited α-amylase and α-glucosidase inhibition with an IC50 value of 23.3 ± 0.08 and 23.4 ± 0.1 μg/mL respectively compared to the standard drug (acarbose). Both glucose loaded normoglycemic rats and STZ induced diabetic rats treated with LL (200 mg/kg) exhibited a considerable reduction in blood glucose levels p<0.01 after 8 h of treatment when compared to normal and diabetic control rats respectively.ConclusionsThus, the study shows that LL has a wellspring of natural source of antioxidants, and antidiabetic agents and phytoconstituents present in endophytes could be the rich source for bioactive compounds.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

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