Effects of Montmorillonite on Growth Performance, Serum Biochemistry and Oxidative Stress of Red-Crowned Crane (Grus japonensis) Fed Mycotoxin-Contaminated Feed

Background: The red-crowned crane (Grus japonensis) is one of the most vulnerable bird species in the world. Mycotoxins are toxic secondary metabolites produced by fungi and considered naturally unavoidable contaminants in animal feed. Our recent survey indicated that the mycotoxins had the potential to contaminate redcrowned crane’s regular diets in China. Objective: This experiment was conducted to investigate the protective effects of mycotoxin binder montmorillonite (Mont) on growth performance, serum biochemistry and oxidative stress parameters of the red-crowned crane. Methods: 16 red-crowned cranes were divided into four groups and fed one of the following diets; a selected diet, regular diet, or the selected diet or regular diet with 0.5% montmorillonite added to the diets. The cranes' parameters of performance, hematology, serum biochemistry and serum oxidative stress were measured. Results: Consuming regular diets decreased the average daily feed intake (ADFI), levels of haemoglobin (Hb), platelet count (PLT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), but increased the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH). The supplementation of 0.5% Mont provided protection for the red-crowned crane in terms of feed intake, serum biochemistry and oxidative stress. Moreover, Mont supplementation had no adverse effect on the health of red-crowned crane. Conclusions: Taken together, these findings suggested that the addition of dietary Mont is effective in improving the health of red-crowned crane.

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Red-Crowned Crane (Grus japonensis) Reproduction Was Improved by Inhibiting Mycotoxins with Montmorillonite in Feed

Toxins ◽

10.3390/toxins12030191 ◽

2020 ◽

Vol 12 (3) ◽

pp. 191 ◽

Cited By ~ 1

Author(s):

Dawei Liu ◽

Chao Gu ◽

Changhu Lu ◽

Qinghua Wu ◽

Kamil Kuca ◽

...

Keyword(s):

Shell Thickness ◽

Egg Quality ◽

Animal Feed ◽

Bird Species ◽

Breeding Period ◽

Egg Weight ◽

Testosterone Concentration ◽

Regular Diet ◽

Grus Japonensis ◽

Mating Behaviors

The red-crowned crane (Grus japonensis) is a vulnerable bird species. Mycotoxins are toxic substances produced by filamentous fungi and are considered as naturally unavoidable contaminants in animal feed. Our recent survey indicated that feeds designed for captive red-crowned cranes were contaminated with mycotoxins. This study was conducted to investigate the protective effects of the mycotoxin binder montmorillonite on the reproductive behavior, sex hormone levels, and egg quality of red-crowned cranes. Twelve pairs of G. japonensis were divided into four groups, and each group was fed one of the following: a selected diet (with extra low levels of mycotoxins), a regular diet, a selected diet with 0.5% montmorillonite added, or a regular diet with 0.5% montmorillonite added. Consumption of the regular diet decreased courtship and mating behaviors, testosterone concentration, egg weight, and shell thickness. However, feed supplementation with montmorillonite increased the courtship, mating behaviors and testosterone concentration during the pre-breeding period, as well as egg weight and shell thickness. These findings suggest that the addition of dietary montmorillonite is effective for controlling mycotoxins in the feed, resulting in improvements in reproductive behaviors, testosterone concentrations, and some egg quality parameters of the red-crowned crane.

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Effects of conjugated linoleic acid and curcumin on growth performance and oxidative stress enzymes in juvenile Pacific white shrimp ( Litopenaeus vannamei ) feed with aflatoxins

Aquaculture Research ◽

10.1111/are.14451 ◽

2019 ◽

Vol 51 (3) ◽

pp. 1051-1060

Author(s):

Oscar Daniel García‐Pérez ◽

Mireya Tapia‐Salazar ◽

Martha G. Nieto‐López ◽

Julio César Cruz‐Valdez ◽

Maribel Maldonado-Muñiz ◽

...

Keyword(s):

Oxidative Stress ◽

Linoleic Acid ◽

Growth Performance ◽

Conjugated Linoleic Acid ◽

Litopenaeus Vannamei ◽

Pacific White Shrimp ◽

White Shrimp ◽

Oxidative Stress Enzymes ◽

Stress Enzymes ◽

And Oxidative Stress

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Protective effects of astaxanthin from Haematococcus pluvialis on the survival and oxidative stress of zebrafish embryos induced by microcystin-LR

Journal of Applied Phycology ◽

10.1007/s10811-021-02448-6 ◽

2021 ◽

Author(s):

Jing Dong ◽

Chenlu Li ◽

Dujuan Dai ◽

Man Zhang ◽

Yunni Gao ◽

...

Keyword(s):

Oxidative Stress ◽

Haematococcus Pluvialis ◽

Zebrafish Embryos ◽

Protective Effects ◽

And Oxidative Stress

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Effects of Dietary β-Mannanase Supplementation on Growth Performance, Apparent Total Tract Digestibility, Intestinal Integrity, and Immune Responses in Weaning Pigs

Animals ◽

10.3390/ani10040703 ◽

2020 ◽

Vol 10 (4) ◽

pp. 703

Author(s):

Jae-Cheol Jang ◽

Kwang Kim ◽

Young Jang ◽

Yoo Kim

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Growth Performance ◽

Basal Diet ◽

Villus Height ◽

Crypt Depth ◽

Intestinal Integrity ◽

Weaning Pigs ◽

Dietary Treatments ◽

And Oxidative Stress

The experiment aimed to investigate the effects of dietary β-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of nutrients, intestinal integrity, and the immunological and oxidative stress parameters in weaning pigs. A total of 64 newly weaning pigs (initial body weight: 6.96 ± 0.70 kg) were allotted to two dietary treatments in eight replicates per treatment with four pigs per pen based on body weight and sex. Dietary treatments were 1.) CON (control: corn-soybean meal based basal diet) and 2.) β-mannanase (basal diet +0.06% β-mannanase). The β-mannanase supplementation did not affect growth performance, concentrations of acute phase protein, superoxide dismutase and glutathione peroxidase. However, the pigs fed the β-mannanase-supplemented diet had greater ATTD of ether extract, jejunum villus height, and villus height-to-crypt depth ratio, and lower crypt depth compared with those fed the CON diet (p < 0.05). The pigs fed the β-mannanase-supplemented diet tended to have the lower count of E. coli in cecum than those fed the CON diet (p = 0.08). In conclusion, dietary β-mannanase supplementation did not affect growth performance, immune response and oxidative stress of weaning pigs, whereas it increased fat digestibility and had positive effects on intestinal integrity and cecum microflora by reducing the count of E.coli.

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The protective effects of carvacrol and thymol against paracetamol–induced toxicity on human hepatocellular carcinoma cell lines (HepG2)

Human & Experimental Toxicology ◽

10.1177/0960327115627688 ◽

2016 ◽

Vol 35 (12) ◽

pp. 1252-1263 ◽

Cited By ~ 23

Author(s):

SS Palabiyik ◽

E Karakus ◽

Z Halici ◽

E Cadirci ◽

Y Bayir ◽

...

Keyword(s):

Oxidative Stress ◽

Inflammatory Cytokines ◽

Hepg2 Cells ◽

Folk Medicine ◽

Hepatoprotective Activity ◽

High Dose ◽

Protective Effects ◽

And Oxidative Stress ◽

Pro Inflammatory Cytokines

Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 µM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol.

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246 Effects of Feeding Thermally Processed Spray-dried Egg Whites on Growth Performance, Apparent Digestibility, and Oxidative Stress in Nursery Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.147 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 91-92

Author(s):

Victoria C Wilson ◽

Brian J Kerr

Keyword(s):

Oxidative Stress ◽

Growth Performance ◽

Liver Tissue ◽

Protein Carbonyls ◽

Nursery Pigs ◽

Thermally Processed ◽

Markers Of Oxidative Stress ◽

Dietary Treatments ◽

And Oxidative Stress ◽

Spray Dried

Abstract The objectives of this study were to determine if feeding thermally processed (TP, heated at 100°C for 120 h) spray-dried egg whites (SDEW) to nursery pigs would impact growth performance, apparent total tract digestibility (ATTD) of GE, N, and S, and oxidative stress. Thirty-two barrows, (initial BW 7.1 kg) were randomly assigned to dietary treatments with 1 pig per pen. In a preliminary study, thermally processing SDEW at 100°C for 120 h increased protein carbonyls (PC) from 6 µmol/g to 19.4 µmol/g (P ≤ 0.01). Diets included either 12% SDEW, 6% TP-SDEW plus 6% SDEW, or 12% TP-SDEW. The experiment lasted 24 d for collection of growth performance data, while plasma was collected on d 21 and liver tissue harvested on d 24 to analyze for markers of oxidative stress. Feces were collected on d 22 for measures of ATTD. Daily gain, daily feed intake, feed efficiency, and ATTD of GE were not found to be different among dietary treatments (P ≥ 0.57). In contrast, ATTD of N (P = 0.11) and S (P = 0.03) were found to increase with increasing protein oxidation in the diet. There was no change in the plasma or liver F2-isoprostanes and 8-hydroxy-2’-deoxyguanosine among dietary treatments (P ≥ 0.28). An increase in plasma PC (P = 0.02) was observed in pigs fed 12% TP-SDEW compared to pigs fed 12% SDEW and pigs fed 6% TP-SDEW. In contrast, a decrease in liver tissue PC (P = 0.04) was observed in pigs fed 6% TP-SDEW compared to pigs fed 12% SDEW and 12% TP-SDEW. These results indicate that feeding TP-SDEW does not affect growth performance, ATTD of GE, and oxidative stress as indicated by F2-isoprostanes or 8-hydroxy-2’-deoxyguanosine; but appeared to have variable effects on oxidative stress as measured by PC.

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The protective effects of Δ9 -tetrahydrocannabinol against inflammation and oxidative stress in rat liver with fructose-induced hyperinsulinemia

Journal of Pharmacy and Pharmacology ◽

10.1111/jphp.13042 ◽

2018 ◽

Vol 71 (3) ◽

pp. 408-416 ◽

Cited By ~ 1

Author(s):

Alisa Bahar Beydogan ◽

Zeynep Mine Coskun ◽

Sema Bolkent

Keyword(s):

Oxidative Stress ◽

Rat Liver ◽

Protective Effects ◽

And Oxidative Stress

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Protective effects of anthocyanin against apoptosis and oxidative stress induced by arsanilic acid in DF-1 cells

Molecular Biology Reports ◽

10.1007/s11033-018-4472-5 ◽

2018 ◽

Vol 46 (1) ◽

pp. 301-308 ◽

Cited By ~ 3

Author(s):

Ning Xie ◽

Na Geng ◽

Dong Zhou ◽

Yuliang Xu ◽

Kangping Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effects ◽

Arsanilic Acid ◽

And Oxidative Stress

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Lysophosphatidylcholine Offsets the Protective Effects of Bone Marrow Mesenchymal Stem Cells on Inflammatory Response and Oxidative Stress Injury of Retinal Endothelial Cells via TLR4/NF-κB Signaling

Journal of Immunology Research ◽

10.1155/2021/2389029 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Haijun Zhao ◽

Yanhui He

Keyword(s):

Oxidative Stress ◽

Stem Cells ◽

Bone Marrow ◽

Endothelial Cells ◽

Inflammatory Response ◽

Protective Effects ◽

Stress Injury ◽

Oxidative Stress Injury ◽

Marrow Mesenchymal Stem Cells ◽

And Oxidative Stress

Diabetic retinopathy (DR), as a major cause of blindness worldwide, is one common complication of diabetes mellitus. Inflammatory response and oxidative stress injury of endothelial cells play significant roles in the pathogenesis of DR. The study is aimed at investigating the effects of lysophosphatidylcholine (LPC) on the dysfunction of high glucose- (HG-) treated human retinal microvascular endothelial cells (HRMECs) after being cocultured with bone marrow mesenchymal stem cells (BMSCs) and the underlying regulatory mechanism. Coculture of BMSCs and HRMECs was performed in transwell chambers. The activities of antioxidant-related enzymes and molecules of oxidative stress injury and the contents of inflammatory cytokines were measured by ELISA. Flow cytometry analyzed the apoptosis of treated HRMECs. HRMECs were further treated with 10-50 μg/ml LPC to investigate the effect of LPC on the dysfunction of HRMECs. Western blotting was conducted to evaluate levels of TLR4 and p-NF-κB proteins. We found that BMSCs alleviated HG-induced inflammatory response and oxidative stress injury of HRMECs. Importantly, LPC offsets the protective effects of BMSCs on inflammatory response and oxidative stress injury of HRMECs. Furthermore, LPC upregulated the protein levels of TLR4 and p-NF-κB, activating the TLR4/NF-κB signaling pathway. Overall, our study demonstrated that LPC offsets the protective effects of BMSCs on inflammatory response and oxidative stress injury of HRMECs via TLR4/NF-κB signaling.

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